Restriction map for
pF1KE9442
(Linear) (Six-base) MAPSORT of: KIEE9442.seq Check: 3124 from: 1 to: 2901
KIEE9442.orf
With 182 enzymes: SgfI *
October 24, 2019 20:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 272 2827 2901
Size: 272 2555 74
Fragments arranged by size:
2555 272 74
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 1164 1363 1511 2669 2901
Size: 226 938 199 148 1158 232
Fragments arranged by size:
1158 938 232 226 199 148
AflIII A'CryG_T
Cuts at: 0 611 2895 2901
Size: 611 2284 6
Fragments arranged by size:
2284 611 6
AhdI GACnn_n'nnGTC
Cuts at: 0 1877 2901
Size: 1877 1024
AleI CACnn'nnGTG
Cuts at: 0 1622 2901
Size: 1622 1279
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1039 2013 2901
Size: 1039 974 888
Fragments arranged by size:
1039 974 888
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 772 773 804 805 2901
Size: 772 1 31 1 2096
Fragments arranged by size:
2096 772 31 1 1
AlwNI CAG_nnn'CTG
Cuts at: 0 426 672 783 909 1578 2607 2901
Size: 426 246 111 126 669 1029 294
Fragments arranged by size:
1029 669 426 294 246 126 111
ApaI G_GGCC'C
Cuts at: 0 95 543 2549 2727 2901
Size: 95 448 2006 178 174
Fragments arranged by size:
2006 448 178 174 95
AvaI C'yCGr_G
Cuts at: 0 87 163 2413 2901
Size: 87 76 2250 488
Fragments arranged by size:
2250 488 87 76
AvrII C'CTAG_G
Cuts at: 0 2070 2901
Size: 2070 831
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2461 2494 2901
Size: 2461 33 407
Fragments arranged by size:
2461 407 33
BamHI G'GATC_C
Cuts at: 0 1406 2901
Size: 1406 1495
BanI G'GyrC_C
Cuts at: 0 70 940 976 1416 1816 2245 2901
Size: 70 870 36 440 400 429 656
Fragments arranged by size:
870 656 440 429 400 70 36
BanII G_rGCy'C
Cuts at: 0 20 95 164 543 1195 1589 1902 2351
Size: 20 75 69 379 652 394 313 449
Cuts at: 2351 2549 2587 2727 2901
Size: 198 38 140 174
Fragments arranged by size:
652 449 394 379 313 198 174 140
75 69 38 20
BbeI G_GCGC'C
Cuts at: 0 74 1820 2901
Size: 74 1746 1081
Fragments arranged by size:
1746 1081 74
BbsI GAAGACnn'nnnn_
Cuts at: 0 1499 1511 2901
Size: 1499 12 1390
Fragments arranged by size:
1499 1390 12
BbvCI CC'TCA_GC
Cuts at: 0 217 2374 2812 2901
Size: 217 2157 438 89
Fragments arranged by size:
2157 438 217 89
BfrBI ATG'CAT
Cuts at: 0 1338 2901
Size: 1338 1563
BglI GCCn_nnn'nGGC
Cuts at: 0 106 565 1705 2667 2901
Size: 106 459 1140 962 234
Fragments arranged by size:
1140 962 459 234 106
BglII A'GATC_T
Cuts at: 0 966 2901
Size: 966 1935
BlpI GC'TnA_GC
Cuts at: 0 1189 2901
Size: 1189 1712
Bme1580I G_kGCm'C
Cuts at: 0 95 543 2549 2727 2901
Size: 95 448 2006 178 174
Fragments arranged by size:
2006 448 178 174 95
BmrI ACTGGGnnnn_n'
Cuts at: 0 793 1616 2901
Size: 793 823 1285
Fragments arranged by size:
1285 823 793
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1269 2601 2901
Size: 1269 1332 300
Fragments arranged by size:
1332 1269 300
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 727 1009 1432 1573 1615 1628 2596 2692
Size: 727 282 423 141 42 13 968 96
Cuts at: 2692 2755 2901
Size: 63 146
Fragments arranged by size:
968 727 423 282 146 141 96 63
