(Linear) (Six-base) MAPSORT of: KIEE5139.seq  Check: 3958  from: 1  to: 345



KIEE5139.orf

 With 182 enzymes: SgfI * 

                        October 24, 2019 20:50  ..

BanII G_rGCy'C

Cuts at:      0      23     129     345 
   Size:         23     106     216

  Fragments arranged by size: 

                 216     106      23
 
Bme1580I G_kGCm'C

Cuts at:      0     324     345 
   Size:        324      21
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      26     345 
   Size:         26     319
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     141     345 
   Size:        141     204
 
BseYI C'CCAG_C

Cuts at:      0      88     345 
   Size:         88     257
 
BsiHKAI G_wGCw'C

Cuts at:      0     129     345 
   Size:        129     216
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     298     345 
   Size:        298      47
 
Bsp1286I G_dGCh'C

Cuts at:      0      23     129     324     345 
   Size:         23     106     195      21

  Fragments arranged by size: 

                 195     106      23      21
 
BspHI T'CATG_A

Cuts at:      0     333     345 
   Size:        333      12
 
EarI CTCTTCn'nnn_

Cuts at:      0      11     131     345 
   Size:         11     120     214

  Fragments arranged by size: 

                 214     120      11
 
EcoICRI GAG'CTC

Cuts at:      0     127     345 
   Size:        127     218
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     141     345 
   Size:        141     204
 
EcoRV GAT'ATC

Cuts at:      0     291     345 
   Size:        291      54
 
HindIII A'AGCT_T

Cuts at:      0     188     345 
   Size:        188     157
 
MslI CAynn'nnrTG

Cuts at:      0     332     345 
   Size:        332      13
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     258     290     345 
   Size:        258      32      55

  Fragments arranged by size: 

                 258      55      32
 
SacI G_AGCT'C

Cuts at:      0     129     345 
   Size:        129     216
 
SapI GCTCTTCn'nnn_

Cuts at:      0      11     345 
   Size:         11     334
 
SexAI A'CCwGG_T

Cuts at:      0     296     345 
   Size:        296      49
 

 Enzymes that do cut:

    BanII Bme1580I     BmrI     BpmI    BseYI  BsiHKAI    BsmBI Bsp1286I
    BspHI     EarI  EcoICRI  Eco57MI    EcoRV  HindIII     MslI     PpiI
     SacI     SapI    SexAI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
    BamHI     BanI     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI    BmgBI     BmtI     BplI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
     BsgI    BsiEI    BsiWI     BsmI    BspEI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EciI    EcoNI EcoO109I
    EcoRI     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII    SacII     SalI    SanDI     SbfI     ScaI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI