Restriction map for
pF1KE9635
(Linear) (Six-base) MAPSORT of: KIEE9635.seq Check: 6998 from: 1 to: 4632
KIEE9635.orf
With 182 enzymes: SgfI *
May 29, 2012 11:57 ..
AatII G_ACGT'C
Cuts at: 0 774 2820 4632
Size: 774 2046 1812
Fragments arranged by size:
2046 1812 774
Acc65I G'GTAC_C
Cuts at: 0 3615 4632
Size: 3615 1017
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3046 3401 3700 4249 4632
Size: 3046 355 299 549 383
Fragments arranged by size:
3046 549 383 355 299
AflIII A'CryG_T
Cuts at: 0 363 3093 4632
Size: 363 2730 1539
Fragments arranged by size:
2730 1539 363
AleI CACnn'nnGTG
Cuts at: 0 90 1538 4632
Size: 90 1448 3094
Fragments arranged by size:
3094 1448 90
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 453 4632
Size: 453 4179
AlwNI CAG_nnn'CTG
Cuts at: 0 267 2889 3056 3636 3804 4632
Size: 267 2622 167 580 168 828
Fragments arranged by size:
2622 828 580 267 168 167
ApaI G_GGCC'C
Cuts at: 0 58 64 4632
Size: 58 6 4568
Fragments arranged by size:
4568 58 6
ApoI r'AATT_y
Cuts at: 0 303 356 530 1225 1297 1377 2368 2695
Size: 303 53 174 695 72 80 991 327
Cuts at: 2695 3159 3240 3692 3826 4085 4632
Size: 464 81 452 134 259 547
Fragments arranged by size:
991 695 547 464 452 327 303 259
174 134 81 80 72 53
AvaI C'yCGr_G
Cuts at: 0 49 57 88 733 4632
Size: 49 8 31 645 3899
Fragments arranged by size:
3899 645 49 31 8
AvrII C'CTAG_G
Cuts at: 0 2829 4632
Size: 2829 1803
BanI G'GyrC_C
Cuts at: 0 3615 4632
Size: 3615 1017
BanII G_rGCy'C
Cuts at: 0 58 64 505 2525 3332 3798 3850 4632
Size: 58 6 441 2020 807 466 52 782
Fragments arranged by size:
2020 807 782 466 441 58 52 6
BbsI GAAGACnn'nnnn_
Cuts at: 0 93 968 1508 4439 4589 4632
Size: 93 875 540 2931 150 43
Fragments arranged by size:
2931 875 540 150 93 43
BbvCI CC'TCA_GC
Cuts at: 0 1790 3191 3879 4632
Size: 1790 1401 688 753
Fragments arranged by size:
1790 1401 753 688
BciVI GTATCCnnnnn_n'
Cuts at: 0 268 2743 3733 4018 4083 4632
Size: 268 2475 990 285 65 549
Fragments arranged by size:
2475 990 549 285 268 65
BfrBI ATG'CAT
Cuts at: 0 2239 4632
Size: 2239 2393
BglI GCCn_nnn'nGGC
Cuts at: 0 68 207 4632
Size: 68 139 4425
Fragments arranged by size:
4425 139 68
BglII A'GATC_T
Cuts at: 0 377 4632
Size: 377 4255
BlpI GC'TnA_GC
Cuts at: 0 1580 4130 4632
Size: 1580 2550 502
Fragments arranged by size:
2550 1580 502
Bme1580I G_kGCm'C
Cuts at: 0 58 64 97 676 4632
Size: 58 6 33 579 3956
Fragments arranged by size:
3956 579 58 33 6
BmgBI CAC'GTC
Cuts at: 0 3094 4632
Size: 3094 1538
BmrI ACTGGGnnnn_n'
Cuts at: 0 339 1545 2919 3614 3963 4512 4632
Size: 339 1206 1374 695 349 549 120
Fragments arranged by size:
1374 1206 695 549 349 339 120
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 4270 4272 4632
Size: 4270 2 360
Fragments arranged by size:
4270 360 2
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1071 1530 1598 3107 3403 4051 4242 4632
Size: 1071 459 68 1509 296 648 191 390
Fragments arranged by size:
1509 1071 648 459 390 296 191 68
Bpu10I CC'TnA_GC
Cuts at: 0 1073 1790 3191 3849 3879 4632
Size: 1073 717 1401 658 30 753
Fragments arranged by size:
1401 1073 753 717 658 30
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2920 4237 4632
Size: 2920 1317 395
Fragments arranged by size:
2920 1317 395
BsaI GGTCTCn'nnnn_
Cuts at: 0 4152 4632
Size: 4152 480
BsaAI yAC'GTr
Cuts at: 0 256 4632
Size: 256 4376
BsaBI GATnn'nnATC
Cuts at: 0 2478 2731 4632
Size: 2478 253 1901
Fragments arranged by size:
2478 1901 253
BsaHI Gr'CG_yC
Cuts at: 0 771 2817 4236 4632
Size: 771 2046 1419 396
Fragments arranged by size:
2046 1419 771 396
BsaWI w'CCGG_w
Cuts at: 0 559 4632
Size: 559 4073
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2776 2806 3080 3110 4481 4511 4632
Size: 2776 30 274 30 1371 30 121
Fragments arranged by size:
2776 1371 274 121 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 751 1267 1369 1633 2923 4632
Size: 751 516 102 264 1290 1709
Fragments arranged by size:
1709 1290 751 516 264 102
BseYI C'CCAG_C
Cuts at: 0 66 109 113 678 1631 1723 2788 3552
Size: 66 43 4 565 953 92 1065 764
Cuts at: 3552 3797 4058 4162 4461 4632
Size: 245 261 104 299 171
Fragments arranged by size:
1065 953 764 565 299 261 245 171
104 92 66 43 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 4 676 1791 2788 3061 4632
Size: 4 672 1115 997 273 1571
Fragments arranged by size:
1571 1115 997 672 273 4
BsiEI CG_ry'CG
Cuts at: 0 196 4632
Size: 196 4436
BsiHKAI G_wGCw'C
Cuts at: 0 2525 2909 4632
Size: 2525 384 1723
Fragments arranged by size:
2525 1723 384
BsmI GAATG_Cn'
Cuts at: 0 2241 4632
Size: 2241 2391
BsmBI CGTCTCn'nnnn_
Cuts at: 0 762 2808 2824 4227 4632
Size: 762 2046 16 1403 405
Fragments arranged by size:
2046 1403 762 405 16
Bsp1286I G_dGCh'C
Cuts at: 0 58 64 97 505 676 2525 2909 3332
Size: 58 6 33 408 171 1849 384 423
Cuts at: 3332 3798 3850 4632
Size: 466 52 782
Fragments arranged by size:
1849 782 466 423 408 384 171 58
52 33 6
BspEI T'CCGG_A
Cuts at: 0 559 4632
Size: 559 4073
BspHI T'CATG_A
Cuts at: 0 1664 1689 4632
Size: 1664 25 2943
Fragments arranged by size:
2943 1664 25
BsrBI CCG'CTC
Cuts at: 0 4266 4632
Size: 4266 366
BsrDI GCAATG_nn'
Cuts at: 0 960 1283 4632
Size: 960 323 3349
Fragments arranged by size:
3349 960 323
BsrFI r'CCGG_y
Cuts at: 0 965 4632
Size: 965 3667
BssSI C'ACGA_G
Cuts at: 0 290 3131 4632
Size: 290 2841 1501
Fragments arranged by size:
2841 1501 290
BstAPI GCAn_nnn'nTGC
Cuts at: 0 654 2448 2676 2889 4632
Size: 654 1794 228 213 1743
Fragments arranged by size:
1794 1743 654 228 213
BstBI TT'CG_AA
Cuts at: 0 104 4632
Size: 104 4528
BstXI CCAn_nnnn'nTGG
Cuts at: 0 918 1553 3609 4516 4632
Size: 918 635 2056 907 116
Fragments arranged by size:
2056 918 907 635 116
BstYI r'GATC_y
Cuts at: 0 152 377 1638 2589 2996 4632
Size: 152 225 1261 951 407 1636
Fragments arranged by size:
1636 1261 951 407 225 152
Bsu36I CC'TnA_GG
Cuts at: 0 571 1346 3448 3740 4632
Size: 571 775 2102 292 892
Fragments arranged by size:
2102 892 775 571 292
BtgI C'CryG_G
Cuts at: 0 4017 4632
Size: 4017 615
BtsI GCAGTG_nn'
Cuts at: 0 16 503 954 1311 2864 4173 4632
Size: 16 487 451 357 1553 1309 459
Fragments arranged by size:
1553 1309 487 459 451 357 16
ClaI AT'CG_AT
Cuts at: 0 1866 2478 4632
Size: 1866 612 2154
Fragments arranged by size:
2154 1866 612
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1194 1229 3586 3621 4632
Size: 1194 35 2357 35 1011
Fragments arranged by size:
2357 1194 1011 35 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 928 4632
Size: 928 3704
EaeI y'GGCC_r
Cuts at: 0 8 193 1964 4632
Size: 8 185 1771 2668
Fragments arranged by size:
2668 1771 185 8
EagI C'GGCC_G
Cuts at: 0 193 4632
Size: 193 4439
EarI CTCTTCn'nnn_
Cuts at: 0 113 814 1346 2558 2756 2987 4225 4632
Size: 113 701 532 1212 198 231 1238 407
Fragments arranged by size:
1238 1212 701 532 407 231 198 113
EcoICRI GAG'CTC
Cuts at: 0 2523 4632
Size: 2523 2109
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1071 1530 1598 3046 3107 3401 3403 3700
Size: 1071 459 68 1448 61 294 2 297
Cuts at: 3700 4051 4242 4249 4632
Size: 351 191 7 383
Fragments arranged by size:
1448 1071 459 383 351 297 294 191
68 61 7 2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 3739 4632
Size: 3739 893
EcoO109I rG'GnC_Cy
Cuts at: 0 54 3075 3549 3633 4251 4632
Size: 54 3021 474 84 618 381
Fragments arranged by size:
3021 618 474 381 84 54
EcoRI G'AATT_C
Cuts at: 0 530 3159 3240 4632
Size: 530 2629 81 1392
Fragments arranged by size:
2629 1392 530 81
EcoRV GAT'ATC
Cuts at: 0 2476 4632
Size: 2476 2156
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2610 3348 4632
Size: 2610 738 1284
Fragments arranged by size:
2610 1284 738
FspI TGC'GCA
Cuts at: 0 2441 4632
Size: 2441 2191
HaeII r_GCGC'y
Cuts at: 0 43 4632
Size: 43 4589
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1008 1040 1273 1305 1988 2020 2776 2808
Size: 1008 32 233 32 683 32 756 32
Cuts at: 2808 4132 4164 4632
Size: 1324 32 468
Fragments arranged by size:
1324 1008 756 683 468 233 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 1800 2510 4632
Size: 1800 710 2122
Fragments arranged by size:
2122 1800 710
HindIII A'AGCT_T
Cuts at: 0 1988 2245 2291 2774 4632
Size: 1988 257 46 483 1858
Fragments arranged by size:
1988 1858 483 257 46
HpaI GTT'AAC
Cuts at: 0 1800 4632
Size: 1800 2832
KpnI G_GTAC'C
Cuts at: 0 3619 4632
Size: 3619 1013
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 811 3643 4632
Size: 811 2832 989
Fragments arranged by size:
2832 989 811
MscI TGG'CCA
Cuts at: 0 1966 4632
Size: 1966 2666
MslI CAynn'nnrTG
Cuts at: 0 90 222 1393 1538 4594 4632
Size: 90 132 1171 145 3056 38
Fragments arranged by size:
3056 1171 145 132 90 38
MspA1I CmG'CkG
Cuts at: 0 66 113 2544 3556 4062 4465 4523 4632
Size: 66 47 2431 1012 506 403 58 109
Fragments arranged by size:
2431 1012 506 403 109 66 58 47
NcoI C'CATG_G
Cuts at: 0 4017 4632
Size: 4017 615
NdeI CA'TA_TG
Cuts at: 0 1196 4632
Size: 1196 3436
NotI GC'GGCC_GC
Cuts at: 0 193 4632
Size: 193 4439
NsiI A_TGCA'T
Cuts at: 0 2241 4632
Size: 2241 2391
NspI r_CATG'y
Cuts at: 0 227 367 1188 1392 2096 4632
Size: 227 140 821 204 704 2536
Fragments arranged by size:
2536 821 704 227 204 140
PasI CC'CwG_GG
Cuts at: 0 1567 4632
Size: 1567 3065
PciI A'CATG_T
Cuts at: 0 363 4632
Size: 363 4269
PflMI CCAn_nnn'nTGG
Cuts at: 0 3056 3519 4632
Size: 3056 463 1113
Fragments arranged by size:
3056 1113 463
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 518 550 4632
Size: 518 32 4082
Fragments arranged by size:
4082 518 32
PshAI GACnn'nnGTC
Cuts at: 0 3891 4632
Size: 3891 741
PsiI TTA'TAA
Cuts at: 0 2183 4632
Size: 2183 2449
PspOMI G'GGCC_C
Cuts at: 0 54 60 4632
Size: 54 6 4572
Fragments arranged by size:
4572 54 6
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 3179 3211 4632
Size: 3179 32 1421
Fragments arranged by size:
3179 1421 32
PstI C_TGCA'G
Cuts at: 0 2672 2885 3083 3586 4481 4632
Size: 2672 213 198 503 895 151
Fragments arranged by size:
2672 895 503 213 198 151
PvuII CAG'CTG
Cuts at: 0 113 2544 3556 4062 4465 4523 4632
Size: 113 2431 1012 506 403 58 109
Fragments arranged by size:
2431 1012 506 403 113 109 58
SacI G_AGCT'C
Cuts at: 0 2525 4632
Size: 2525 2107
SapI GCTCTTCn'nnn_
Cuts at: 0 2558 4632
Size: 2558 2074
SbfI CC_TGCA'GG
Cuts at: 0 3083 4632
Size: 3083 1549
ScaI AGT'ACT
Cuts at: 0 328 348 4632
Size: 328 20 4284
Fragments arranged by size:
4284 328 20
SexAI A'CCwGG_T
Cuts at: 0 1552 4632
Size: 1552 3080
SfcI C'TryA_G
Cuts at: 0 2668 2881 3079 3582 4477 4632
Size: 2668 213 198 503 895 155
Fragments arranged by size:
2668 895 503 213 198 155
SmaI CCC'GGG
Cuts at: 0 59 4632
Size: 59 4573
SmlI C'TyrA_G
Cuts at: 0 2935 4216 4632
Size: 2935 1281 416
Fragments arranged by size:
2935 1281 416
SpeI A'CTAG_T
Cuts at: 0 1236 4632
Size: 1236 3396
SrfI GCCC'GGGC
Cuts at: 0 59 4632
Size: 59 4573
SspI AAT'ATT
Cuts at: 0 720 4632
Size: 720 3912
StyI C'CwwG_G
Cuts at: 0 1040 1453 1913 2829 4017 4328 4632
Size: 1040 413 460 916 1188 311 304
Fragments arranged by size:
1188 1040 916 460 413 311 304
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 38 4157 4632
Size: 38 4119 475
Fragments arranged by size:
4119 475 38
TatI w'GTAC_w
Cuts at: 0 326 346 620 4603 4632
Size: 326 20 274 3983 29
Fragments arranged by size:
3983 326 274 29 20
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3080 3112 3198 3230 4481 4513 4632
Size: 3080 32 86 32 1251 32 119
Fragments arranged by size:
3080 1251 119 86 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 4504 4632
Size: 4504 128
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 915 4632
Size: 915 3717
XmaI C'CCGG_G
Cuts at: 0 57 4632
Size: 57 4575
XmnI GAAnn'nnTTC
Cuts at: 0 530 2368 2612 4342 4376 4632
Size: 530 1838 244 1730 34 256
Fragments arranged by size:
1838 1730 530 256 244 34
ZraI GAC'GTC
Cuts at: 0 772 2818 4632
Size: 772 2046 1814
Fragments arranged by size:
2046 1814 772
Enzymes that do cut:
AatII Acc65I AcuI AflIII AleI AlfI AlwNI ApaI
ApoI AvaI AvrII BanI BanII BbsI BbvCI BciVI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtsI ClaI CspCI
DrdI EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MmeI MscI MslI MspA1I NcoI NdeI
NotI NsiI NspI PasI PciI PflMI PpiI PshAI
PsiI PspOMI PsrI PstI PvuII SacI SapI SbfI
ScaI SexAI SfcI SmaI SmlI SpeI SrfI SspI
StyI TaqII TatI TstI Tth111I XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AccI AclI AfeI AflII AgeI AhdI AloI
ApaLI AscI AseI AsiSI BaeI BamHI BbeI BcgI
BclI BmtI BsiWI BspMI BsrGI BssHII BstEII BstZ17I
BtgZI DraI DraIII EciI FseI FspAI KasI MfeI
MluI NaeI NarI NgoMIV NheI NruI PacI PfoI
PmeI PmlI PpuMI PspXI PvuI RsrII SacII SalI
SanDI SfiI SfoI SgfI SgrAI SnaBI SphI StuI
SwaI XbaI XhoI