Restriction map for pF1KE9652
 (Linear) (Six-base) MAPSORT of: KIEE9652.seq  Check: 7830  from: 1  to: 7743



KIEE9652.orf

 With 182 enzymes: SgfI * 

                        July 16, 2019 14:21  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1017    3380    7743 
   Size:       1017    2363    4363

  Fragments arranged by size: 

                4363    2363    1017
 
AccI GT'mk_AC

Cuts at:      0     927    1931    5431    5965    7457    7743 
   Size:        927    1004    3500     534    1492     286

  Fragments arranged by size: 

                3500    1492    1004     927     534     286
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     826    2009    3332    3567    4055    7743 
   Size:        826    1183    1323     235     488    3688

  Fragments arranged by size: 

                3688    1323    1183     826     488     235
 
AfeI AGC'GCT

Cuts at:      0    2098    7743 
   Size:       2098    5645
 
AflII C'TTAA_G

Cuts at:      0     484    3073    7743 
   Size:        484    2589    4670

  Fragments arranged by size: 

                4670    2589     484
 
AflIII A'CryG_T

Cuts at:      0    7334    7743 
   Size:       7334     409
 
AhdI GACnn_n'nnGTC

Cuts at:      0    4445    7743 
   Size:       4445    3298
 
AleI CACnn'nnGTG

Cuts at:      0    2659    4481    7388    7743 
   Size:       2659    1822    2907     355

  Fragments arranged by size: 

                2907    2659    1822     355
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2396    5863    6604    7743 
   Size:       2396    3467     741    1139

  Fragments arranged by size: 

                3467    2396    1139     741
 
AlwNI CAG_nnn'CTG

Cuts at:      0     511     567     772    3661    5590    7743 
   Size:        511      56     205    2889    1929    2153

  Fragments arranged by size: 

                2889    2153    1929     511     205      56
 
ApaI G_GGCC'C

Cuts at:      0     800    7257    7743 
   Size:        800    6457     486

  Fragments arranged by size: 

                6457     800     486
 
ApaLI G'TGCA_C

Cuts at:      0    7436    7743 
   Size:       7436     307
 
ApoI r'AATT_y

Cuts at:      0      36     177     530    1999    2320    2629    3201    3331 
   Size:         36     141     353    1469     321     309     572     130

Cuts at:   3331    4252    6566    7743 
   Size:        921    2314    1177

  Fragments arranged by size: 

                2314    1469    1177     921     572     353     321     309
                 141     130      36
 
AseI AT'TA_AT

Cuts at:      0    3647    7743 
   Size:       3647    4096
 
AvaI C'yCGr_G

Cuts at:      0    1442    1688    3626    6025    7743 
   Size:       1442     246    1938    2399    1718

  Fragments arranged by size: 

                2399    1938    1718    1442     246
 
BamHI G'GATC_C

Cuts at:      0    4652    6941    7743 
   Size:       4652    2289     802

  Fragments arranged by size: 

                4652    2289     802
 
BanI G'GyrC_C

Cuts at:      0     514    1567    2342    3123    7743 
   Size:        514    1053     775     781    4620

  Fragments arranged by size: 

                4620    1053     781     775     514
 
BanII G_rGCy'C

Cuts at:      0     800    1247    1416    1970    2153    2228    5227    5630 
   Size:        800     447     169     554     183      75    2999     403

Cuts at:   5630    7257    7539    7743 
   Size:       1627     282     204

  Fragments arranged by size: 

                2999    1627     800     554     447     403     282     204
                 183     169      75
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    3099    3603    4462    7743 
   Size:       3099     504     859    3281

  Fragments arranged by size: 

                3281    3099     859     504
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2072    6144    7743 
   Size:       2072    4072    1599

  Fragments arranged by size: 

                4072    2072    1599
 
BclI T'GATC_A

Cuts at:      0    2768    7743 
   Size:       2768    4975
 
BglI GCCn_nnn'nGGC

Cuts at:      0     523    4138    5781    7743 
   Size:        523    3615    1643    1962

  Fragments arranged by size: 

                3615    1962    1643     523
 
BglII A'GATC_T

Cuts at:      0    3912    3995    5156    7743 
   Size:       3912      83    1161    2587

  Fragments arranged by size: 

                3912    2587    1161      83
 
BlpI GC'TnA_GC

Cuts at:      0     560    7743 
   Size:        560    7183
 
Bme1580I G_kGCm'C

Cuts at:      0     519     800    1422    7257    7440    7743 
   Size:        519     281     622    5835     183     303

  Fragments arranged by size: 

                5835     622     519     303     281     183
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1901    2628    4831    6044    6705    7322    7743 
   Size:       1901     727    2203    1213     661     617     421

  Fragments arranged by size: 

                2203    1901    1213     727     661     617     421
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    5175    5664    7743 
   Size:       5175     489    2079

  Fragments arranged by size: 

                5175    2079     489
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     236     242     417     464     733     856     908    4873 
   Size:        236       6     175      47     269     123      52    3965

Cuts at:   4873    5612    6076    6208    6255    6261    6617    6635    6907 
   Size:        739     464     132      47       6     356      18     272

Cuts at:   6907    6952    7030    7446    7707    7743 
   Size:         45      78     416     261      36

  Fragments arranged by size: 

                3965     739     464     416     356     272     269     261
                 236     175     132     123      78      52      47      47
                  45      36      18       6       6
 
Bpu10I CC'TnA_GC

Cuts at:      0     385    1829    2804    4058    6419    7743 
   Size:        385    1444     975    1254    2361    1324

  Fragments arranged by size: 

                2361    1444    1324    1254     975     385
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     959    2875    3001    3019    3802    6243    7551    7743 
   Size:        959    1916     126      18     783    2441    1308     192

  Fragments arranged by size: 

                2441    1916    1308     959     783     192     126      18
            
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    4432    4772    6928    7322    7743 
   Size:       4432     340    2156     394     421

  Fragments arranged by size: 

                4432    2156     421     394     340
 
BsaAI yAC'GTr

Cuts at:      0    2169    7743 
   Size:       2169    5574
 
BsaBI GATnn'nnATC

Cuts at:      0     478    5417    6836    7743 
   Size:        478    4939    1419     907

  Fragments arranged by size: 

                4939    1419     907     478
 
BsaHI Gr'CG_yC

Cuts at:      0     340    2733    5250    7743 
   Size:        340    2393    2517    2493

  Fragments arranged by size: 

                2517    2493    2393     340
 
BsaWI w'CCGG_w

Cuts at:      0    4655    7743 
   Size:       4655    3088
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1593    1623    6880    6910    7003    7033    7705    7735 
   Size:       1593      30    5257      30      93      30     672      30

Cuts at:   7735    7743 
   Size:          8

  Fragments arranged by size: 

                5257    1593     672      93      30      30      30      30
                   8
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     420     492    1301    1498    1552    3528    5167    5653 
   Size:        420      72     809     197      54    1976    1639     486

Cuts at:   5653    6043    6691    7624    7743 
   Size:        390     648     933     119

  Fragments arranged by size: 

                1976    1639     933     809     648     486     420     390
                 197     119      72      54
 
BseYI C'CCAG_C

Cuts at:      0     721    1091    1109    1468    1519    4527    4953    5583 
   Size:        721     370      18     359      51    3008     426     630

Cuts at:   5583    7743 
   Size:       2160

  Fragments arranged by size: 

                3008    2160     721     630     426     370     359      51
                  18
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1051    1661    5938    6680    7743 
   Size:       1051     610    4277     742    1063

  Fragments arranged by size: 

                4277    1063    1051     742     610
 
BsiHKAI G_wGCw'C

Cuts at:      0    1970    2257    5916    6083    7440    7743 
   Size:       1970     287    3659     167    1357     303

  Fragments arranged by size: 

                3659    1970    1357     303     287     167
 
BsmI GAATG_Cn'

Cuts at:      0     990    1709    4751    7743 
   Size:        990     719    3042    2992

  Fragments arranged by size: 

                3042    2992     990     719
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1713    7743 
   Size:       1713    6030
 
Bsp1286I G_dGCh'C

Cuts at:      0     519     800    1247    1416    1422    1970    2153    2228 
   Size:        519     281     447     169       6     548     183      75

Cuts at:   2228    2257    5227    5630    5916    6083    7257    7440    7539 
   Size:         29    2970     403     286     167    1174     183      99

Cuts at:   7539    7743 
   Size:        204

  Fragments arranged by size: 

                2970    1174     548     519     447     403     286     281
                 204     183     183     169     167      99      75      29
                   6
 
BspEI T'CCGG_A

Cuts at:      0    4655    7743 
   Size:       4655    3088
 
BspHI T'CATG_A

Cuts at:      0    3360    7743 
   Size:       3360    4383
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1017    3217    3380    3676    3716    7743 
   Size:       1017    2200     163     296      40    4027

  Fragments arranged by size: 

                4027    2200    1017     296     163      40
 
BsrBI CCG'CTC

Cuts at:      0    4284    6332    7743 
   Size:       4284    2048    1411

  Fragments arranged by size: 

                4284    2048    1411
 
BsrDI GCAATG_nn'

Cuts at:      0    1403    2537    2591    2935    7743 
   Size:       1403    1134      54     344    4808

  Fragments arranged by size: 

                4808    1403    1134     344      54
 
BsrFI r'CCGG_y

Cuts at:      0     619    3394    5321    7743 
   Size:        619    2775    1927    2422

  Fragments arranged by size: 

                2775    2422    1927     619
 
BsrGI T'GTAC_A

Cuts at:      0    1131    7743 
   Size:       1131    6612
 
BssSI C'ACGA_G

Cuts at:      0    4459    4589    6082    7743 
   Size:       4459     130    1493    1661

  Fragments arranged by size: 

                4459    1661    1493     130
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     915    1306    3281    4334    7743 
   Size:        915     391    1975    1053    3409

  Fragments arranged by size: 

                3409    1975    1053     915     391
 
BstEII G'GTnAC_C

Cuts at:      0    5187    7743 
   Size:       5187    2556
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     157    1278    4868    7743 
   Size:        157    1121    3590    2875

  Fragments arranged by size: 

                3590    2875    1121     157
 
BstYI r'GATC_y

Cuts at:      0      18     380    3030    3912    3995    4155    4652    5076 
   Size:         18     362    2650     882      83     160     497     424

Cuts at:   5076    5156    6941    7743 
   Size:         80    1785     802

  Fragments arranged by size: 

                2650    1785     882     802     497     424     362     160
                  83      80      18
 
BstZ17I GTA'TAC

Cuts at:      0    5966    7743 
   Size:       5966    1777
 
Bsu36I CC'TnA_GG

Cuts at:      0    4759    7607    7743 
   Size:       4759    2848     136

  Fragments arranged by size: 

                4759    2848     136
 
BtgI C'CryG_G

Cuts at:      0    1422    2574    2667    3380    4482    5541    5817    6668 
   Size:       1422    1152      93     713    1102    1059     276     851

Cuts at:   6668    7743 
   Size:       1075

  Fragments arranged by size: 

                1422    1152    1102    1075    1059     851     713     276
                  93
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1061    7743 
   Size:       1061    6682
 
BtsI GCAGTG_nn'

Cuts at:      0    5947    5975    5986    7395    7597    7743 
   Size:       5947      28      11    1409     202     146

  Fragments arranged by size: 

                5947    1409     202     146      28      11
 
DraI TTT'AAA

Cuts at:      0    2325    4914    6071    7743 
   Size:       2325    2589    1157    1672

  Fragments arranged by size: 

                2589    2325    1672    1157
 
DraIII CAC_nnn'GTG

Cuts at:      0    2916    6087    7743 
   Size:       2916    3171    1656

  Fragments arranged by size: 

                3171    2916    1656
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    3607    4307    6634    7743 
   Size:       3607     700    2327    1109

  Fragments arranged by size: 

                3607    2327    1109     700
 
EaeI y'GGCC_r

Cuts at:      0     671    1477    2678    3444    4285    4980    5552    6092 
   Size:        671     806    1201     766     841     695     572     540

Cuts at:   6092    7743 
   Size:       1651

  Fragments arranged by size: 

                1651    1201     841     806     766     695     671     572
                 540
 
EarI CTCTTCn'nnn_

Cuts at:      0    1227    1242    1507    1546    1621    1711    1793    2141 
   Size:       1227      15     265      39      75      90      82     348

Cuts at:   2141    3974    6302    6344    6899    7557    7743 
   Size:       1833    2328      42     555     658     186

  Fragments arranged by size: 

                2328    1833    1227     658     555     348     265     186
                  90      82      75      42      39      15
 
EcoICRI GAG'CTC

Cuts at:      0    1968    7743 
   Size:       1968    5775
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     236     242     417     464     733     826     856     908 
   Size:        236       6     175      47     269      93      30      52

Cuts at:    908    2009    3332    3567    4055    4873    5612    6076    6208 
   Size:       1101    1323     235     488     818     739     464     132

Cuts at:   6208    6255    6261    6617    6635    6907    6952    7030    7446 
   Size:         47       6     356      18     272      45      78     416

Cuts at:   7446    7707    7743 
   Size:        261      36

  Fragments arranged by size: 

                1323    1101     818     739     488     464     416     356
                 272     269     261     236     235     175     132      93
                  78      52      47      47      45      36      30      18
                   6       6
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    3562    4762    4972    7297    7743 
   Size:       3562    1200     210    2325     446

  Fragments arranged by size: 

                3562    2325    1200     446     210
 
EcoO109I rG'GnC_Cy

Cuts at:      0     489     681     796     797     892    1232    1761    5101 
   Size:        489     192     115       1      95     340     529    3340

Cuts at:   5101    5853    6045    7222    7743 
   Size:        752     192    1177     521

  Fragments arranged by size: 

                3340    1177     752     529     521     489     340     192
                 192     115      95       1
 
EcoRI G'AATT_C

Cuts at:      0     177    1999    3331    4252    7743 
   Size:        177    1822    1332     921    3491

  Fragments arranged by size: 

                3491    1822    1332     921     177
 
EcoRV GAT'ATC

Cuts at:      0    4635    7743 
   Size:       4635    3108
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    4083    4722    7743 
   Size:       4083     639    3021

  Fragments arranged by size: 

                4083    3021     639
 
FspI TGC'GCA

Cuts at:      0    3274    7743 
   Size:       3274    4469
 
HaeII r_GCGC'y

Cuts at:      0    2100    5685    7743 
   Size:       2100    3585    2058

  Fragments arranged by size: 

                3585    2100    2058
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    2067    2099    2723    2755    3450    3482    3897    3929 
   Size:       2067      32     624      32     695      32     415      32

Cuts at:   3929    3949    3981    4378    4410    4431    4463    5017    5049 
   Size:         20      32     397      32      21      32     554      32

Cuts at:   5049    6193    6225    6586    6618    6670    6702    7633    7665 
   Size:       1144      32     361      32      52      32     931      32

Cuts at:   7665    7743 
   Size:         78

  Fragments arranged by size: 

                2067    1144     931     695     624     554     415     397
                 361      78      52      32      32      32      32      32
                  32      32      32      32      32      32      32      21
                  20
 
HincII GTy'rAC

Cuts at:      0    5055    6788    7743 
   Size:       5055    1733     955

  Fragments arranged by size: 

                5055    1733     955
 
HindIII A'AGCT_T

Cuts at:      0    3341    3430    4065    7743 
   Size:       3341      89     635    3678

  Fragments arranged by size: 

                3678    3341     635      89
 
HpaI GTT'AAC

Cuts at:      0    6788    7743 
   Size:       6788     955
 
MfeI C'AATT_G

Cuts at:      0    5000    7743 
   Size:       5000    2743
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    2426    3809    3909    4885    5003    7365    7743 
   Size:       2426    1383     100     976     118    2362     378

  Fragments arranged by size: 

                2426    2362    1383     976     378     118     100
 
MscI TGG'CCA

Cuts at:      0    2680    3446    5554    6094    7743 
   Size:       2680     766    2108     540    1649

  Fragments arranged by size: 

                2680    2108    1649     766     540
 
MslI CAynn'nnrTG

Cuts at:      0     908    2141    2659    3262    4319    4459    4481    4922 
   Size:        908    1233     518     603    1057     140      22     441

Cuts at:   4922    6920    7388    7743 
   Size:       1998     468     355

  Fragments arranged by size: 

                1998    1233    1057     908     603     518     468     441
                 355     140      22
 
MspA1I CmG'CkG

Cuts at:      0     424     741     960    1023    1424    3658    4531    5590 
   Size:        424     317     219      63     401    2234     873    1059

Cuts at:   5590    6703    7743 
   Size:       1113    1040

  Fragments arranged by size: 

                2234    1113    1059    1040     873     424     401     317
                 219      63
 
NaeI GCC'GGC

Cuts at:      0    3396    7743 
   Size:       3396    4347
 
NcoI C'CATG_G

Cuts at:      0    2574    2667    3380    5541    5817    6668    7743 
   Size:       2574      93     713    2161     276     851    1075

  Fragments arranged by size: 

                2574    2161    1075     851     713     276      93
 
NdeI CA'TA_TG

Cuts at:      0    3933    4828    7743 
   Size:       3933     895    2915

  Fragments arranged by size: 

                3933    2915     895
 
NgoMIV G'CCGG_C

Cuts at:      0    3394    7743 
   Size:       3394    4349
 
NspI r_CATG'y

Cuts at:      0     913    3243    7743 
   Size:        913    2330    4500

  Fragments arranged by size: 

                4500    2330     913
 
PasI CC'CwG_GG

Cuts at:      0     685     784     840    7743 
   Size:        685      99      56    6903

  Fragments arranged by size: 

                6903     685      99      56
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     886    1216    4118    5590    5768    6552    7743 
   Size:        886     330    2902    1472     178     784    1191

  Fragments arranged by size: 

                2902    1472    1191     886     784     330     178
 
PfoI T'CCnGG_A

Cuts at:      0    6163    6612    7743 
   Size:       6163     449    1131

  Fragments arranged by size: 

                6163    1131     449
 
PpuMI rG'GwC_Cy

Cuts at:      0     681     892    1761    5101    5853    6045    7743 
   Size:        681     211     869    3340     752     192    1698

  Fragments arranged by size: 

                3340    1698     869     752     681     211     192
 
PsiI TTA'TAA

Cuts at:      0    4697    7743 
   Size:       4697    3046
 
PspOMI G'GGCC_C

Cuts at:      0     796    7253    7743 
   Size:        796    6457     490

  Fragments arranged by size: 

                6457     796     490
 
PspXI vC'TCGA_Gb

Cuts at:      0    1442    7743 
   Size:       1442    6301
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1244    1276    7743 
   Size:       1244      32    6467

  Fragments arranged by size: 

                6467    1244      32
 
PstI C_TGCA'G

Cuts at:      0     816    1028    1208    3377    3713    5310    6437    6693 
   Size:        816     212     180    2169     336    1597    1127     256

Cuts at:   6693    7743 
   Size:       1050

  Fragments arranged by size: 

                2169    1597    1127    1050     816     336     256     212
                 180
 
PvuII CAG'CTG

Cuts at:      0     424     741     960    1023    3658    4531    5590    7743 
   Size:        424     317     219      63    2635     873    1059    2153

  Fragments arranged by size: 

                2635    2153    1059     873     424     317     219      63
            
 
SacI G_AGCT'C

Cuts at:      0    1970    7743 
   Size:       1970    5773
 
SacII CC_GC'GG

Cuts at:      0    1425    7743 
   Size:       1425    6318
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1227    2141    6899    7743 
   Size:       1227     914    4758     844

  Fragments arranged by size: 

                4758    1227     914     844
 
SbfI CC_TGCA'GG

Cuts at:      0    3377    3713    7743 
   Size:       3377     336    4030

  Fragments arranged by size: 

                4030    3377     336
 
ScaI AGT'ACT

Cuts at:      0    5536    7743 
   Size:       5536    2207
 
SexAI A'CCwGG_T

Cuts at:      0      77     636    2240    4244    7743 
   Size:         77     559    1604    2004    3499

  Fragments arranged by size: 

                3499    2004    1604     559      77
 
SfcI C'TryA_G

Cuts at:      0     812    1024    1204    1250    1613    2948    3061    3373 
   Size:        812     212     180      46     363    1335     113     312

Cuts at:   3373    3709    5306    6433    6689    7360    7743 
   Size:        336    1597    1127     256     671     383

  Fragments arranged by size: 

                1597    1335    1127     812     671     383     363     336
                 312     256     212     180     113      46
 
SmaI CCC'GGG

Cuts at:      0    3628    7743 
   Size:       3628    4115
 
SmlI C'TyrA_G

Cuts at:      0     484     974    1442    1688    2890    3016    3034    3073 
   Size:        484     490     468     246    1202     126      18      39

Cuts at:   3073    3817    6222    7530    7743 
   Size:        744    2405    1308     213

  Fragments arranged by size: 

                2405    1308    1202     744     490     484     468     246
                 213     126      39      18
 
SnaBI TAC'GTA

Cuts at:      0    2169    7743 
   Size:       2169    5574
 
SspI AAT'ATT

Cuts at:      0    4497    7743 
   Size:       4497    3246
 
StyI C'CwwG_G

Cuts at:      0     108     134     390     540     677     897     990    1004 
   Size:        108      26     256     150     137     220      93      14

Cuts at:   1004    1227    2574    2667    3380    5097    5541    5817    6464 
   Size:        223    1347      93     713    1717     444     276     647

Cuts at:   6464    6668    7743 
   Size:        204    1075

  Fragments arranged by size: 

                1717    1347    1075     713     647     444     276     256
                 223     220     204     150     137     108      93      93
                  26      14
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     397    1123    2360    3141    3305    6970    7420    7743 
   Size:        397     726    1237     781     164    3665     450     323

  Fragments arranged by size: 

                3665    1237     781     726     450     397     323     164
            
 
TatI w'GTAC_w

Cuts at:      0    1131    2012    2711    4463    5534    6510    7149    7743 
   Size:       1131     881     699    1752    1071     976     639     594

  Fragments arranged by size: 

                1752    1131    1071     976     881     699     639     594
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1500    1532    1593    1625    5385    5417    6880    6912 
   Size:       1500      32      61      32    3760      32    1463      32

Cuts at:   6912    7743 
   Size:        831

  Fragments arranged by size: 

                3760    1500    1463     831      61      32      32      32
                  32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1122    6496    7743 
   Size:       1122    5374    1247

  Fragments arranged by size: 

                5374    1247    1122
 
XbaI T'CTAG_A

Cuts at:      0    7165    7743 
   Size:       7165     578
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    3819    4868    7743 
   Size:       3819    1049    2875

  Fragments arranged by size: 

                3819    2875    1049
 
XhoI C'TCGA_G

Cuts at:      0    1442    1688    7743 
   Size:       1442     246    6055

  Fragments arranged by size: 

                6055    1442     246
 
XmaI C'CCGG_G

Cuts at:      0    3626    7743 
   Size:       3626    4117
 
XmnI GAAnn'nnTTC

Cuts at:      0     400    3050    3202    4044    5675    7743 
   Size:        400    2650     152     842    1631    2068

  Fragments arranged by size: 

                2650    2068    1631     842     400     152
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AfeI    AflII   AflIII     AhdI     AleI
     AlfI    AlwNI     ApaI    ApaLI     ApoI     AseI     AvaI    BamHI
     BanI    BanII     BbsI    BciVI     BclI     BglI    BglII     BlpI
 Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI    BssSI   BstAPI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI     DraI   DraIII     DrdI     EaeI
     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FspI    HaeII    Hin4I   HincII  HindIII     HpaI     MfeI     MmeI
     MscI     MslI   MspA1I     NaeI     NcoI     NdeI   NgoMIV     NspI
     PasI    PflMI     PfoI    PpuMI     PsiI   PspOMI    PspXI     PsrI
     PstI    PvuII     SacI    SacII     SapI     SbfI     ScaI    SexAI
     SfcI     SmaI     SmlI    SnaBI     SspI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AgeI     AloI     AscI    AsiSI    AvrII
     BaeI     BbeI    BbvCI     BcgI    BfrBI    BmgBI     BmtI    BsiEI
    BsiWI   BssHII    BstBI     ClaI    CspCI     EagI     EciI     FseI
    FspAI     KasI     KpnI     MluI     NarI     NheI     NotI     NruI
     NsiI     PacI     PciI     PmeI     PmlI     PpiI    PshAI     PvuI
    RsrII     SalI    SanDI     SfiI     SfoI     SgfI    SgrAI     SpeI
     SphI     SrfI     StuI     SwaI     ZraI
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