Restriction map for
pF1KE6687
(Linear) (Six-base) MAPSORT of: KIEE6687.seq Check: 2143 from: 1 to: 696
KIEE6687.orf
With 182 enzymes: SgfI *
June 2, 2017 11:42 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 519 696
Size: 519 177
AfeI AGC'GCT
Cuts at: 0 7 544 640 696
Size: 7 537 96 56
Fragments arranged by size:
537 96 56 7
AflIII A'CryG_T
Cuts at: 0 476 696
Size: 476 220
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 436 696
Size: 436 260
AlwNI CAG_nnn'CTG
Cuts at: 0 321 696
Size: 321 375
ApaLI G'TGCA_C
Cuts at: 0 592 696
Size: 592 104
AvaI C'yCGr_G
Cuts at: 0 208 250 559 696
Size: 208 42 309 137
Fragments arranged by size:
309 208 137 42
AvrII C'CTAG_G
Cuts at: 0 18 696
Size: 18 678
BanI G'GyrC_C
Cuts at: 0 105 696
Size: 105 591
Bme1580I G_kGCm'C
Cuts at: 0 560 596 696
Size: 560 36 100
Fragments arranged by size:
560 100 36
Bpu10I CC'TnA_GC
Cuts at: 0 409 696
Size: 409 287
BsaAI yAC'GTr
Cuts at: 0 516 696
Size: 516 180
BsaBI GATnn'nnATC
Cuts at: 0 655 696
Size: 655 41
BseYI C'CCAG_C
Cuts at: 0 13 109 283 696
Size: 13 96 174 413
Fragments arranged by size:
413 174 96 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 299 696
Size: 299 397
BsiEI CG_ry'CG
Cuts at: 0 74 334 696
Size: 74 260 362
Fragments arranged by size:
362 260 74
BsiHKAI G_wGCw'C
Cuts at: 0 553 596 691 696
Size: 553 43 95 5
Fragments arranged by size:
553 95 43 5
BsmBI CGTCTCn'nnnn_
Cuts at: 0 181 696
Size: 181 515
Bsp1286I G_dGCh'C
Cuts at: 0 553 560 596 691 696
Size: 553 7 36 95 5
Fragments arranged by size:
553 95 36 7 5
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 128 696
Size: 128 568
BsrFI r'CCGG_y
Cuts at: 0 69 73 257 696
Size: 69 4 184 439
Fragments arranged by size:
439 184 69 4
BssSI C'ACGA_G
Cuts at: 0 546 690 696
Size: 546 144 6
Fragments arranged by size:
546 144 6
BstAPI GCAn_nnn'nTGC
Cuts at: 0 572 696
Size: 572 124
BstEII G'GTnAC_C
Cuts at: 0 24 696
Size: 24 672
BstYI r'GATC_y
Cuts at: 0 177 696
Size: 177 519
EaeI y'GGCC_r
Cuts at: 0 71 78 236 331 505 696
Size: 71 7 158 95 174 191
Fragments arranged by size:
191 174 158 95 71 7
EagI C'GGCC_G
Cuts at: 0 71 331 696
Size: 71 260 365
Fragments arranged by size:
365 260 71
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 88 319 696
Size: 88 231 377
Fragments arranged by size:
377 231 88
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 519 696
Size: 519 177
EcoO109I rG'GnC_Cy
Cuts at: 0 297 696
Size: 297 399
HaeII r_GCGC'y
Cuts at: 0 9 408 546 618 642 696
Size: 9 399 138 72 24 54
Fragments arranged by size:
399 138 72 54 24 9
MluI A'CGCG_T
Cuts at: 0 476 696
Size: 476 220
MscI TGG'CCA
Cuts at: 0 238 696
Size: 238 458
MspA1I CmG'CkG
Cuts at: 0 283 318 330 530 624 696
Size: 283 35 12 200 94 72
Fragments arranged by size:
283 200 94 72 35 12
NaeI GCC'GGC
Cuts at: 0 75 696
Size: 75 621
NgoMIV G'CCGG_C
Cuts at: 0 73 696
Size: 73 623
PasI CC'CwG_GG
Cuts at: 0 390 696
Size: 390 306
PpuMI rG'GwC_Cy
Cuts at: 0 297 696
Size: 297 399
PvuII CAG'CTG
Cuts at: 0 283 318 530 696
Size: 283 35 212 166
Fragments arranged by size:
283 212 166 35
StyI C'CwwG_G
Cuts at: 0 18 167 696
Size: 18 149 529
Fragments arranged by size:
529 149 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 123 161 696
Size: 123 38 535
Fragments arranged by size:
535 123 38
Enzymes that do cut:
AcuI AfeI AflIII AlfI AlwNI ApaLI AvaI AvrII
BanI Bme1580I Bpu10I BsaAI BsaBI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspMI BsrFI BssSI BstAPI BstEII
BstYI EaeI EagI EciI Eco57MI EcoO109I HaeII MluI
MscI MspA1I NaeI NgoMIV PasI PpuMI PvuII StyI
TaqII
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AhdI
AleI AloI ApaI ApoI AscI AseI AsiSI BaeI
BamHI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaHI BsaWI BsaXI BseRI BsiWI
BsmI BspEI BspHI BsrBI BsrDI BsrGI BssHII BstBI
BstXI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EarI EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI Hin4I HincII HindIII HpaI
KasI KpnI MfeI MmeI MslI NarI NcoI NdeI
NheI NotI NruI NsiI NspI PacI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI