(Linear) (Six-base) MAPSORT of: KIEE5454.seq  Check: 1124  from: 1  to: 1059



KIEE5454.orf

 With 182 enzymes: SgfI * 

                        July 16, 2019 14:21  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      21    1059 
   Size:         21    1038
 
AflIII A'CryG_T

Cuts at:      0      99    1059 
   Size:         99     960
 
AleI CACnn'nnGTG

Cuts at:      0     520    1059 
   Size:        520     539
 
ApoI r'AATT_y

Cuts at:      0     599    1059 
   Size:        599     460
 
AvaI C'yCGr_G

Cuts at:      0    1054    1059 
   Size:       1054       5
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     801     834    1059 
   Size:        801      33     225

  Fragments arranged by size: 

                 801     225      33
 
BanI G'GyrC_C

Cuts at:      0     906    1059 
   Size:        906     153
 
BanII G_rGCy'C

Cuts at:      0     563     803    1059 
   Size:        563     240     256

  Fragments arranged by size: 

                 563     256     240
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      27    1059 
   Size:         27    1032
 
BbvCI CC'TCA_GC

Cuts at:      0     155    1046    1059 
   Size:        155     891      13

  Fragments arranged by size: 

                 891     155      13
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1023    1057    1059 
   Size:       1023      34       2

  Fragments arranged by size: 

                1023      34       2
 
BclI T'GATC_A

Cuts at:      0     288    1059 
   Size:        288     771
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     978    1059 
   Size:        978      81
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     999    1059 
   Size:        999      60
 
Bpu10I CC'TnA_GC

Cuts at:      0     155    1046    1059 
   Size:        155     891      13

  Fragments arranged by size: 

                 891     155      13
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      71    1059 
   Size:         71     988
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     547    1059 
   Size:        547     512
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     858    1059 
   Size:        858     201
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     373     511    1059 
   Size:        373     138     548

  Fragments arranged by size: 

                 548     373     138
 
BsiHKAI G_wGCw'C

Cuts at:      0     563    1059 
   Size:        563     496
 
BsmI GAATG_Cn'

Cuts at:      0     166    1059 
   Size:        166     893
 
Bsp1286I G_dGCh'C

Cuts at:      0     563     803    1059 
   Size:        563     240     256

  Fragments arranged by size: 

                 563     256     240
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     621    1059 
   Size:        621     438
 
BsrDI GCAATG_nn'

Cuts at:      0     205     372     992    1059 
   Size:        205     167     620      67

  Fragments arranged by size: 

                 620     205     167      67
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1015    1059 
   Size:       1015      44
 
BtsI GCAGTG_nn'

Cuts at:      0     356    1004    1059 
   Size:        356     648      55

  Fragments arranged by size: 

                 648     356      55
 
DraI TTT'AAA

Cuts at:      0     716    1059 
   Size:        716     343
 
DraIII CAC_nnn'GTG

Cuts at:      0     411    1059 
   Size:        411     648
 
EarI CTCTTCn'nnn_

Cuts at:      0     465    1059 
   Size:        465     594
 
EcoICRI GAG'CTC

Cuts at:      0     561    1059 
   Size:        561     498
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      21     999    1059 
   Size:         21     978      60

  Fragments arranged by size: 

                 978      60      21
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1054    1059 
   Size:       1054       5
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     280     312     986    1018    1059 
   Size:        280      32     674      32      41

  Fragments arranged by size: 

                 674     280      41      32      32
 
HincII GTy'rAC

Cuts at:      0     822    1059 
   Size:        822     237
 
HpaI GTT'AAC

Cuts at:      0     822    1059 
   Size:        822     237
 
MslI CAynn'nnrTG

Cuts at:      0     520    1059 
   Size:        520     539
 
MspA1I CmG'CkG

Cuts at:      0     294     398    1059 
   Size:        294     104     661

  Fragments arranged by size: 

                 661     294     104
 
NspI r_CATG'y

Cuts at:      0     103    1059 
   Size:        103     956
 
PciI A'CATG_T

Cuts at:      0      99    1059 
   Size:         99     960
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     699    1059 
   Size:        699     360
 
PvuII CAG'CTG

Cuts at:      0     294    1059 
   Size:        294     765
 
SacI G_AGCT'C

Cuts at:      0     563    1059 
   Size:        563     496
 
SexAI A'CCwGG_T

Cuts at:      0     407     485    1059 
   Size:        407      78     574

  Fragments arranged by size: 

                 574     407      78
 
SmlI C'TyrA_G

Cuts at:      0      86    1059 
   Size:         86     973
 
SspI AAT'ATT

Cuts at:      0     842    1059 
   Size:        842     217
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     483    1059 
   Size:        483     576
 

 Enzymes that do cut:

     AcuI   AflIII     AleI     ApoI     AvaI     BaeI     BanI    BanII
     BbsI    BbvCI     BcgI     BclI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BseRI     BsgI  BsiHKAI     BsmI Bsp1286I    BspMI    BsrDI
   BstAPI     BtsI     DraI   DraIII     EarI  EcoICRI  Eco57MI     FalI
    Hin4I   HincII     HpaI     MslI   MspA1I     NspI     PciI    PflMI
    PvuII     SacI    SexAI     SmlI     SspI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI    AvrII    BamHI     BbeI    BciVI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseYI    BsiEI    BsiWI    BsmBI    BspEI    BspHI
    BsrBI    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DrdI
     EaeI     EagI     EciI    EcoNI EcoO109I    EcoRI    EcoRV     FseI
     FspI    FspAI    HaeII  HindIII     KasI     KpnI     MfeI     MluI
     MmeI     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     StuI     StyI     SwaI    TaqII     TatI     TstI
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI