Restriction map for
pF1KE4483
(Linear) (Six-base) MAPSORT of: KIEE4483.seq Check: 9477 from: 1 to: 1500
KIEE4483.orf
With 182 enzymes: SgfI *
November 17, 2015 09:53 ..
AccI GT'mk_AC
Cuts at: 0 1199 1500
Size: 1199 301
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1050 1500
Size: 1050 450
AflIII A'CryG_T
Cuts at: 0 667 1500
Size: 667 833
AleI CACnn'nnGTG
Cuts at: 0 802 1500
Size: 802 698
AlwNI CAG_nnn'CTG
Cuts at: 0 1143 1500
Size: 1143 357
ApaI G_GGCC'C
Cuts at: 0 590 1500
Size: 590 910
ApaLI G'TGCA_C
Cuts at: 0 888 1500
Size: 888 612
AvaI C'yCGr_G
Cuts at: 0 478 1013 1500
Size: 478 535 487
Fragments arranged by size:
535 487 478
BamHI G'GATC_C
Cuts at: 0 576 1500
Size: 576 924
BanII G_rGCy'C
Cuts at: 0 64 590 1097 1433 1500
Size: 64 526 507 336 67
Fragments arranged by size:
526 507 336 67 64
BbsI GAAGACnn'nnnn_
Cuts at: 0 230 1056 1400 1500
Size: 230 826 344 100
Fragments arranged by size:
826 344 230 100
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1159 1193 1500
Size: 1159 34 307
Fragments arranged by size:
1159 307 34
Bme1580I G_kGCm'C
Cuts at: 0 590 892 1500
Size: 590 302 608
Fragments arranged by size:
608 590 302
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 641 1272 1500
Size: 641 631 228
Fragments arranged by size:
641 631 228
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 51 1479 1500
Size: 51 1428 21
Fragments arranged by size:
1428 51 21
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 702 1500
Size: 702 798
BsaI GGTCTCn'nnnn_
Cuts at: 0 1214 1500
Size: 1214 286
BsaAI yAC'GTr
Cuts at: 0 519 1500
Size: 519 981
BsaBI GATnn'nnATC
Cuts at: 0 1054 1500
Size: 1054 446
BsaHI Gr'CG_yC
Cuts at: 0 344 1500
Size: 344 1156
BsaWI w'CCGG_w
Cuts at: 0 627 1500
Size: 627 873
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 503 533 1420 1450 1500
Size: 503 30 887 30 50
Fragments arranged by size:
887 503 50 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1222 1500
Size: 1222 278
BseYI C'CCAG_C
Cuts at: 0 1367 1500
Size: 1367 133
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 289 310 1500
Size: 289 21 1190
Fragments arranged by size:
1190 289 21
BsiHKAI G_wGCw'C
Cuts at: 0 892 1433 1500
Size: 892 541 67
Fragments arranged by size:
892 541 67
BsmI GAATG_Cn'
Cuts at: 0 1182 1500
Size: 1182 318
Bsp1286I G_dGCh'C
Cuts at: 0 64 590 892 1097 1433 1500
Size: 64 526 302 205 336 67
Fragments arranged by size:
526 336 302 205 67 64
BspEI T'CCGG_A
Cuts at: 0 627 1500
Size: 627 873
BsrBI CCG'CTC
Cuts at: 0 284 1164 1500
Size: 284 880 336
Fragments arranged by size:
880 336 284
BsrDI GCAATG_nn'
Cuts at: 0 431 1500
Size: 431 1069
BsrGI T'GTAC_A
Cuts at: 0 1387 1500
Size: 1387 113
BssSI C'ACGA_G
Cuts at: 0 313 1500
Size: 313 1187
BstXI CCAn_nnnn'nTGG
Cuts at: 0 420 1500
Size: 420 1080
BstYI r'GATC_y
Cuts at: 0 576 917 1232 1500
Size: 576 341 315 268
Fragments arranged by size:
576 341 315 268
EaeI y'GGCC_r
Cuts at: 0 204 521 1500
Size: 204 317 979
Fragments arranged by size:
979 317 204
EcoICRI GAG'CTC
Cuts at: 0 1431 1500
Size: 1431 69
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 51 1050 1479 1500
Size: 51 999 429 21
Fragments arranged by size:
999 429 51 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1279 1500
Size: 1279 221
EcoO109I rG'GnC_Cy
Cuts at: 0 171 1253 1500
Size: 171 1082 247
Fragments arranged by size:
1082 247 171
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 179 211 1500
Size: 179 32 1289
Fragments arranged by size:
1289 179 32
HincII GTy'rAC
Cuts at: 0 363 846 1500
Size: 363 483 654
Fragments arranged by size:
654 483 363
MfeI C'AATT_G
Cuts at: 0 779 1500
Size: 779 721
MluI A'CGCG_T
Cuts at: 0 667 1500
Size: 667 833
MslI CAynn'nnrTG
Cuts at: 0 802 1348 1500
Size: 802 546 152
Fragments arranged by size:
802 546 152
MspA1I CmG'CkG
Cuts at: 0 1371 1500
Size: 1371 129
NdeI CA'TA_TG
Cuts at: 0 786 1500
Size: 786 714
NspI r_CATG'y
Cuts at: 0 1026 1500
Size: 1026 474
PflMI CCAn_nnn'nTGG
Cuts at: 0 754 1500
Size: 754 746
PmlI CAC'GTG
Cuts at: 0 519 1500
Size: 519 981
PpuMI rG'GwC_Cy
Cuts at: 0 171 1500
Size: 171 1329
PshAI GACnn'nnGTC
Cuts at: 0 130 1500
Size: 130 1370
PspOMI G'GGCC_C
Cuts at: 0 586 1500
Size: 586 914
PstI C_TGCA'G
Cuts at: 0 232 458 1500
Size: 232 226 1042
Fragments arranged by size:
1042 232 226
SacI G_AGCT'C
Cuts at: 0 1433 1500
Size: 1433 67
SfcI C'TryA_G
Cuts at: 0 228 454 501 1500
Size: 228 226 47 999
Fragments arranged by size:
999 228 226 47
SmaI CCC'GGG
Cuts at: 0 1015 1500
Size: 1015 485
SmlI C'TyrA_G
Cuts at: 0 681 1500
Size: 681 819
SphI G_CATG'C
Cuts at: 0 1026 1500
Size: 1026 474
StyI C'CwwG_G
Cuts at: 0 167 1500
Size: 167 1333
TatI w'GTAC_w
Cuts at: 0 1387 1500
Size: 1387 113
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 503 535 1500
Size: 503 32 965
Fragments arranged by size:
965 503 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 354 751 1191 1485 1500
Size: 354 397 440 294 15
Fragments arranged by size:
440 397 354 294 15
XmaI C'CCGG_G
Cuts at: 0 1013 1500
Size: 1013 487
Enzymes that do cut:
AccI AcuI AflIII AleI AlwNI ApaI ApaLI AvaI
BamHI BanII BbsI BcgI Bme1580I BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI Bsp1286I BspEI BsrBI BsrDI BsrGI
BssSI BstXI BstYI EaeI EcoICRI Eco57MI EcoNI EcoO109I
Hin4I HincII MfeI MluI MslI MspA1I NdeI NspI
PflMI PmlI PpuMI PshAI PspOMI PstI SacI SfcI
SmaI SmlI SphI StyI TatI TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI
BanI BbeI BbvCI BciVI BclI BfrBI BglI BglII
BlpI BmgBI BmrI BmtI Bpu10I BsiEI BsiWI BsmBI
BspHI BspMI BsrFI BssHII BstAPI BstBI BstEII BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EagI EarI EciI EcoRI EcoRV FalI FseI
FspI FspAI HaeII HindIII HpaI KasI KpnI MmeI
MscI NaeI NarI NcoI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PfoI PmeI PpiI PsiI
PspXI PsrI PvuI PvuII RsrII SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SnaBI SpeI SrfI SspI StuI SwaI TaqII Tth111I
XbaI XhoI XmnI ZraI