Restriction map for
pF1KE5338
(Linear) (Six-base) MAPSORT of: KIEE5338.seq Check: 2693 from: 1 to: 792
KIEE5338.orf
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AatII G_ACGT'C
Cuts at: 0 443 792
Size: 443 349
Acc65I G'GTAC_C
Cuts at: 0 355 792
Size: 355 437
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 529 792
Size: 529 263
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 370 402 792
Size: 370 32 390
Fragments arranged by size:
390 370 32
AlwNI CAG_nnn'CTG
Cuts at: 0 319 792
Size: 319 473
AvaI C'yCGr_G
Cuts at: 0 623 792
Size: 623 169
BanI G'GyrC_C
Cuts at: 0 10 355 492 792
Size: 10 345 137 300
Fragments arranged by size:
345 300 137 10
BciVI GTATCCnnnnn_n'
Cuts at: 0 621 792
Size: 621 171
Bme1580I G_kGCm'C
Cuts at: 0 13 164 686 792
Size: 13 151 522 106
Fragments arranged by size:
522 151 106 13
BmgBI CAC'GTC
Cuts at: 0 375 792
Size: 375 417
BmrI ACTGGGnnnn_n'
Cuts at: 0 9 471 792
Size: 9 462 321
Fragments arranged by size:
462 321 9
BsaHI Gr'CG_yC
Cuts at: 0 440 792
Size: 440 352
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 3 33 370 400 792
Size: 3 30 337 30 392
Fragments arranged by size:
392 337 30 30 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 31 792
Size: 31 761
BseYI C'CCAG_C
Cuts at: 0 163 208 487 500 762 792
Size: 163 45 279 13 262 30
Fragments arranged by size:
279 262 163 45 30 13
BsiEI CG_ry'CG
Cuts at: 0 702 792
Size: 702 90
BsmI GAATG_Cn'
Cuts at: 0 186 792
Size: 186 606
Bsp1286I G_dGCh'C
Cuts at: 0 13 164 686 792
Size: 13 151 522 106
Fragments arranged by size:
522 151 106 13
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 718 792
Size: 718 74
BsrDI GCAATG_nn'
Cuts at: 0 560 792
Size: 560 232
BstXI CCAn_nnnn'nTGG
Cuts at: 0 670 792
Size: 670 122
BstYI r'GATC_y
Cuts at: 0 204 316 792
Size: 204 112 476
Fragments arranged by size:
476 204 112
BtgI C'CryG_G
Cuts at: 0 669 792
Size: 669 123
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 492 792
Size: 492 300
BtsI GCAGTG_nn'
Cuts at: 0 773 792
Size: 773 19
DraIII CAC_nnn'GTG
Cuts at: 0 375 792
Size: 375 417
EaeI y'GGCC_r
Cuts at: 0 545 792
Size: 545 247
HaeII r_GCGC'y
Cuts at: 0 678 792
Size: 678 114
KpnI G_GTAC'C
Cuts at: 0 359 792
Size: 359 433
MfeI C'AATT_G
Cuts at: 0 331 792
Size: 331 461
MscI TGG'CCA
Cuts at: 0 547 792
Size: 547 245
MslI CAynn'nnrTG
Cuts at: 0 668 792
Size: 668 124
MspA1I CmG'CkG
Cuts at: 0 606 792
Size: 606 186
NcoI C'CATG_G
Cuts at: 0 669 792
Size: 669 123
PflMI CCAn_nnn'nTGG
Cuts at: 0 375 792
Size: 375 417
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 370 402 792
Size: 370 32 390
Fragments arranged by size:
390 370 32
PsiI TTA'TAA
Cuts at: 0 269 792
Size: 269 523
PstI C_TGCA'G
Cuts at: 0 236 729 792
Size: 236 493 63
Fragments arranged by size:
493 236 63
PvuI CG_AT'CG
Cuts at: 0 702 792
Size: 702 90
PvuII CAG'CTG
Cuts at: 0 606 792
Size: 606 186
SfcI C'TryA_G
Cuts at: 0 232 725 792
Size: 232 493 67
Fragments arranged by size:
493 232 67
SmaI CCC'GGG
Cuts at: 0 625 792
Size: 625 167
SspI AAT'ATT
Cuts at: 0 300 792
Size: 300 492
StyI C'CwwG_G
Cuts at: 0 566 669 792
Size: 566 103 123
Fragments arranged by size:
566 123 103
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 28 682 792
Size: 28 654 110
Fragments arranged by size:
654 110 28
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3 35 308 340 792
Size: 3 32 273 32 452
Fragments arranged by size:
452 273 32 32 3
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 484 792
Size: 484 308
XmaI C'CCGG_G
Cuts at: 0 623 792
Size: 623 169
ZraI GAC'GTC
Cuts at: 0 441 792
Size: 441 351
Enzymes that do cut:
AatII Acc65I AlfI AloI AlwNI AvaI BanI BciVI
Bme1580I BmgBI BmrI BsaHI BsaXI BseRI BseYI BsiEI
BsmI Bsp1286I BspMI BsrDI BstXI BstYI BtgI BtgZI
BtsI DraIII EaeI HaeII KpnI MfeI MscI MslI
MspA1I NcoI PflMI PpiI PsiI PstI PvuI PvuII
SfcI SmaI SspI StyI TaqII TstI XcmI XmaI
ZraI
Enzymes that do not cut:
AarI AccI AclI AcuI AfeI AflII AflIII AgeI
AhdI AleI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BanII BbeI BbsI BbvCI BcgI
BclI BfrBI BglI BglII BlpI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BsgI BsiHKAI
BsiWI BsmBI BspEI BspHI BsrBI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstZ17I Bsu36I ClaI CspCI
DraI DrdI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FseI FspI FspAI Hin4I
HincII HindIII HpaI KasI MluI MmeI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PfoI PmeI PmlI PpuMI PshAI PspOMI
PspXI PsrI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmlI
SnaBI SpeI SphI SrfI StuI SwaI TatI Tth111I
XbaI XhoI XmnI