(Linear) (Six-base) MAPSORT of: KIEE5152.seq  Check: 4473  from: 1  to: 261



KIEE5152.orf

 With 182 enzymes: SgfI * 

                        October 31, 2014 16:16  ..

BanII G_rGCy'C

Cuts at:      0     125     261 
   Size:        125     136
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     144     178     261 
   Size:        144      34      83

  Fragments arranged by size: 

                 144      83      34
 
BlpI GC'TnA_GC

Cuts at:      0     249     261 
   Size:        249      12
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      94     261 
   Size:         94     167
 
BsaWI w'CCGG_w

Cuts at:      0     205     261 
   Size:        205      56
 
BseYI C'CCAG_C

Cuts at:      0     133     261 
   Size:        133     128
 
Bsp1286I G_dGCh'C

Cuts at:      0     125     261 
   Size:        125     136
 
BspHI T'CATG_A

Cuts at:      0     167     261 
   Size:        167      94
 
BstEII G'GTnAC_C

Cuts at:      0       9     208     261 
   Size:          9     199      53

  Fragments arranged by size: 

                 199      53       9
 
BtgI C'CryG_G

Cuts at:      0     149     261 
   Size:        149     112
 
EaeI y'GGCC_r

Cuts at:      0     195     261 
   Size:        195      66
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      94     261 
   Size:         94     167
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     216     248     261 
   Size:        216      32      13

  Fragments arranged by size: 

                 216      32      13
 
MscI TGG'CCA

Cuts at:      0     197     261 
   Size:        197      64
 
MslI CAynn'nnrTG

Cuts at:      0     166     261 
   Size:        166      95
 
NcoI C'CATG_G

Cuts at:      0     149     261 
   Size:        149     112
 
PasI CC'CwG_GG

Cuts at:      0      15     261 
   Size:         15     246
 
PfoI T'CCnGG_A

Cuts at:      0      50     261 
   Size:         50     211
 
StyI C'CwwG_G

Cuts at:      0     149     261 
   Size:        149     112
 

 Enzymes that do cut:

    BanII     BcgI     BlpI     BpmI    BsaWI    BseYI Bsp1286I    BspHI
   BstEII     BtgI     EaeI  Eco57MI    Hin4I     MscI     MslI     NcoI
     PasI     PfoI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
    BamHI     BanI     BbeI     BbsI    BbvCI    BciVI     BclI    BfrBI
     BglI    BglII Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaXI    BseRI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI    BstXI
    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI   MspA1I
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI