Restriction map for
pF1KE6626
(Linear) (Six-base) MAPSORT of: KIEE6626.seq Check: 8871 from: 1 to: 942
KIEE6626.orf
With 182 enzymes: SgfI *
June 20, 2019 10:05 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 595 942
Size: 595 347
AlwNI CAG_nnn'CTG
Cuts at: 0 753 942
Size: 753 189
ApaI G_GGCC'C
Cuts at: 0 155 942
Size: 155 787
AvaI C'yCGr_G
Cuts at: 0 442 942
Size: 442 500
BanI G'GyrC_C
Cuts at: 0 6 194 942
Size: 6 188 748
Fragments arranged by size:
748 188 6
BanII G_rGCy'C
Cuts at: 0 155 240 942
Size: 155 85 702
Fragments arranged by size:
702 155 85
BbeI G_GCGC'C
Cuts at: 0 10 198 942
Size: 10 188 744
Fragments arranged by size:
744 188 10
BbsI GAAGACnn'nnnn_
Cuts at: 0 701 942
Size: 701 241
BbvCI CC'TCA_GC
Cuts at: 0 761 942
Size: 761 181
BglI GCCn_nnn'nGGC
Cuts at: 0 835 942
Size: 835 107
Bme1580I G_kGCm'C
Cuts at: 0 155 942
Size: 155 787
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 200 394 514 942
Size: 178 22 194 120 428
Fragments arranged by size:
428 194 178 120 22
Bpu10I CC'TnA_GC
Cuts at: 0 761 942
Size: 761 181
BsaI GGTCTCn'nnnn_
Cuts at: 0 607 942
Size: 607 335
BsaHI Gr'CG_yC
Cuts at: 0 7 195 942
Size: 7 188 747
Fragments arranged by size:
747 188 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 130 181 289 316 391 427 442 490
Size: 130 51 108 27 75 36 15 48
Cuts at: 490 493 511 514 526 529 532 550 574
Size: 3 18 3 12 3 3 18 24
Cuts at: 574 610 942
Size: 36 332
Fragments arranged by size:
332 130 108 75 51 48 36 36
27 24 18 18 15 12 3 3
3 3
BseYI C'CCAG_C
Cuts at: 0 143 227 239 398 449 545 936 942
Size: 143 84 12 159 51 96 391 6
Fragments arranged by size:
391 159 143 96 84 51 12 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 942
Size: 91 851
BsiEI CG_ry'CG
Cuts at: 0 792 942
Size: 792 150
BsiHKAI G_wGCw'C
Cuts at: 0 689 942
Size: 689 253
Bsp1286I G_dGCh'C
Cuts at: 0 155 240 689 942
Size: 155 85 449 253
Fragments arranged by size:
449 253 155 85
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 761 942
Size: 761 181
BsrBI CCG'CTC
Cuts at: 0 432 483 942
Size: 432 51 459
Fragments arranged by size:
459 432 51
BsrFI r'CCGG_y
Cuts at: 0 91 942
Size: 91 851
Bsu36I CC'TnA_GG
Cuts at: 0 164 942
Size: 164 778
BtgI C'CryG_G
Cuts at: 0 833 942
Size: 833 109
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 855 942
Size: 855 87
BtsI GCAGTG_nn'
Cuts at: 0 103 942
Size: 103 839
EaeI y'GGCC_r
Cuts at: 0 93 827 836 942
Size: 93 734 9 106
Fragments arranged by size:
734 106 93 9
EarI CTCTTCn'nnn_
Cuts at: 0 61 942
Size: 61 881
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 200 394 514 595 942
Size: 178 22 194 120 81 347
Fragments arranged by size:
347 194 178 120 81 22
EcoNI CCTnn'n_nnAGG
Cuts at: 0 295 496 942
Size: 295 201 446
Fragments arranged by size:
446 295 201
EcoO109I rG'GnC_Cy
Cuts at: 0 152 204 750 942
Size: 152 52 546 192
Fragments arranged by size:
546 192 152 52
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 600 942
Size: 600 342
HaeII r_GCGC'y
Cuts at: 0 10 198 942
Size: 10 188 744
Fragments arranged by size:
744 188 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 616 648 942
Size: 616 32 294
Fragments arranged by size:
616 294 32
KasI G'GCGC_C
Cuts at: 0 6 194 942
Size: 6 188 748
Fragments arranged by size:
748 188 6
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 873 942
Size: 873 69
MscI TGG'CCA
Cuts at: 0 838 942
Size: 838 104
MspA1I CmG'CkG
Cuts at: 0 243 325 549 855 942
Size: 243 82 224 306 87
Fragments arranged by size:
306 243 224 87 82
NaeI GCC'GGC
Cuts at: 0 93 942
Size: 93 849
NarI GG'CG_CC
Cuts at: 0 7 195 942
Size: 7 188 747
Fragments arranged by size:
747 188 7
NgoMIV G'CCGG_C
Cuts at: 0 91 942
Size: 91 851
PflMI CCAn_nnn'nTGG
Cuts at: 0 246 942
Size: 246 696
PfoI T'CCnGG_A
Cuts at: 0 746 942
Size: 746 196
PpuMI rG'GwC_Cy
Cuts at: 0 204 750 942
Size: 204 546 192
Fragments arranged by size:
546 204 192
PshAI GACnn'nnGTC
Cuts at: 0 444 942
Size: 444 498
PspOMI G'GGCC_C
Cuts at: 0 151 942
Size: 151 791
PspXI vC'TCGA_Gb
Cuts at: 0 442 942
Size: 442 500
SfcI C'TryA_G
Cuts at: 0 220 942
Size: 220 722
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 835 942
Size: 835 107
SfoI GGC'GCC
Cuts at: 0 8 196 942
Size: 8 188 746
Fragments arranged by size:
746 188 8
SmlI C'TyrA_G
Cuts at: 0 442 942
Size: 442 500
StuI AGG'CCT
Cuts at: 0 189 583 942
Size: 189 394 359
Fragments arranged by size:
394 359 189
StyI C'CwwG_G
Cuts at: 0 677 942
Size: 677 265
XhoI C'TCGA_G
Cuts at: 0 442 942
Size: 442 500
Enzymes that do cut:
AcuI AlwNI ApaI AvaI BanI BanII BbeI BbsI
BbvCI BglI Bme1580I BpmI Bpu10I BsaI BsaHI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrFI
Bsu36I BtgI BtgZI BtsI EaeI EarI Eco57MI EcoNI
EcoO109I FalI HaeII Hin4I KasI MmeI MscI MspA1I
NaeI NarI NgoMIV PflMI PfoI PpuMI PshAI PspOMI
PspXI SfcI SfiI SfoI SmlI StuI StyI XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BcgI BciVI BclI
BfrBI BglII BlpI BmgBI BmrI BmtI BplI BpuEI
BsaAI BsaBI BsaWI BsaXI BsiWI BsmI BsmBI BspEI
BspHI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I ClaI CspCI DraI DraIII DrdI
EagI EciI EcoICRI EcoRI EcoRV FseI FspI FspAI
HincII HindIII HpaI KpnI MfeI MluI MslI NcoI
NdeI NheI NotI NruI NsiI NspI PacI PasI
PciI PmeI PmlI PpiI PsiI PsrI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI SwaI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI ZraI