Restriction map for
pF1KE5778
(Linear) (Six-base) MAPSORT of: KIEE5778.seq Check: 4788 from: 1 to: 1875
KIEE5778.orf
With 182 enzymes: SgfI *
June 20, 2019 10:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 91 1194 1875
Size: 91 1103 681
Fragments arranged by size:
1103 681 91
AatII G_ACGT'C
Cuts at: 0 431 1875
Size: 431 1444
AccI GT'mk_AC
Cuts at: 0 495 837 1331 1875
Size: 495 342 494 544
Fragments arranged by size:
544 495 494 342
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 26 520 1875
Size: 26 494 1355
Fragments arranged by size:
1355 494 26
AfeI AGC'GCT
Cuts at: 0 324 1875
Size: 324 1551
AleI CACnn'nnGTG
Cuts at: 0 1244 1828 1875
Size: 1244 584 47
Fragments arranged by size:
1244 584 47
AlwNI CAG_nnn'CTG
Cuts at: 0 49 957 1875
Size: 49 908 918
Fragments arranged by size:
918 908 49
ApaLI G'TGCA_C
Cuts at: 0 578 960 1875
Size: 578 382 915
Fragments arranged by size:
915 578 382
BamHI G'GATC_C
Cuts at: 0 204 1875
Size: 204 1671
BbsI GAAGACnn'nnnn_
Cuts at: 0 105 1875
Size: 105 1770
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1478 1512 1777 1811 1875
Size: 1478 34 265 34 64
Fragments arranged by size:
1478 265 64 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1096 1875
Size: 1096 779
BclI T'GATC_A
Cuts at: 0 1407 1875
Size: 1407 468
Bme1580I G_kGCm'C
Cuts at: 0 582 964 1875
Size: 582 382 911
Fragments arranged by size:
911 582 382
BmrI ACTGGGnnnn_n'
Cuts at: 0 788 856 1875
Size: 788 68 1019
Fragments arranged by size:
1019 788 68
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 836 1875
Size: 836 1039
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 496 1579 1875
Size: 496 1083 296
Fragments arranged by size:
1083 496 296
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 383 1850 1875
Size: 383 1467 25
Fragments arranged by size:
1467 383 25
BsaAI yAC'GTr
Cuts at: 0 449 1842 1875
Size: 449 1393 33
Fragments arranged by size:
1393 449 33
BsaHI Gr'CG_yC
Cuts at: 0 428 1875
Size: 428 1447
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1103 1875
Size: 1103 772
BseYI C'CCAG_C
Cuts at: 0 469 999 1541 1781 1875
Size: 469 530 542 240 94
Fragments arranged by size:
542 530 469 240 94
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 132 672 1198 1875
Size: 132 540 526 677
Fragments arranged by size:
677 540 526 132
BsiHKAI G_wGCw'C
Cuts at: 0 399 582 964 1800 1875
Size: 399 183 382 836 75
Fragments arranged by size:
836 399 382 183 75
BsiWI C'GTAC_G
Cuts at: 0 449 1875
Size: 449 1426
BsmI GAATG_Cn'
Cuts at: 0 409 1875
Size: 409 1466
Bsp1286I G_dGCh'C
Cuts at: 0 399 582 964 1800 1875
Size: 399 183 382 836 75
Fragments arranged by size:
836 399 382 183 75
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 91 1194 1875
Size: 91 1103 681
Fragments arranged by size:
1103 681 91
BsrBI CCG'CTC
Cuts at: 0 1288 1809 1875
Size: 1288 521 66
Fragments arranged by size:
1288 521 66
BsrDI GCAATG_nn'
Cuts at: 0 310 1875
Size: 310 1565
BsrFI r'CCGG_y
Cuts at: 0 1681 1875
Size: 1681 194
BssSI C'ACGA_G
Cuts at: 0 940 1776 1875
Size: 940 836 99
Fragments arranged by size:
940 836 99
BstEII G'GTnAC_C
Cuts at: 0 1206 1875
Size: 1206 669
BstYI r'GATC_y
Cuts at: 0 204 1060 1875
Size: 204 856 815
Fragments arranged by size:
856 815 204
BtgI C'CryG_G
Cuts at: 0 1198 1875
Size: 1198 677
BtsI GCAGTG_nn'
Cuts at: 0 253 432 1875
Size: 253 179 1443
Fragments arranged by size:
1443 253 179
DraI TTT'AAA
Cuts at: 0 1346 1875
Size: 1346 529
EaeI y'GGCC_r
Cuts at: 0 678 1195 1810 1875
Size: 678 517 615 65
Fragments arranged by size:
678 615 517 65
EarI CTCTTCn'nnn_
Cuts at: 0 178 1622 1633 1875
Size: 178 1444 11 242
Fragments arranged by size:
1444 242 178 11
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 202 1875
Size: 202 1673
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 26 496 520 1579 1875
Size: 26 470 24 1059 296
Fragments arranged by size:
1059 470 296 26 24
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1508 1875
Size: 1508 367
EcoO109I rG'GnC_Cy
Cuts at: 0 1729 1875
Size: 1729 146
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1056 1629 1875
Size: 1056 573 246
Fragments arranged by size:
1056 573 246
HaeII r_GCGC'y
Cuts at: 0 128 326 1875
Size: 128 198 1549
Fragments arranged by size:
1549 198 128
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 155 187 1079 1111 1241 1273 1357 1389
Size: 155 32 892 32 130 32 84 32
Cuts at: 1389 1875
Size: 486
Fragments arranged by size:
892 486 155 130 84 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 1045 1875
Size: 1045 830
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1090 1146 1875
Size: 1090 56 729
Fragments arranged by size:
1090 729 56
MslI CAynn'nnrTG
Cuts at: 0 343 862 1244 1828 1875
Size: 343 519 382 584 47
Fragments arranged by size:
584 519 382 343 47
MspA1I CmG'CkG
Cuts at: 0 977 1875
Size: 977 898
NaeI GCC'GGC
Cuts at: 0 1683 1875
Size: 1683 192
NdeI CA'TA_TG
Cuts at: 0 1495 1875
Size: 1495 380
NgoMIV G'CCGG_C
Cuts at: 0 1681 1875
Size: 1681 194
PflMI CCAn_nnn'nTGG
Cuts at: 0 215 1875
Size: 215 1660
PstI C_TGCA'G
Cuts at: 0 850 1875
Size: 850 1025
ScaI AGT'ACT
Cuts at: 0 133 1875
Size: 133 1742
SfcI C'TryA_G
Cuts at: 0 846 1332 1686 1875
Size: 846 486 354 189
Fragments arranged by size:
846 486 354 189
SmlI C'TyrA_G
Cuts at: 0 398 1865 1875
Size: 398 1467 10
Fragments arranged by size:
1467 398 10
StuI AGG'CCT
Cuts at: 0 946 1875
Size: 946 929
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 173 523 1242 1533 1875
Size: 173 350 719 291 342
Fragments arranged by size:
719 350 342 291 173
TatI w'GTAC_w
Cuts at: 0 131 796 1875
Size: 131 665 1079
Fragments arranged by size:
1079 665 131
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 681 713 1120 1152 1875
Size: 681 32 407 32 723
Fragments arranged by size:
723 681 407 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 649 1875
Size: 649 1226
ZraI GAC'GTC
Cuts at: 0 429 1875
Size: 429 1446
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AleI AlwNI ApaLI
BamHI BbsI BcgI BciVI BclI Bme1580I BmrI BplI
BpmI BpuEI BsaAI BsaHI BseRI BseYI BsgI BsiHKAI
BsiWI BsmI Bsp1286I BspMI BsrBI BsrDI BsrFI BssSI
BstEII BstYI BtgI BtsI DraI EaeI EarI EciI
Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HindIII MmeI
MslI MspA1I NaeI NdeI NgoMIV PflMI PstI ScaI
SfcI SmlI StuI TaqII TatI TstI Tth111I ZraI
Enzymes that do not cut:
Acc65I AclI AflII AflIII AgeI AhdI AlfI AloI
ApaI ApoI AscI AseI AsiSI AvaI AvrII BaeI
BanI BanII BbeI BbvCI BfrBI BglI BglII BlpI
BmgBI BmtI Bpu10I BsaI BsaBI BsaWI BsaXI BsiEI
BsmBI BspEI BspHI BsrGI BssHII BstAPI BstBI BstXI
BstZ17I Bsu36I BtgZI ClaI CspCI DraIII DrdI EagI
EcoICRI EcoRI EcoRV FseI FspI FspAI HincII HpaI
KasI KpnI MfeI MluI MscI NarI NcoI NheI
NotI NruI NsiI NspI PacI PasI PciI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StyI SwaI XbaI
XcmI XhoI XmaI XmnI