Restriction map for
pF1KE4539
(Linear) (Six-base) MAPSORT of: KIEE4539.seq Check: 9501 from: 1 to: 1866
KIEE4539.orf
With 182 enzymes: SgfI *
April 14, 2015 09:47 ..
AatII G_ACGT'C
Cuts at: 0 1052 1171 1424 1866
Size: 1052 119 253 442
Fragments arranged by size:
1052 442 253 119
AccI GT'mk_AC
Cuts at: 0 712 1866
Size: 712 1154
Acc65I G'GTAC_C
Cuts at: 0 1316 1866
Size: 1316 550
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 60 893 1204 1669 1866
Size: 60 833 311 465 197
Fragments arranged by size:
833 465 311 197 60
AflIII A'CryG_T
Cuts at: 0 1397 1399 1866
Size: 1397 2 467
Fragments arranged by size:
1397 467 2
AhdI GACnn_n'nnGTC
Cuts at: 0 902 1866
Size: 902 964
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 149 1866
Size: 149 1717
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 845 877 1866
Size: 845 32 989
Fragments arranged by size:
989 845 32
AlwNI CAG_nnn'CTG
Cuts at: 0 483 1113 1370 1392 1866
Size: 483 630 257 22 474
Fragments arranged by size:
630 483 474 257 22
ApaI G_GGCC'C
Cuts at: 0 401 1509 1866
Size: 401 1108 357
Fragments arranged by size:
1108 401 357
AvaI C'yCGr_G
Cuts at: 0 99 334 393 456 469 633 827 925
Size: 99 235 59 63 13 164 194 98
Cuts at: 925 1093 1336 1791 1866
Size: 168 243 455 75
Fragments arranged by size:
455 243 235 194 168 164 99 98
75 63 59 13
AvrII C'CTAG_G
Cuts at: 0 1809 1866
Size: 1809 57
BamHI G'GATC_C
Cuts at: 0 1685 1866
Size: 1685 181
BanI G'GyrC_C
Cuts at: 0 1316 1664 1866
Size: 1316 348 202
Fragments arranged by size:
1316 348 202
BanII G_rGCy'C
Cuts at: 0 332 401 468 552 1076 1141 1351 1509
Size: 332 69 67 84 524 65 210 158
Cuts at: 1509 1866
Size: 357
Fragments arranged by size:
524 357 332 210 158 84 69 67
65
BbsI GAAGACnn'nnnn_
Cuts at: 0 901 1866
Size: 901 965
BbvCI CC'TCA_GC
Cuts at: 0 1330 1610 1771 1866
Size: 1330 280 161 95
Fragments arranged by size:
1330 280 161 95
BglI GCCn_nnn'nGGC
Cuts at: 0 598 673 994 1866
Size: 598 75 321 872
Fragments arranged by size:
872 598 321 75
BlpI GC'TnA_GC
Cuts at: 0 529 1345 1866
Size: 529 816 521
Fragments arranged by size:
816 529 521
Bme1580I G_kGCm'C
Cuts at: 0 401 1214 1358 1509 1866
Size: 401 813 144 151 357
Fragments arranged by size:
813 401 357 151 144
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 482 1365 1806 1866
Size: 482 883 441 60
Fragments arranged by size:
883 482 441 60
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 134 1195 1866
Size: 134 1061 671
Fragments arranged by size:
1061 671 134
Bpu10I CC'TnA_GC
Cuts at: 0 229 1330 1610 1771 1866
Size: 229 1101 280 161 95
Fragments arranged by size:
1101 280 229 161 95
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1114 1866
Size: 1114 752
BsaI GGTCTCn'nnnn_
Cuts at: 0 527 1171 1866
Size: 527 644 695
Fragments arranged by size:
695 644 527
BsaAI yAC'GTr
Cuts at: 0 1400 1638 1866
Size: 1400 238 228
Fragments arranged by size:
1400 238 228
BsaHI Gr'CG_yC
Cuts at: 0 347 1049 1168 1421 1798 1866
Size: 347 702 119 253 377 68
Fragments arranged by size:
702 377 347 253 119 68
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1309 1339 1866
Size: 1309 30 527
Fragments arranged by size:
1309 527 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 813 1354 1672 1866
Size: 813 541 318 194
Fragments arranged by size:
813 541 318 194
BseYI C'CCAG_C
Cuts at: 0 506 762 1360 1719 1725 1866
Size: 506 256 598 359 6 141
Fragments arranged by size:
598 506 359 256 141 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 393 1636 1866
Size: 112 281 1243 230
Fragments arranged by size:
1243 281 230 112
BsiEI CG_ry'CG
Cuts at: 0 602 871 1248 1866
Size: 602 269 377 618
Fragments arranged by size:
618 602 377 269
BsiHKAI G_wGCw'C
Cuts at: 0 235 1076 1327 1351 1866
Size: 235 841 251 24 515
Fragments arranged by size:
841 515 251 235 24
Bsp1286I G_dGCh'C
Cuts at: 0 235 332 401 468 552 1076 1141 1214
Size: 235 97 69 67 84 524 65 73
Cuts at: 1214 1327 1351 1358 1509 1866
Size: 113 24 7 151 357
Fragments arranged by size:
524 357 235 151 113 97 84 73
69 67 65 24 7
BspHI T'CATG_A
Cuts at: 0 1568 1866
Size: 1568 298
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 371 458 581 1866
Size: 371 87 123 1285
Fragments arranged by size:
1285 371 123 87
BsrBI CCG'CTC
Cuts at: 0 1662 1866
Size: 1662 204
BsrFI r'CCGG_y
Cuts at: 0 317 1866
Size: 317 1549
BstEII G'GTnAC_C
Cuts at: 0 1087 1866
Size: 1087 779
BstXI CCAn_nnnn'nTGG
Cuts at: 0 199 1005 1866
Size: 199 806 861
Fragments arranged by size:
861 806 199
BstYI r'GATC_y
Cuts at: 0 1484 1685 1866
Size: 1484 201 181
Fragments arranged by size:
1484 201 181
Bsu36I CC'TnA_GG
Cuts at: 0 1142 1226 1866
Size: 1142 84 640
Fragments arranged by size:
1142 640 84
BtgI C'CryG_G
Cuts at: 0 871 1511 1866
Size: 871 640 355
Fragments arranged by size:
871 640 355
BtsI GCAGTG_nn'
Cuts at: 0 1840 1866
Size: 1840 26
EaeI y'GGCC_r
Cuts at: 0 155 590 599 803 868 995 1232 1559
Size: 155 435 9 204 65 127 237 327
Cuts at: 1559 1866
Size: 307
Fragments arranged by size:
435 327 307 237 204 155 127 65
9
EagI C'GGCC_G
Cuts at: 0 599 868 1866
Size: 599 269 998
Fragments arranged by size:
998 599 269
EarI CTCTTCn'nnn_
Cuts at: 0 79 1129 1866
Size: 79 1050 737
Fragments arranged by size:
1050 737 79
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1669 1866
Size: 1669 197
EcoICRI GAG'CTC
Cuts at: 0 1074 1349 1866
Size: 1074 275 517
Fragments arranged by size:
1074 517 275
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 60 134 893 1195 1204 1669 1866
Size: 60 74 759 302 9 465 197
Fragments arranged by size:
759 465 302 197 74 60 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 646 1866
Size: 646 1220
EcoO109I rG'GnC_Cy
Cuts at: 0 32 41 104 339 397 398 637 691
Size: 32 9 63 235 58 1 239 54
Cuts at: 691 747 831 1382 1389 1505 1506 1630 1644
Size: 56 84 551 7 116 1 124 14
Cuts at: 1644 1785 1813 1866
Size: 141 28 53
Fragments arranged by size:
551 239 235 141 124 116 84 63
58 56 54 53 32 28 14 9
7 1 1
HaeII r_GCGC'y
Cuts at: 0 585 1531 1866
Size: 585 946 335
Fragments arranged by size:
946 585 335
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 376 408 723 755 888 920 1811 1843
Size: 376 32 315 32 133 32 891 32
Cuts at: 1843 1866
Size: 23
Fragments arranged by size:
891 376 315 133 32 32 32 32
23
HincII GTy'rAC
Cuts at: 0 713 1203 1601 1866
Size: 713 490 398 265
Fragments arranged by size:
713 490 398 265
KpnI G_GTAC'C
Cuts at: 0 1320 1866
Size: 1320 546
MscI TGG'CCA
Cuts at: 0 157 997 1234 1561 1866
Size: 157 840 237 327 305
Fragments arranged by size:
840 327 305 237 157
MslI CAynn'nnrTG
Cuts at: 0 1003 1567 1866
Size: 1003 564 299
Fragments arranged by size:
1003 564 299
MspA1I CmG'CkG
Cuts at: 0 300 480 873 1044 1416 1698 1866
Size: 300 180 393 171 372 282 168
Fragments arranged by size:
393 372 300 282 180 171 168
NspI r_CATG'y
Cuts at: 0 1779 1866
Size: 1779 87
PasI CC'CwG_GG
Cuts at: 0 1312 1866
Size: 1312 554
PflMI CCAn_nnn'nTGG
Cuts at: 0 1113 1392 1866
Size: 1113 279 474
Fragments arranged by size:
1113 474 279
PfoI T'CCnGG_A
Cuts at: 0 245 1866
Size: 245 1621
PmlI CAC'GTG
Cuts at: 0 1400 1638 1866
Size: 1400 238 228
Fragments arranged by size:
1400 238 228
PpuMI rG'GwC_Cy
Cuts at: 0 32 41 104 339 637 691 831 1389
Size: 32 9 63 235 298 54 140 558
Cuts at: 1389 1630 1813 1866
Size: 241 183 53
Fragments arranged by size:
558 298 241 235 183 140 63 54
53 32 9
PspOMI G'GGCC_C
Cuts at: 0 397 1505 1866
Size: 397 1108 361
Fragments arranged by size:
1108 397 361
PspXI vC'TCGA_Gb
Cuts at: 0 99 1866
Size: 99 1767
PstI C_TGCA'G
Cuts at: 0 683 1866
Size: 683 1183
PvuII CAG'CTG
Cuts at: 0 300 1044 1416 1866
Size: 300 744 372 450
Fragments arranged by size:
744 450 372 300
SacI G_AGCT'C
Cuts at: 0 1076 1351 1866
Size: 1076 275 515
Fragments arranged by size:
1076 515 275
SacII CC_GC'GG
Cuts at: 0 874 1866
Size: 874 992
SalI G'TCGA_C
Cuts at: 0 711 1866
Size: 711 1155
SanDI GG'GwC_CC
Cuts at: 0 41 637 691 831 1630 1866
Size: 41 596 54 140 799 236
Fragments arranged by size:
799 596 236 140 54 41
SapI GCTCTTCn'nnn_
Cuts at: 0 79 1866
Size: 79 1787
SexAI A'CCwGG_T
Cuts at: 0 1757 1866
Size: 1757 109
SfcI C'TryA_G
Cuts at: 0 679 1732 1866
Size: 679 1053 134
Fragments arranged by size:
1053 679 134
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 598 994 1866
Size: 598 396 872
Fragments arranged by size:
872 598 396
SmaI CCC'GGG
Cuts at: 0 635 1866
Size: 635 1231
SmlI C'TyrA_G
Cuts at: 0 99 1129 1866
Size: 99 1030 737
Fragments arranged by size:
1030 737 99
SphI G_CATG'C
Cuts at: 0 1779 1866
Size: 1779 87
StuI AGG'CCT
Cuts at: 0 406 560 1866
Size: 406 154 1306
Fragments arranged by size:
1306 406 154
StyI C'CwwG_G
Cuts at: 0 198 263 1408 1433 1809 1866
Size: 198 65 1145 25 376 57
Fragments arranged by size:
1145 376 198 65 57 25
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 78 1015 1489 1866
Size: 78 937 474 377
Fragments arranged by size:
937 474 377 78
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1622 1654 1866
Size: 1622 32 212
Fragments arranged by size:
1622 212 32
Tth111I GACn'n_nGTC
Cuts at: 0 148 717 1866
Size: 148 569 1149
Fragments arranged by size:
1149 569 148
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1755 1866
Size: 1755 111
XhoI C'TCGA_G
Cuts at: 0 99 1866
Size: 99 1767
XmaI C'CCGG_G
Cuts at: 0 633 1866
Size: 633 1233
ZraI GAC'GTC
Cuts at: 0 1050 1169 1422 1866
Size: 1050 119 253 444
Fragments arranged by size:
1050 444 253 119
Enzymes that do cut:
AatII AccI Acc65I AcuI AflIII AhdI AlfI AloI
AlwNI ApaI AvaI AvrII BamHI BanI BanII BbsI
BbvCI BglI BlpI Bme1580I BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrFI BstEII BstXI
BstYI Bsu36I BtgI BtsI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII KpnI
MscI MslI MspA1I NspI PasI PflMI PfoI PmlI
PpuMI PspOMI PspXI PstI PvuII SacI SacII SalI
SanDI SapI SexAI SfcI SfiI SmaI SmlI SphI
StuI StyI TaqII TstI Tth111I XcmI XhoI XmaI
ZraI
Enzymes that do not cut:
AarI AclI AfeI AflII AgeI AleI ApaLI ApoI
AscI AseI AsiSI BaeI BbeI BcgI BciVI BclI
BfrBI BglII BmgBI BmrI BmtI BsaBI BsaWI BsiWI
BsmI BsmBI BspEI BsrDI BsrGI BssHII BssSI BstAPI
BstBI BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI
EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI
KasI MfeI MluI MmeI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PciI PmeI
PpiI PshAI PsiI PsrI PvuI RsrII SbfI ScaI
SfoI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI
TatI XbaI XmnI