(Linear) (Six-base) MAPSORT of: KIEE5061.seq  Check: 6676  from: 1  to: 1173



KIEE5061.orf

 With 182 enzymes: SgfI * 

                        July 16, 2019 14:21  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     712     731    1173 
   Size:        712      19     442

  Fragments arranged by size: 

                 712     442      19
 
AflIII A'CryG_T

Cuts at:      0      29    1173 
   Size:         29    1144
 
ApoI r'AATT_y

Cuts at:      0     154     331     774    1173 
   Size:        154     177     443     399

  Fragments arranged by size: 

                 443     399     177     154
 
AseI AT'TA_AT

Cuts at:      0     374    1173 
   Size:        374     799
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     700    1173 
   Size:        700     473
 
BbvCI CC'TCA_GC

Cuts at:      0     387    1173 
   Size:        387     786
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     996    1173 
   Size:        996     177
 
BclI T'GATC_A

Cuts at:      0      35     596     816    1173 
   Size:         35     561     220     357

  Fragments arranged by size: 

                 561     357     220      35
 
BglII A'GATC_T

Cuts at:      0     542    1173 
   Size:        542     631
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     427     531     844    1173 
   Size:        427     104     313     329

  Fragments arranged by size: 

                 427     329     313     104
 
Bpu10I CC'TnA_GC

Cuts at:      0     387    1173 
   Size:        387     786
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     208     398    1173 
   Size:        208     190     775

  Fragments arranged by size: 

                 775     208     190
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     292     712     844    1045    1173 
   Size:        292     420     132     201     128

  Fragments arranged by size: 

                 420     292     201     132     128
 
BsaBI GATnn'nnATC

Cuts at:      0     742    1173 
   Size:        742     431
 
BsaWI w'CCGG_w

Cuts at:      0     929    1173 
   Size:        929     244
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     160     190     690     720    1173 
   Size:        160      30     500      30     453

  Fragments arranged by size: 

                 500     453     160      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     964    1173 
   Size:        964     209
 
BseYI C'CCAG_C

Cuts at:      0     763    1173 
   Size:        763     410
 
BsmI GAATG_Cn'

Cuts at:      0       9     102    1173 
   Size:          9      93    1071

  Fragments arranged by size: 

                1071      93       9
 
BspEI T'CCGG_A

Cuts at:      0     929    1173 
   Size:        929     244
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     928    1173 
   Size:        928     245
 
BsrDI GCAATG_nn'

Cuts at:      0     168    1173 
   Size:        168    1005
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     729    1173 
   Size:        729     444
 
BstYI r'GATC_y

Cuts at:      0     542    1173 
   Size:        542     631
 
BtsI GCAGTG_nn'

Cuts at:      0     461     599    1173 
   Size:        461     138     574

  Fragments arranged by size: 

                 574     461     138
 
EarI CTCTTCn'nnn_

Cuts at:      0     646    1102    1173 
   Size:        646     456      71

  Fragments arranged by size: 

                 646     456      71
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     427     531     712     731     844    1173 
   Size:        427     104     181      19     113     329

  Fragments arranged by size: 

                 427     329     181     113     104      19
 
EcoRI G'AATT_C

Cuts at:      0     331    1173 
   Size:        331     842
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     160     192     491     523    1173 
   Size:        160      32     299      32     650

  Fragments arranged by size: 

                 650     299     160      32      32
 
HincII GTy'rAC

Cuts at:      0      47     759    1173 
   Size:         47     712     414

  Fragments arranged by size: 

                 712     414      47
 
HindIII A'AGCT_T

Cuts at:      0     616     685    1173 
   Size:        616      69     488

  Fragments arranged by size: 

                 616     488      69
 
MslI CAynn'nnrTG

Cuts at:      0     677    1168    1173 
   Size:        677     491       5

  Fragments arranged by size: 

                 677     491       5
 
MspA1I CmG'CkG

Cuts at:      0     847    1173 
   Size:        847     326
 
PfoI T'CCnGG_A

Cuts at:      0     326    1173 
   Size:        326     847
 
PshAI GACnn'nnGTC

Cuts at:      0     700    1173 
   Size:        700     473
 
PsiI TTA'TAA

Cuts at:      0     505    1079    1173 
   Size:        505     574      94

  Fragments arranged by size: 

                 574     505      94
 
SapI GCTCTTCn'nnn_

Cuts at:      0     646    1173 
   Size:        646     527
 
SmlI C'TyrA_G

Cuts at:      0     223     413    1173 
   Size:        223     190     760

  Fragments arranged by size: 

                 760     223     190
 
SspI AAT'ATT

Cuts at:      0     372    1173 
   Size:        372     801
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     690     722    1173 
   Size:        690      32     451

  Fragments arranged by size: 

                 690     451      32
 

 Enzymes that do cut:

     AcuI   AflIII     ApoI     AseI     BbsI    BbvCI    BciVI     BclI
    BglII     BpmI   Bpu10I    BpuEI     BsaI    BsaBI    BsaWI    BsaXI
    BseRI    BseYI     BsmI    BspEI    BspMI    BsrDI    BstXI    BstYI
     BtsI     EarI  Eco57MI    EcoRI    Hin4I   HincII  HindIII     MslI
   MspA1I     PfoI    PshAI     PsiI     SapI     SmlI     SspI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BcgI    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI    BsaAI    BsaHI     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI
 Bsp1286I    BspHI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRV     FalI     FseI     FspI    FspAI    HaeII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     StuI     StyI     SwaI    TaqII     TatI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI