(Linear) (Six-base) MAPSORT of: KIEE5117.seq  Check: 6901  from: 1  to: 354



KIEE5117.orf

 With 182 enzymes: SgfI * 

                        February 27, 2017 10:28  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     312     354 
   Size:        312      42
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      92     354 
   Size:         92     262
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     153     175     354 
   Size:        153      22     179

  Fragments arranged by size: 

                 179     153      22
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     151     354 
   Size:        151     203
 
BclI T'GATC_A

Cuts at:      0     237     354 
   Size:        237     117
 
Bme1580I G_kGCm'C

Cuts at:      0      50     354 
   Size:         50     304
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     336     354 
   Size:        336      18
 
BsaBI GATnn'nnATC

Cuts at:      0      91     354 
   Size:         91     263
 
BseYI C'CCAG_C

Cuts at:      0      82     354 
   Size:         82     272
 
Bsp1286I G_dGCh'C

Cuts at:      0      50     354 
   Size:         50     304
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     312     354 
   Size:        312      42
 
BstEII G'GTnAC_C

Cuts at:      0      31     354 
   Size:         31     323
 
BtgI C'CryG_G

Cuts at:      0      65     354 
   Size:         65     289
 
EaeI y'GGCC_r

Cuts at:      0      51     354 
   Size:         51     303
 
EarI CTCTTCn'nnn_

Cuts at:      0      68     266     354 
   Size:         68     198      88

  Fragments arranged by size: 

                 198      88      68
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      92     354 
   Size:         92     262
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     277     354 
   Size:        277      77
 
MspA1I CmG'CkG

Cuts at:      0      82     354 
   Size:         82     272
 
PstI C_TGCA'G

Cuts at:      0     327     354 
   Size:        327      27
 
SfcI C'TryA_G

Cuts at:      0     323     354 
   Size:        323      31
 

 Enzymes that do cut:

     AarI     AcuI     BbsI    BciVI     BclI Bme1580I     BplI    BsaBI
    BseYI Bsp1286I    BspMI   BstEII     BtgI     EaeI     EarI  Eco57MI
     FalI   MspA1I     PstI     SfcI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI    BbvCI     BcgI    BfrBI     BglI    BglII     BlpI
    BmgBI     BmrI     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaHI    BsaWI    BsaXI    BseRI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI    BstXI    BstYI  BstZ17I   Bsu36I
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI
     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FseI     FspI
    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     MslI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI