Restriction map for
pF1KE5841
(Linear) (Six-base) MAPSORT of: KIEE5841.seq Check: 6674 from: 1 to: 5394
KIEE5841.orf
With 182 enzymes: SgfI *
October 24, 2019 20:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 251 1650 1719 3325 4930 4957 5358 5394
Size: 251 1399 69 1606 1605 27 401 36
Fragments arranged by size:
1606 1605 1399 401 251 69 36 27
AccI GT'mk_AC
Cuts at: 0 753 1085 5394
Size: 753 332 4309
Fragments arranged by size:
4309 753 332
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 659 2098 2810 4625 5394
Size: 659 1439 712 1815 769
Fragments arranged by size:
1815 1439 769 712 659
AflIII A'CryG_T
Cuts at: 0 758 946 1326 5394
Size: 758 188 380 4068
Fragments arranged by size:
4068 758 380 188
AleI CACnn'nnGTG
Cuts at: 0 3347 3459 4615 5394
Size: 3347 112 1156 779
Fragments arranged by size:
3347 1156 779 112
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2027 2670 3437 5006 5394
Size: 2027 643 767 1569 388
Fragments arranged by size:
2027 1569 767 643 388
AlwNI CAG_nnn'CTG
Cuts at: 0 39 714 1435 1821 1939 2406 2525 3692
Size: 39 675 721 386 118 467 119 1167
Cuts at: 3692 4662 4758 4888 4914 5394
Size: 970 96 130 26 480
Fragments arranged by size:
1167 970 721 675 480 467 386 130
119 118 96 39 26
ApaI G_GGCC'C
Cuts at: 0 3220 4368 4473 5394
Size: 3220 1148 105 921
Fragments arranged by size:
3220 1148 921 105
ApaLI G'TGCA_C
Cuts at: 0 875 916 5394
Size: 875 41 4478
Fragments arranged by size:
4478 875 41
AvaI C'yCGr_G
Cuts at: 0 1721 1762 3835 5213 5394
Size: 1721 41 2073 1378 181
Fragments arranged by size:
2073 1721 1378 181 41
BamHI G'GATC_C
Cuts at: 0 1206 1465 1590 2985 5394
Size: 1206 259 125 1395 2409
Fragments arranged by size:
2409 1395 1206 259 125
BanI G'GyrC_C
Cuts at: 0 281 891 1148 1971 2262 2335 2429 2563
Size: 281 610 257 823 291 73 94 134
Cuts at: 2563 2945 3673 3736 3982 4233 4273 4867 5269
Size: 382 728 63 246 251 40 594 402
Cuts at: 5269 5394
Size: 125
Fragments arranged by size:
823 728 610 594 402 382 291 281
257 251 246 134 125 94 73 63
40
BanII G_rGCy'C
Cuts at: 0 1163 1376 1886 3220 3392 4368 4473 4628
Size: 1163 213 510 1334 172 976 105 155
Cuts at: 4628 4963 4980 5394
Size: 335 17 414
Fragments arranged by size:
1334 1163 976 510 414 335 213 172
155 105 17
BbeI G_GCGC'C
Cuts at: 0 285 1152 2433 5394
Size: 285 867 1281 2961
Fragments arranged by size:
2961 1281 867 285
BbvCI CC'TCA_GC
Cuts at: 0 905 1007 1712 3750 4047 4318 4420 4790
Size: 905 102 705 2038 297 271 102 370
Cuts at: 4790 5394
Size: 604
Fragments arranged by size:
2038 905 705 604 370 297 271 102
102
BciVI GTATCCnnnnn_n'
Cuts at: 0 397 5394
Size: 397 4997
BclI T'GATC_A
Cuts at: 0 2691 5121 5394
Size: 2691 2430 273
Fragments arranged by size:
2691 2430 273
BfrBI ATG'CAT
Cuts at: 0 3574 5394
Size: 3574 1820
BglI GCCn_nnn'nGGC
Cuts at: 0 91 212 889 1361 1520 1529 2477 2597
Size: 91 121 677 472 159 9 948 120
Cuts at: 2597 2873 3353 4846 5089 5394
Size: 276 480 1493 243 305
