Restriction map for
pF1KE5572
(Linear) (Six-base) MAPSORT of: KIEE5572.seq Check: 3886 from: 1 to: 1383
KIEE5572.orf
With 182 enzymes: SgfI *
May 19, 2017 16:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 826 1383
Size: 826 557
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 635 1090 1271 1383
Size: 635 455 181 112
Fragments arranged by size:
635 455 181 112
AflIII A'CryG_T
Cuts at: 0 397 1141 1178 1383
Size: 397 744 37 205
Fragments arranged by size:
744 397 205 37
ApoI r'AATT_y
Cuts at: 0 116 256 606 1039 1383
Size: 116 140 350 433 344
Fragments arranged by size:
433 350 344 140 116
AseI AT'TA_AT
Cuts at: 0 335 926 1383
Size: 335 591 457
Fragments arranged by size:
591 457 335
AvaI C'yCGr_G
Cuts at: 0 674 1383
Size: 674 709
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1322 1355 1383
Size: 1322 33 28
Fragments arranged by size:
1322 33 28
BbsI GAAGACnn'nnnn_
Cuts at: 0 1068 1138 1383
Size: 1068 70 245
Fragments arranged by size:
1068 245 70
BciVI GTATCCnnnnn_n'
Cuts at: 0 1350 1383
Size: 1350 33
BclI T'GATC_A
Cuts at: 0 17 96 1383
Size: 17 79 1287
Fragments arranged by size:
1287 79 17
BfrBI ATG'CAT
Cuts at: 0 748 1383
Size: 748 635
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 199 1383
Size: 199 1184
BsaBI GATnn'nnATC
Cuts at: 0 281 1383
Size: 281 1102
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 868 1383
Size: 868 515
BseYI C'CCAG_C
Cuts at: 0 1064 1289 1383
Size: 1064 225 94
Fragments arranged by size:
1064 225 94
BsmI GAATG_Cn'
Cuts at: 0 748 1383
Size: 748 635
BspHI T'CATG_A
Cuts at: 0 99 1383
Size: 99 1284
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 711 826 1383
Size: 711 115 557
Fragments arranged by size:
711 557 115
BsrDI GCAATG_nn'
Cuts at: 0 944 1004 1313 1383
Size: 944 60 309 70
Fragments arranged by size:
944 309 70 60
BsrGI T'GTAC_A
Cuts at: 0 82 1383
Size: 82 1301
BssSI C'ACGA_G
Cuts at: 0 221 1383
Size: 221 1162
BstBI TT'CG_AA
Cuts at: 0 891 1383
Size: 891 492
BstYI r'GATC_y
Cuts at: 0 1045 1383
Size: 1045 338
BtsI GCAGTG_nn'
Cuts at: 0 524 1383
Size: 524 859
DraI TTT'AAA
Cuts at: 0 308 1383
Size: 308 1075
EaeI y'GGCC_r
Cuts at: 0 127 1383
Size: 127 1256
EarI CTCTTCn'nnn_
Cuts at: 0 152 531 1065 1292 1383
Size: 152 379 534 227 91
Fragments arranged by size:
534 379 227 152 91
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 635 1090 1271 1383
Size: 635 455 181 112
Fragments arranged by size:
635 455 181 112
EcoO109I rG'GnC_Cy
Cuts at: 0 1237 1383
Size: 1237 146
EcoRV GAT'ATC
Cuts at: 0 64 481 1383
Size: 64 417 902
Fragments arranged by size:
902 417 64
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 193 1383
Size: 193 1190
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 53 85 749 781 1383
Size: 53 32 664 32 602
Fragments arranged by size:
664 602 53 32 32
HincII GTy'rAC
Cuts at: 0 1131 1353 1383
Size: 1131 222 30
Fragments arranged by size:
1131 222 30
MslI CAynn'nnrTG
Cuts at: 0 19 528 1383
Size: 19 509 855
Fragments arranged by size:
855 509 19
NsiI A_TGCA'T
Cuts at: 0 750 1383
Size: 750 633
NspI r_CATG'y
Cuts at: 0 401 1145 1182 1383
Size: 401 744 37 201
Fragments arranged by size:
744 401 201 37
PciI A'CATG_T
Cuts at: 0 397 1141 1178 1383
Size: 397 744 37 205
Fragments arranged by size:
744 397 205 37
PpuMI rG'GwC_Cy
Cuts at: 0 1237 1383
Size: 1237 146
SanDI GG'GwC_CC
Cuts at: 0 1237 1383
Size: 1237 146
SexAI A'CCwGG_T
Cuts at: 0 702 1383
Size: 702 681
SmlI C'TyrA_G
Cuts at: 0 178 1383
Size: 178 1205
SspI AAT'ATT
Cuts at: 0 849 1383
Size: 849 534
StuI AGG'CCT
Cuts at: 0 38 1383
Size: 38 1345
StyI C'CwwG_G
Cuts at: 0 715 1334 1383
Size: 715 619 49
Fragments arranged by size:
715 619 49
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 667 1148 1383
Size: 667 481 235
Fragments arranged by size:
667 481 235
TatI w'GTAC_w
Cuts at: 0 82 469 932 1383
Size: 82 387 463 451
Fragments arranged by size:
463 451 387 82
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 187 219 1383
Size: 187 32 1164
Fragments arranged by size:
1164 187 32
XmnI GAAnn'nnTTC
Cuts at: 0 540 1030 1383
Size: 540 490 353
Fragments arranged by size:
540 490 353
Enzymes that do cut:
AarI AcuI AflIII ApoI AseI AvaI BaeI BbsI
BciVI BclI BfrBI BpuEI BsaBI BseRI BseYI BsmI
BspHI BspMI BsrDI BsrGI BssSI BstBI BstYI BtsI
DraI EaeI EarI Eco57MI EcoO109I EcoRV FalI Hin4I
HincII MslI NsiI NspI PciI PpuMI SanDI SexAI
SmlI SspI StuI StyI TaqII TatI TstI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI AscI AsiSI
AvrII BamHI BanI BanII BbeI BbvCI BcgI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BsaI BsaAI BsaHI BsaWI BsaXI BsgI BsiEI
BsiHKAI BsiWI BsmBI Bsp1286I BspEI BsrBI BsrFI BssHII
BstAPI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI ClaI
CspCI DraIII DrdI EagI EciI EcoICRI EcoNI EcoRI
FseI FspI FspAI HaeII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI PacI PasI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SwaI Tth111I XbaI
XcmI XhoI XmaI ZraI