Restriction map for
pF1KE5408
(Linear) (Six-base) MAPSORT of: KIEE5408.seq Check: 8539 from: 1 to: 1008
KIEE5408.orf
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 519 1008
Size: 519 489
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 1008
Size: 196 812
AflIII A'CryG_T
Cuts at: 0 748 1008
Size: 748 260
AhdI GACnn_n'nnGTC
Cuts at: 0 894 1008
Size: 894 114
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 487 1008
Size: 487 521
AlwNI CAG_nnn'CTG
Cuts at: 0 12 165 222 234 1008
Size: 12 153 57 12 774
Fragments arranged by size:
774 153 57 12 12
ApaLI G'TGCA_C
Cuts at: 0 472 871 1008
Size: 472 399 137
Fragments arranged by size:
472 399 137
ApoI r'AATT_y
Cuts at: 0 718 823 1008
Size: 718 105 185
Fragments arranged by size:
718 185 105
AvaI C'yCGr_G
Cuts at: 0 288 1008
Size: 288 720
BanI G'GyrC_C
Cuts at: 0 47 1008
Size: 47 961
BanII G_rGCy'C
Cuts at: 0 731 1008
Size: 731 277
BbsI GAAGACnn'nnnn_
Cuts at: 0 96 111 597 1008
Size: 96 15 486 411
Fragments arranged by size:
486 411 96 15
BlpI GC'TnA_GC
Cuts at: 0 277 1008
Size: 277 731
Bme1580I G_kGCm'C
Cuts at: 0 374 476 875 1008
Size: 374 102 399 133
Fragments arranged by size:
399 374 133 102
BmgBI CAC'GTC
Cuts at: 0 477 1008
Size: 477 531
BmrI ACTGGGnnnn_n'
Cuts at: 0 312 966 1008
Size: 312 654 42
Fragments arranged by size:
654 312 42
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 268 637 1008
Size: 244 24 369 371
Fragments arranged by size:
371 369 244 24
Bpu10I CC'TnA_GC
Cuts at: 0 667 1008
Size: 667 341
BsaAI yAC'GTr
Cuts at: 0 749 1008
Size: 749 259
BsaBI GATnn'nnATC
Cuts at: 0 742 1008
Size: 742 266
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 187 217 298 328 1008
Size: 187 30 81 30 680
Fragments arranged by size:
680 187 81 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 114 208 894 1008
Size: 114 94 686 114
Fragments arranged by size:
686 114 114 94
BseYI C'CCAG_C
Cuts at: 0 81 512 1008
Size: 81 431 496
Fragments arranged by size:
496 431 81
BsiEI CG_ry'CG
Cuts at: 0 599 1008
Size: 599 409
BsiHKAI G_wGCw'C
Cuts at: 0 200 278 344 476 557 673 731 875
Size: 200 78 66 132 81 116 58 144
Cuts at: 875 1008
Size: 133
Fragments arranged by size:
200 144 133 132 116 81 78 66
58
BsmI GAATG_Cn'
Cuts at: 0 422 1008
Size: 422 586
Bsp1286I G_dGCh'C
Cuts at: 0 200 278 344 374 476 557 673 731
Size: 200 78 66 30 102 81 116 58
Cuts at: 731 875 1008
Size: 144 133
Fragments arranged by size:
200 144 133 116 102 81 78 66
58 30
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 158 519 764 1008
Size: 158 361 245 244
Fragments arranged by size:
361 245 244 158
BsrBI CCG'CTC
Cuts at: 0 45 53 1008
Size: 45 8 955
Fragments arranged by size:
955 45 8
BsrFI r'CCGG_y
Cuts at: 0 16 489 1008
Size: 16 473 519
Fragments arranged by size:
519 473 16
BssSI C'ACGA_G
Cuts at: 0 24 199 1008
Size: 24 175 809
Fragments arranged by size:
809 175 24
BstAPI GCAn_nnn'nTGC
Cuts at: 0 26 1008
Size: 26 982
BstXI CCAn_nnnn'nTGG
Cuts at: 0 531 681 925 1008
Size: 531 150 244 83
Fragments arranged by size:
531 244 150 83
BtsI GCAGTG_nn'
Cuts at: 0 119 1008
Size: 119 889
DraIII CAC_nnn'GTG
Cuts at: 0 369 1008
Size: 369 639
EaeI y'GGCC_r
Cuts at: 0 14 360 536 596 965 1008
Size: 14 346 176 60 369 43
Fragments arranged by size:
369 346 176 60 43 14
EagI C'GGCC_G
Cuts at: 0 596 1008
Size: 596 412
EarI CTCTTCn'nnn_
Cuts at: 0 328 1008
Size: 328 680
EcoICRI GAG'CTC
Cuts at: 0 729 1008
Size: 729 279
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 244 268 637 1008
Size: 196 48 24 369 371
Fragments arranged by size:
371 369 196 48 24
EcoNI CCTnn'n_nnAGG
Cuts at: 0 701 1008
Size: 701 307
EcoO109I rG'GnC_Cy
Cuts at: 0 147 1008
Size: 147 861
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 201 1008
Size: 201 807
FspI TGC'GCA
Cuts at: 0 169 1008
Size: 169 839
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 193 225 298 330 880 912 1008
Size: 193 32 73 32 550 32 96
Fragments arranged by size:
550 193 96 73 32 32 32
MscI TGG'CCA
Cuts at: 0 362 538 967 1008
Size: 362 176 429 41
Fragments arranged by size:
429 362 176 41
MslI CAynn'nnrTG
Cuts at: 0 469 1008
Size: 469 539
MspA1I CmG'CkG
Cuts at: 0 222 512 601 925 1008
Size: 222 290 89 324 83
Fragments arranged by size:
324 290 222 89 83
NaeI GCC'GGC
Cuts at: 0 18 1008
Size: 18 990
NgoMIV G'CCGG_C
Cuts at: 0 16 1008
Size: 16 992
PfoI T'CCnGG_A
Cuts at: 0 614 635 1008
Size: 614 21 373
Fragments arranged by size:
614 373 21
PmlI CAC'GTG
Cuts at: 0 749 1008
Size: 749 259
PpuMI rG'GwC_Cy
Cuts at: 0 147 1008
Size: 147 861
PstI C_TGCA'G
Cuts at: 0 8 1008
Size: 8 1000
PvuII CAG'CTG
Cuts at: 0 222 925 1008
Size: 222 703 83
Fragments arranged by size:
703 222 83
SacI G_AGCT'C
Cuts at: 0 731 1008
Size: 731 277
SapI GCTCTTCn'nnn_
Cuts at: 0 328 1008
Size: 328 680
SexAI A'CCwGG_T
Cuts at: 0 98 1008
Size: 98 910
SfcI C'TryA_G
Cuts at: 0 4 1008
Size: 4 1004
StuI AGG'CCT
Cuts at: 0 394 1008
Size: 394 614
StyI C'CwwG_G
Cuts at: 0 558 1008
Size: 558 450
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 985 1008
Size: 985 23
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 187 219 1008
Size: 187 32 789
Fragments arranged by size:
789 187 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 531 1008
Size: 531 477
XmnI GAAnn'nnTTC
Cuts at: 0 823 1008
Size: 823 185
Enzymes that do cut:
AarI AcuI AflIII AhdI AlfI AlwNI ApaLI ApoI
AvaI BanI BanII BbsI BlpI Bme1580I BmgBI BmrI
BpmI Bpu10I BsaAI BsaBI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrFI BssSI BstAPI
BstXI BtsI DraIII EaeI EagI EarI EcoICRI Eco57MI
EcoNI EcoO109I FalI FspI Hin4I MscI MslI MspA1I
NaeI NgoMIV PfoI PmlI PpuMI PstI PvuII SacI
SapI SexAI SfcI StuI StyI TaqII TstI XcmI
XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AleI
AloI ApaI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BmtI BplI BpuEI BsaI BsaHI BsaWI BsgI BsiWI
BsmBI BspEI BspHI BsrDI BsrGI BssHII BstBI BstEII
BstYI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI
DrdI EciI EcoRI EcoRV FseI FspAI HaeII HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI NarI
NcoI NdeI NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI
ScaI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI SwaI TatI Tth111I XbaI
XhoI XmaI ZraI