Restriction map for
pF1KE8021
(Linear) (Six-base) MAPSORT of: KIEE8021.seq Check: 4266 from: 1 to: 2223
KIEE8021.orf
With 182 enzymes: SgfI *
April 28, 2014 10:22 ..
AccI GT'mk_AC
Cuts at: 0 688 2223
Size: 688 1535
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 929 1669 1694 2173 2223
Size: 929 740 25 479 50
Fragments arranged by size:
929 740 479 50 25
AgeI A'CCGG_T
Cuts at: 0 18 2223
Size: 18 2205
AleI CACnn'nnGTG
Cuts at: 0 628 1708 2223
Size: 628 1080 515
Fragments arranged by size:
1080 628 515
AlwNI CAG_nnn'CTG
Cuts at: 0 165 2223
Size: 165 2058
ApaI G_GGCC'C
Cuts at: 0 256 2223
Size: 256 1967
ApoI r'AATT_y
Cuts at: 0 426 433 593 1276 2223
Size: 426 7 160 683 947
Fragments arranged by size:
947 683 426 160 7
AvaI C'yCGr_G
Cuts at: 0 1523 2223
Size: 1523 700
BamHI G'GATC_C
Cuts at: 0 552 2223
Size: 552 1671
BanII G_rGCy'C
Cuts at: 0 256 278 1952 2223
Size: 256 22 1674 271
Fragments arranged by size:
1674 271 256 22
BbsI GAAGACnn'nnnn_
Cuts at: 0 227 2086 2223
Size: 227 1859 137
Fragments arranged by size:
1859 227 137
BbvCI CC'TCA_GC
Cuts at: 0 1693 2223
Size: 1693 530
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1353 1387 2223
Size: 1353 34 836
Fragments arranged by size:
1353 836 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 545 2223
Size: 545 1678
BclI T'GATC_A
Cuts at: 0 2007 2223
Size: 2007 216
BfrBI ATG'CAT
Cuts at: 0 897 2223
Size: 897 1326
BglI GCCn_nnn'nGGC
Cuts at: 0 251 260 1380 2223
Size: 251 9 1120 843
Fragments arranged by size:
1120 843 251 9
BglII A'GATC_T
Cuts at: 0 1809 2223
Size: 1809 414
BlpI GC'TnA_GC
Cuts at: 0 1856 2223
Size: 1856 367
Bme1580I G_kGCm'C
Cuts at: 0 256 2223
Size: 256 1967
BmgBI CAC'GTC
Cuts at: 0 63 2223
Size: 63 2160
BmrI ACTGGGnnnn_n'
Cuts at: 0 282 1948 2223
Size: 282 1666 275
Fragments arranged by size:
1666 282 275
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 458 1157 2223
Size: 458 699 1066
Fragments arranged by size:
1066 699 458
Bpu10I CC'TnA_GC
Cuts at: 0 1693 2223
Size: 1693 530
BsaAI yAC'GTr
Cuts at: 0 561 607 2223
Size: 561 46 1616
Fragments arranged by size:
1616 561 46
BsaBI GATnn'nnATC
Cuts at: 0 1444 2223
Size: 1444 779
BsaWI w'CCGG_w
Cuts at: 0 18 2223
Size: 18 2205
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2071 2101 2223
Size: 2071 30 122
Fragments arranged by size:
2071 122 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1496 1658 1681 1768 2173 2223
Size: 1496 162 23 87 405 50
Fragments arranged by size:
1496 405 162 87 50 23
BseYI C'CCAG_C
Cuts at: 0 256 2223
Size: 256 1967
BsiEI CG_ry'CG
Cuts at: 0 8 2223
Size: 8 2215
BsiHKAI G_wGCw'C
Cuts at: 0 1952 2223
Size: 1952 271
BsmI GAATG_Cn'
Cuts at: 0 998 1468 1684 2138 2223
Size: 998 470 216 454 85
Fragments arranged by size:
998 470 454 216 85
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1508 2223
Size: 1508 715
Bsp1286I G_dGCh'C
Cuts at: 0 256 278 1952 2223
Size: 256 22 1674 271
Fragments arranged by size:
1674 271 256 22
BspHI T'CATG_A
Cuts at: 0 1224 1907 2223
Size: 1224 683 316
Fragments arranged by size:
1224 683 316
BsrDI GCAATG_nn'
Cuts at: 0 886 1369 2034 2223
Size: 886 483 665 189
Fragments arranged by size:
886 665 483 189
BsrFI r'CCGG_y
Cuts at: 0 18 2223
Size: 18 2205
BsrGI T'GTAC_A
Cuts at: 0 800 1236 2223
Size: 800 436 987
Fragments arranged by size:
987 800 436
BstYI r'GATC_y
Cuts at: 0 552 1191 1809 2223
Size: 552 639 618 414
Fragments arranged by size:
639 618 552 414
BstZ17I GTA'TAC
Cuts at: 0 689 2223
Size: 689 1534
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 459 494 2223
Size: 459 35 1729
Fragments arranged by size:
1729 459 35
DraIII CAC_nnn'GTG
Cuts at: 0 66 564 607 2223
Size: 66 498 43 1616
Fragments arranged by size:
1616 498 66 43
EaeI y'GGCC_r
Cuts at: 0 5 1474 2223
Size: 5 1469 749
Fragments arranged by size:
1469 749 5
EagI C'GGCC_G
Cuts at: 0 5 2223
Size: 5 2218
EarI CTCTTCn'nnn_
Cuts at: 0 266 647 1823 2223
Size: 266 381 1176 400
Fragments arranged by size:
1176 400 381 266
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 209 2223
Size: 209 2014
EcoICRI GAG'CTC
Cuts at: 0 1950 2223
Size: 1950 273
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 458 929 1157 1669 1694 2173 2223
Size: 458 471 228 512 25 479 50
Fragments arranged by size:
512 479 471 458 228 50 25
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2155 2223
Size: 2155 68
EcoO109I rG'GnC_Cy
Cuts at: 0 252 253 1429 2223
Size: 252 1 1176 794
Fragments arranged by size:
1176 794 252 1
EcoRI G'AATT_C
Cuts at: 0 426 2223
Size: 426 1797
EcoRV GAT'ATC
Cuts at: 0 1213 1905 2202 2223
Size: 1213 692 297 21
Fragments arranged by size:
1213 692 297 21
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1699 2223
Size: 1699 524
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1027 1059 1824 1856 1992 2024 2223
Size: 1027 32 765 32 136 32 199
Fragments arranged by size:
1027 765 199 136 32 32 32
HincII GTy'rAC
Cuts at: 0 572 2223
Size: 572 1651
HindIII A'AGCT_T
Cuts at: 0 1643 2223
Size: 1643 580
HpaI GTT'AAC
Cuts at: 0 572 2223
Size: 572 1651
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 1091 2223
Size: 130 961 1132
Fragments arranged by size:
1132 961 130
MslI CAynn'nnrTG
Cuts at: 0 628 1708 2223
Size: 628 1080 515
Fragments arranged by size:
1080 628 515
MspA1I CmG'CkG
Cuts at: 0 260 1764 1998 2223
Size: 260 1504 234 225
Fragments arranged by size:
1504 260 234 225
NdeI CA'TA_TG
Cuts at: 0 1471 2223
Size: 1471 752
NotI GC'GGCC_GC
Cuts at: 0 5 2223
Size: 5 2218
NsiI A_TGCA'T
Cuts at: 0 899 2223
Size: 899 1324
NspI r_CATG'y
Cuts at: 0 526 2223
Size: 526 1697
PfoI T'CCnGG_A
Cuts at: 0 80 548 1063 2223
Size: 80 468 515 1160
Fragments arranged by size:
1160 515 468 80
PpuMI rG'GwC_Cy
Cuts at: 0 1429 2223
Size: 1429 794
PspOMI G'GGCC_C
Cuts at: 0 252 2223
Size: 252 1971
PstI C_TGCA'G
Cuts at: 0 1381 2223
Size: 1381 842
PvuII CAG'CTG
Cuts at: 0 1998 2223
Size: 1998 225
SacI G_AGCT'C
Cuts at: 0 1952 2223
Size: 1952 271
SanDI GG'GwC_CC
Cuts at: 0 1429 2223
Size: 1429 794
SapI GCTCTTCn'nnn_
Cuts at: 0 266 2223
Size: 266 1957
SbfI CC_TGCA'GG
Cuts at: 0 1381 2223
Size: 1381 842
ScaI AGT'ACT
Cuts at: 0 668 2223
Size: 668 1555
SfcI C'TryA_G
Cuts at: 0 1325 1377 2223
Size: 1325 52 846
Fragments arranged by size:
1325 846 52
SmlI C'TyrA_G
Cuts at: 0 1523 2223
Size: 1523 700
SphI G_CATG'C
Cuts at: 0 526 2223
Size: 526 1697
SspI AAT'ATT
Cuts at: 0 867 2223
Size: 867 1356
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 636 1720 2223
Size: 636 1084 503
Fragments arranged by size:
1084 636 503
TatI w'GTAC_w
Cuts at: 0 666 800 939 1219 1236 1486 1519 1724
Size: 666 134 139 280 17 250 33 205
Cuts at: 1724 2223
Size: 499
Fragments arranged by size:
666 499 280 250 205 139 134 33
17
XhoI C'TCGA_G
Cuts at: 0 1523 2223
Size: 1523 700
XmnI GAAnn'nnTTC
Cuts at: 0 1682 2223
Size: 1682 541
Enzymes that do cut:
AccI AcuI AgeI AleI AlwNI ApaI ApoI AvaI
BamHI BanII BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BpmI Bpu10I
BsaAI BsaBI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspHI BsrDI BsrFI BsrGI BstYI
BstZ17I CspCI DraIII EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI Hin4I HincII
HindIII HpaI MmeI MslI MspA1I NdeI NotI NsiI
NspI PfoI PpuMI PspOMI PstI PvuII SacI SanDI
SapI SbfI ScaI SfcI SmlI SphI SspI TaqII
TatI XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AhdI
AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI
BanI BbeI BmtI BplI BpuEI BsaI BsaHI BsgI
BsiWI BspEI BspMI BsrBI BssHII BssSI BstAPI BstBI
BstEII BstXI Bsu36I BtgI BtgZI BtsI ClaI DraI
DrdI FseI FspI FspAI HaeII KasI KpnI MfeI
MluI MscI NaeI NarI NcoI NgoMIV NheI NruI
PacI PasI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SacII SalI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI
StuI StyI SwaI TstI Tth111I XbaI XcmI XmaI
ZraI