Fragments arranged by size:
1493 948 677 480 472 305 276 243
159 121 120 91 9
BglII A'GATC_T
Cuts at: 0 5375 5394
Size: 5375 19
BlpI GC'TnA_GC
Cuts at: 0 70 492 1375 4646 5093 5150 5394
Size: 70 422 883 3271 447 57 244
Fragments arranged by size:
3271 883 447 422 244 70 57
Bme1580I G_kGCm'C
Cuts at: 0 879 894 920 1055 1339 1429 1612 1976
Size: 879 15 26 135 284 90 183 364
Cuts at: 1976 2106 2267 2340 2451 2514 2907 2915 3060
Size: 130 161 73 111 63 393 8 145
Cuts at: 3060 3133 3151 3196 3220 3240 3531 3741 4167
Size: 73 18 45 24 20 291 210 426
Cuts at: 4167 4236 4368 4398 4473 4851 4956 5073 5085
Size: 69 132 30 75 378 105 117 12
Cuts at: 5085 5295 5394
Size: 210 99
Fragments arranged by size:
879 426 393 378 364 291 284 210
210 183 161 145 135 132 130 117
111 105 99 90 75 73 73 69
63 45 30 26 24 20 18 15
12 8
BmgBI CAC'GTC
Cuts at: 0 1498 1575 5394
Size: 1498 77 3819
Fragments arranged by size:
3819 1498 77
BmrI ACTGGGnnnn_n'
Cuts at: 0 67 597 1300 1536 1590 3405 4366 5307
Size: 67 530 703 236 54 1815 961 941
Cuts at: 5307 5394
Size: 87
Fragments arranged by size:
1815 961 941 703 530 236 87 67
54
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 27 5394
Size: 27 5367
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 436 1843 2233 2438 3919 4852 5020 5317
Size: 436 1407 390 205 1481 933 168 297
Cuts at: 5317 5394
Size: 77
Fragments arranged by size:
1481 1407 933 436 390 297 205 168
77
Bpu10I CC'TnA_GC
Cuts at: 0 905 1007 1534 1712 1788 1895 3750 4047
Size: 905 102 527 178 76 107 1855 297
Cuts at: 4047 4198 4318 4420 4790 5234 5394
Size: 151 120 102 370 444 160
Fragments arranged by size:
1855 905 527 444 370 297 178 160
151 120 107 102 102 76
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1235 2068 2345 5394
Size: 1235 833 277 3049
Fragments arranged by size:
3049 1235 833 277
BsaI GGTCTCn'nnnn_
Cuts at: 0 1305 3395 5394
Size: 1305 2090 1999
Fragments arranged by size:
2090 1999 1305
BsaAI yAC'GTr
Cuts at: 0 838 949 4723 5394
Size: 838 111 3774 671
Fragments arranged by size:
3774 838 671 111
BsaBI GATnn'nnATC
Cuts at: 0 980 5394
Size: 980 4414
BsaHI Gr'CG_yC
Cuts at: 0 282 999 1149 2430 5394
Size: 282 717 150 1281 2964
Fragments arranged by size:
2964 1281 717 282 150
BsaWI w'CCGG_w
Cuts at: 0 4688 5394
Size: 4688 706
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 409 439 5394
Size: 409 30 4955
Fragments arranged by size:
4955 409 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2266 2293 3802 4849 5394
Size: 2266 27 1509 1047 545
Fragments arranged by size:
2266 1509 1047 545 27
BseYI C'CCAG_C
Cuts at: 0 218 1144 2105 2266 2846 3126 3669 3711
Size: 218 926 961 161 580 280 543 42
Cuts at: 3711 4635 4784 4841 5394
Size: 924 149 57 553
Fragments arranged by size:
961 926 924 580 553 543 280 218
161 149 57 42
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1271 1945 2517 2982 3096 3622 3742 3749
Size: 1271 674 572 465 114 526 120 7
