(Linear) (Six-base) MAPSORT of: KIEE4379.seq  Check: 5989  from: 1  to: 1197



KIEE4379.orf

 With 182 enzymes: SgfI * 

                        December 2, 2013 11:07  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     167    1136    1197 
   Size:        167     969      61

  Fragments arranged by size: 

                 969     167      61
 
AflII C'TTAA_G

Cuts at:      0     349    1197 
   Size:        349     848
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1015    1025    1197 
   Size:       1015      10     172

  Fragments arranged by size: 

                1015     172      10
 
ApoI r'AATT_y

Cuts at:      0     580     847    1197 
   Size:        580     267     350

  Fragments arranged by size: 

                 580     350     267
 
AvaI C'yCGr_G

Cuts at:      0     875    1197 
   Size:        875     322
 
BamHI G'GATC_C

Cuts at:      0     800    1197 
   Size:        800     397
 
BanII G_rGCy'C

Cuts at:      0     671    1197 
   Size:        671     526
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     173     434     491     964    1197 
   Size:        173     261      57     473     233

  Fragments arranged by size: 

                 473     261     233     173      57
 
BbvCI CC'TCA_GC

Cuts at:      0    1064    1197 
   Size:       1064     133
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     325     427     960    1197 
   Size:        325     102     533     237

  Fragments arranged by size: 

                 533     325     237     102
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     874    1093    1197 
   Size:        874     219     104

  Fragments arranged by size: 

                 874     219     104
 
Bpu10I CC'TnA_GC

Cuts at:      0    1064    1197 
   Size:       1064     133
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     250    1197 
   Size:        250     947
 
BsaBI GATnn'nnATC

Cuts at:      0    1049    1197 
   Size:       1049     148
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     718    1081    1197 
   Size:        718     363     116

  Fragments arranged by size: 

                 718     363     116
 
Bsp1286I G_dGCh'C

Cuts at:      0     671    1197 
   Size:        671     526
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     120    1197 
   Size:        120    1077
 
BsrDI GCAATG_nn'

Cuts at:      0     671    1197 
   Size:        671     526
 
BsrFI r'CCGG_y

Cuts at:      0     174    1197 
   Size:        174    1023
 
BstBI TT'CG_AA

Cuts at:      0     473    1197 
   Size:        473     724
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     315    1197 
   Size:        315     882
 
BstYI r'GATC_y

Cuts at:      0     417     800     857    1197 
   Size:        417     383      57     340

  Fragments arranged by size: 

                 417     383     340      57
 
DraI TTT'AAA

Cuts at:      0     137    1197 
   Size:        137    1060
 
DraIII CAC_nnn'GTG

Cuts at:      0     810    1197 
   Size:        810     387
 
EarI CTCTTCn'nnn_

Cuts at:      0      53     695     820    1154    1197 
   Size:         53     642     125     334      43

  Fragments arranged by size: 

                 642     334     125      53      43
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     167     874    1093    1136    1197 
   Size:        167     707     219      43      61

  Fragments arranged by size: 

                 707     219     167      61      43
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      48     865    1197 
   Size:         48     817     332

  Fragments arranged by size: 

                 817     332      48
 
EcoO109I rG'GnC_Cy

Cuts at:      0     246    1197 
   Size:        246     951
 
EcoRI G'AATT_C

Cuts at:      0     847    1197 
   Size:        847     350
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      82     319    1197 
   Size:         82     237     878

  Fragments arranged by size: 

                 878     237      82
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     163     195    1197 
   Size:        163      32    1002

  Fragments arranged by size: 

                1002     163      32
 
HincII GTy'rAC

Cuts at:      0     642    1197 
   Size:        642     555
 
HindIII A'AGCT_T

Cuts at:      0     301    1197 
   Size:        301     896
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     234    1081    1197 
   Size:        234     847     116

  Fragments arranged by size: 

                 847     234     116
 
MspA1I CmG'CkG

Cuts at:      0     391     556    1197 
   Size:        391     165     641

  Fragments arranged by size: 

                 641     391     165
 
NdeI CA'TA_TG

Cuts at:      0      81    1197 
   Size:         81    1116
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     810     991    1197 
   Size:        810     181     206

  Fragments arranged by size: 

                 810     206     181
 
PfoI T'CCnGG_A

Cuts at:      0     851    1197 
   Size:        851     346
 
PpuMI rG'GwC_Cy

Cuts at:      0     246    1197 
   Size:        246     951
 
PvuII CAG'CTG

Cuts at:      0     391     556    1197 
   Size:        391     165     641

  Fragments arranged by size: 

                 641     391     165
 
SfcI C'TryA_G

Cuts at:      0     263    1197 
   Size:        263     934
 
SmaI CCC'GGG

Cuts at:      0     877    1197 
   Size:        877     320
 
SmlI C'TyrA_G

Cuts at:      0     229     349    1197 
   Size:        229     120     848

  Fragments arranged by size: 

                 848     229     120
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     110    1197 
   Size:        110    1087
 
Tth111I GACn'n_nGTC

Cuts at:      0    1021    1197 
   Size:       1021     176
 
XmaI C'CCGG_G

Cuts at:      0     875    1197 
   Size:        875     322
 
XmnI GAAnn'nnTTC

Cuts at:      0     121     961    1197 
   Size:        121     840     236

  Fragments arranged by size: 

                 840     236     121
 

 Enzymes that do cut:

     AcuI    AflII    AlwNI     ApoI     AvaI    BamHI    BanII     BbsI
    BbvCI     BmrI     BpmI   Bpu10I    BpuEI    BsaBI    BseRI Bsp1286I
    BspMI    BsrDI    BsrFI    BstBI    BstXI    BstYI     DraI   DraIII
     EarI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI    Hin4I   HincII
  HindIII     MmeI   MspA1I     NdeI    PflMI     PfoI    PpuMI    PvuII
     SfcI     SmaI     SmlI    TaqII  Tth111I     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI     BanI     BbeI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmtI     BplI
     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrGI
   BssHII    BssSI   BstAPI   BstEII  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DrdI     EaeI     EagI     EciI  EcoICRI
    EcoRV     FseI     FspI    FspAI    HaeII     HpaI     KasI     KpnI
     MfeI     MluI     MscI     MslI     NaeI     NarI     NcoI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI     TatI
     TstI     XbaI     XcmI     XhoI     ZraI