Restriction map for
pF1KE4526
(Linear) (Six-base) MAPSORT of: KIEE4526.seq Check: 5955 from: 1 to: 1722
KIEE4526.orf
With 182 enzymes: SgfI *
March 8, 2012 13:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 138 1722
Size: 138 1584
Acc65I G'GTAC_C
Cuts at: 0 926 1722
Size: 926 796
AfeI AGC'GCT
Cuts at: 0 202 1480 1722
Size: 202 1278 242
Fragments arranged by size:
1278 242 202
AhdI GACnn_n'nnGTC
Cuts at: 0 579 1722
Size: 579 1143
AleI CACnn'nnGTG
Cuts at: 0 1150 1722
Size: 1150 572
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1719 1722
Size: 1719 3
AlwNI CAG_nnn'CTG
Cuts at: 0 1439 1457 1509 1722
Size: 1439 18 52 213
Fragments arranged by size:
1439 213 52 18
ApaI G_GGCC'C
Cuts at: 0 36 426 1722
Size: 36 390 1296
Fragments arranged by size:
1296 390 36
ApaLI G'TGCA_C
Cuts at: 0 67 1603 1722
Size: 67 1536 119
Fragments arranged by size:
1536 119 67
AscI GG'CGCG_CC
Cuts at: 0 236 1722
Size: 236 1486
AvaI C'yCGr_G
Cuts at: 0 11 88 168 218 419 689 730 763
Size: 11 77 80 50 201 270 41 33
Cuts at: 763 883 952 1722
Size: 120 69 770
Fragments arranged by size:
770 270 201 120 80 77 69 50
41 33 11
BanI G'GyrC_C
Cuts at: 0 46 288 347 369 543 844 926 1722
Size: 46 242 59 22 174 301 82 796
Fragments arranged by size:
796 301 242 174 82 59 46 22
BanII G_rGCy'C
Cuts at: 0 36 100 175 274 322 426 460 1164
Size: 36 64 75 99 48 104 34 704
Cuts at: 1164 1516 1722
Size: 352 206
Fragments arranged by size:
704 352 206 104 99 75 64 48
36 34
BbeI G_GCGC'C
Cuts at: 0 50 292 547 1722
Size: 50 242 255 1175
Fragments arranged by size:
1175 255 242 50
BbvCI CC'TCA_GC
Cuts at: 0 197 1060 1722
Size: 197 863 662
Fragments arranged by size:
863 662 197
BfrBI ATG'CAT
Cuts at: 0 1486 1722
Size: 1486 236
BglI GCCn_nnn'nGGC
Cuts at: 0 1228 1722
Size: 1228 494
Bme1580I G_kGCm'C
Cuts at: 0 36 71 374 426 847 1291 1607 1722
Size: 36 35 303 52 421 444 316 115
Fragments arranged by size:
444 421 316 303 115 52 36 35
BmgBI CAC'GTC
Cuts at: 0 1575 1722
Size: 1575 147
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 231 1722
Size: 231 1491
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1000 1465 1722
Size: 1000 465 257
Fragments arranged by size:
1000 465 257
Bpu10I CC'TnA_GC
Cuts at: 0 197 1060 1510 1722
Size: 197 863 450 212
Fragments arranged by size:
863 450 212 197
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1459 1582 1722
Size: 1459 123 140
Fragments arranged by size:
1459 140 123
BsaI GGTCTCn'nnnn_
Cuts at: 0 477 902 1108 1722
Size: 477 425 206 614
Fragments arranged by size:
614 477 425 206
BsaAI yAC'GTr
Cuts at: 0 183 777 1608 1722
Size: 183 594 831 114
Fragments arranged by size:
831 594 183 114
BsaHI Gr'CG_yC
Cuts at: 0 47 279 289 544 1722
Size: 47 232 10 255 1178
Fragments arranged by size:
1178 255 232 47 10
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 442 472 1156 1183 1186 1213 1722
Size: 442 30 684 27 3 27 509
Fragments arranged by size:
684 509 442 30 27 27 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 456 718 1207 1722
Size: 456 262 489 515
Fragments arranged by size:
515 489 456 262
BseYI C'CCAG_C
Cuts at: 0 27 248 440 1268 1722
Size: 27 221 192 828 454
Fragments arranged by size:
828 454 221 192 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1174 1597 1722
Size: 1174 423 125
Fragments arranged by size:
1174 423 125
BsiEI CG_ry'CG
Cuts at: 0 191 653 1098 1722
Size: 191 462 445 624
Fragments arranged by size:
624 462 445 191
BsiHKAI G_wGCw'C
Cuts at: 0 71 1607 1722
Size: 71 1536 115
Fragments arranged by size:
1536 115 71
BsiWI C'GTAC_G
Cuts at: 0 959 1722
Size: 959 763
BsmBI CGTCTCn'nnnn_
Cuts at: 0 441 1722
Size: 441 1281
Bsp1286I G_dGCh'C
Cuts at: 0 36 71 100 175 274 322 374 426
Size: 36 35 29 75 99 48 52 52
Cuts at: 426 460 847 1164 1291 1516 1607 1722
Size: 34 387 317 127 225 91 115
Fragments arranged by size:
387 317 225 127 115 99 91 75
52 52 48 36 35 34 29
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 138 1001 1722
Size: 138 863 721
Fragments arranged by size:
863 721 138
BsrBI CCG'CTC
Cuts at: 0 1035 1722
Size: 1035 687
BsrFI r'CCGG_y
Cuts at: 0 20 186 232 614 996 1079 1310 1722
Size: 20 166 46 382 382 83 231 412
Fragments arranged by size:
412 382 382 231 166 83 46 20
BsrGI T'GTAC_A
Cuts at: 0 152 833 1722
Size: 152 681 889
Fragments arranged by size:
889 681 152
BssHII G'CGCG_C
Cuts at: 0 236 694 972 974 1043 1722
Size: 236 458 278 2 69 679
Fragments arranged by size:
679 458 278 236 69 2
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1653 1722
Size: 1653 69
Bsu36I CC'TnA_GG
Cuts at: 0 743 1226 1722
Size: 743 483 496
Fragments arranged by size:
743 496 483
BtgI C'CryG_G
Cuts at: 0 264 620 653 900 1098 1315 1722
Size: 264 356 33 247 198 217 407
Fragments arranged by size:
407 356 264 247 217 198 33
EaeI y'GGCC_r
Cuts at: 0 188 230 336 363 392 650 1095 1205
Size: 188 42 106 27 29 258 445 110
Cuts at: 1205 1312 1415 1722
Size: 107 103 307
Fragments arranged by size:
445 307 258 188 110 107 106 103
42 29 27
EagI C'GGCC_G
Cuts at: 0 188 650 1095 1722
Size: 188 462 445 627
Fragments arranged by size:
627 462 445 188
EarI CTCTTCn'nnn_
Cuts at: 0 716 1722
Size: 716 1006
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 450 920 1722
Size: 450 470 802
Fragments arranged by size:
802 470 450
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1000 1465 1722
Size: 1000 465 257
Fragments arranged by size:
1000 465 257
EcoO109I rG'GnC_Cy
Cuts at: 0 32 33 770 1506 1722
Size: 32 1 737 736 216
Fragments arranged by size:
737 736 216 32 1
FspI TGC'GCA
Cuts at: 0 1003 1722
Size: 1003 719
HaeII r_GCGC'y
Cuts at: 0 50 204 292 547 1482 1722
Size: 50 154 88 255 935 240
Fragments arranged by size:
935 255 240 154 88 50
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 565 597 1722
Size: 565 32 1125
Fragments arranged by size:
1125 565 32
KasI G'GCGC_C
Cuts at: 0 46 288 543 1722
Size: 46 242 255 1179
Fragments arranged by size:
1179 255 242 46
KpnI G_GTAC'C
Cuts at: 0 930 1722
Size: 930 792
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1034 1722
Size: 1034 688
MscI TGG'CCA
Cuts at: 0 365 1207 1722
Size: 365 842 515
Fragments arranged by size:
842 515 365
MslI CAynn'nnrTG
Cuts at: 0 412 1150 1722
Size: 412 738 572
Fragments arranged by size:
738 572 412
MspA1I CmG'CkG
Cuts at: 0 27 113 123 255 622 1100 1340 1722
Size: 27 86 10 132 367 478 240 382
Fragments arranged by size:
478 382 367 240 132 86 27 10
NaeI GCC'GGC
Cuts at: 0 22 188 234 1722
Size: 22 166 46 1488
Fragments arranged by size:
1488 166 46 22
NarI GG'CG_CC
Cuts at: 0 47 289 544 1722
Size: 47 242 255 1178
Fragments arranged by size:
1178 255 242 47
NcoI C'CATG_G
Cuts at: 0 900 1722
Size: 900 822
NgoMIV G'CCGG_C
Cuts at: 0 20 186 232 1722
Size: 20 166 46 1490
Fragments arranged by size:
1490 166 46 20
NsiI A_TGCA'T
Cuts at: 0 1488 1722
Size: 1488 234
NspI r_CATG'y
Cuts at: 0 1490 1722
Size: 1490 232
PasI CC'CwG_GG
Cuts at: 0 1503 1722
Size: 1503 219
PflMI CCAn_nnn'nTGG
Cuts at: 0 1362 1722
Size: 1362 360
PmlI CAC'GTG
Cuts at: 0 1608 1722
Size: 1608 114
PshAI GACnn'nnGTC
Cuts at: 0 878 1722
Size: 878 844
PspOMI G'GGCC_C
Cuts at: 0 32 422 1722
Size: 32 390 1300
Fragments arranged by size:
1300 390 32
PvuII CAG'CTG
Cuts at: 0 27 123 1340 1722
Size: 27 96 1217 382
Fragments arranged by size:
1217 382 96 27
SacII CC_GC'GG
Cuts at: 0 623 1101 1722
Size: 623 478 621
Fragments arranged by size:
623 621 478
SapI GCTCTTCn'nnn_
Cuts at: 0 716 1722
Size: 716 1006
SfoI GGC'GCC
Cuts at: 0 48 290 545 1722
Size: 48 242 255 1177
Fragments arranged by size:
1177 255 242 48
SmaI CCC'GGG
Cuts at: 0 90 220 421 691 1722
Size: 90 130 201 270 1031
Fragments arranged by size:
1031 270 201 130 90
SmlI C'TyrA_G
Cuts at: 0 1474 1597 1722
Size: 1474 123 125
Fragments arranged by size:
1474 125 123
SphI G_CATG'C
Cuts at: 0 1490 1722
Size: 1490 232
SrfI GCCC'GGGC
Cuts at: 0 90 421 1722
Size: 90 331 1301
Fragments arranged by size:
1301 331 90
StuI AGG'CCT
Cuts at: 0 865 1722
Size: 865 857
StyI C'CwwG_G
Cuts at: 0 900 1722
Size: 900 822
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 365 459 1722
Size: 365 94 1263
Fragments arranged by size:
1263 365 94
TatI w'GTAC_w
Cuts at: 0 152 608 833 1169 1722
Size: 152 456 225 336 553
Fragments arranged by size:
553 456 336 225 152
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 442 474 1156 1183 1188 1215 1722
Size: 442 32 682 27 5 27 507
Fragments arranged by size:
682 507 442 32 27 27 5
Tth111I GACn'n_nGTC
Cuts at: 0 1114 1722
Size: 1114 608
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 920 1322 1440 1722
Size: 920 402 118 282
Fragments arranged by size:
920 402 282 118
XmaI C'CCGG_G
Cuts at: 0 88 218 419 689 1722
Size: 88 130 201 270 1033
Fragments arranged by size:
1033 270 201 130 88
Enzymes that do cut:
AarI Acc65I AfeI AhdI AleI AlfI AlwNI ApaI
ApaLI AscI AvaI BanI BanII BbeI BbvCI BfrBI
BglI Bme1580I BmgBI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmBI Bsp1286I BspMI BsrBI BsrFI BsrGI BssHII
BstXI Bsu36I BtgI EaeI EagI EarI EciI Eco57MI
EcoO109I FspI HaeII Hin4I KasI KpnI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PasI PflMI PmlI PshAI PspOMI PvuII SacII SapI
SfoI SmaI SmlI SphI SrfI StuI StyI TaqII
TatI TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AatII AccI AclI AcuI AflII AflIII AgeI AloI
ApoI AseI AsiSI AvrII BaeI BamHI BbsI BcgI
BciVI BclI BglII BlpI BmrI BmtI BsaBI BsaWI
BsmI BspEI BspHI BsrDI BssSI BstAPI BstBI BstEII
BstYI BstZ17I BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspAI
HincII HindIII HpaI MfeI MluI NdeI NheI NotI
NruI PacI PciI PfoI PmeI PpiI PpuMI PsiI
PspXI PsrI PstI PvuI RsrII SacI SalI SanDI
SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SnaBI
SpeI SspI SwaI XbaI XhoI XmnI ZraI