Restriction map for
pF1KE9532
(Linear) (Six-base) MAPSORT of: KIEE9532.seq Check: 261 from: 1 to: 1110
KIEE9532.orf
With 182 enzymes: SgfI *
December 2, 2013 11:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1020 1110
Size: 1020 90
AatII G_ACGT'C
Cuts at: 0 482 1110
Size: 482 628
Acc65I G'GTAC_C
Cuts at: 0 1095 1110
Size: 1095 15
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 1110
Size: 37 1073
AflIII A'CryG_T
Cuts at: 0 398 1110
Size: 398 712
AleI CACnn'nnGTG
Cuts at: 0 160 1110
Size: 160 950
ApaI G_GGCC'C
Cuts at: 0 528 637 1110
Size: 528 109 473
Fragments arranged by size:
528 473 109
AvaI C'yCGr_G
Cuts at: 0 142 744 752 1110
Size: 142 602 8 358
Fragments arranged by size:
602 358 142 8
BanI G'GyrC_C
Cuts at: 0 127 1095 1110
Size: 127 968 15
Fragments arranged by size:
968 127 15
BanII G_rGCy'C
Cuts at: 0 8 79 149 170 197 528 568 637
Size: 8 71 70 21 27 331 40 69
Cuts at: 637 751 1110
Size: 114 359
Fragments arranged by size:
359 331 114 71 70 69 40 27
21 8
BbeI G_GCGC'C
Cuts at: 0 131 1110
Size: 131 979
BbsI GAAGACnn'nnnn_
Cuts at: 0 253 834 1110
Size: 253 581 276
Fragments arranged by size:
581 276 253
BglI GCCn_nnn'nGGC
Cuts at: 0 144 523 1110
Size: 144 379 587
Fragments arranged by size:
587 379 144
Bme1580I G_kGCm'C
Cuts at: 0 325 474 528 637 1110
Size: 325 149 54 109 473
Fragments arranged by size:
473 325 149 109 54
BmgBI CAC'GTC
Cuts at: 0 488 1026 1065 1110
Size: 488 538 39 45
Fragments arranged by size:
538 488 45 39
BmrI ACTGGGnnnn_n'
Cuts at: 0 639 1110
Size: 639 471
Bpu10I CC'TnA_GC
Cuts at: 0 73 1110
Size: 73 1037
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 1110
Size: 133 977
BsaHI Gr'CG_yC
Cuts at: 0 128 479 829 1110
Size: 128 351 350 281
Fragments arranged by size:
351 350 281 128
BsaWI w'CCGG_w
Cuts at: 0 764 1110
Size: 764 346
BseYI C'CCAG_C
Cuts at: 0 861 1110
Size: 861 249
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 894 1048 1110
Size: 894 154 62
Fragments arranged by size:
894 154 62
BsiHKAI G_wGCw'C
Cuts at: 0 686 1110
Size: 686 424
BsmI GAATG_Cn'
Cuts at: 0 363 1110
Size: 363 747
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1084 1110
Size: 1084 26
Bsp1286I G_dGCh'C
Cuts at: 0 8 79 149 170 197 325 474 528
Size: 8 71 70 21 27 128 149 54
Cuts at: 528 568 637 686 751 1110
Size: 40 69 49 65 359
Fragments arranged by size:
359 149 128 71 70 69 65 54
49 40 27 21 8
BspHI T'CATG_A
Cuts at: 0 710 1110
Size: 710 400
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 779 1020 1110
Size: 779 241 90
Fragments arranged by size:
779 241 90
BsrDI GCAATG_nn'
Cuts at: 0 377 1052 1110
Size: 377 675 58
Fragments arranged by size:
675 377 58
BstAPI GCAn_nnn'nTGC
Cuts at: 0 108 998 1110
Size: 108 890 112
Fragments arranged by size:
890 112 108
BstEII G'GTnAC_C
Cuts at: 0 153 1110
Size: 153 957
BstXI CCAn_nnnn'nTGG
Cuts at: 0 435 1065 1110
Size: 435 630 45
Fragments arranged by size:
630 435 45
BstYI r'GATC_y
Cuts at: 0 608 1110
Size: 608 502
Bsu36I CC'TnA_GG
Cuts at: 0 229 620 1110
Size: 229 391 490
Fragments arranged by size:
490 391 229
BtgI C'CryG_G
Cuts at: 0 123 726 890 1099 1110
Size: 123 603 164 209 11
Fragments arranged by size:
603 209 164 123 11
DrdI GACnn_nn'nnGTC
Cuts at: 0 295 1110
Size: 295 815
EaeI y'GGCC_r
Cuts at: 0 176 284 346 515 893 1110
Size: 176 108 62 169 378 217
Fragments arranged by size:
