Restriction map for
pF1KE9251
(Linear) (Six-base) MAPSORT of: KIEE9251.seq Check: 1418 from: 1 to: 1683
KIEE9251.orf
With 182 enzymes: SgfI *
August 1, 2019 16:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1622 1683
Size: 1622 61
AccI GT'mk_AC
Cuts at: 0 650 1683
Size: 650 1033
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 289 574 818 1139 1271 1683
Size: 289 285 244 321 132 412
Fragments arranged by size:
412 321 289 285 244 132
AflIII A'CryG_T
Cuts at: 0 51 758 1683
Size: 51 707 925
Fragments arranged by size:
925 707 51
AleI CACnn'nnGTG
Cuts at: 0 1096 1683
Size: 1096 587
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 61 1683
Size: 61 1622
AlwNI CAG_nnn'CTG
Cuts at: 0 468 507 885 1267 1294 1569 1683
Size: 468 39 378 382 27 275 114
Fragments arranged by size:
468 382 378 275 114 39 27
AvaI C'yCGr_G
Cuts at: 0 101 409 1683
Size: 101 308 1274
Fragments arranged by size:
1274 308 101
AvrII C'CTAG_G
Cuts at: 0 1324 1683
Size: 1324 359
BamHI G'GATC_C
Cuts at: 0 1114 1683
Size: 1114 569
BanI G'GyrC_C
Cuts at: 0 658 1610 1683
Size: 658 952 73
Fragments arranged by size:
952 658 73
BanII G_rGCy'C
Cuts at: 0 114 123 410 416 974 1683
Size: 114 9 287 6 558 709
Fragments arranged by size:
709 558 287 114 9 6
BbsI GAAGACnn'nnnn_
Cuts at: 0 806 1662 1683
Size: 806 856 21
Fragments arranged by size:
856 806 21
BbvCI CC'TCA_GC
Cuts at: 0 1082 1130 1683
Size: 1082 48 553
Fragments arranged by size:
1082 553 48
BciVI GTATCCnnnnn_n'
Cuts at: 0 54 1683
Size: 54 1629
BlpI GC'TnA_GC
Cuts at: 0 806 1683
Size: 806 877
Bme1580I G_kGCm'C
Cuts at: 0 239 1683
Size: 239 1444
BmgBI CAC'GTC
Cuts at: 0 654 1683
Size: 654 1029
BmrI ACTGGGnnnn_n'
Cuts at: 0 800 873 1683
Size: 800 73 810
Fragments arranged by size:
810 800 73
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1188 1218 1451 1683
Size: 1188 30 233 232
Fragments arranged by size:
1188 233 232 30
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 128 345 409 416 617 974 1188 1207
Size: 128 217 64 7 201 357 214 19
Cuts at: 1207 1513 1564 1642 1683
Size: 306 51 78 41
Fragments arranged by size:
357 306 217 214 201 128 78 64
51 41 19 7
Bpu10I CC'TnA_GC
Cuts at: 0 404 931 1082 1130 1683
Size: 404 527 151 48 553
Fragments arranged by size:
553 527 404 151 48
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 512 1270 1459 1683
Size: 512 758 189 224
Fragments arranged by size:
758 512 224 189
BsaAI yAC'GTr
Cuts at: 0 1596 1683
Size: 1596 87
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 10 40 414 444 1683
Size: 10 30 374 30 1239
Fragments arranged by size:
1239 374 30 30 10
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 686 916 1683
Size: 686 230 767
Fragments arranged by size:
767 686 230
BseYI C'CCAG_C
Cuts at: 0 722 1063 1260 1683
Size: 722 341 197 423
Fragments arranged by size:
722 423 341 197
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 625 762 1576 1683
Size: 625 137 814 107
Fragments arranged by size:
814 625 137 107
BsiEI CG_ry'CG
Cuts at: 0 822 1683
Size: 822 861
BsiHKAI G_wGCw'C
Cuts at: 0 102 410 937 1056 1683
Size: 102 308 527 119 627
Fragments arranged by size:
627 527 308 119 102
BsmI GAATG_Cn'
Cuts at: 0 540 1683
Size: 540 1143
Bsp1286I G_dGCh'C
Cuts at: 0 102 114 123 239 410 416 937 974
Size: 102 12 9 116 171 6 521 37
Cuts at: 974 1056 1683
Size: 82 627
Fragments arranged by size:
627 521 171 116 102 82 37 12
9 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1622 1683
Size: 1622 61
BsrBI CCG'CTC
Cuts at: 0 1608 1683
Size: 1608 75
BsrFI r'CCGG_y
Cuts at: 0 817 1339 1628 1683
Size: 817 522 289 55
Fragments arranged by size:
817 522 289 55
BstXI CCAn_nnnn'nTGG
Cuts at: 0 426 1372 1683
Size: 426 946 311
Fragments arranged by size:
946 426 311
BstYI r'GATC_y
Cuts at: 0 559 672 1114 1359 1683
Size: 559 113 442 245 324
Fragments arranged by size:
559 442 324 245 113
BtgI C'CryG_G
Cuts at: 0 82 189 740 1421 1683
Size: 82 107 551 681 262
Fragments arranged by size:
681 551 262 107 82
BtsI GCAGTG_nn'
Cuts at: 0 843 948 1588 1683
Size: 843 105 640 95
Fragments arranged by size:
843 640 105 95
DraIII CAC_nnn'GTG
Cuts at: 0 1389 1683
Size: 1389 294
EaeI y'GGCC_r
Cuts at: 0 156 1515 1683
Size: 156 1359 168
Fragments arranged by size:
1359 168 156
EarI CTCTTCn'nnn_
Cuts at: 0 552 593 986 1070 1683
Size: 552 41 393 84 613
Fragments arranged by size:
613 552 393 84 41
EcoICRI GAG'CTC
Cuts at: 0 408 1683
Size: 408 1275
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 128 289 345 409 416 574 617 818
Size: 128 161 56 64 7 158 43 201
Cuts at: 818 974 1139 1188 1207 1271 1513 1564 1642
Size: 156 165 49 19 64 242 51 78
Cuts at: 1642 1683
Size: 41
Fragments arranged by size:
242 201 165 161 158 156 128 78
64 64 56 51 49 43 41 19
7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1176 1683
Size: 1176 507
EcoO109I rG'GnC_Cy
Cuts at: 0 1060 1328 1349 1539 1566 1683
Size: 1060 268 21 190 27 117
Fragments arranged by size:
1060 268 190 117 27 21
HaeII r_GCGC'y
Cuts at: 0 1267 1683
Size: 1267 416
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 10 42 1570 1602 1683
Size: 10 32 1528 32 81
Fragments arranged by size:
1528 81 32 32 10
MluI A'CGCG_T
Cuts at: 0 51 1683
Size: 51 1632
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 378 692 1683
Size: 378 314 991
Fragments arranged by size:
991 378 314
MscI TGG'CCA
Cuts at: 0 158 1683
Size: 158 1525
MslI CAynn'nnrTG
Cuts at: 0 307 1096 1481 1683
Size: 307 789 385 202
Fragments arranged by size:
789 385 307 202
MspA1I CmG'CkG
Cuts at: 0 67 84 1635 1683
Size: 67 17 1551 48
Fragments arranged by size:
1551 67 48 17
NcoI C'CATG_G
Cuts at: 0 189 740 1421 1683
Size: 189 551 681 262
Fragments arranged by size:
681 551 262 189
PasI CC'CwG_GG
Cuts at: 0 865 1119 1683
Size: 865 254 564
Fragments arranged by size:
865 564 254
PflMI CCAn_nnn'nTGG
Cuts at: 0 885 1267 1683
Size: 885 382 416
Fragments arranged by size:
885 416 382
PmlI CAC'GTG
Cuts at: 0 1596 1683
Size: 1596 87
PpuMI rG'GwC_Cy
Cuts at: 0 1060 1539 1683
Size: 1060 479 144
Fragments arranged by size:
1060 479 144
PspXI vC'TCGA_Gb
Cuts at: 0 409 1683
Size: 409 1274
PstI C_TGCA'G
Cuts at: 0 854 1278 1299 1683
Size: 854 424 21 384
Fragments arranged by size:
854 424 384 21
PvuII CAG'CTG
Cuts at: 0 1635 1683
Size: 1635 48
SacI G_AGCT'C
Cuts at: 0 410 1683
Size: 410 1273
SacII CC_GC'GG
Cuts at: 0 85 1683
Size: 85 1598
SfcI C'TryA_G
Cuts at: 0 647 850 1274 1295 1683
Size: 647 203 424 21 388
Fragments arranged by size:
647 424 388 203 21
SmlI C'TyrA_G
Cuts at: 0 409 527 1249 1438 1683
Size: 409 118 722 189 245
Fragments arranged by size:
722 409 245 189 118
SspI AAT'ATT
Cuts at: 0 1468 1683
Size: 1468 215
StuI AGG'CCT
Cuts at: 0 612 1683
Size: 612 1071
StyI C'CwwG_G
Cuts at: 0 189 740 1324 1421 1683
Size: 189 551 584 97 262
Fragments arranged by size:
584 551 262 189 97
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 646 736 1683
Size: 646 90 947
Fragments arranged by size:
947 646 90
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 138 170 1683
Size: 138 32 1513
Fragments arranged by size:
1513 138 32
Tth111I GACn'n_nGTC
Cuts at: 0 1582 1683
Size: 1582 101
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 237 1419 1683
Size: 237 1182 264
Fragments arranged by size:
1182 264 237
XhoI C'TCGA_G
Cuts at: 0 409 1683
Size: 409 1274
Enzymes that do cut:
AarI AccI AcuI AflIII AleI AlfI AlwNI AvaI
AvrII BamHI BanI BanII BbsI BbvCI BciVI BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaAI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspMI BsrBI BsrFI BstXI BstYI BtgI BtsI DraIII
EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I
MluI MmeI MscI MslI MspA1I NcoI PasI PflMI
PmlI PpuMI PspXI PstI PvuII SacI SacII SfcI
SmlI SspI StuI StyI TaqII TstI Tth111I XcmI
XhoI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AloI
ApaI ApaLI ApoI AscI AseI AsiSI BaeI BbeI
BcgI BclI BfrBI BglI BglII BmtI BsaI BsaBI
BsaHI BsaWI BsiWI BsmBI BspEI BspHI BsrDI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DrdI EagI EciI EcoRI EcoRV
FalI FseI FspI FspAI HincII HindIII HpaI KasI
KpnI MfeI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PciI PfoI PmeI PpiI
PshAI PsiI PspOMI PsrI PvuI RsrII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SwaI TatI XbaI
XmaI XmnI ZraI