Restriction map for
pF1KE5297
(Linear) (Six-base) MAPSORT of: KIEE5297.seq Check: 5286 from: 1 to: 3411
KIEE5297.orf
With 182 enzymes: SgfI *
December 2, 2014 13:35 ..
AccI GT'mk_AC
Cuts at: 0 671 3411
Size: 671 2740
Acc65I G'GTAC_C
Cuts at: 0 2402 3411
Size: 2402 1009
AclI AA'CG_TT
Cuts at: 0 727 3411
Size: 727 2684
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 355 567 701 750 1171 1527 3411
Size: 355 212 134 49 421 356 1884
Fragments arranged by size:
1884 421 356 355 212 134 49
AfeI AGC'GCT
Cuts at: 0 2225 3411
Size: 2225 1186
AflIII A'CryG_T
Cuts at: 0 635 674 1991 2237 2256 3411
Size: 635 39 1317 246 19 1155
Fragments arranged by size:
1317 1155 635 246 39 19
AleI CACnn'nnGTG
Cuts at: 0 634 1525 1996 2509 2843 3411
Size: 634 891 471 513 334 568
Fragments arranged by size:
891 634 568 513 471 334
AlwNI CAG_nnn'CTG
Cuts at: 0 98 429 1149 1509 2859 2913 3411
Size: 98 331 720 360 1350 54 498
Fragments arranged by size:
1350 720 498 360 331 98 54
ApaI G_GGCC'C
Cuts at: 0 3104 3411
Size: 3104 307
ApaLI G'TGCA_C
Cuts at: 0 1651 2634 3411
Size: 1651 983 777
Fragments arranged by size:
1651 983 777
ApoI r'AATT_y
Cuts at: 0 466 2575 2590 3411
Size: 466 2109 15 821
Fragments arranged by size:
2109 821 466 15
AseI AT'TA_AT
Cuts at: 0 980 3411
Size: 980 2431
AvaI C'yCGr_G
Cuts at: 0 1958 2285 2714 2829 3411
Size: 1958 327 429 115 582
Fragments arranged by size:
1958 582 429 327 115
BamHI G'GATC_C
Cuts at: 0 1034 3411
Size: 1034 2377
BanI G'GyrC_C
Cuts at: 0 178 550 1216 1629 2281 2402 3331 3411
Size: 178 372 666 413 652 121 929 80
Fragments arranged by size:
929 666 652 413 372 178 121 80
BanII G_rGCy'C
Cuts at: 0 1597 2323 2950 3104 3411
Size: 1597 726 627 154 307
Fragments arranged by size:
1597 726 627 307 154
BbeI G_GCGC'C
Cuts at: 0 554 1220 1633 3335 3411
Size: 554 666 413 1702 76
Fragments arranged by size:
1702 666 554 413 76
BbsI GAAGACnn'nnnn_
Cuts at: 0 151 690 1533 1796 2433 2976 3411
Size: 151 539 843 263 637 543 435
Fragments arranged by size:
843 637 543 539 435 263 151
BbvCI CC'TCA_GC
Cuts at: 0 913 1489 3345 3411
Size: 913 576 1856 66
Fragments arranged by size:
1856 913 576 66
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2295 2329 3411
Size: 2295 34 1082
Fragments arranged by size:
2295 1082 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1651 3411
Size: 1651 1760
BclI T'GATC_A
Cuts at: 0 509 1820 3411
Size: 509 1311 1591
Fragments arranged by size:
1591 1311 509
BglI GCCn_nnn'nGGC
Cuts at: 0 448 3411
Size: 448 2963
BglII A'GATC_T
Cuts at: 0 1364 3411
Size: 1364 2047
BlpI GC'TnA_GC
Cuts at: 0 1417 2095 2770 3411
Size: 1417 678 675 641
Fragments arranged by size:
1417 678 675 641
Bme1580I G_kGCm'C
Cuts at: 0 1655 2284 2638 3104 3411
Size: 1655 629 354 466 307
Fragments arranged by size:
1655 629 466 354 307
BmgBI CAC'GTC
Cuts at: 0 891 1994 3411
Size: 891 1103 1417
Fragments arranged by size:
1417 1103 891
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2349 3411
Size: 2349 1062
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 72 94 586 2167 2566 2950 3411
Size: 72 22 492 1581 399 384 461
Fragments arranged by size:
1581 492 461 399 384 72 22
Bpu10I CC'TnA_GC
Cuts at: 0 913 1489 3345 3411
Size: 913 576 1856 66
Fragments arranged by size:
1856 913 576 66
BsaI GGTCTCn'nnnn_
Cuts at: 0 713 2337 3411
Size: 713 1624 1074
Fragments arranged by size:
1624 1074 713
BsaAI yAC'GTr
Cuts at: 0 1413 2259 3411
Size: 1413 846 1152
Fragments arranged by size:
1413 1152 846
BsaHI Gr'CG_yC
Cuts at: 0 551 1217 1630 2558 3332 3411
Size: 551 666 413 928 774 79
Fragments arranged by size:
928 774 666 551 413 79
BsaWI w'CCGG_w
Cuts at: 0 2087 3248 3411
Size: 2087 1161 163
Fragments arranged by size:
2087 1161 163
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 70 100 1678 1708 2164 2194 3411
Size: 70 30 1578 30 456 30 1217
Fragments arranged by size:
1578 1217 456 70 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 358 460 1102 1477 2173 2260 3139 3142
Size: 358 102 642 375 696 87 879 3
Cuts at: 3142 3322 3411
Size: 180 89
Fragments arranged by size:
879 696 642 375 358 180 102 89
87 3
BseYI C'CCAG_C
Cuts at: 0 82 546 2453 2762 2852 3411
Size: 82 464 1907 309 90 559
Fragments arranged by size:
1907 559 464 309 90 82
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 640 1393 2634 2867 3145 3411
Size: 160 480 753 1241 233 278 266
Fragments arranged by size:
1241 753 480 278 266 233 160
BsiHKAI G_wGCw'C
Cuts at: 0 1418 1655 2638 3411
Size: 1418 237 983 773
Fragments arranged by size:
1418 983 773 237
BsmI GAATG_Cn'
Cuts at: 0 696 1574 3318 3411
Size: 696 878 1744 93
Fragments arranged by size:
1744 878 696 93
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2713 3411
Size: 2713 698
Bsp1286I G_dGCh'C
Cuts at: 0 1418 1597 1655 2284 2323 2638 2950 3104
Size: 1418 179 58 629 39 315 312 154
Cuts at: 3104 3411
Size: 307
Fragments arranged by size:
1418 629 315 312 307 179 154 58
39
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 599 1415 3411
Size: 599 816 1996
Fragments arranged by size:
1996 816 599
BsrBI CCG'CTC
Cuts at: 0 896 3411
Size: 896 2515
BsrDI GCAATG_nn'
Cuts at: 0 2540 3411
Size: 2540 871
BsrFI r'CCGG_y
Cuts at: 0 594 1236 2044 3063 3272 3411
Size: 594 642 808 1019 209 139
Fragments arranged by size:
1019 808 642 594 209 139
BsrGI T'GTAC_A
Cuts at: 0 638 1718 3411
Size: 638 1080 1693
Fragments arranged by size:
1693 1080 638
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2019 3411
Size: 2019 1392
BstEII G'GTnAC_C
Cuts at: 0 2517 3411
Size: 2517 894
BstXI CCAn_nnnn'nTGG
Cuts at: 0 951 3411
Size: 951 2460
BstYI r'GATC_y
Cuts at: 0 770 1034 1145 1364 1767 2054 3411
Size: 770 264 111 219 403 287 1357
Fragments arranged by size:
1357 770 403 287 264 219 111
Bsu36I CC'TnA_GG
Cuts at: 0 2357 3411
Size: 2357 1054
BtgI C'CryG_G
Cuts at: 0 122 834 1220 1427 1569 1965 2204 2486
Size: 122 712 386 207 142 396 239 282
Cuts at: 2486 2522 2552 3411
Size: 36 30 859
Fragments arranged by size:
859 712 396 386 282 239 207 142
122 36 30
BtsI GCAGTG_nn'
Cuts at: 0 756 1513 2617 3411
Size: 756 757 1104 794
Fragments arranged by size:
1104 794 757 756
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 104 139 3411
Size: 104 35 3272
Fragments arranged by size:
3272 104 35
DraI TTT'AAA
Cuts at: 0 662 1029 3411
Size: 662 367 2382
Fragments arranged by size:
2382 662 367
DraIII CAC_nnn'GTG
Cuts at: 0 2262 3411
Size: 2262 1149
DrdI GACnn_nn'nnGTC
Cuts at: 0 1270 2671 3411
Size: 1270 1401 740
Fragments arranged by size:
1401 1270 740
EaeI y'GGCC_r
Cuts at: 0 1258 1529 2042 3065 3411
Size: 1258 271 513 1023 346
Fragments arranged by size:
1258 1023 513 346 271
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1063 1393 3259 3411
Size: 1063 330 1866 152
Fragments arranged by size:
1866 1063 330 152
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 72 94 355 567 586 701 750 1171
Size: 72 22 261 212 19 115 49 421
Cuts at: 1171 1527 2167 2566 2950 3411
Size: 356 640 399 384 461
Fragments arranged by size:
640 461 421 399 384 356 261 212
115 72 49 22 19
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1042 3411
Size: 1042 2369
EcoO109I rG'GnC_Cy
Cuts at: 0 258 2136 2711 3015 3101 3411
Size: 258 1878 575 304 86 310
Fragments arranged by size:
1878 575 310 304 258 86
EcoRI G'AATT_C
Cuts at: 0 466 2575 3411
Size: 466 2109 836
Fragments arranged by size:
2109 836 466
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 552 3411
Size: 552 2859
HaeII r_GCGC'y
Cuts at: 0 554 1220 1633 2227 3335 3411
Size: 554 666 413 594 1108 76
Fragments arranged by size:
1108 666 594 554 413 76
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 310 342 1961 1993 3411
Size: 310 32 1619 32 1418
Fragments arranged by size:
1619 1418 310 32 32
HincII GTy'rAC
Cuts at: 0 731 3411
Size: 731 2680
HindIII A'AGCT_T
Cuts at: 0 1642 3411
Size: 1642 1769
KasI G'GCGC_C
Cuts at: 0 550 1216 1629 3331 3411
Size: 550 666 413 1702 80
Fragments arranged by size:
1702 666 550 413 80
KpnI G_GTAC'C
Cuts at: 0 2406 3411
Size: 2406 1005
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2360 3411
Size: 2360 1051
MscI TGG'CCA
Cuts at: 0 1260 1531 3411
Size: 1260 271 1880
Fragments arranged by size:
1880 1260 271
MslI CAynn'nnrTG
Cuts at: 0 634 1525 1996 2491 2509 2779 2843 3411
Size: 634 891 471 495 18 270 64 568
Fragments arranged by size:
891 634 568 495 471 270 64 18
MspA1I CmG'CkG
Cuts at: 0 98 624 836 2397 2453 2509 2554 2766
Size: 98 526 212 1561 56 56 45 212
Cuts at: 2766 3021 3411
Size: 255 390
Fragments arranged by size:
1561 526 390 255 212 212 98 56
56 45
NaeI GCC'GGC
Cuts at: 0 3065 3274 3411
Size: 3065 209 137
Fragments arranged by size:
3065 209 137
NarI GG'CG_CC
Cuts at: 0 551 1217 1630 3332 3411
Size: 551 666 413 1702 79
Fragments arranged by size:
1702 666 551 413 79
NcoI C'CATG_G
Cuts at: 0 1427 1569 2204 2486 3411
Size: 1427 142 635 282 925
Fragments arranged by size:
1427 925 635 282 142
NgoMIV G'CCGG_C
Cuts at: 0 3063 3272 3411
Size: 3063 209 139
Fragments arranged by size:
3063 209 139
NspI r_CATG'y
Cuts at: 0 399 678 1340 2241 3411
Size: 399 279 662 901 1170
Fragments arranged by size:
1170 901 662 399 279
PasI CC'CwG_GG
Cuts at: 0 960 3411
Size: 960 2451
PciI A'CATG_T
Cuts at: 0 674 2237 3411
Size: 674 1563 1174
Fragments arranged by size:
1563 1174 674
PflMI CCAn_nnn'nTGG
Cuts at: 0 429 2859 3411
Size: 429 2430 552
Fragments arranged by size:
2430 552 429
PfoI T'CCnGG_A
Cuts at: 0 563 2168 3071 3411
Size: 563 1605 903 340
Fragments arranged by size:
1605 903 563 340
PmlI CAC'GTG
Cuts at: 0 1413 2259 3411
Size: 1413 846 1152
Fragments arranged by size:
1413 1152 846
PpuMI rG'GwC_Cy
Cuts at: 0 258 2136 2711 3015 3411
Size: 258 1878 575 304 396
Fragments arranged by size:
1878 575 396 304 258
PshAI GACnn'nnGTC
Cuts at: 0 3217 3411
Size: 3217 194
PspOMI G'GGCC_C
Cuts at: 0 3100 3411
Size: 3100 311
PspXI vC'TCGA_Gb
Cuts at: 0 2829 3411
Size: 2829 582
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 3034 3066 3411
Size: 3034 32 345
Fragments arranged by size:
3034 345 32
PstI C_TGCA'G
Cuts at: 0 305 2550 2909 3026 3184 3206 3411
Size: 305 2245 359 117 158 22 205
Fragments arranged by size:
2245 359 305 205 158 117 22
PvuII CAG'CTG
Cuts at: 0 624 2509 2766 3411
Size: 624 1885 257 645
Fragments arranged by size:
1885 645 624 257
RsrII CG'GwC_CG
Cuts at: 0 1050 3411
Size: 1050 2361
SacII CC_GC'GG
Cuts at: 0 837 2555 3411
Size: 837 1718 856
Fragments arranged by size:
1718 856 837
SexAI A'CCwGG_T
Cuts at: 0 2893 3411
Size: 2893 518
SfcI C'TryA_G
Cuts at: 0 301 2546 2905 3022 3180 3202 3411
Size: 301 2245 359 117 158 22 209
Fragments arranged by size:
2245 359 301 209 158 117 22
SfoI GGC'GCC
Cuts at: 0 552 1218 1631 3333 3411
Size: 552 666 413 1702 78
Fragments arranged by size:
1702 666 552 413 78
SgrAI Cr'CCGG_yG
Cuts at: 0 3272 3411
Size: 3272 139
SmaI CCC'GGG
Cuts at: 0 1960 2287 3411
Size: 1960 327 1124
Fragments arranged by size:
1960 1124 327
SmlI C'TyrA_G
Cuts at: 0 2829 3411
Size: 2829 582
SphI G_CATG'C
Cuts at: 0 1340 3411
Size: 1340 2071
SspI AAT'ATT
Cuts at: 0 742 3411
Size: 742 2669
StuI AGG'CCT
Cuts at: 0 1468 2009 2413 3411
Size: 1468 541 404 998
Fragments arranged by size:
1468 998 541 404
StyI C'CwwG_G
Cuts at: 0 866 1427 1569 2204 2486 3411
Size: 866 561 142 635 282 925
Fragments arranged by size:
925 866 635 561 282 142
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 492 1067 1399 1573 2042 3411
Size: 492 575 332 174 469 1369
Fragments arranged by size:
1369 575 492 469 332 174
TatI w'GTAC_w
Cuts at: 0 311 638 1718 3411
Size: 311 327 1080 1693
Fragments arranged by size:
1693 1080 327 311
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2617 2649 3411
Size: 2617 32 762
Fragments arranged by size:
2617 762 32
Tth111I GACn'n_nGTC
Cuts at: 0 1003 3411
Size: 1003 2408
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 216 731 785 3087 3411
Size: 216 515 54 2302 324
Fragments arranged by size:
2302 515 324 216 54
XhoI C'TCGA_G
Cuts at: 0 2829 3411
Size: 2829 582
XmaI C'CCGG_G
Cuts at: 0 1958 2285 3411
Size: 1958 327 1126
Fragments arranged by size:
1958 1126 327
XmnI GAAnn'nnTTC
Cuts at: 0 973 1156 3411
Size: 973 183 2255
Fragments arranged by size:
2255 973 183
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AleI AlwNI
ApaI ApaLI ApoI AseI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BplI BpmI Bpu10I BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BstAPI
BstEII BstXI BstYI Bsu36I BtgI BtsI CspCI DraI
DraIII DrdI EaeI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI HaeII Hin4I HincII HindIII KasI KpnI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PfoI PmlI PpuMI PshAI PspOMI
PspXI PsrI PstI PvuII RsrII SacII SexAI SfcI
SfoI SgrAI SmaI SmlI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AgeI AhdI AlfI AloI AscI
AsiSI AvrII BaeI BfrBI BmrI BmtI BpuEI BsaBI
BsiEI BsiWI BspEI BspHI BssHII BssSI BstBI BstZ17I
BtgZI ClaI EagI EarI EcoICRI EcoRV FseI FspI
FspAI HpaI MfeI MluI NdeI NheI NotI NruI
NsiI PacI PmeI PpiI PsiI PvuI SacI SalI
SanDI SapI SbfI ScaI SfiI SgfI SnaBI SpeI
SrfI SwaI XbaI ZraI