Restriction map for
pF1KE9630
(Linear) (Six-base) MAPSORT of: KIEE9630.seq Check: 24 from: 1 to: 3255
KIEE9630.orf
With 182 enzymes: SgfI *
July 3, 2012 19:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2038 3255
Size: 2038 1217
AccI GT'mk_AC
Cuts at: 0 1822 3255
Size: 1822 1433
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 7 349 578 1736 3065 3181 3255
Size: 7 342 229 1158 1329 116 74
Fragments arranged by size:
1329 1158 342 229 116 74 7
AflIII A'CryG_T
Cuts at: 0 2006 2540 3255
Size: 2006 534 715
Fragments arranged by size:
2006 715 534
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 371 3255
Size: 371 2884
AlwNI CAG_nnn'CTG
Cuts at: 0 1002 2107 3255
Size: 1002 1105 1148
Fragments arranged by size:
1148 1105 1002
ApaI G_GGCC'C
Cuts at: 0 3204 3255
Size: 3204 51
ApaLI G'TGCA_C
Cuts at: 0 661 1384 1855 3255
Size: 661 723 471 1400
Fragments arranged by size:
1400 723 661 471
ApoI r'AATT_y
Cuts at: 0 108 163 220 374 742 1240 1957 2335
Size: 108 55 57 154 368 498 717 378
Cuts at: 2335 2449 2488 3088 3255
Size: 114 39 600 167
Fragments arranged by size:
717 600 498 378 368 167 154 114
108 57 55 39
BanI G'GyrC_C
Cuts at: 0 1071 1770 1938 3255
Size: 1071 699 168 1317
Fragments arranged by size:
1317 1071 699 168
BanII G_rGCy'C
Cuts at: 0 768 1789 2153 2603 3204 3255
Size: 768 1021 364 450 601 51
Fragments arranged by size:
1021 768 601 450 364 51
BbsI GAAGACnn'nnnn_
Cuts at: 0 818 1009 3255
Size: 818 191 2246
Fragments arranged by size:
2246 818 191
BclI T'GATC_A
Cuts at: 0 632 3255
Size: 632 2623
BfrBI ATG'CAT
Cuts at: 0 1495 3255
Size: 1495 1760
BglII A'GATC_T
Cuts at: 0 2421 3255
Size: 2421 834
BlpI GC'TnA_GC
Cuts at: 0 2929 3255
Size: 2929 326
Bme1580I G_kGCm'C
Cuts at: 0 665 1388 1859 3204 3255
Size: 665 723 471 1345 51
Fragments arranged by size:
1345 723 665 471 51
BmrI ACTGGGnnnn_n'
Cuts at: 0 1345 1500 1769 2499 3255
Size: 1345 155 269 730 756
Fragments arranged by size:
1345 756 730 269 155
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1163 3255
Size: 1163 2092
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 602 3255
Size: 602 2653
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 446 2654 3255
Size: 446 2208 601
Fragments arranged by size:
2208 601 446
BsaAI yAC'GTr
Cuts at: 0 970 3208 3255
Size: 970 2238 47
Fragments arranged by size:
2238 970 47
BsaBI GATnn'nnATC
Cuts at: 0 3122 3255
Size: 3122 133
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1139 3044 3255
Size: 1139 1905 211
Fragments arranged by size:
1905 1139 211
BseYI C'CCAG_C
Cuts at: 0 992 1125 2146 3255
Size: 992 133 1021 1109
Fragments arranged by size:
1109 1021 992 133
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1387 1793 3255
Size: 1387 406 1462
Fragments arranged by size:
1462 1387 406
BsiHKAI G_wGCw'C
Cuts at: 0 665 1388 1859 2603 3255
Size: 665 723 471 744 652
Fragments arranged by size:
744 723 665 652 471
BsmI GAATG_Cn'
Cuts at: 0 2705 3255
Size: 2705 550
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2006 3255
Size: 2006 1249
Bsp1286I G_dGCh'C
Cuts at: 0 665 768 1388 1789 1859 2153 2603 3204
Size: 665 103 620 401 70 294 450 601
Cuts at: 3204 3255
Size: 51
Fragments arranged by size:
665 620 601 450 401 294 103 70
51
BspHI T'CATG_A
Cuts at: 0 1236 1412 3255
Size: 1236 176 1843
Fragments arranged by size:
1843 1236 176
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 687 2038 3255
Size: 687 1351 1217
Fragments arranged by size:
1351 1217 687
BsrDI GCAATG_nn'
Cuts at: 0 66 305 805 1142 2117 2308 3255
Size: 66 239 500 337 975 191 947
Fragments arranged by size:
975 947 500 337 239 191 66
BsrGI T'GTAC_A
Cuts at: 0 266 302 3255
Size: 266 36 2953
Fragments arranged by size:
2953 266 36
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1816 3255
Size: 1816 1439
BstXI CCAn_nnnn'nTGG
Cuts at: 0 280 3255
Size: 280 2975
BstYI r'GATC_y
Cuts at: 0 927 2127 2421 3255
Size: 927 1200 294 834
Fragments arranged by size:
1200 927 834 294
BtgI C'CryG_G
Cuts at: 0 273 2221 2941 3255
Size: 273 1948 720 314
Fragments arranged by size:
1948 720 314 273
BtsI GCAGTG_nn'
Cuts at: 0 1202 2589 3255
Size: 1202 1387 666
Fragments arranged by size:
1387 1202 666
ClaI AT'CG_AT
Cuts at: 0 2957 3255
Size: 2957 298
DraI TTT'AAA
Cuts at: 0 218 503 2459 3255
Size: 218 285 1956 796
Fragments arranged by size:
1956 796 285 218
DraIII CAC_nnn'GTG
Cuts at: 0 1255 3255
Size: 1255 2000
EaeI y'GGCC_r
Cuts at: 0 1199 3255
Size: 1199 2056
EarI CTCTTCn'nnn_
Cuts at: 0 98 621 1566 1712 3255
Size: 98 523 945 146 1543
Fragments arranged by size:
1543 945 523 146 98
EcoICRI GAG'CTC
Cuts at: 0 2601 3255
Size: 2601 654
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 7 349 578 602 1736 3065 3181 3255
Size: 7 342 229 24 1134 1329 116 74
Fragments arranged by size:
1329 1134 342 229 116 74 24 7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2045 3255
Size: 2045 1210
EcoO109I rG'GnC_Cy
Cuts at: 0 1652 2501 3200 3201 3255
Size: 1652 849 699 1 54
Fragments arranged by size:
1652 849 699 54 1
EcoRI G'AATT_C
Cuts at: 0 374 1240 2488 3255
Size: 374 866 1248 767
Fragments arranged by size:
1248 866 767 374
EcoRV GAT'ATC
Cuts at: 0 590 3255
Size: 590 2665
HaeII r_GCGC'y
Cuts at: 0 1012 3255
Size: 1012 2243
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 595 627 1250 1282 1786 1818 2265 2297
Size: 595 32 623 32 504 32 447 32
Cuts at: 2297 2797 2829 3255
Size: 500 32 426
Fragments arranged by size:
623 595 504 500 447 426 32 32
32 32 32
HindIII A'AGCT_T
Cuts at: 0 721 1837 3255
Size: 721 1116 1418
Fragments arranged by size:
1418 1116 721
MfeI C'AATT_G
Cuts at: 0 2341 3255
Size: 2341 914
MscI TGG'CCA
Cuts at: 0 1201 3255
Size: 1201 2054
MslI CAynn'nnrTG
Cuts at: 0 353 1417 1936 2545 2729 3007 3013 3255
Size: 353 1064 519 609 184 278 6 242
Fragments arranged by size:
1064 609 519 353 278 242 184 6
NcoI C'CATG_G
Cuts at: 0 273 2221 2941 3255
Size: 273 1948 720 314
Fragments arranged by size:
1948 720 314 273
NsiI A_TGCA'T
Cuts at: 0 1497 3255
Size: 1497 1758
NspI r_CATG'y
Cuts at: 0 45 352 2010 2544 2994 3255
Size: 45 307 1658 534 450 261
Fragments arranged by size:
1658 534 450 307 261 45
PasI CC'CwG_GG
Cuts at: 0 2415 2483 3255
Size: 2415 68 772
Fragments arranged by size:
2415 772 68
PciI A'CATG_T
Cuts at: 0 2006 2540 3255
Size: 2006 534 715
Fragments arranged by size:
2006 715 534
PflMI CCAn_nnn'nTGG
Cuts at: 0 1255 2483 2511 3255
Size: 1255 1228 28 744
Fragments arranged by size:
1255 1228 744 28
PfoI T'CCnGG_A
Cuts at: 0 531 3255
Size: 531 2724
PpuMI rG'GwC_Cy
Cuts at: 0 1652 2501 3255
Size: 1652 849 754
Fragments arranged by size:
1652 849 754
PshAI GACnn'nnGTC
Cuts at: 0 2840 3255
Size: 2840 415
PspOMI G'GGCC_C
Cuts at: 0 3200 3255
Size: 3200 55
PstI C_TGCA'G
Cuts at: 0 347 1113 2049 3255
Size: 347 766 936 1206
Fragments arranged by size:
1206 936 766 347
SacI G_AGCT'C
Cuts at: 0 2603 3255
Size: 2603 652
SanDI GG'GwC_CC
Cuts at: 0 2501 3255
Size: 2501 754
ScaI AGT'ACT
Cuts at: 0 1233 3255
Size: 1233 2022
SfcI C'TryA_G
Cuts at: 0 343 1109 2045 2604 3255
Size: 343 766 936 559 651
Fragments arranged by size:
936 766 651 559 343
SmlI C'TyrA_G
Cuts at: 0 461 2669 3255
Size: 461 2208 586
Fragments arranged by size:
2208 586 461
SnaBI TAC'GTA
Cuts at: 0 3208 3255
Size: 3208 47
SphI G_CATG'C
Cuts at: 0 352 2994 3255
Size: 352 2642 261
Fragments arranged by size:
2642 352 261
SspI AAT'ATT
Cuts at: 0 655 3111 3255
Size: 655 2456 144
Fragments arranged by size:
2456 655 144
StuI AGG'CCT
Cuts at: 0 1993 3255
Size: 1993 1262
StyI C'CwwG_G
Cuts at: 0 273 333 2221 2941 3255
Size: 273 60 1888 720 314
Fragments arranged by size:
1888 720 314 273 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1392 1788 3255
Size: 1392 396 1467
Fragments arranged by size:
1467 1392 396
TatI w'GTAC_w
Cuts at: 0 230 266 302 1231 3222 3255
Size: 230 36 36 929 1991 33
Fragments arranged by size:
1991 929 230 36 36 33
XbaI T'CTAG_A
Cuts at: 0 1476 3255
Size: 1476 1779
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1194 2374 3255
Size: 1194 1180 881
Fragments arranged by size:
1194 1180 881
XmnI GAAnn'nnTTC
Cuts at: 0 2738 3092 3255
Size: 2738 354 163
Fragments arranged by size:
2738 354 163
Enzymes that do cut:
AarI AccI AcuI AflIII AlfI AlwNI ApaI ApaLI
ApoI BanI BanII BbsI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaAI BsaBI BseRI
BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI
BsrDI BsrGI BstAPI BstXI BstYI BtgI BtsI ClaI
DraI DraIII EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV HaeII Hin4I HindIII MfeI MscI MslI
NcoI NsiI NspI PasI PciI PflMI PfoI PpuMI
PshAI PspOMI PstI SacI SanDI ScaI SfcI SmlI
SnaBI SphI SspI StuI StyI TaqII TatI XbaI
XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AleI
AloI AscI AseI AsiSI AvaI AvrII BaeI BamHI
BbeI BbvCI BcgI BciVI BglI BmgBI BmtI Bpu10I
BsaI BsaHI BsaWI BsaXI BsiEI BsiWI BspEI BsrBI
BsrFI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgZI
CspCI DrdI EagI EciI FalI FseI FspI FspAI
HincII HpaI KasI KpnI MluI MmeI MspA1I NaeI
NarI NdeI NgoMIV NheI NotI NruI PacI PmeI
PmlI PpiI PsiI PspXI PsrI PvuI PvuII RsrII
SacII SalI SapI SbfI SexAI SfiI SfoI SgfI
SgrAI SmaI SpeI SrfI SwaI TstI Tth111I XhoI
XmaI ZraI