378 217 176 169 108 62
EarI CTCTTCn'nnn_
Cuts at: 0 16 581 667 1110
Size: 16 565 86 443
Fragments arranged by size:
565 443 86 16
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 724 1110
Size: 724 386
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 1110
Size: 37 1073
EcoO109I rG'GnC_Cy
Cuts at: 0 525 633 1110
Size: 525 108 477
Fragments arranged by size:
525 477 108
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1002 1110
Size: 1002 108
FspI TGC'GCA
Cuts at: 0 1048 1110
Size: 1048 62
HaeII r_GCGC'y
Cuts at: 0 131 1110
Size: 131 979
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 423 455 1110
Size: 423 32 655
Fragments arranged by size:
655 423 32
HincII GTy'rAC
Cuts at: 0 930 1110
Size: 930 180
KasI G'GCGC_C
Cuts at: 0 127 1110
Size: 127 983
KpnI G_GTAC'C
Cuts at: 0 1099 1110
Size: 1099 11
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 168 1110
Size: 168 942
MscI TGG'CCA
Cuts at: 0 517 895 1110
Size: 517 378 215
Fragments arranged by size:
517 378 215
MslI CAynn'nnrTG
Cuts at: 0 160 241 433 1110
Size: 160 81 192 677
Fragments arranged by size:
677 192 160 81
MspA1I CmG'CkG
Cuts at: 0 935 1101 1110
Size: 935 166 9
Fragments arranged by size:
935 166 9
NarI GG'CG_CC
Cuts at: 0 128 1110
Size: 128 982
NspI r_CATG'y
Cuts at: 0 402 1110
Size: 402 708
PasI CC'CwG_GG
Cuts at: 0 918 1110
Size: 918 192
PciI A'CATG_T
Cuts at: 0 398 1110
Size: 398 712
PflMI CCAn_nnn'nTGG
Cuts at: 0 861 1110
Size: 861 249
PfoI T'CCnGG_A
Cuts at: 0 962 1110
Size: 962 148
PshAI GACnn'nnGTC
Cuts at: 0 832 1110
Size: 832 278
PspOMI G'GGCC_C
Cuts at: 0 524 633 1110
Size: 524 109 477
Fragments arranged by size:
524 477 109
PvuII CAG'CTG
Cuts at: 0 935 1110
Size: 935 175
SacII CC_GC'GG
Cuts at: 0 1102 1110
Size: 1102 8
SfcI C'TryA_G
Cuts at: 0 702 1110
Size: 702 408
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 523 1110
Size: 523 587
SfoI GGC'GCC
Cuts at: 0 129 1110
Size: 129 981
SmlI C'TyrA_G
Cuts at: 0 148 1110
Size: 148 962
SspI AAT'ATT
Cuts at: 0 261 1110
Size: 261 849
StuI AGG'CCT
Cuts at: 0 982 1110
Size: 982 128
StyI C'CwwG_G
Cuts at: 0 855 1058 1110
Size: 855 203 52
Fragments arranged by size:
855 203 52
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 525 1110
Size: 525 585
ZraI GAC'GTC
Cuts at: 0 480 1110
Size: 480 630
Enzymes that do cut:
AarI AatII Acc65I AcuI AflIII AleI ApaI AvaI
BanI BanII BbeI BbsI BglI Bme1580I BmgBI BmrI
Bpu10I BpuEI BsaHI BsaWI BseYI BsgI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BspMI BsrDI BstAPI BstEII BstXI
BstYI Bsu36I BtgI DrdI EaeI EarI EciI Eco57MI
EcoO109I FalI FspI HaeII Hin4I HincII KasI KpnI
MmeI MscI MslI MspA1I NarI NspI PasI PciI
PflMI PfoI PshAI PspOMI PvuII SacII SfcI SfiI
SfoI SmlI SspI StuI StyI XcmI ZraI
Enzymes that do not cut:
AccI AclI AfeI AflII AgeI AhdI AlfI AloI
AlwNI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbvCI BcgI BciVI BclI BfrBI BglII BlpI
BmtI BplI BpmI BsaI BsaAI BsaBI BsaXI BseRI
BsiEI BsiWI BspEI BsrBI BsrFI BsrGI BssHII BssSI
BstBI BstZ17I BtgZI BtsI ClaI CspCI DraI DraIII
EagI EcoICRI EcoNI EcoRI EcoRV FseI FspAI HindIII
HpaI MfeI MluI NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PmeI PmlI PpiI PpuMI
PsiI PspXI PsrI PstI PvuI RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SwaI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI