Restriction map for
pF1KE4564
(Linear) (Six-base) MAPSORT of: KIEE4564.seq Check: 6521 from: 1 to: 2106
KIEE4564.orf
With 182 enzymes: SgfI *
January 12, 2017 11:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 804 1338 2106
Size: 804 534 768
Fragments arranged by size:
804 768 534
AccI GT'mk_AC
Cuts at: 0 419 2106
Size: 419 1687
AflIII A'CryG_T
Cuts at: 0 366 2106
Size: 366 1740
AlwNI CAG_nnn'CTG
Cuts at: 0 916 1489 2023 2106
Size: 916 573 534 83
Fragments arranged by size:
916 573 534 83
ApaI G_GGCC'C
Cuts at: 0 278 2106
Size: 278 1828
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 192 225 2106
Size: 192 33 1881
Fragments arranged by size:
1881 192 33
BamHI G'GATC_C
Cuts at: 0 883 2106
Size: 883 1223
BanII G_rGCy'C
Cuts at: 0 278 851 1385 2106
Size: 278 573 534 721
Fragments arranged by size:
721 573 534 278
BbvCI CC'TCA_GC
Cuts at: 0 1297 2106
Size: 1297 809
BciVI GTATCCnnnnn_n'
Cuts at: 0 1886 2106
Size: 1886 220
BfrBI ATG'CAT
Cuts at: 0 313 2106
Size: 313 1793
BglII A'GATC_T
Cuts at: 0 381 2106
Size: 381 1725
Bme1580I G_kGCm'C
Cuts at: 0 278 2106
Size: 278 1828
BmgBI CAC'GTC
Cuts at: 0 369 2106
Size: 369 1737
BmrI ACTGGGnnnn_n'
Cuts at: 0 652 1210 1618 1744 2106
Size: 652 558 408 126 362
Fragments arranged by size:
652 558 408 362 126
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 27 1846 2106
Size: 27 1819 260
Fragments arranged by size:
1819 260 27
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 432 1500 2106
Size: 432 1068 606
Fragments arranged by size:
1068 606 432
Bpu10I CC'TnA_GC
Cuts at: 0 1297 2106
Size: 1297 809
BsaI GGTCTCn'nnnn_
Cuts at: 0 2050 2106
Size: 2050 56
BsaAI yAC'GTr
Cuts at: 0 893 2106
Size: 893 1213
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2072 2102 2106
Size: 2072 30 4
Fragments arranged by size:
2072 30 4
BseYI C'CCAG_C
Cuts at: 0 176 2053 2106
Size: 176 1877 53
Fragments arranged by size:
1877 176 53
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 782 1316 2106
Size: 782 534 790
Fragments arranged by size:
790 782 534
BsiHKAI G_wGCw'C
Cuts at: 0 851 1385 2106
Size: 851 534 721
Fragments arranged by size:
851 721 534
Bsp1286I G_dGCh'C
Cuts at: 0 278 851 1385 2106
Size: 278 573 534 721
Fragments arranged by size:
721 573 534 278
BspHI T'CATG_A
Cuts at: 0 2072 2106
Size: 2072 34
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 804 1338 1435 2106
Size: 804 534 97 671
Fragments arranged by size:
804 671 534 97
BstXI CCAn_nnnn'nTGG
Cuts at: 0 468 1002 1536 2106
Size: 468 534 534 570
Fragments arranged by size:
570 534 534 468
BstYI r'GATC_y
Cuts at: 0 381 883 2106
Size: 381 502 1223
Fragments arranged by size:
1223 502 381
BstZ17I GTA'TAC
Cuts at: 0 420 2106
Size: 420 1686
Bsu36I CC'TnA_GG
Cuts at: 0 1574 2106
Size: 1574 532
BtgI C'CryG_G
Cuts at: 0 467 1001 1535 2106
Size: 467 534 534 571
Fragments arranged by size:
571 534 534 467
BtsI GCAGTG_nn'
Cuts at: 0 95 681 1749 2106
Size: 95 586 1068 357
Fragments arranged by size:
1068 586 357 95
EaeI y'GGCC_r
Cuts at: 0 405 1452 1986 2106
Size: 405 1047 534 120
Fragments arranged by size:
1047 534 405 120
EcoICRI GAG'CTC
Cuts at: 0 849 1383 2106
Size: 849 534 723
Fragments arranged by size:
849 723 534
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 432 1500 2106
Size: 432 1068 606
Fragments arranged by size:
1068 606 432
EcoNI CCTnn'n_nnAGG
Cuts at: 0 45 2106
Size: 45 2061
EcoO109I rG'GnC_Cy
Cuts at: 0 274 593 1127 1165 1661 1952 2106
Size: 274 319 534 38 496 291 154
Fragments arranged by size:
534 496 319 291 274 154 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 20 52 585 617 1119 1151 1653 1685
Size: 20 32 533 32 502 32 502 32
Cuts at: 1685 2106
Size: 421
Fragments arranged by size:
533 502 502 421 32 32 32 32
20
HincII GTy'rAC
Cuts at: 0 1203 2106
Size: 1203 903
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1019 1418 2106
Size: 1019 399 688
Fragments arranged by size:
1019 688 399
MscI TGG'CCA
Cuts at: 0 407 1454 2106
Size: 407 1047 652
Fragments arranged by size:
1047 652 407
MslI CAynn'nnrTG
Cuts at: 0 868 2106
Size: 868 1238
MspA1I CmG'CkG
Cuts at: 0 197 576 1110 1415 1644 2053 2106
Size: 197 379 534 305 229 409 53
Fragments arranged by size:
534 409 379 305 229 197 53
NsiI A_TGCA'T
Cuts at: 0 315 2106
Size: 315 1791
PasI CC'CwG_GG
Cuts at: 0 271 2106
Size: 271 1835
PpuMI rG'GwC_Cy
Cuts at: 0 593 1127 1165 1661 1952 2106
Size: 593 534 38 496 291 154
Fragments arranged by size:
593 534 496 291 154 38
PshAI GACnn'nnGTC
Cuts at: 0 934 1468 2106
Size: 934 534 638
Fragments arranged by size:
934 638 534
PspOMI G'GGCC_C
Cuts at: 0 274 2106
Size: 274 1832
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1042 1074 1576 1608 2106
Size: 1042 32 502 32 498
Fragments arranged by size:
1042 502 498 32 32
PstI C_TGCA'G
Cuts at: 0 575 1109 1643 2106
Size: 575 534 534 463
Fragments arranged by size:
575 534 534 463
PvuII CAG'CTG
Cuts at: 0 197 576 1110 1644 2053 2106
Size: 197 379 534 534 409 53
Fragments arranged by size:
534 534 409 379 197 53
SacI G_AGCT'C
Cuts at: 0 851 1385 2106
Size: 851 534 721
Fragments arranged by size:
851 721 534
ScaI AGT'ACT
Cuts at: 0 805 2106
Size: 805 1301
SfcI C'TryA_G
Cuts at: 0 192 571 1019 1105 1466 1639 2106
Size: 192 379 448 86 361 173 467
Fragments arranged by size:
467 448 379 361 192 173 86
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 856 981 2106
Size: 856 125 1125
Fragments arranged by size:
1125 856 125
TatI w'GTAC_w
Cuts at: 0 803 2106
Size: 803 1303
Tth111I GACn'n_nGTC
Cuts at: 0 1216 1750 2106
Size: 1216 534 356
Fragments arranged by size:
1216 534 356
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 184 2106
Size: 184 1922
Enzymes that do cut:
AarI AccI AflIII AlwNI ApaI BaeI BamHI BanII
BbvCI BciVI BfrBI BglII Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BsaI BsaAI BsaXI BseYI BsgI BsiHKAI
Bsp1286I BspHI BspMI BstXI BstYI BstZ17I Bsu36I BtgI
BtsI EaeI EcoICRI Eco57MI EcoNI EcoO109I Hin4I HincII
MmeI MscI MslI MspA1I NsiI PasI PpuMI PshAI
PspOMI PsrI PstI PvuII SacI ScaI SfcI TaqII
TatI Tth111I XcmI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BanI BbeI BbsI BcgI BclI BglI
BlpI BmtI BpuEI BsaBI BsaHI BsaWI BseRI BsiEI
BsiWI BsmI BsmBI BspEI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BtgZI ClaI CspCI
DraI DraIII DrdI EagI EarI EciI EcoRI EcoRV
FalI FseI FspI FspAI HaeII HindIII HpaI KasI
KpnI MfeI MluI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NspI PacI PciI PflMI PfoI
PmeI PmlI PpiI PsiI PspXI PvuI RsrII SacII
SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI SwaI TstI XbaI XhoI XmaI XmnI
ZraI