Restriction map for
pF1KE5553
(Linear) (Six-base) MAPSORT of: KIEE5553.seq Check: 2381 from: 1 to: 1278
KIEE5553.orf
With 182 enzymes: SgfI *
September 30, 2015 09:49 ..
AccI GT'mk_AC
Cuts at: 0 875 1278
Size: 875 403
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 687 986 1278
Size: 70 617 299 292
Fragments arranged by size:
617 299 292 70
AflII C'TTAA_G
Cuts at: 0 1042 1278
Size: 1042 236
AflIII A'CryG_T
Cuts at: 0 29 1278
Size: 29 1249
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 16 48 55 87 1278
Size: 16 32 7 32 1191
Fragments arranged by size:
1191 32 32 16 7
ApoI r'AATT_y
Cuts at: 0 612 1278
Size: 612 666
BamHI G'GATC_C
Cuts at: 0 752 1278
Size: 752 526
BanII G_rGCy'C
Cuts at: 0 59 68 1196 1278
Size: 59 9 1128 82
Fragments arranged by size:
1128 82 59 9
BbsI GAAGACnn'nnnn_
Cuts at: 0 607 910 1278
Size: 607 303 368
Fragments arranged by size:
607 368 303
BclI T'GATC_A
Cuts at: 0 1115 1278
Size: 1115 163
BglII A'GATC_T
Cuts at: 0 170 1278
Size: 170 1108
Bme1580I G_kGCm'C
Cuts at: 0 779 1278
Size: 779 499
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 1072 1278
Size: 109 963 206
Fragments arranged by size:
963 206 109
BsaAI yAC'GTr
Cuts at: 0 30 116 1278
Size: 30 86 1162
Fragments arranged by size:
1162 86 30
BsaHI Gr'CG_yC
Cuts at: 0 222 1278
Size: 222 1056
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 16 46 55 85 1278
Size: 16 30 9 30 1193
Fragments arranged by size:
1193 30 30 16 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 386 702 1183 1278
Size: 386 316 481 95
Fragments arranged by size:
481 386 316 95
BseYI C'CCAG_C
Cuts at: 0 251 1278
Size: 251 1027
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 756 1278
Size: 756 522
BsiHKAI G_wGCw'C
Cuts at: 0 1196 1278
Size: 1196 82
BsmI GAATG_Cn'
Cuts at: 0 1251 1278
Size: 1251 27
Bsp1286I G_dGCh'C
Cuts at: 0 59 68 779 1196 1278
Size: 59 9 711 417 82
Fragments arranged by size:
711 417 82 59 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 371 1278
Size: 371 907
BsrDI GCAATG_nn'
Cuts at: 0 119 800 1278
Size: 119 681 478
Fragments arranged by size:
681 478 119
BstAPI GCAn_nnn'nTGC
Cuts at: 0 475 1278
Size: 475 803
BstYI r'GATC_y
Cuts at: 0 170 752 1278
Size: 170 582 526
Fragments arranged by size:
582 526 170
BtgI C'CryG_G
Cuts at: 0 20 59 806 866 1278
Size: 20 39 747 60 412
Fragments arranged by size:
747 412 60 39 20
BtsI GCAGTG_nn'
Cuts at: 0 277 1074 1278
Size: 277 797 204
Fragments arranged by size:
797 277 204
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 529 564 1278
Size: 529 35 714
Fragments arranged by size:
714 529 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 109 1278
Size: 109 1169
EaeI y'GGCC_r
Cuts at: 0 2 17 1278
Size: 2 15 1261
Fragments arranged by size:
1261 15 2
EarI CTCTTCn'nnn_
Cuts at: 0 1003 1278
Size: 1003 275
EcoICRI GAG'CTC
Cuts at: 0 1194 1278
Size: 1194 84
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 109 687 986 1072 1278
Size: 70 39 578 299 86 206
Fragments arranged by size:
578 299 206 86 70 39
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1012 1044 1056 1088 1278
Size: 1012 32 12 32 190
Fragments arranged by size:
1012 190 32 32 12
MfeI C'AATT_G
Cuts at: 0 581 1278
Size: 581 697
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 921 1278
Size: 921 357
MscI TGG'CCA
Cuts at: 0 4 19 1278
Size: 4 15 1259
Fragments arranged by size:
1259 15 4
MslI CAynn'nnrTG
Cuts at: 0 124 805 1035 1278
Size: 124 681 230 243
Fragments arranged by size:
681 243 230 124
MspA1I CmG'CkG
Cuts at: 0 372 391 1278
Size: 372 19 887
Fragments arranged by size:
887 372 19
PmlI CAC'GTG
Cuts at: 0 116 1278
Size: 116 1162
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 16 48 55 87 1278
Size: 16 32 7 32 1191
Fragments arranged by size:
1191 32 32 16 7
PstI C_TGCA'G
Cuts at: 0 284 1158 1278
Size: 284 874 120
Fragments arranged by size:
874 284 120
PvuII CAG'CTG
Cuts at: 0 391 1278
Size: 391 887
SacI G_AGCT'C
Cuts at: 0 1196 1278
Size: 1196 82
ScaI AGT'ACT
Cuts at: 0 595 1278
Size: 595 683
SfcI C'TryA_G
Cuts at: 0 280 1154 1278
Size: 280 874 124
Fragments arranged by size:
874 280 124
SmlI C'TyrA_G
Cuts at: 0 1042 1278
Size: 1042 236
SspI AAT'ATT
Cuts at: 0 1059 1278
Size: 1059 219
StyI C'CwwG_G
Cuts at: 0 1107 1197 1278
Size: 1107 90 81
Fragments arranged by size:
1107 90 81
TatI w'GTAC_w
Cuts at: 0 593 635 1278
Size: 593 42 643
Fragments arranged by size:
643 593 42
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 12 1278
Size: 12 1266
Enzymes that do cut:
AccI AcuI AflII AflIII AloI ApoI BamHI BanII
BbsI BclI BglII Bme1580I BpmI BsaAI BsaHI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrDI
BstAPI BstYI BtgI BtsI CspCI DrdI EaeI EarI
EcoICRI Eco57MI Hin4I MfeI MmeI MscI MslI MspA1I
PmlI PpiI PstI PvuII SacI ScaI SfcI SmlI
SspI StyI TatI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AhdI AleI
AlfI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BanI BbeI BbvCI BcgI BciVI BfrBI
BglI BlpI BmgBI BmrI BmtI BplI Bpu10I BpuEI
BsaI BsaBI BsaWI BsiEI BsiWI BsmBI BspEI BspHI
BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII BstXI
BstZ17I Bsu36I BtgZI ClaI DraI DraIII EagI EciI
EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI
HaeII HincII HindIII HpaI KasI KpnI MluI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PflMI PfoI PmeI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII
SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI
TaqII TstI Tth111I XbaI XhoI XmaI XmnI ZraI