Restriction map for
pF1KE6821
(Linear) (Six-base) MAPSORT of: KIEE6821.seq Check: 5729 from: 1 to: 1137
KIEE6821.orf
With 182 enzymes: SgfI *
June 19, 2019 14:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1004 1137
Size: 1004 133
Acc65I G'GTAC_C
Cuts at: 0 134 227 1137
Size: 134 93 910
Fragments arranged by size:
910 134 93
AgeI A'CCGG_T
Cuts at: 0 685 1137
Size: 685 452
AlwNI CAG_nnn'CTG
Cuts at: 0 242 387 591 1137
Size: 242 145 204 546
Fragments arranged by size:
546 242 204 145
ApaI G_GGCC'C
Cuts at: 0 54 291 1137
Size: 54 237 846
Fragments arranged by size:
846 237 54
ApoI r'AATT_y
Cuts at: 0 453 1137
Size: 453 684
BanI G'GyrC_C
Cuts at: 0 134 227 621 763 992 1050 1137
Size: 134 93 394 142 229 58 87
Fragments arranged by size:
394 229 142 134 93 87 58
BanII G_rGCy'C
Cuts at: 0 54 291 524 1088 1137
Size: 54 237 233 564 49
Fragments arranged by size:
564 237 233 54 49
BbsI GAAGACnn'nnnn_
Cuts at: 0 1045 1137
Size: 1045 92
BbvCI CC'TCA_GC
Cuts at: 0 200 1044 1137
Size: 200 844 93
Fragments arranged by size:
844 200 93
BglI GCCn_nnn'nGGC
Cuts at: 0 58 410 1137
Size: 58 352 727
Fragments arranged by size:
727 352 58
BglII A'GATC_T
Cuts at: 0 428 1137
Size: 428 709
Bme1580I G_kGCm'C
Cuts at: 0 54 291 766 1055 1137
Size: 54 237 475 289 82
Fragments arranged by size:
475 289 237 82 54
BmrI ACTGGGnnnn_n'
Cuts at: 0 1049 1137
Size: 1049 88
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 207 264 508 763 796 1137
Size: 207 57 244 255 33 341
Fragments arranged by size:
341 255 244 207 57 33
Bpu10I CC'TnA_GC
Cuts at: 0 200 1044 1082 1137
Size: 200 844 38 55
Fragments arranged by size:
844 200 55 38
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 391 874 904 1137
Size: 391 483 30 233
Fragments arranged by size:
483 391 233 30
BsaWI w'CCGG_w
Cuts at: 0 131 685 1137
Size: 131 554 452
Fragments arranged by size:
554 452 131
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 139 169 205 235 1137
Size: 139 30 36 30 902
Fragments arranged by size:
902 139 36 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 217 592 1078 1137
Size: 217 375 486 59
Fragments arranged by size:
486 375 217 59
BseYI C'CCAG_C
Cuts at: 0 165 420 677 1137
Size: 165 255 257 460
Fragments arranged by size:
460 257 255 165
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 596 1137
Size: 596 541
BsiEI CG_ry'CG
Cuts at: 0 338 1137
Size: 338 799
BsiHKAI G_wGCw'C
Cuts at: 0 125 146 524 1088 1137
Size: 125 21 378 564 49
Fragments arranged by size:
564 378 125 49 21
Bsp1286I G_dGCh'C
Cuts at: 0 54 125 146 291 524 766 1055 1088
Size: 54 71 21 145 233 242 289 33
Cuts at: 1088 1137
Size: 49
Fragments arranged by size:
289 242 233 145 71 54 49 33
21
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 572 957 1004 1137
Size: 572 385 47 133
Fragments arranged by size:
572 385 133 47
BsrDI GCAATG_nn'
Cuts at: 0 932 1137
Size: 932 205
BsrFI r'CCGG_y
Cuts at: 0 685 1137
Size: 685 452
BstBI TT'CG_AA
Cuts at: 0 537 1137
Size: 537 600
BstEII G'GTnAC_C
Cuts at: 0 90 688 1137
Size: 90 598 449
Fragments arranged by size:
598 449 90
BstYI r'GATC_y
Cuts at: 0 428 1137
Size: 428 709
BtgI C'CryG_G
Cuts at: 0 282 468 1137
Size: 282 186 669
Fragments arranged by size:
669 282 186
BtsI GCAGTG_nn'
Cuts at: 0 443 584 1137
Size: 443 141 553
Fragments arranged by size:
553 443 141
EarI CTCTTCn'nnn_
Cuts at: 0 874 1010 1137
Size: 874 136 127
Fragments arranged by size:
874 136 127
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 268 1137
Size: 268 869
EcoICRI GAG'CTC
Cuts at: 0 522 1086 1137
Size: 522 564 51
Fragments arranged by size:
564 522 51
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 207 264 508 763 796 1137
Size: 207 57 244 255 33 341
Fragments arranged by size:
341 255 244 207 57 33
EcoNI CCTnn'n_nnAGG
Cuts at: 0 199 379 1000 1137
Size: 199 180 621 137
Fragments arranged by size:
621 199 180 137
EcoO109I rG'GnC_Cy
Cuts at: 0 50 287 288 411 1137
Size: 50 237 1 123 726
Fragments arranged by size:
726 237 123 50 1
EcoRV GAT'ATC
Cuts at: 0 499 1137
Size: 499 638
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 81 1137
Size: 81 1056
KpnI G_GTAC'C
Cuts at: 0 138 231 1137
Size: 138 93 906
Fragments arranged by size:
906 138 93
MslI CAynn'nnrTG
Cuts at: 0 355 1137
Size: 355 782
MspA1I CmG'CkG
Cuts at: 0 165 424 588 756 1048 1137
Size: 165 259 164 168 292 89
Fragments arranged by size:
292 259 168 165 164 89
NcoI C'CATG_G
Cuts at: 0 468 1137
Size: 468 669
PasI CC'CwG_GG
Cuts at: 0 38 1137
Size: 38 1099
PflMI CCAn_nnn'nTGG
Cuts at: 0 474 1137
Size: 474 663
PfoI T'CCnGG_A
Cuts at: 0 662 773 1137
Size: 662 111 364
Fragments arranged by size:
662 364 111
PspOMI G'GGCC_C
Cuts at: 0 50 287 1137
Size: 50 237 850
Fragments arranged by size:
850 237 50
PstI C_TGCA'G
Cuts at: 0 164 193 438 954 1137
Size: 164 29 245 516 183
Fragments arranged by size:
516 245 183 164 29
PvuII CAG'CTG
Cuts at: 0 165 424 1137
Size: 165 259 713
Fragments arranged by size:
713 259 165
SacI G_AGCT'C
Cuts at: 0 524 1088 1137
Size: 524 564 49
Fragments arranged by size:
564 524 49
SexAI A'CCwGG_T
Cuts at: 0 137 1137
Size: 137 1000
SfcI C'TryA_G
Cuts at: 0 160 189 434 950 1137
Size: 160 29 245 516 187
Fragments arranged by size:
516 245 187 160 29
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 410 1137
Size: 410 727
SmlI C'TyrA_G
Cuts at: 0 406 853 883 1137
Size: 406 447 30 254
Fragments arranged by size:
447 406 254 30
StyI C'CwwG_G
Cuts at: 0 65 468 1137
Size: 65 403 669
Fragments arranged by size:
669 403 65
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 139 171 205 237 377 409 1137
Size: 139 32 34 32 140 32 728
Fragments arranged by size:
728 140 139 34 32 32 32
Enzymes that do cut:
AarI Acc65I AgeI AlwNI ApaI ApoI BanI BanII
BbsI BbvCI BglI BglII Bme1580I BmrI BpmI Bpu10I
BpuEI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspMI BsrDI BsrFI BstBI BstEII BstYI BtgI
BtsI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV
FalI KpnI MslI MspA1I NcoI PasI PflMI PfoI
PspOMI PstI PvuII SacI SexAI SfcI SfiI SmlI
StyI TstI
Enzymes that do not cut:
AatII AccI AclI AcuI AfeI AflII AflIII AhdI
AleI AlfI AloI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BcgI BciVI BclI BfrBI
BlpI BmgBI BmtI BplI BsaI BsaAI BsaBI BsaHI
BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrGI BssHII
BssSI BstAPI BstXI BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DraIII DrdI EaeI EagI EcoRI FseI FspI
FspAI HaeII Hin4I HincII HindIII HpaI KasI MfeI
MluI MmeI MscI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PciI PmeI PmlI
PpiI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII
SacII SalI SanDI SapI SbfI ScaI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI TaqII TatI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI