Restriction map for
pF1KE6234
(Linear) (Six-base) MAPSORT of: KIEE6234.seq Check: 144 from: 1 to: 885
KIEE6234.orf
With 182 enzymes: SgfI *
July 20, 2018 17:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 385 885
Size: 385 500
Acc65I G'GTAC_C
Cuts at: 0 203 885
Size: 203 682
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 452 484 885
Size: 452 32 401
Fragments arranged by size:
452 401 32
BamHI G'GATC_C
Cuts at: 0 338 885
Size: 338 547
BanI G'GyrC_C
Cuts at: 0 203 270 609 885
Size: 203 67 339 276
Fragments arranged by size:
339 276 203 67
BanII G_rGCy'C
Cuts at: 0 129 457 885
Size: 129 328 428
Fragments arranged by size:
428 328 129
BbsI GAAGACnn'nnnn_
Cuts at: 0 65 513 705 874 885
Size: 65 448 192 169 11
Fragments arranged by size:
448 192 169 65 11
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 27 885
Size: 27 858
BclI T'GATC_A
Cuts at: 0 671 885
Size: 671 214
Bme1580I G_kGCm'C
Cuts at: 0 614 885
Size: 614 271
BmrI ACTGGGnnnn_n'
Cuts at: 0 456 885
Size: 456 429
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 38 885
Size: 38 847
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 479 868 885
Size: 479 389 17
Fragments arranged by size:
479 389 17
BsaI GGTCTCn'nnnn_
Cuts at: 0 9 257 885
Size: 9 248 628
Fragments arranged by size:
628 248 9
BsaAI yAC'GTr
Cuts at: 0 730 885
Size: 730 155
BsaWI w'CCGG_w
Cuts at: 0 341 581 885
Size: 341 240 304
Fragments arranged by size:
341 304 240
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 184 214 452 482 885
Size: 184 30 238 30 403
Fragments arranged by size:
403 238 184 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 40 43 46 49 52 421 463 484
Size: 40 3 3 3 3 369 42 21
Cuts at: 484 856 863 885
Size: 372 7 22
Fragments arranged by size:
372 369 42 40 22 21 7 3
3 3 3
BseYI C'CCAG_C
Cuts at: 0 28 885
Size: 28 857
BsiHKAI G_wGCw'C
Cuts at: 0 494 885
Size: 494 391
Bsp1286I G_dGCh'C
Cuts at: 0 129 457 494 614 885
Size: 129 328 37 120 271
Fragments arranged by size:
328 271 129 120 37
BspEI T'CCGG_A
Cuts at: 0 341 885
Size: 341 544
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 385 885
Size: 385 500
BsrDI GCAATG_nn'
Cuts at: 0 422 885
Size: 422 463
BsrFI r'CCGG_y
Cuts at: 0 645 885
Size: 645 240
BstYI r'GATC_y
Cuts at: 0 338 435 885
Size: 338 97 450
Fragments arranged by size:
450 338 97
EarI CTCTTCn'nnn_
Cuts at: 0 716 744 795 885
Size: 716 28 51 90
Fragments arranged by size:
716 90 51 28
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 479 868 885
Size: 479 389 17
Fragments arranged by size:
479 389 17
EcoNI CCTnn'n_nnAGG
Cuts at: 0 219 885
Size: 219 666
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 104 136 558 576 590 608 885
Size: 104 32 422 18 14 18 277
Fragments arranged by size:
422 277 104 32 18 18 14
HindIII A'AGCT_T
Cuts at: 0 6 885
Size: 6 879
KpnI G_GTAC'C
Cuts at: 0 207 885
Size: 207 678
MslI CAynn'nnrTG
Cuts at: 0 193 885
Size: 193 692
MspA1I CmG'CkG
Cuts at: 0 32 885
Size: 32 853
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 452 484 885
Size: 452 32 401
Fragments arranged by size:
452 401 32
PsiI TTA'TAA
Cuts at: 0 752 885
Size: 752 133
PstI C_TGCA'G
Cuts at: 0 503 536 885
Size: 503 33 349
Fragments arranged by size:
503 349 33
PvuII CAG'CTG
Cuts at: 0 32 885
Size: 32 853
SapI GCTCTTCn'nnn_
Cuts at: 0 716 885
Size: 716 169
SfcI C'TryA_G
Cuts at: 0 182 237 499 532 885
Size: 182 55 262 33 353
Fragments arranged by size:
353 262 182 55 33
SnaBI TAC'GTA
Cuts at: 0 730 885
Size: 730 155
StyI C'CwwG_G
Cuts at: 0 659 885
Size: 659 226
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 438 847 885
Size: 438 409 38
Fragments arranged by size:
438 409 38
TatI w'GTAC_w
Cuts at: 0 536 885
Size: 536 349
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 184 216 885
Size: 184 32 669
Fragments arranged by size:
669 184 32
Enzymes that do cut:
AarI Acc65I AloI BamHI BanI BanII BbsI BcgI
BclI Bme1580I BmrI BplI BpmI BsaI BsaAI BsaWI
BsaXI BseRI BseYI BsiHKAI Bsp1286I BspEI BspMI BsrDI
BsrFI BstYI EarI Eco57MI EcoNI Hin4I HindIII KpnI
MslI MspA1I PpiI PsiI PstI PvuII SapI SfcI
SnaBI StyI TaqII TatI TstI
Enzymes that do not cut:
AatII AccI AclI AcuI AfeI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BbeI BbvCI BciVI
BfrBI BglI BglII BlpI BmgBI BmtI Bpu10I BpuEI
BsaBI BsaHI BsgI BsiEI BsiWI BsmI BsmBI BspHI
BsrBI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EciI EcoICRI EcoO109I EcoRI
EcoRV FalI FseI FspI FspAI HaeII HincII HpaI
KasI MfeI MluI MmeI MscI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PfoI PmeI PmlI PpuMI PshAI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SmlI SpeI SphI SrfI SspI StuI SwaI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI