Restriction map for
pF1KE9527
(Linear) (Six-base) MAPSORT of: KIEE9527.seq Check: 9726 from: 1 to: 1191
KIEE9527.orf
With 182 enzymes: SgfI *
August 25, 2016 10:58 ..
AccI GT'mk_AC
Cuts at: 0 242 1191
Size: 242 949
AflIII A'CryG_T
Cuts at: 0 473 713 1191
Size: 473 240 478
Fragments arranged by size:
478 473 240
AleI CACnn'nnGTG
Cuts at: 0 250 1191
Size: 250 941
AlwNI CAG_nnn'CTG
Cuts at: 0 778 1191
Size: 778 413
BanI G'GyrC_C
Cuts at: 0 70 1191
Size: 70 1121
BanII G_rGCy'C
Cuts at: 0 10 1191
Size: 10 1181
BbsI GAAGACnn'nnnn_
Cuts at: 0 219 1191
Size: 219 972
BciVI GTATCCnnnnn_n'
Cuts at: 0 1125 1191
Size: 1125 66
BclI T'GATC_A
Cuts at: 0 794 1191
Size: 794 397
BglI GCCn_nnn'nGGC
Cuts at: 0 349 1191
Size: 349 842
Bme1580I G_kGCm'C
Cuts at: 0 548 1191
Size: 548 643
BmgBI CAC'GTC
Cuts at: 0 141 1191
Size: 141 1050
BmtI G_CTAG'C
Cuts at: 0 49 1191
Size: 49 1142
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 534 1122 1191
Size: 534 588 69
Fragments arranged by size:
588 534 69
Bpu10I CC'TnA_GC
Cuts at: 0 692 1191
Size: 692 499
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 146 176 1191
Size: 146 30 1015
Fragments arranged by size:
1015 146 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 355 1191
Size: 355 836
BseYI C'CCAG_C
Cuts at: 0 10 27 759 1191
Size: 10 17 732 432
Fragments arranged by size:
732 432 17 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 532 1191
Size: 532 659
BsmI GAATG_Cn'
Cuts at: 0 806 1191
Size: 806 385
Bsp1286I G_dGCh'C
Cuts at: 0 10 548 1191
Size: 10 538 643
Fragments arranged by size:
643 538 10
BspHI T'CATG_A
Cuts at: 0 494 678 1191
Size: 494 184 513
Fragments arranged by size:
513 494 184
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 508 1191
Size: 508 683
BsrDI GCAATG_nn'
Cuts at: 0 566 1191
Size: 566 625
BsrGI T'GTAC_A
Cuts at: 0 974 1191
Size: 974 217
BstAPI GCAn_nnn'nTGC
Cuts at: 0 568 1191
Size: 568 623
BstEII G'GTnAC_C
Cuts at: 0 612 1191
Size: 612 579
BstXI CCAn_nnnn'nTGG
Cuts at: 0 145 1191
Size: 145 1046
BstYI r'GATC_y
Cuts at: 0 91 1191
Size: 91 1100
BtgI C'CryG_G
Cuts at: 0 539 818 1191
Size: 539 279 373
Fragments arranged by size:
539 373 279
BtsI GCAGTG_nn'
Cuts at: 0 72 1191
Size: 72 1119
DrdI GACnn_nn'nnGTC
Cuts at: 0 1024 1191
Size: 1024 167
EaeI y'GGCC_r
Cuts at: 0 341 737 878 1191
Size: 341 396 141 313
Fragments arranged by size:
396 341 313 141
EarI CTCTTCn'nnn_
Cuts at: 0 496 841 1164 1191
Size: 496 345 323 27
Fragments arranged by size:
496 345 323 27
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 534 1122 1191
Size: 534 588 69
Fragments arranged by size:
588 534 69
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 118 150 352 384 416 814 846 1191
Size: 118 32 202 32 32 398 32 345
Fragments arranged by size:
398 345 202 118 32 32 32 32
MfeI C'AATT_G
Cuts at: 0 236 248 1191
Size: 236 12 943
Fragments arranged by size:
943 236 12
MscI TGG'CCA
Cuts at: 0 343 739 880 1191
Size: 343 396 141 311
Fragments arranged by size:
396 343 311 141
MslI CAynn'nnrTG
Cuts at: 0 250 407 965 1191
Size: 250 157 558 226
Fragments arranged by size:
558 250 226 157
MspA1I CmG'CkG
Cuts at: 0 1010 1191
Size: 1010 181
NcoI C'CATG_G
Cuts at: 0 539 818 1191
Size: 539 279 373
Fragments arranged by size:
539 373 279
NheI G'CTAG_C
Cuts at: 0 45 1191
Size: 45 1146
NspI r_CATG'y
Cuts at: 0 477 717 1191
Size: 477 240 474
Fragments arranged by size:
477 474 240
PciI A'CATG_T
Cuts at: 0 473 713 1191
Size: 473 240 478
Fragments arranged by size:
478 473 240
PfoI T'CCnGG_A
Cuts at: 0 550 1138 1191
Size: 550 588 53
Fragments arranged by size:
588 550 53
PshAI GACnn'nnGTC
Cuts at: 0 223 1191
Size: 223 968
PstI C_TGCA'G
Cuts at: 0 67 1191
Size: 67 1124
PvuII CAG'CTG
Cuts at: 0 1010 1191
Size: 1010 181
SfcI C'TryA_G
Cuts at: 0 63 1191
Size: 63 1128
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 349 1191
Size: 349 842
StyI C'CwwG_G
Cuts at: 0 78 353 539 818 1191
Size: 78 275 186 279 373
Fragments arranged by size:
373 279 275 186 78
TatI w'GTAC_w
Cuts at: 0 476 974 1191
Size: 476 498 217
Fragments arranged by size:
498 476 217
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 351 1191
Size: 351 840
Enzymes that do cut:
AccI AflIII AleI AlwNI BanI BanII BbsI BciVI
BclI BglI Bme1580I BmgBI BmtI BpmI Bpu10I BsaXI
BseRI BseYI BsgI BsmI Bsp1286I BspHI BspMI BsrDI
BsrGI BstAPI BstEII BstXI BstYI BtgI BtsI DrdI
EaeI EarI Eco57MI Hin4I MfeI MscI MslI MspA1I
NcoI NheI NspI PciI PfoI PshAI PstI PvuII
SfcI SfiI StyI TatI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AgeI
AhdI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbeI BbvCI BcgI
BfrBI BglII BlpI BmrI BplI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsiEI BsiHKAI BsiWI BsmBI BspEI
BsrBI BsrFI BssHII BssSI BstBI BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DraIII EagI EciI EcoICRI EcoNI
EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HaeII
HincII HindIII HpaI KasI KpnI MluI MmeI NaeI
NarI NdeI NgoMIV NotI NruI NsiI PacI PasI
PflMI PmeI PmlI PpiI PpuMI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII
TstI Tth111I XbaI XhoI XmaI XmnI ZraI