Restriction map for
pF1KE9636
(Linear) (Six-base) MAPSORT of: KIEE9636.seq Check: 8861 from: 1 to: 4677
KIEE9636.orf
With 182 enzymes: SgfI *
May 29, 2012 11:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2757 3050 3561 4677
Size: 2757 293 511 1116
Fragments arranged by size:
2757 1116 511 293
AccI GT'mk_AC
Cuts at: 0 2034 2248 2532 4677
Size: 2034 214 284 2145
Fragments arranged by size:
2145 2034 284 214
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 171 2305 2414 3911 4580 4677
Size: 171 2134 109 1497 669 97
Fragments arranged by size:
2134 1497 669 171 109 97
AflIII A'CryG_T
Cuts at: 0 529 2134 4677
Size: 529 1605 2543
Fragments arranged by size:
2543 1605 529
AhdI GACnn_n'nnGTC
Cuts at: 0 2857 4677
Size: 2857 1820
AleI CACnn'nnGTG
Cuts at: 0 36 808 1580 4677
Size: 36 772 772 3097
Fragments arranged by size:
3097 772 772 36
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1507 2484 4042 4677
Size: 1507 977 1558 635
Fragments arranged by size:
1558 1507 977 635
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1252 1284 4677
Size: 1252 32 3393
Fragments arranged by size:
3393 1252 32
AlwNI CAG_nnn'CTG
Cuts at: 0 213 2071 2235 2706 2922 3222 3430 3576
Size: 213 1858 164 471 216 300 208 146
Cuts at: 3576 3798 3882 3988 4263 4462 4677
Size: 222 84 106 275 199 215
Fragments arranged by size:
1858 471 300 275 222 216 215 213
208 199 164 146 106 84
ApaI G_GGCC'C
Cuts at: 0 4188 4359 4516 4677
Size: 4188 171 157 161
Fragments arranged by size:
4188 171 161 157
ApoI r'AATT_y
Cuts at: 0 271 1339 3273 3718 4677
Size: 271 1068 1934 445 959
Fragments arranged by size:
1934 1068 959 445 271
AvaI C'yCGr_G
Cuts at: 0 404 989 1163 2477 3083 3814 4181 4222
Size: 404 585 174 1314 606 731 367 41
Cuts at: 4222 4346 4415 4677
Size: 124 69 262
Fragments arranged by size:
1314 731 606 585 404 367 262 174
124 69 41
BamHI G'GATC_C
Cuts at: 0 4295 4677
Size: 4295 382
BanI G'GyrC_C
Cuts at: 0 1097 1242 1376 1491 1605 1874 2739 2830
Size: 1097 145 134 115 114 269 865 91
Cuts at: 2830 2866 3749 4677
Size: 36 883 928
Fragments arranged by size:
1097 928 883 865 269 145 134 115
114 91 36
BanII G_rGCy'C
Cuts at: 0 292 405 2484 2555 3594 3929 4102 4188
Size: 292 113 2079 71 1039 335 173 86
Cuts at: 4188 4202 4347 4359 4450 4516 4677
Size: 14 145 12 91 66 161
Fragments arranged by size:
2079 1039 335 292 173 161 145 113
91 86 71 66 14 12
BbeI G_GCGC'C
Cuts at: 0 1878 3753 4677
Size: 1878 1875 924
Fragments arranged by size:
1878 1875 924
BbsI GAAGACnn'nnnn_
Cuts at: 0 677 1509 1597 1911 4677
Size: 677 832 88 314 2766
Fragments arranged by size:
2766 832 677 314 88
BbvCI CC'TCA_GC
Cuts at: 0 2651 2817 3655 4463 4664 4677
Size: 2651 166 838 808 201 13
Fragments arranged by size:
2651 838 808 201 166 13
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 101 135 3472 3506 4677
Size: 101 34 3337 34 1171
Fragments arranged by size:
3337 1171 101 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 3487 4677
Size: 3487 1190
BglI GCCn_nnn'nGGC
Cuts at: 0 1872 2174 2647 3807 3877 4677
Size: 1872 302 473 1160 70 800
Fragments arranged by size:
1872 1160 800 473 302 70
BglII A'GATC_T
Cuts at: 0 707 4113 4677
Size: 707 3406 564
Fragments arranged by size:
3406 707 564
BlpI GC'TnA_GC
Cuts at: 0 2072 3923 4677
Size: 2072 1851 754
Fragments arranged by size:
2072 1851 754
Bme1580I G_kGCm'C
Cuts at: 0 43 1102 1247 1496 1610 2385 2833 4188
Size: 43 1059 145 249 114 775 448 1355
Cuts at: 4188 4359 4516 4677
Size: 171 157 161
Fragments arranged by size:
1355 1059 775 448 249 171 161 157
145 114 43
BmgBI CAC'GTC
Cuts at: 0 532 4677
Size: 532 4145
BmrI ACTGGGnnnn_n'
Cuts at: 0 948 1587 3506 4511 4677
Size: 948 639 1919 1005 166
Fragments arranged by size:
1919 1005 948 639 166
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3267 4116 4361 4677
Size: 3267 849 245 316
Fragments arranged by size:
3267 849 316 245
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 527 892 1293 1572 2071 2202 2359 2667
Size: 527 365 401 279 499 131 157 308
Cuts at: 2667 2743 2989 3211 3258 3313 3781 4069 4138
Size: 76 246 222 47 55 468 288 69
Cuts at: 4138 4309 4330 4336 4381 4510 4677
Size: 171 21 6 45 129 167
Fragments arranged by size:
527 499 468 401 365 308 288 279
246 222 171 167 157 131 129 76
69 55 47 45 21 6
Bpu10I CC'TnA_GC
Cuts at: 0 2651 2817 3077 3655 4101 4449 4463 4664
Size: 2651 166 260 578 446 348 14 201
Cuts at: 4664 4677
Size: 13
Fragments arranged by size:
2651 578 446 348 260 201 166 14
13
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3022 3046 3250 3729 4677
Size: 3022 24 204 479 948
Fragments arranged by size:
3022 948 479 204 24
BsaI GGTCTCn'nnnn_
Cuts at: 0 68 3757 4677
Size: 68 3689 920
Fragments arranged by size:
3689 920 68
BsaAI yAC'GTr
Cuts at: 0 1506 2135 4677
Size: 1506 629 2542
Fragments arranged by size:
2542 1506 629
BsaBI GATnn'nnATC
Cuts at: 0 2008 4677
Size: 2008 2669
BsaHI Gr'CG_yC
Cuts at: 0 1875 2031 3750 4677
Size: 1875 156 1719 927
Fragments arranged by size:
1875 1719 927 156
BsaWI w'CCGG_w
Cuts at: 0 33 654 1904 4677
Size: 33 621 1250 2773
Fragments arranged by size:
2773 1250 621 33
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 25 1252 1282 1760 1790 3352 3382 4049
Size: 25 1227 30 478 30 1562 30 667
Cuts at: 4049 4079 4111 4141 4611 4641 4677
Size: 30 32 30 470 30 36
Fragments arranged by size:
1562 1227 667 478 470 36 32 30
30 30 30 30 30 25
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 282 562 835 898 1055 1408 1411 1945
Size: 282 280 273 63 157 353 3 534
Cuts at: 1945 2962 3208 3531 3643 3976 4108 4210 4435
Size: 1017 246 323 112 333 132 102 225
Cuts at: 4435 4480 4492 4507 4510 4677
Size: 45 12 15 3 167
Fragments arranged by size:
1017 534 353 333 323 282 280 273
246 225 167 157 132 112 102 63
45 15 12 3 3
BseYI C'CCAG_C
Cuts at: 0 59 413 1870 2727 2750 2942 3363 3393
Size: 59 354 1457 857 23 192 421 30
Cuts at: 3393 3580 4410 4546 4677
Size: 187 830 136 131
Fragments arranged by size:
1457 857 830 421 354 192 187 136
131 59 30 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 619 894 1672 1784 3332 4505 4677
Size: 619 275 778 112 1548 1173 172
Fragments arranged by size:
1548 1173 778 619 275 172 112
BsiHKAI G_wGCw'C
Cuts at: 0 1892 2218 2484 2555 3535 3594 3929 4202
Size: 1892 326 266 71 980 59 335 273
Cuts at: 4202 4450 4677
Size: 248 227
Fragments arranged by size:
1892 980 335 326 273 266 248 227
71 59
Bsp1286I G_dGCh'C
Cuts at: 0 43 292 405 1102 1247 1496 1610 1892
Size: 43 249 113 697 145 249 114 282
Cuts at: 1892 2218 2385 2484 2555 2833 3535 3594 3929
Size: 326 167 99 71 278 702 59 335
Cuts at: 3929 4102 4188 4202 4347 4359 4450 4516 4677
Size: 173 86 14 145 12 91 66 161
Fragments arranged by size:
702 697 335 326 282 278 249 249
173 167 161 145 145 114 113 99
91 86 71 66 59 43 14 12
BspEI T'CCGG_A
Cuts at: 0 654 1904 4677
Size: 654 1250 2773
Fragments arranged by size:
2773 1250 654
BspHI T'CATG_A
Cuts at: 0 1706 4202 4677
Size: 1706 2496 475
Fragments arranged by size:
2496 1706 475
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2243 2591 2757 3050 3561 4226 4677
Size: 2243 348 166 293 511 665 451
Fragments arranged by size:
2243 665 511 451 348 293 166
BsrBI CCG'CTC
Cuts at: 0 2028 2406 4677
Size: 2028 378 2271
Fragments arranged by size:
2271 2028 378
BsrDI GCAATG_nn'
Cuts at: 0 954 2930 4677
Size: 954 1976 1747
Fragments arranged by size:
1976 1747 954
BsrFI r'CCGG_y
Cuts at: 0 626 1434 1898 2225 3319 3344 4578 4677
Size: 626 808 464 327 1094 25 1234 99
Fragments arranged by size:
1234 1094 808 626 464 327 99 25
BsrGI T'GTAC_A
Cuts at: 0 3371 4677
Size: 3371 1306
BssSI C'ACGA_G
Cuts at: 0 2657 4677
Size: 2657 2020
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1242 2235 2922 3798 3988 4263 4677
Size: 1242 993 687 876 190 275 414
Fragments arranged by size:
1242 993 876 687 414 275 190
BstEII G'GTnAC_C
Cuts at: 0 3121 4227 4677
Size: 3121 1106 450
Fragments arranged by size:
3121 1106 450
BstXI CCAn_nnnn'nTGG
Cuts at: 0 732 810 2509 3519 4677
Size: 732 78 1699 1010 1158
Fragments arranged by size:
1699 1158 1010 732 78
BstYI r'GATC_y
Cuts at: 0 134 323 707 1076 3110 4113 4295 4677
Size: 134 189 384 369 2034 1003 182 382
Fragments arranged by size:
2034 1003 384 382 369 189 182 134
BstZ17I GTA'TAC
Cuts at: 0 2249 4677
Size: 2249 2428
Bsu36I CC'TnA_GG
Cuts at: 0 2744 3415 3959 4031 4172 4677
Size: 2744 671 544 72 141 505
Fragments arranged by size:
2744 671 544 505 141 72
BtgI C'CryG_G
Cuts at: 0 146 1731 3624 4677
Size: 146 1585 1893 1053
Fragments arranged by size:
1893 1585 1053 146
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 75 93 839 2796 4418 4677
Size: 75 18 746 1957 1622 259
Fragments arranged by size:
1957 1622 746 259 75 18
BtsI GCAGTG_nn'
Cuts at: 0 1850 2674 3604 3688 3775 3838 4020 4677
Size: 1850 824 930 84 87 63 182 657
Fragments arranged by size:
1850 930 824 657 182 87 84 63
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1236 1271 4677
Size: 1236 35 3406
Fragments arranged by size:
3406 1236 35
DraI TTT'AAA
Cuts at: 0 1209 4677
Size: 1209 3468
DraIII CAC_nnn'GTG
Cuts at: 0 2523 4677
Size: 2523 2154
DrdI GACnn_nn'nnGTC
Cuts at: 0 2179 4677
Size: 2179 2498
EaeI y'GGCC_r
Cuts at: 0 624 749 1196 2499 3503 4677
Size: 624 125 447 1303 1004 1174
Fragments arranged by size:
1303 1174 1004 624 447 125
EarI CTCTTCn'nnn_
Cuts at: 0 1385 2018 4478 4677
Size: 1385 633 2460 199
Fragments arranged by size:
2460 1385 633 199
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 127 336 1132 2370 3919 4392 4597 4677
Size: 127 209 796 1238 1549 473 205 80
Fragments arranged by size:
1549 1238 796 473 209 205 127 80
EcoICRI GAG'CTC
Cuts at: 0 2482 2553 3592 3927 4200 4448 4677
Size: 2482 71 1039 335 273 248 229
Fragments arranged by size:
2482 1039 335 273 248 229 71
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 171 527 892 1293 1572 2071 2202 2305
Size: 171 356 365 401 279 499 131 103
Cuts at: 2305 2359 2414 2667 2743 2989 3211 3258 3313
Size: 54 55 253 76 246 222 47 55
Cuts at: 3313 3781 3911 4069 4138 4309 4330 4336 4381
Size: 468 130 158 69 171 21 6 45
Cuts at: 4381 4510 4580 4677
Size: 129 70 97
Fragments arranged by size:
499 468 401 365 356 279 253 246
222 171 171 158 131 130 129 103
97 76 70 69 55 55 54 47
45 21 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2455 3610 4006 4222 4677
Size: 2455 1155 396 216 455
Fragments arranged by size:
2455 1155 455 396 216
EcoO109I rG'GnC_Cy
Cuts at: 0 721 787 2043 2664 3153 3174 3411 3420
Size: 721 66 1256 621 489 21 237 9
Cuts at: 3420 3825 4185 4215 4356 4512 4513 4600 4677
Size: 405 360 30 141 156 1 87 77
Fragments arranged by size:
1256 721 621 489 405 360 237 156
141 87 77 66 30 21 9 1
EcoRV GAT'ATC
Cuts at: 0 1476 4677
Size: 1476 3201
FspI TGC'GCA
Cuts at: 0 3407 4677
Size: 3407 1270
HaeII r_GCGC'y
Cuts at: 0 420 1878 2112 3753 4677
Size: 420 1458 234 1641 924
Fragments arranged by size:
1641 1458 924 420 234
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 27 838 870 972 1004 1310 1342 1760
Size: 27 811 32 102 32 306 32 418
Cuts at: 1760 1792 1901 1933 2843 2875 3307 3339 3412
Size: 32 109 32 910 32 432 32 73
Cuts at: 3412 3444 3657 3689 4111 4143 4677
Size: 32 213 32 422 32 534
Fragments arranged by size:
910 811 534 432 422 418 306 213
109 102 73 32 32 32 32 32
32 32 32 32 32 27
HincII GTy'rAC
Cuts at: 0 851 1842 4148 4677
Size: 851 991 2306 529
Fragments arranged by size:
2306 991 851 529
KasI G'GCGC_C
Cuts at: 0 1874 3749 4677
Size: 1874 1875 928
Fragments arranged by size:
1875 1874 928
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 38 1396 1421 2690 4142 4677
Size: 38 1358 25 1269 1452 535
Fragments arranged by size:
1452 1358 1269 535 38 25
MscI TGG'CCA
Cuts at: 0 751 4677
Size: 751 3926
MslI CAynn'nnrTG
Cuts at: 0 36 808 916 947 1231 1466 1489 1580
Size: 36 772 108 31 284 235 23 91
Cuts at: 1580 1705 1723 2014 2198 4677
Size: 125 18 291 184 2479
Fragments arranged by size:
2479 772 291 284 235 184 125 108
91 36 31 23 18
MspA1I CmG'CkG
Cuts at: 0 148 1349 1805 1990 2498 2727 2763 3486
Size: 148 1201 456 185 508 229 36 723
Cuts at: 3486 3857 4260 4677
Size: 371 403 417
Fragments arranged by size:
1201 723 508 456 417 403 371 229
185 148 36
NaeI GCC'GGC
Cuts at: 0 628 1900 2227 3321 4677
Size: 628 1272 327 1094 1356
Fragments arranged by size:
1356 1272 1094 628 327
NarI GG'CG_CC
Cuts at: 0 1875 3750 4677
Size: 1875 1875 927
Fragments arranged by size:
1875 1875 927
NcoI C'CATG_G
Cuts at: 0 1731 3624 4677
Size: 1731 1893 1053
Fragments arranged by size:
1893 1731 1053
NdeI CA'TA_TG
Cuts at: 0 969 1238 4252 4677
Size: 969 269 3014 425
Fragments arranged by size:
3014 969 425 269
NgoMIV G'CCGG_C
Cuts at: 0 626 1898 2225 3319 4677
Size: 626 1272 327 1094 1358
Fragments arranged by size:
1358 1272 1094 626 327
NruI TCG'CGA
Cuts at: 0 94 4677
Size: 94 4583
NspI r_CATG'y
Cuts at: 0 1230 4677
Size: 1230 3447
PflMI CCAn_nnn'nTGG
Cuts at: 0 275 1415 2523 3857 4677
Size: 275 1140 1108 1334 820
Fragments arranged by size:
1334 1140 1108 820 275
PfoI T'CCnGG_A
Cuts at: 0 869 3235 4472 4677
Size: 869 2366 1237 205
Fragments arranged by size:
2366 1237 869 205
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1252 1284 4677
Size: 1252 32 3393
Fragments arranged by size:
3393 1252 32
PpuMI rG'GwC_Cy
Cuts at: 0 3174 3411 3420 4215 4677
Size: 3174 237 9 795 462
Fragments arranged by size:
3174 795 462 237 9
PshAI GACnn'nnGTC
Cuts at: 0 3608 4677
Size: 3608 1069
PspOMI G'GGCC_C
Cuts at: 0 4184 4355 4512 4677
Size: 4184 171 157 165
Fragments arranged by size:
4184 171 165 157
PspXI vC'TCGA_Gb
Cuts at: 0 4222 4677
Size: 4222 455
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1436 1468 4677
Size: 1436 32 3209
Fragments arranged by size:
3209 1436 32
PstI C_TGCA'G
Cuts at: 0 647 1181 2435 2702 2918 3485 3599 3794
Size: 647 534 1254 267 216 567 114 195
Cuts at: 3794 3833 3984 4268 4677
Size: 39 151 284 409
Fragments arranged by size:
1254 647 567 534 409 284 267 216
195 151 114 39
PvuII CAG'CTG
Cuts at: 0 1990 2727 3486 3857 4260 4677
Size: 1990 737 759 371 403 417
Fragments arranged by size:
1990 759 737 417 403 371
SacI G_AGCT'C
Cuts at: 0 2484 2555 3594 3929 4202 4450 4677
Size: 2484 71 1039 335 273 248 227
Fragments arranged by size:
2484 1039 335 273 248 227 71
SacII CC_GC'GG
Cuts at: 0 149 4677
Size: 149 4528
SanDI GG'GwC_CC
Cuts at: 0 3420 4677
Size: 3420 1257
SapI GCTCTTCn'nnn_
Cuts at: 0 2018 4478 4677
Size: 2018 2460 199
Fragments arranged by size:
2460 2018 199
SexAI A'CCwGG_T
Cuts at: 0 1594 4677
Size: 1594 3083
SfcI C'TryA_G
Cuts at: 0 336 643 703 1177 2431 2533 2698 2914
Size: 336 307 60 474 1254 102 165 216
Cuts at: 2914 3214 3481 3595 3790 3829 3940 3980 4264
Size: 300 267 114 195 39 111 40 284
Cuts at: 4264 4677
Size: 413
Fragments arranged by size:
1254 474 413 336 307 300 284 267
216 195 165 114 111 102 60 40
39
SfoI GGC'GCC
Cuts at: 0 1876 3751 4677
Size: 1876 1875 926
Fragments arranged by size:
1876 1875 926
SmaI CCC'GGG
Cuts at: 0 1165 3085 4183 4677
Size: 1165 1920 1098 494
Fragments arranged by size:
1920 1165 1098 494
SmlI C'TyrA_G
Cuts at: 0 989 3001 3061 3265 3744 4222 4677
Size: 989 2012 60 204 479 478 455
Fragments arranged by size:
2012 989 479 478 455 204 60
SrfI GCCC'GGGC
Cuts at: 0 3085 4677
Size: 3085 1592
SspI AAT'ATT
Cuts at: 0 444 4677
Size: 444 4233
StuI AGG'CCT
Cuts at: 0 2641 3250 4677
Size: 2641 609 1427
Fragments arranged by size:
2641 1427 609
StyI C'CwwG_G
Cuts at: 0 1082 1731 1815 2252 2834 3624 4190 4677
Size: 1082 649 84 437 582 790 566 487
Fragments arranged by size:
1082 790 649 582 566 487 437 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 4539 4567 4677
Size: 4539 28 110
Fragments arranged by size:
4539 110 28
TatI w'GTAC_w
Cuts at: 0 1452 3371 4677
Size: 1452 1919 1306
Fragments arranged by size:
1919 1452 1306
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 382 414 4049 4081 4611 4643 4677
Size: 382 32 3635 32 530 32 34
Fragments arranged by size:
3635 530 382 34 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 149 1254 2524 3695 3879 4677
Size: 149 1105 1270 1171 184 798
Fragments arranged by size:
1270 1171 1105 798 184 149
XhoI C'TCGA_G
Cuts at: 0 989 4222 4677
Size: 989 3233 455
Fragments arranged by size:
3233 989 455
XmaI C'CCGG_G
Cuts at: 0 1163 3083 4181 4677
Size: 1163 1920 1098 496
Fragments arranged by size:
1920 1163 1098 496
XmnI GAAnn'nnTTC
Cuts at: 0 1267 4677
Size: 1267 3410
Enzymes that do cut:
AarI AccI AcuI AflIII AhdI AleI AlfI AloI
AlwNI ApaI ApoI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI CspCI DraI DraIII DrdI EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FspI HaeII
Hin4I HincII KasI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NruI NspI PflMI PfoI
PpiI PpuMI PshAI PspOMI PspXI PsrI PstI PvuII
SacI SacII SanDI SapI SexAI SfcI SfoI SmaI
SmlI SrfI SspI StuI StyI TaqII TatI TstI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI ApaLI AscI
AseI AsiSI AvrII BaeI BclI BfrBI BmtI BsiEI
BsiWI BsmI BsmBI BssHII BstBI ClaI EagI EcoRI
FalI FseI FspAI HindIII HpaI KpnI MfeI MluI
NheI NotI NsiI PacI PasI PciI PmeI PmlI
PsiI PvuI RsrII SalI SbfI ScaI SfiI SgfI
SgrAI SnaBI SpeI SphI SwaI Tth111I XbaI ZraI