Cuts at: 3749 4964 5162 5392 5394
Size: 1215 198 230 2
Fragments arranged by size:
1271 1215 674 572 526 465 230 198
120 114 7 2
BsiHKAI G_wGCw'C
Cuts at: 0 879 920 1163 1444 1736 2711 3392 3824
Size: 879 41 243 281 292 975 681 432
Cuts at: 3824 4092 4628 4963 5394
Size: 268 536 335 431
Fragments arranged by size:
975 879 681 536 432 431 335 292
281 268 243 41
Bsp1286I G_dGCh'C
Cuts at: 0 879 894 920 1055 1163 1339 1376 1429
Size: 879 15 26 135 108 176 37 53
Cuts at: 1429 1444 1612 1736 1886 1976 2106 2267 2340
Size: 15 168 124 150 90 130 161 73
Cuts at: 2340 2451 2514 2711 2907 2915 3060 3133 3151
Size: 111 63 197 196 8 145 73 18
Cuts at: 3151 3196 3220 3240 3392 3531 3741 3824 4092
Size: 45 24 20 152 139 210 83 268
Cuts at: 4092 4167 4236 4368 4398 4473 4628 4851 4956
Size: 75 69 132 30 75 155 223 105
Cuts at: 4956 4963 4980 5073 5085 5295 5394
Size: 7 17 93 12 210 99
Fragments arranged by size:
879 268 223 210 210 197 196 176
168 161 155 152 150 145 139 135
132 130 124 111 108 105 99 93
90 83 75 75 73 73 69 63
53 45 37 30 26 24 20 18
17 15 15 12 8 7
BspEI T'CCGG_A
Cuts at: 0 4688 5394
Size: 4688 706
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 167 251 998 1208 1553 1650 1719 1874
Size: 167 84 747 210 345 97 69 155
Cuts at: 1874 3131 3325 4930 4957 5358 5394
Size: 1257 194 1605 27 401 36
Fragments arranged by size:
1605 1257 747 401 345 210 194 167
155 97 84 69 36 27
BsrBI CCG'CTC
Cuts at: 0 1223 4950 5233 5289 5394
Size: 1223 3727 283 56 105
Fragments arranged by size:
3727 1223 283 105 56
BsrDI GCAATG_nn'
Cuts at: 0 1107 1357 1906 2551 2643 2723 3022 4332
Size: 1107 250 549 645 92 80 299 1310
Cuts at: 4332 5394
Size: 1062
Fragments arranged by size:
1310 1107 1062 645 549 299 250 92
80
BsrFI r'CCGG_y
Cuts at: 0 1400 2621 2771 2942 3352 4276 5394
Size: 1400 1221 150 171 410 924 1118
Fragments arranged by size:
1400 1221 1118 924 410 171 150
BssSI C'ACGA_G
Cuts at: 0 2503 3440 5394
Size: 2503 937 1954
Fragments arranged by size:
2503 1954 937
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1343 1971 3021 3651 4252 4758 5068 5394
Size: 1343 628 1050 630 601 506 310 326
Fragments arranged by size:
1343 1050 630 628 601 506 326 310
BstEII G'GTnAC_C
Cuts at: 0 396 1755 5394
Size: 396 1359 3639
Fragments arranged by size:
3639 1359 396
BstXI CCAn_nnnn'nTGG
Cuts at: 0 408 483 587 5394
Size: 408 75 104 4807
Fragments arranged by size:
4807 408 104 75
BstYI r'GATC_y
Cuts at: 0 794 1206 1465 1590 2985 4652 5375 5394
Size: 794 412 259 125 1395 1667 723 19
Fragments arranged by size:
1667 1395 794 723 412 259 125 19
BstZ17I GTA'TAC
Cuts at: 0 1086 5394
Size: 1086 4308
BtgI C'CryG_G
Cuts at: 0 149 580 784 1047 1241 2736 2746 3078
Size: 149 431 204 263 194 1495 10 332
Cuts at: 3078 3267 3531 5394
Size: 189 264 1863
Fragments arranged by size:
1863 1495 431 332 264 263 204 194
189 149 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 301 1419 2904 5394
Size: 301 1118 1485 2490
