Restriction map for
pF1KE9656
(Linear) (Six-base) MAPSORT of: KIEE9656.seq Check: 3722 from: 1 to: 492
KIEE9656.orf
With 182 enzymes: SgfI *
March 10, 2017 10:38 ..
AccI GT'mk_AC
Cuts at: 0 201 492
Size: 201 291
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 360 492
Size: 360 132
ApaI G_GGCC'C
Cuts at: 0 122 166 492
Size: 122 44 326
Fragments arranged by size:
326 122 44
AvaI C'yCGr_G
Cuts at: 0 166 484 492
Size: 166 318 8
Fragments arranged by size:
318 166 8
BanI G'GyrC_C
Cuts at: 0 32 222 480 492
Size: 32 190 258 12
Fragments arranged by size:
258 190 32 12
BanII G_rGCy'C
Cuts at: 0 122 166 492
Size: 122 44 326
Fragments arranged by size:
326 122 44
BciVI GTATCCnnnnn_n'
Cuts at: 0 235 492
Size: 235 257
Bme1580I G_kGCm'C
Cuts at: 0 122 166 485 492
Size: 122 44 319 7
Fragments arranged by size:
319 122 44 7
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 355 421 492
Size: 355 66 71
Fragments arranged by size:
355 71 66
BsaI GGTCTCn'nnnn_
Cuts at: 0 397 492
Size: 397 95
BsaAI yAC'GTr
Cuts at: 0 246 492
Size: 246 246
BsaHI Gr'CG_yC
Cuts at: 0 68 492
Size: 68 424
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 34 64 200 230 492
Size: 34 30 136 30 262
Fragments arranged by size:
262 136 34 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 226 337 340 492
Size: 226 111 3 152
Fragments arranged by size:
226 152 111 3
BseYI C'CCAG_C
Cuts at: 0 451 492
Size: 451 41
BsiEI CG_ry'CG
Cuts at: 0 351 492
Size: 351 141
Bsp1286I G_dGCh'C
Cuts at: 0 122 166 485 492
Size: 122 44 319 7
Fragments arranged by size:
319 122 44 7
BsrBI CCG'CTC
Cuts at: 0 8 339 492
Size: 8 331 153
Fragments arranged by size:
331 153 8
BsrDI GCAATG_nn'
Cuts at: 0 58 492
Size: 58 434
BssSI C'ACGA_G
Cuts at: 0 235 492
Size: 235 257
BstAPI GCAn_nnn'nTGC
Cuts at: 0 93 492
Size: 93 399
BtsI GCAGTG_nn'
Cuts at: 0 82 365 492
Size: 82 283 127
Fragments arranged by size:
283 127 82
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 414 449 492
Size: 414 35 43
Fragments arranged by size:
414 43 35
DraIII CAC_nnn'GTG
Cuts at: 0 392 492
Size: 392 100
EaeI y'GGCC_r
Cuts at: 0 20 492
Size: 20 472
EarI CTCTTCn'nnn_
Cuts at: 0 254 492
Size: 254 238
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 42 145 492
Size: 42 103 347
Fragments arranged by size:
347 103 42
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 355 360 421 492
Size: 355 5 61 71
Fragments arranged by size:
355 71 61 5
EcoO109I rG'GnC_Cy
Cuts at: 0 162 163 437 492
Size: 162 1 274 55
Fragments arranged by size:
274 162 55 1
HaeII r_GCGC'y
Cuts at: 0 138 492
Size: 138 354
MspA1I CmG'CkG
Cuts at: 0 197 333 492
Size: 197 136 159
Fragments arranged by size:
197 159 136
PfoI T'CCnGG_A
Cuts at: 0 304 492
Size: 304 188
PspOMI G'GGCC_C
Cuts at: 0 118 162 492
Size: 118 44 330
Fragments arranged by size:
330 118 44
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 34 66 164 196 200 232 492
Size: 34 32 98 32 4 32 260
Fragments arranged by size:
260 98 34 32 32 32 4
Enzymes that do cut:
AccI AcuI ApaI AvaI BanI BanII BciVI Bme1580I
BpmI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsiEI
Bsp1286I BsrBI BsrDI BssSI BstAPI BtsI CspCI DraIII
EaeI EarI EciI Eco57MI EcoO109I HaeII MspA1I PfoI
PspOMI TstI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BbsI BbvCI
BcgI BclI BfrBI BglI BglII BlpI BmgBI BmrI
BmtI BplI Bpu10I BpuEI BsaBI BsaWI BsgI BsiHKAI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrFI BsrGI
BssHII BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI ClaI DraI DrdI EagI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
SwaI TaqII TatI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI