(Linear) (Six-base) MAPSORT of: KIEE6682.seq  Check: 6728  from: 1  to: 942



KIEE6682.orf

 With 182 enzymes: SgfI * 

                        July 16, 2019 14:21  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     581     942 
   Size:        581     361
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     354     942 
   Size:        354     588
 
ApoI r'AATT_y

Cuts at:      0     317     393     412     583     679     942 
   Size:        317      76      19     171      96     263

  Fragments arranged by size: 

                 317     263     171      96      76      19
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     881     942 
   Size:        881      61
 
BclI T'GATC_A

Cuts at:      0     171     942 
   Size:        171     771
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      48     942 
   Size:         48     894
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      49     942 
   Size:         49     893
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      25     942 
   Size:         25     917
 
BseYI C'CCAG_C

Cuts at:      0     493     623     942 
   Size:        493     130     319

  Fragments arranged by size: 

                 493     319     130
 
BsiHKAI G_wGCw'C

Cuts at:      0     450     942 
   Size:        450     492
 
Bsp1286I G_dGCh'C

Cuts at:      0     450     942 
   Size:        450     492
 
BspHI T'CATG_A

Cuts at:      0     282     942 
   Size:        282     660
 
BsrFI r'CCGG_y

Cuts at:      0      34     942 
   Size:         34     908
 
BsrGI T'GTAC_A

Cuts at:      0     194     942 
   Size:        194     748
 
BstEII G'GTnAC_C

Cuts at:      0     546     942 
   Size:        546     396
 
BstYI r'GATC_y

Cuts at:      0     806     942 
   Size:        806     136
 
BtsI GCAGTG_nn'

Cuts at:      0     629     942 
   Size:        629     313
 
EarI CTCTTCn'nnn_

Cuts at:      0     509     648     942 
   Size:        509     139     294

  Fragments arranged by size: 

                 509     294     139
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      49     581     942 
   Size:         49     532     361

  Fragments arranged by size: 

                 532     361      49
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      64     942 
   Size:         64     878
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     798     830     942 
   Size:        798      32     112

  Fragments arranged by size: 

                 798     112      32
 
HincII GTy'rAC

Cuts at:      0     692     942 
   Size:        692     250
 
HindIII A'AGCT_T

Cuts at:      0      46     942 
   Size:         46     896
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      59     484     942 
   Size:         59     425     458

  Fragments arranged by size: 

                 458     425      59
 
MspA1I CmG'CkG

Cuts at:      0     778     942 
   Size:        778     164
 
NaeI GCC'GGC

Cuts at:      0      36     942 
   Size:         36     906
 
NgoMIV G'CCGG_C

Cuts at:      0      34     942 
   Size:         34     908
 
PsiI TTA'TAA

Cuts at:      0     619     942 
   Size:        619     323
 
PstI C_TGCA'G

Cuts at:      0     343     572     783     942 
   Size:        343     229     211     159

  Fragments arranged by size: 

                 343     229     211     159
 
PvuII CAG'CTG

Cuts at:      0     778     942 
   Size:        778     164
 
SapI GCTCTTCn'nnn_

Cuts at:      0     509     942 
   Size:        509     433
 
SfcI C'TryA_G

Cuts at:      0     339     568     779     942 
   Size:        339     229     211     163

  Fragments arranged by size: 

                 339     229     211     163
 
SspI AAT'ATT

Cuts at:      0     168     942 
   Size:        168     774
 
TatI w'GTAC_w

Cuts at:      0     194     942 
   Size:        194     748
 
XbaI T'CTAG_A

Cuts at:      0     468     942 
   Size:        468     474
 

 Enzymes that do cut:

     AcuI     AlfI     ApoI     BbsI     BclI     BmrI     BpmI    BseRI
    BseYI  BsiHKAI Bsp1286I    BspHI    BsrFI    BsrGI   BstEII    BstYI
     BtsI     EarI  Eco57MI     FalI    Hin4I   HincII  HindIII     MmeI
   MspA1I     NaeI   NgoMIV     PsiI     PstI    PvuII     SapI     SfcI
     SspI     TatI     XbaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI     BsgI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspMI    BsrBI    BsrDI   BssHII    BssSI   BstAPI
    BstBI    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII     HpaI
     KasI     KpnI     MfeI     MluI     MscI     MslI     NarI     NcoI
     NdeI     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     StuI     StyI
     SwaI    TaqII     TstI  Tth111I     XcmI     XhoI     XmaI     XmnI
     ZraI