Fragments arranged by size:
2490 1485 1118 301
BtsI GCAGTG_nn'
Cuts at: 0 973 1623 2862 2970 3060 3151 3189 3196
Size: 973 650 1239 108 90 91 38 7
Cuts at: 3196 3342 3343 3421 3480 3761 3928 4889 5073
Size: 146 1 78 59 281 167 961 184
Cuts at: 5073 5394
Size: 321
Fragments arranged by size:
1239 973 961 650 321 281 184 167
146 108 91 90 78 59 38 7
1
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 930 965 1089 1124 1684 1719 5394
Size: 930 35 124 35 560 35 3675
Fragments arranged by size:
3675 930 560 124 35 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 3055 3892 4252 5394
Size: 3055 837 360 1142
Fragments arranged by size:
3055 1142 837 360
DrdI GACnn_nn'nnGTC
Cuts at: 0 3101 5394
Size: 3101 2293
EaeI y'GGCC_r
Cuts at: 0 504 1014 1079 1305 1521 2598 2623 2940
Size: 504 510 65 226 216 1077 25 317
Cuts at: 2940 3264 4151 4607 5394
Size: 324 887 456 787
Fragments arranged by size:
1077 887 787 510 504 456 324 317
226 216 65 25
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3381 5394
Size: 3381 2013
EcoICRI GAG'CTC
Cuts at: 0 1161 3390 4626 4961 5394
Size: 1161 2229 1236 335 433
Fragments arranged by size:
2229 1236 1161 433 335
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 436 659 1843 2098 2233 2438 2810 3919
Size: 436 223 1184 255 135 205 372 1109
Cuts at: 3919 4625 4852 5020 5317 5394
Size: 706 227 168 297 77
Fragments arranged by size:
1184 1109 706 436 372 297 255 227
223 205 168 135 77
EcoNI CCTnn'n_nnAGG
Cuts at: 0 4486 4570 4918 5394
Size: 4486 84 348 476
Fragments arranged by size:
4486 476 348 84
EcoO109I rG'GnC_Cy
Cuts at: 0 99 1197 1530 1890 1936 2742 2794 2922
Size: 99 1098 333 360 46 806 52 128
Cuts at: 2922 3216 3217 3310 4314 5142 5217 5394
Size: 294 1 93 1004 828 75 177
Fragments arranged by size:
1098 1004 828 806 360 333 294 177
128 99 93 75 52 46 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 279 3090 4149 5394
Size: 279 2811 1059 1245
Fragments arranged by size:
2811 1245 1059 279
FspI TGC'GCA
Cuts at: 0 2295 5341 5394
Size: 2295 3046 53
Fragments arranged by size:
3046 2295 53
HaeII r_GCGC'y
Cuts at: 0 285 1152 1752 2220 2433 2675 3630 5394
Size: 285 867 600 468 213 242 955 1764
Fragments arranged by size:
1764 955 867 600 468 285 242 213
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 914 946 3421 3453 4104 4136 5394
Size: 914 32 2475 32 651 32 1258
Fragments arranged by size:
2475 1258 914 651 32 32 32
HindIII A'AGCT_T
Cuts at: 0 2897 5394
Size: 2897 2497
KasI G'GCGC_C
Cuts at: 0 281 1148 2429 5394
Size: 281 867 1281 2965
Fragments arranged by size:
2965 1281 867 281
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1892 4967 5297 5306 5394
Size: 1892 3075 330 9 88
Fragments arranged by size:
3075 1892 330 88 9
MscI TGG'CCA
Cuts at: 0 1523 2600 3266 4153 4609 5394
Size: 1523 1077 666 887 456 785
Fragments arranged by size:
1523 1077 887 785 666 456
MslI CAynn'nnrTG
Cuts at: 0 481 590 1022 2349 2648 2834 3347 3425
Size: 481 109 432 1327 299 186 513 78
Cuts at: 3425 3459 4600 4615 5394
Size: 34 1141 15 779
Fragments arranged by size:
1327 1141 779 513 481 432 299 186
109 78 34 15
MspA1I CmG'CkG
Cuts at: 0 354 408 1064 1144 1511 2435 2588 3110
Size: 354 54 656 80 367 924 153 522
Cuts at: 3110 3233 3488 3639 4161 4322 4336 4392 4467
Size: 123 255 151 522 161 14 56 75
Cuts at: 4467 4645 4845 5100 5250 5394
Size: 178 200 255 150 144
Fragments arranged by size:
924 656 522 522 367 354 255 255
200 178 161 153 151 150 144 123
80 75 56 54 14
NaeI GCC'GGC
Cuts at: 0 1402 2623 2773 3354 4278 5394
Size: 1402 1221 150 581 924 1116
Fragments arranged by size:
1402 1221 1116 924 581 150
NarI GG'CG_CC
Cuts at: 0 282 1149 2430 5394
Size: 282 867 1281 2964
Fragments arranged by size:
2964 1281 867 282
NcoI C'CATG_G
Cuts at: 0 1047 1241 3078 3267 5394
Size: 1047 194 1837 189 2127
Fragments arranged by size:
2127 1837 1047 194 189
NdeI CA'TA_TG
Cuts at: 0 2757 5394
Size: 2757 2637
NgoMIV G'CCGG_C
Cuts at: 0 1400 2621 2771 3352 4276 5394
Size: 1400 1221 150 581 924 1118
Fragments arranged by size:
1400 1221 1118 924 581 150
NsiI A_TGCA'T
Cuts at: 0 3576 5394
Size: 3576 1818
NspI r_CATG'y
Cuts at: 0 520 1330 2014 2647 3119 3578 3702 4006
Size: 520 810 684 633 472 459 124 304
Cuts at: 4006 5394
Size: 1388
Fragments arranged by size:
1388 810 684 633 520 472 459 304
124
PasI CC'CwG_GG
Cuts at: 0 44 886 2797 2908 3406 3496 4863 5073
Size: 44 842 1911 111 498 90 1367 210
Cuts at: 5073 5394
Size: 321
Fragments arranged by size:
1911 1367 842 498 321 210 111 90
44
PciI A'CATG_T
Cuts at: 0 1326 5394
Size: 1326 4068
PflMI CCAn_nnn'nTGG
Cuts at: 0 1638 3184 3248 4662 5394
Size: 1638 1546 64 1414 732
Fragments arranged by size:
1638 1546 1414 732 64
PfoI T'CCnGG_A
Cuts at: 0 1688 1777 4997 5394
Size: 1688 89 3220 397
Fragments arranged by size:
3220 1688 397 89
PmlI CAC'GTG
Cuts at: 0 949 5394
Size: 949 4445
PpuMI rG'GwC_Cy
Cuts at: 0 1197 1890 2742 2922 5217 5394
Size: 1197 693 852 180 2295 177
Fragments arranged by size:
2295 1197 852 693 180 177
PshAI GACnn'nnGTC
Cuts at: 0 1259 5394
Size: 1259 4135
PspOMI G'GGCC_C
Cuts at: 0 3216 4364 4469 5394
Size: 3216 1148 105 925
Fragments arranged by size:
3216 1148 925 105
PspXI vC'TCGA_Gb
Cuts at: 0 3835 5394
Size: 3835 1559
PstI C_TGCA'G
Cuts at: 0 248 3487 4023 4391 4763 4968 5249 5394
Size: 248 3239 536 368 372 205 281 145
Fragments arranged by size:
3239 536 372 368 281 248 205 145
PvuII CAG'CTG
Cuts at: 0 408 1064 1144 3488 4322 4645 5100 5394
Size: 408 656 80 2344 834 323 455 294
Fragments arranged by size:
2344 834 656 455 408 323 294 80
SacI G_AGCT'C
Cuts at: 0 1163 3392 4628 4963 5394
Size: 1163 2229 1236 335 431
Fragments arranged by size:
2229 1236 1163 431 335
SanDI GG'GwC_CC
Cuts at: 0 2742 2922 5394
Size: 2742 180 2472
Fragments arranged by size:
2742 2472 180
SbfI CC_TGCA'GG
Cuts at: 0 248 5394
Size: 248 5146
ScaI AGT'ACT
Cuts at: 0 808 5394
Size: 808 4586
SexAI A'CCwGG_T
Cuts at: 0 4919 5394
Size: 4919 475
SfcI C'TryA_G
Cuts at: 0 244 693 1029 1804 3483 4019 4340 4387
Size: 244 449 336 775 1679 536 321 47
Cuts at: 4387 4727 4759 4964 5045 5245 5254 5394
Size: 340 32 205 81 200 9 140
Fragments arranged by size:
1679 775 536 449 340 336 321 244
205 200 140 81 47 32 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 212 1529 5394
Size: 212 1317 3865
Fragments arranged by size:
3865 1317 212
SfoI GGC'GCC
Cuts at: 0 283 1150 2431 5394
Size: 283 867 1281 2963
Fragments arranged by size:
2963 1281 867 283
SmaI CCC'GGG
Cuts at: 0 1764 5394
Size: 1764 3630
SmlI C'TyrA_G
Cuts at: 0 12 1250 2047 2360 3835 5394
Size: 12 1238 797 313 1475 1559
Fragments arranged by size:
1559 1475 1238 797 313 12
SphI G_CATG'C
Cuts at: 0 2647 3578 4006 5394
Size: 2647 931 428 1388
Fragments arranged by size:
2647 1388 931 428
SspI AAT'ATT
Cuts at: 0 2842 5394
Size: 2842 2552
StuI AGG'CCT
Cuts at: 0 2290 5394
Size: 2290 3104
StyI C'CwwG_G
Cuts at: 0 1047 1193 1241 1976 1992 3078 3267 5163
Size: 1047 146 48 735 16 1086 189 1896
Cuts at: 5163 5394
Size: 231
Fragments arranged by size:
1896 1086 1047 735 231 189 146 48
16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1072 1567 2677 3308 3608 3661 3970 5107
Size: 1072 495 1110 631 300 53 309 1137
Cuts at: 5107 5394
Size: 287
Fragments arranged by size:
1137 1110 1072 631 495 309 300 287
53
TatI w'GTAC_w
Cuts at: 0 806 4818 5394
Size: 806 4012 576
Fragments arranged by size:
4012 806 576
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1582 1614 3030 3062 4926 4958 5394
Size: 1582 32 1416 32 1864 32 436
Fragments arranged by size:
1864 1582 1416 436 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1639 1957 3067 3529 3889 4165 4509 5394
Size: 1639 318 1110 462 360 276 344 885
Fragments arranged by size:
1639 1110 885 462 360 344 318 276
XhoI C'TCGA_G
Cuts at: 0 3835 5394
Size: 3835 1559
XmaI C'CCGG_G
Cuts at: 0 1762 5394
Size: 1762 3632
XmnI GAAnn'nnTTC
Cuts at: 0 262 4561 5394
Size: 262 4299 833
Fragments arranged by size:
4299 833 262
Enzymes that do cut:
AarI AccI AcuI AflIII AleI AlfI AlwNI ApaI
ApaLI AvaI BamHI BanI BanII BbeI BbvCI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstEII BstXI
BstYI BstZ17I BtgI BtgZI BtsI CspCI DraIII DrdI
EaeI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI
HaeII Hin4I HindIII KasI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NsiI NspI PasI
PciI PflMI PfoI PmlI PpuMI PshAI PspOMI PspXI
PstI PvuII SacI SanDI SbfI ScaI SexAI SfcI
SfiI SfoI SmaI SmlI SphI SspI StuI StyI
TaqII TatI TstI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AloI
ApoI AscI AseI AsiSI AvrII BaeI BbsI BcgI
BmtI BsiEI BsiWI BsmI BsmBI BspHI BsrGI BssHII
BstBI Bsu36I ClaI DraI EagI EarI EcoRI EcoRV
FseI FspAI HincII HpaI KpnI MfeI MluI NheI
NotI NruI PacI PmeI PpiI PsiI PsrI PvuI
RsrII SacII SalI SapI SgfI SgrAI SnaBI SpeI
SrfI SwaI Tth111I XbaI ZraI