42 13
Bpu10I CC'TnA_GC
Cuts at: 0 217 2374 2812 2901
Size: 217 2157 438 89
Fragments arranged by size:
2157 438 217 89
BsaI GGTCTCn'nnnn_
Cuts at: 0 340 2668 2901
Size: 340 2328 233
Fragments arranged by size:
2328 340 233
BsaAI yAC'GTr
Cuts at: 0 2322 2898 2901
Size: 2322 576 3
Fragments arranged by size:
2322 576 3
BsaBI GATnn'nnATC
Cuts at: 0 2627 2901
Size: 2627 274
BsaHI Gr'CG_yC
Cuts at: 0 71 1817 2901
Size: 71 1746 1084
Fragments arranged by size:
1746 1084 71
BsaWI w'CCGG_w
Cuts at: 0 758 1229 2901
Size: 758 471 1672
Fragments arranged by size:
1672 758 471
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 433 463 2122 2152 2901
Size: 433 30 1659 30 749
Fragments arranged by size:
1659 749 433 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 292 480 1108 1528 2341 2901
Size: 292 188 628 420 813 560
Fragments arranged by size:
813 628 560 420 292 188
BseYI C'CCAG_C
Cuts at: 0 4 77 375 1102 1682 1904 2391 2653
Size: 4 73 298 727 580 222 487 262
Cuts at: 2653 2901
Size: 248
Fragments arranged by size:
727 580 487 298 262 248 222 73
4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 682 1093 1246 2014 2019 2901
Size: 682 411 153 768 5 882
Fragments arranged by size:
882 768 682 411 153 5
BsiEI CG_ry'CG
Cuts at: 0 2567 2901
Size: 2567 334
BsiHKAI G_wGCw'C
Cuts at: 0 164 1195 1589 1730 2587 2901
Size: 164 1031 394 141 857 314
Fragments arranged by size:
1031 857 394 314 164 141
BsmI GAATG_Cn'
Cuts at: 0 325 898 2901
Size: 325 573 2003
Fragments arranged by size:
2003 573 325
BsmBI CGTCTCn'nnnn_
Cuts at: 0 292 1903 2010 2016 2901
Size: 292 1611 107 6 885
Fragments arranged by size:
1611 885 292 107 6
Bsp1286I G_dGCh'C
Cuts at: 0 20 95 164 543 1195 1589 1730 1902
Size: 20 75 69 379 652 394 141 172
Cuts at: 1902 2351 2549 2587 2727 2901
Size: 449 198 38 140 174
Fragments arranged by size:
652 449 394 379 198 174 172 141
140 75 69 38 20
BspEI T'CCGG_A
Cuts at: 0 758 2901
Size: 758 2143
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 272 917 2279 2489 2827 2901
Size: 272 645 1362 210 338 74
Fragments arranged by size:
1362 645 338 272 210 74
BsrDI GCAATG_nn'
Cuts at: 0 1740 2901
Size: 1740 1161
BsrFI r'CCGG_y
Cuts at: 0 64 2242 2901
Size: 64 2178 659
Fragments arranged by size:
2178 659 64
BstAPI GCAn_nnn'nTGC
Cuts at: 0 426 2901
Size: 426 2475
BstXI CCAn_nnnn'nTGG
Cuts at: 0 576 636 987 2901
Size: 576 60 351 1914
Fragments arranged by size:
1914 576 351 60
BstYI r'GATC_y
Cuts at: 0 527 966 992 1209 1406 2628 2824 2901
Size: 527 439 26 217 197 1222 196 77
Fragments arranged by size:
1222 527 439 217 197 196 77 26
Bsu36I CC'TnA_GG
Cuts at: 0 1244 2168 2901
Size: 1244 924 733
Fragments arranged by size:
1244 924 733
BtgI C'CryG_G
Cuts at: 0 944 2198 2275 2309 2363 2792 2901
Size: 944 1254 77 34 54 429 109
Fragments arranged by size:
1254 944 429 109 77 54 34
BtsI GCAGTG_nn'
Cuts at: 0 117 1306 1486 1997 2002 2104 2470 2901
Size: 117 1189 180 511 5 102 366 431
Fragments arranged by size:
1189 511 431 366 180 117 102 5
EaeI y'GGCC_r
Cuts at: 0 752 1697 1985 2096 2240 2564 2901
Size: 752 945 288 111 144 324 337
Fragments arranged by size:
945 752 337 324 288 144 111
EagI C'GGCC_G
Cuts at: 0 2564 2901
Size: 2564 337
EarI CTCTTCn'nnn_
Cuts at: 0 259 2404 2491 2575 2901
Size: 259 2145 87 84 326
Fragments arranged by size:
2145 326 259 87 84
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 162 481 1681 2901
Size: 162 319 1200 1220
Fragments arranged by size:
1220 1200 319 162
EcoICRI GAG'CTC
Cuts at: 0 162 1193 1587 2585 2901
Size: 162 1031 394 998 316
Fragments arranged by size:
1031 998 394 316 162
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 727 1009 1164 1363 1432 1511 1573
Size: 226 501 282 155 199 69 79 62
Cuts at: 1573 1615 1628 2596 2669 2692 2755 2901
Size: 42 13 968 73 23 63 146
Fragments arranged by size:
968 501 282 226 199 155 146 79
73 69 63 62 42 23 13
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1643 2901
Size: 1643 1258
EcoO109I rG'GnC_Cy
Cuts at: 0 91 186 539 540 677 1529 1575 1648
Size: 91 95 353 1 137 852 46 73
Cuts at: 1648 2067 2135 2545 2546 2604 2723 2724 2901
Size: 419 68 410 1 58 119 1 177
Fragments arranged by size:
852 419 410 353 177 137 119 95
91 73 68 58 46 1 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1375 2901
Size: 1375 1526
HaeII r_GCGC'y
Cuts at: 0 28 74 400 1820 2901
Size: 28 46 326 1420 1081
Fragments arranged by size:
1420 1081 326 46 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 466 498 1501 1533 1863 1895 2051 2083
Size: 466 32 1003 32 330 32 156 32
Cuts at: 2083 2901
Size: 818
Fragments arranged by size:
1003 818 466 330 156 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 2642 2901
Size: 2642 259
KasI G'GCGC_C
Cuts at: 0 70 1816 2901
Size: 70 1746 1085
Fragments arranged by size:
1746 1085 70
MluI A'CGCG_T
Cuts at: 0 611 2901
Size: 611 2290
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1167 1937 2901
Size: 1167 770 964
Fragments arranged by size:
1167 964 770
MscI TGG'CCA
Cuts at: 0 754 2901
Size: 754 2147
MslI CAynn'nnrTG
Cuts at: 0 863 1505 1622 2901
Size: 863 642 117 1279
Fragments arranged by size:
1279 863 642 117
MspA1I CmG'CkG
Cuts at: 0 375 1050 1188 1299 1593 1904 2277 2901
Size: 375 675 138 111 294 311 373 624
Fragments arranged by size:
675 624 375 373 311 294 138 111
NaeI GCC'GGC
Cuts at: 0 66 2901
Size: 66 2835
NarI GG'CG_CC
Cuts at: 0 71 1817 2901
Size: 71 1746 1084
Fragments arranged by size:
1746 1084 71
NcoI C'CATG_G
Cuts at: 0 944 2309 2792 2901
Size: 944 1365 483 109
Fragments arranged by size:
1365 944 483 109
NgoMIV G'CCGG_C
Cuts at: 0 64 2901
Size: 64 2837
NsiI A_TGCA'T
Cuts at: 0 1340 2901
Size: 1340 1561
PasI CC'CwG_GG
Cuts at: 0 765 1533 2046 2187 2665 2901
Size: 765 768 513 141 478 236
Fragments arranged by size:
768 765 513 478 236 141
PflMI CCAn_nnn'nTGG
Cuts at: 0 672 885 1578 2607 2839 2901
Size: 672 213 693 1029 232 62
Fragments arranged by size:
1029 693 672 232 213 62
PfoI T'CCnGG_A
Cuts at: 0 55 458 953 1067 1409 2501 2901
Size: 55 403 495 114 342 1092 400
Fragments arranged by size:
1092 495 403 400 342 114 55
PmlI CAC'GTG
Cuts at: 0 2322 2898 2901
Size: 2322 576 3
Fragments arranged by size:
2322 576 3
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 289 321 2901
Size: 289 32 2580
Fragments arranged by size:
2580 289 32
PpuMI rG'GwC_Cy
Cuts at: 0 186 677 1575 2067 2901
Size: 186 491 898 492 834
Fragments arranged by size:
898 834 492 491 186
PsiI TTA'TAA
Cuts at: 0 728 2901
Size: 728 2173
PspOMI G'GGCC_C
Cuts at: 0 91 539 2545 2723 2901
Size: 91 448 2006 178 178
Fragments arranged by size:
2006 448 178 178 91
PstI C_TGCA'G
Cuts at: 0 365 422 566 740 779 2901
Size: 365 57 144 174 39 2122
Fragments arranged by size:
2122 365 174 144 57 39
PvuII CAG'CTG
Cuts at: 0 375 1050 1188 1299 1593 2901
Size: 375 675 138 111 294 1308
Fragments arranged by size:
1308 675 375 294 138 111
SacI G_AGCT'C
Cuts at: 0 164 1195 1589 2587 2901
Size: 164 1031 394 998 314
Fragments arranged by size:
1031 998 394 314 164
SacII CC_GC'GG
Cuts at: 0 2278 2901
Size: 2278 623
SanDI GG'GwC_CC
Cuts at: 0 677 2901
Size: 677 2224
SapI GCTCTTCn'nnn_
Cuts at: 0 2491 2575 2901
Size: 2491 84 326
Fragments arranged by size:
2491 326 84
SbfI CC_TGCA'GG
Cuts at: 0 566 2901
Size: 566 2335
ScaI AGT'ACT
Cuts at: 0 1949 2901
Size: 1949 952
SfcI C'TryA_G
Cuts at: 0 339 361 418 562 736 775 847 2901
Size: 339 22 57 144 174 39 72 2054
Fragments arranged by size:
2054 339 174 144 72 57 39 22
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1705 2901
Size: 1705 1196
SfoI GGC'GCC
Cuts at: 0 72 1818 2901
Size: 72 1746 1083
Fragments arranged by size:
1746 1083 72
SmaI CCC'GGG
Cuts at: 0 89 2901
Size: 89 2812
StuI AGG'CCT
Cuts at: 0 826 1249 1375 1471 2901
Size: 826 423 126 96 1430
Fragments arranged by size:
1430 826 423 126 96
StyI C'CwwG_G
Cuts at: 0 944 1382 2070 2309 2792 2901
Size: 944 438 688 239 483 109
Fragments arranged by size:
944 688 483 438 239 109
TatI w'GTAC_w
Cuts at: 0 1947 2261 2901
Size: 1947 314 640
Fragments arranged by size:
1947 640 314
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 762 1311 1938 2901
Size: 762 549 627 963
Fragments arranged by size:
963 762 627 549
XmaI C'CCGG_G
Cuts at: 0 87 2901
Size: 87 2814
XmnI GAAnn'nnTTC
Cuts at: 0 1496 2221 2901
Size: 1496 725 680
Fragments arranged by size:
1496 725 680
Enzymes that do cut:
AarI AcuI AflIII AhdI AleI AlfI AloI AlwNI
ApaI AvaI AvrII BaeI BamHI BanI BanII BbeI
BbsI BbvCI BfrBI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspMI BsrDI BsrFI BstAPI BstXI BstYI
Bsu36I BtgI BtsI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HindIII KasI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI PasI PflMI PfoI PmlI PpiI PpuMI
PsiI PspOMI PstI PvuII SacI SacII SanDI SapI
SbfI ScaI SfcI SfiI SfoI SmaI StuI StyI
TatI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI ApaLI
ApoI AscI AseI AsiSI BcgI BciVI BclI BmgBI
BmtI BpuEI BsiWI BspHI BsrBI BsrGI BssHII BssSI
BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI DraIII
DrdI EcoRI EcoRV FseI FspI FspAI HincII HpaI
KpnI MfeI NdeI NheI NotI NruI NspI PacI
PciI PmeI PshAI PspXI PsrI PvuI RsrII SalI
SexAI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI
SspI SwaI TaqII TstI Tth111I XbaI XhoI ZraI