Restriction map for
pF1KE9669
(Linear) (Six-base) MAPSORT of: KIEE9669.seq Check: 4908 from: 1 to: 8991
KIEE9669.orf
With 182 enzymes: SgfI *
October 13, 2011 18:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3510 8991
Size: 3510 5481
AatII G_ACGT'C
Cuts at: 0 3567 7597 8991
Size: 3567 4030 1394
Fragments arranged by size:
4030 3567 1394
AccI GT'mk_AC
Cuts at: 0 374 8991
Size: 374 8617
Acc65I G'GTAC_C
Cuts at: 0 453 3989 8991
Size: 453 3536 5002
Fragments arranged by size:
5002 3536 453
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2231 2300 3349 3417 3455 3803 3925 4769
Size: 2231 69 1049 68 38 348 122 844
Cuts at: 4769 5212 5357 5609 6027 6866 7804 8257 8764
Size: 443 145 252 418 839 938 453 507
Cuts at: 8764 8991
Size: 227
Fragments arranged by size:
2231 1049 938 844 839 507 453 443
418 348 252 227 145 122 69 68
38
AfeI AGC'GCT
Cuts at: 0 2272 8991
Size: 2272 6719
AflII C'TTAA_G
Cuts at: 0 6232 8991
Size: 6232 2759
AflIII A'CryG_T
Cuts at: 0 5731 8423 8991
Size: 5731 2692 568
Fragments arranged by size:
5731 2692 568
AhdI GACnn_n'nnGTC
Cuts at: 0 3269 5732 6681 6747 8991
Size: 3269 2463 949 66 2244
Fragments arranged by size:
3269 2463 2244 949 66
AleI CACnn'nnGTG
Cuts at: 0 1106 7057 8991
Size: 1106 5951 1934
Fragments arranged by size:
5951 1934 1106
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3892 6193 8991
Size: 3892 2301 2798
Fragments arranged by size:
3892 2798 2301
AlwNI CAG_nnn'CTG
Cuts at: 0 694 2329 3341 5062 6679 6781 7423 8587
Size: 694 1635 1012 1721 1617 102 642 1164
Cuts at: 8587 8991
Size: 404
Fragments arranged by size:
1721 1635 1617 1164 1012 694 642 404
102
ApaI G_GGCC'C
Cuts at: 0 439 530 1354 8991
Size: 439 91 824 7637
Fragments arranged by size:
7637 824 439 91
ApaLI G'TGCA_C
Cuts at: 0 4409 8991
Size: 4409 4582
ApoI r'AATT_y
Cuts at: 0 990 2169 2473 2552 3100 3493 3672 3791
Size: 990 1179 304 79 548 393 179 119
Cuts at: 3791 4251 4441 4584 5539 5608 5616 6798 7368
Size: 460 190 143 955 69 8 1182 570
Cuts at: 7368 8032 8991
Size: 664 959
Fragments arranged by size:
1182 1179 990 959 955 664 570 548
460 393 304 190 179 143 119 79
69 8
AvaI C'yCGr_G
Cuts at: 0 1560 5027 5831 6848 8697 8991
Size: 1560 3467 804 1017 1849 294
Fragments arranged by size:
3467 1849 1560 1017 804 294
BamHI G'GATC_C
Cuts at: 0 1827 5315 7703 8991
Size: 1827 3488 2388 1288
Fragments arranged by size:
3488 2388 1827 1288
BanI G'GyrC_C
Cuts at: 0 336 453 735 1321 1398 3989 7007 7938
Size: 336 117 282 586 77 2591 3018 931
Cuts at: 7938 7986 8991
Size: 48 1005
Fragments arranged by size:
3018 2591 1005 931 586 336 282 117
77 48
BanII G_rGCy'C
Cuts at: 0 439 474 530 1354 1682 1994 5850 7097
Size: 439 35 56 824 328 312 3856 1247
Cuts at: 7097 8797 8991
Size: 1700 194
Fragments arranged by size:
3856 1700 1247 824 439 328 312 194
56 35
BbeI G_GCGC'C
Cuts at: 0 7011 7990 8991
Size: 7011 979 1001
Fragments arranged by size:
7011 1001 979
BbsI GAAGACnn'nnnn_
Cuts at: 0 1571 2288 2747 3974 5564 5812 6752 8516
Size: 1571 717 459 1227 1590 248 940 1764
Cuts at: 8516 8558 8921 8991
Size: 42 363 70
Fragments arranged by size:
1764 1590 1571 1227 940 717 459 363
248 70 42
BbvCI CC'TCA_GC
Cuts at: 0 548 1298 7616 8825 8991
Size: 548 750 6318 1209 166
Fragments arranged by size:
6318 1209 750 548 166
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 913 947 1354 1388 7222 7256 7448 7482
Size: 913 34 407 34 5834 34 192 34
Cuts at: 7482 8991
Size: 1509
Fragments arranged by size:
5834 1509 913 407 192 34 34 34
34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1250 5943 8991
Size: 1250 4693 3048
Fragments arranged by size:
4693 3048 1250
BclI T'GATC_A
Cuts at: 0 207 4461 5000 8991
Size: 207 4254 539 3991
Fragments arranged by size:
4254 3991 539 207
BfrBI ATG'CAT
Cuts at: 0 240 1143 8991
Size: 240 903 7848
Fragments arranged by size:
7848 903 240
BglI GCCn_nnn'nGGC
Cuts at: 0 653 8318 8991
Size: 653 7665 673
Fragments arranged by size:
7665 673 653
BglII A'GATC_T
Cuts at: 0 2532 5276 8991
Size: 2532 2744 3715
Fragments arranged by size:
3715 2744 2532
BlpI GC'TnA_GC
Cuts at: 0 1733 6890 7091 8991
Size: 1733 5157 201 1900
Fragments arranged by size:
5157 1900 1733 201
Bme1580I G_kGCm'C
Cuts at: 0 341 439 530 716 1354 1421 1614 4413
Size: 341 98 91 186 638 67 193 2799
Cuts at: 4413 8991
Size: 4578
Fragments arranged by size:
4578 2799 638 341 193 186 98 91
67
BmgBI CAC'GTC
Cuts at: 0 5734 8213 8991
Size: 5734 2479 778
Fragments arranged by size:
5734 2479 778
BmrI ACTGGGnnnn_n'
Cuts at: 0 1100 1424 1826 2355 2392 3099 4303 6689
Size: 1100 324 402 529 37 707 1204 2386
Cuts at: 6689 8123 8746 8991
Size: 1434 623 245
Fragments arranged by size:
2386 1434 1204 1100 707 623 529 402
324 245 37
BmtI G_CTAG'C
Cuts at: 0 5518 8665 8991
Size: 5518 3147 326
Fragments arranged by size:
5518 3147 326
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 4045 6168 6651 8961 8991
Size: 4045 2123 483 2310 30
Fragments arranged by size:
4045 2310 2123 483 30
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2308 2471 3394 3815 3829 4165 6446 6754
Size: 2308 163 923 421 14 336 2281 308
Cuts at: 6754 8030 8266 8275 8284 8312 8714 8781 8991
Size: 1276 236 9 9 28 402 67 210
Fragments arranged by size:
2308 2281 1276 923 421 402 336 308
236 210 163 67 28 14 9 9
Bpu10I CC'TnA_GC
Cuts at: 0 548 1298 3275 4187 4529 7616 8825 8991
Size: 548 750 1977 912 342 3087 1209 166
Fragments arranged by size:
3087 1977 1209 912 750 548 342 166
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3346 3685 5280 7326 8991
Size: 3346 339 1595 2046 1665
Fragments arranged by size:
3346 2046 1665 1595 339
BsaI GGTCTCn'nnnn_
Cuts at: 0 1451 5255 7302 7642 8845 8991
Size: 1451 3804 2047 340 1203 146
Fragments arranged by size:
3804 2047 1451 1203 340 146
BsaAI yAC'GTr
Cuts at: 0 583 711 2674 2750 8991
Size: 583 128 1963 76 6241
Fragments arranged by size:
6241 1963 583 128 76
BsaBI GATnn'nnATC
Cuts at: 0 1059 4460 8991
Size: 1059 3401 4531
Fragments arranged by size:
4531 3401 1059
BsaHI Gr'CG_yC
Cuts at: 0 3564 4776 7008 7594 7987 8991
Size: 3564 1212 2232 586 393 1004
Fragments arranged by size:
3564 2232 1212 1004 586 393
BsaWI w'CCGG_w
Cuts at: 0 2654 5047 8505 8991
Size: 2654 2393 3458 486
Fragments arranged by size:
3458 2654 2393 486
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 409 439 788 818 1212 1242 1527 1557
Size: 409 30 349 30 394 30 285 30
Cuts at: 1557 2444 2474 2684 2714 4039 4069 4800 4830
Size: 887 30 210 30 1325 30 731 30
Cuts at: 4830 6103 6133 7131 7161 8991
Size: 1273 30 998 30 1830
Fragments arranged by size:
1830 1325 1273 998 887 731 409 394
349 285 210 30 30 30 30 30
30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1214 1217 2192 2195 2323 2470 2803 4438
Size: 1214 3 975 3 128 147 333 1635
Cuts at: 4438 6108 6157 6449 6541 7095 7139 7215 7218
Size: 1670 49 292 92 554 44 76 3
Cuts at: 7218 7221 7224 7545 8545 8890 8991
Size: 3 3 321 1000 345 101
Fragments arranged by size:
1670 1635 1214 1000 975 554 345 333
321 292 147 128 101 92 76 49
44 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 716 727 1252 2332 6215 6219 6540 6764
Size: 716 11 525 1080 3883 4 321 224
Cuts at: 6764 7274 8763 8991
Size: 510 1489 228
Fragments arranged by size:
3883 1489 1080 716 525 510 321 228
224 11 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 518 778 4718 5140 7625 7774 8991
Size: 518 260 3940 422 2485 149 1217
Fragments arranged by size:
3940 2485 1217 518 422 260 149
BsiEI CG_ry'CG
Cuts at: 0 4014 8192 8991
Size: 4014 4178 799
Fragments arranged by size:
4178 4014 799
BsiHKAI G_wGCw'C
Cuts at: 0 4413 5107 5850 6832 7097 8991
Size: 4413 694 743 982 265 1894
Fragments arranged by size:
4413 1894 982 743 694 265
BsmI GAATG_Cn'
Cuts at: 0 1665 1734 6086 7839 8790 8991
Size: 1665 69 4352 1753 951 201
Fragments arranged by size:
4352 1753 1665 951 201 69
BsmBI CGTCTCn'nnnn_
Cuts at: 0 302 2235 3555 3571 7151 8991
Size: 302 1933 1320 16 3580 1840
Fragments arranged by size:
3580 1933 1840 1320 302 16
Bsp1286I G_dGCh'C
Cuts at: 0 341 439 474 530 716 1354 1421 1614
Size: 341 98 35 56 186 638 67 193
Cuts at: 1614 1682 1994 4413 5107 5850 6832 7097 8797
Size: 68 312 2419 694 743 982 265 1700
Cuts at: 8797 8991
Size: 194
Fragments arranged by size:
2419 1700 982 743 694 638 341 312
265 194 193 186 98 68 67 56
35
BspHI T'CATG_A
Cuts at: 0 6900 7577 8991
Size: 6900 677 1414
Fragments arranged by size:
6900 1414 677
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1766 3510 4147 6189 6400 8991
Size: 1766 1744 637 2042 211 2591
Fragments arranged by size:
2591 2042 1766 1744 637 211
BsrBI CCG'CTC
Cuts at: 0 2757 8991
Size: 2757 6234
BsrDI GCAATG_nn'
Cuts at: 0 1208 3857 6822 8991
Size: 1208 2649 2965 2169
Fragments arranged by size:
2965 2649 2169 1208
BsrFI r'CCGG_y
Cuts at: 0 513 5112 5741 5813 8991
Size: 513 4599 629 72 3178
Fragments arranged by size:
4599 3178 629 513 72
BsrGI T'GTAC_A
Cuts at: 0 4093 8991
Size: 4093 4898
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1650 2059 2320 2329 7612 8344 8991
Size: 1650 409 261 9 5283 732 647
Fragments arranged by size:
5283 1650 732 647 409 261 9
BstEII G'GTnAC_C
Cuts at: 0 82 7620 8991
Size: 82 7538 1371
Fragments arranged by size:
7538 1371 82
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1108 1318 3865 4589 6269 7059 8016 8991
Size: 1108 210 2547 724 1680 790 957 975
Fragments arranged by size:
2547 1680 1108 975 957 790 724 210
BstYI r'GATC_y
Cuts at: 0 6 1827 2532 3192 4383 4466 4995 5276
Size: 6 1821 705 660 1191 83 529 281
Cuts at: 5276 5315 6188 6255 6876 7703 8001 8614 8991
Size: 39 873 67 621 827 298 613 377
Fragments arranged by size:
1821 1191 873 827 705 660 621 613
529 377 298 281 83 67 39 6
Bsu36I CC'TnA_GG
Cuts at: 0 332 687 1157 6932 8991
Size: 332 355 470 5775 2059
Fragments arranged by size:
5775 2059 470 355 332
BtgI C'CryG_G
Cuts at: 0 342 792 1325 3045 3292 4771 4878 5645
Size: 342 450 533 1720 247 1479 107 767
Cuts at: 5645 7100 8186 8789 8991
Size: 1455 1086 603 202
Fragments arranged by size:
1720 1479 1455 1086 767 603 533 450
342 247 202 107
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 8375 8882 8991
Size: 8375 507 109
Fragments arranged by size:
8375 507 109
BtsI GCAGTG_nn'
Cuts at: 0 357 1546 4124 4706 6830 6983 8991
Size: 357 1189 2578 582 2124 153 2008
Fragments arranged by size:
2578 2124 2008 1189 582 357 153
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 3678 3713 8991
Size: 3678 35 5278
Fragments arranged by size:
5278 3678 35
DraI TTT'AAA
Cuts at: 0 3807 8841 8991
Size: 3807 5034 150
Fragments arranged by size:
5034 3807 150
DraIII CAC_nnn'GTG
Cuts at: 0 756 3387 3924 8932 8991
Size: 756 2631 537 5008 59
Fragments arranged by size:
5008 2631 756 537 59
DrdI GACnn_nn'nnGTC
Cuts at: 0 7886 8898 8991
Size: 7886 1012 93
Fragments arranged by size:
7886 1012 93
EaeI y'GGCC_r
Cuts at: 0 696 1491 3915 4011 5019 5138 6056 6994
Size: 696 795 2424 96 1008 119 918 938
Cuts at: 6994 7107 8189 8991
Size: 113 1082 802
Fragments arranged by size:
2424 1082 1008 938 918 802 795 696
119 113 96
EagI C'GGCC_G
Cuts at: 0 4011 8189 8991
Size: 4011 4178 802
Fragments arranged by size:
4178 4011 802
EarI CTCTTCn'nnn_
Cuts at: 0 1738 2233 2257 3436 3509 3779 3794 4326
Size: 1738 495 24 1179 73 270 15 532
Cuts at: 4326 5898 5903 6566 6707 6716 6749 7072 7522
Size: 1572 5 663 141 9 33 323 450
Cuts at: 7522 8142 8710 8930 8991
Size: 620 568 220 61
Fragments arranged by size:
1738 1572 1179 663 620 568 532 495
450 323 270 220 141 73 61 33
24 15 9 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 4021 5284 8991
Size: 4021 1263 3707
Fragments arranged by size:
4021 3707 1263
EcoICRI GAG'CTC
Cuts at: 0 5848 7095 8991
Size: 5848 1247 1896
Fragments arranged by size:
5848 1896 1247
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2231 2300 2308 2471 3349 3394 3417 3455
Size: 2231 69 8 163 878 45 23 38
Cuts at: 3455 3803 3815 3829 3925 4165 4769 5212 5357
Size: 348 12 14 96 240 604 443 145
Cuts at: 5357 5609 6027 6446 6754 6866 7804 8030 8257
Size: 252 418 419 308 112 938 226 227
Cuts at: 8257 8266 8275 8284 8312 8714 8764 8781 8991
Size: 9 9 9 28 402 50 17 210
Fragments arranged by size:
2231 938 878 604 443 419 418 402
348 308 252 240 227 226 210 163
145 112 96 69 50 45 38 28
23 17 14 12 9 9 9 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 661 955 4033 4260 5164 6000 8991
Size: 661 294 3078 227 904 836 2991
Fragments arranged by size:
3078 2991 904 836 661 294 227
EcoO109I rG'GnC_Cy
Cuts at: 0 435 436 526 527 654 691 1351 1907
Size: 435 1 90 1 127 37 660 556
Cuts at: 1907 2223 3218 4400 5211 5796 8833 8991
Size: 316 995 1182 811 585 3037 158
Fragments arranged by size:
3037 1182 995 811 660 585 556 435
316 158 127 90 37 1 1
EcoRI G'AATT_C
Cuts at: 0 2473 2552 3100 8991
Size: 2473 79 548 5891
Fragments arranged by size:
5891 2473 548 79
EcoRV GAT'ATC
Cuts at: 0 937 8991
Size: 937 8054
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 6836 7101 7413 7480 8991
Size: 6836 265 312 67 1511
Fragments arranged by size:
6836 1511 312 265 67
HaeII r_GCGC'y
Cuts at: 0 2274 3300 7011 7990 8991
Size: 2274 1026 3711 979 1001
Fragments arranged by size:
3711 2274 1026 1001 979
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 409 441 2332 2364 2694 2726 3255 3287
Size: 409 32 1891 32 330 32 529 32
Cuts at: 3287 4039 4071 4420 4452 5021 5053 5718 5750
Size: 752 32 349 32 569 32 665 32
Cuts at: 5750 6001 6033 6103 6135 6324 6356 6667 6699
Size: 251 32 70 32 189 32 311 32
Cuts at: 6699 6733 6765 7239 7271 8991
Size: 34 32 474 32 1720
Fragments arranged by size:
1891 1720 752 665 569 529 474 409
349 330 311 251 189 70 34 32
32 32 32 32 32 32 32 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 969 1510 2142 2652 5040 8991
Size: 969 541 632 510 2388 3951
Fragments arranged by size:
3951 2388 969 632 541 510
HindIII A'AGCT_T
Cuts at: 0 4983 6249 7628 8991
Size: 4983 1266 1379 1363
Fragments arranged by size:
4983 1379 1363 1266
HpaI GTT'AAC
Cuts at: 0 969 8991
Size: 969 8022
KasI G'GCGC_C
Cuts at: 0 7007 7986 8991
Size: 7007 979 1005
Fragments arranged by size:
7007 1005 979
KpnI G_GTAC'C
Cuts at: 0 457 3993 8991
Size: 457 3536 4998
Fragments arranged by size:
4998 3536 457
MfeI C'AATT_G
Cuts at: 0 2943 8991
Size: 2943 6048
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1655 2375 2897 3139 3332 6310 6423 6731
Size: 1655 720 522 242 193 2978 113 308
Cuts at: 6731 8991
Size: 2260
Fragments arranged by size:
2978 2260 1655 720 522 308 242 193
113
MscI TGG'CCA
Cuts at: 0 698 1493 3917 5021 5140 6058 6996 8991
Size: 698 795 2424 1104 119 918 938 1995
Fragments arranged by size:
2424 1995 1104 938 918 795 698 119
MslI CAynn'nnrTG
Cuts at: 0 1106 3484 3733 4939 5405 7057 7264 8991
Size: 1106 2378 249 1206 466 1652 207 1727
Fragments arranged by size:
2378 1727 1652 1206 1106 466 249 207
MspA1I CmG'CkG
Cuts at: 0 5014 5144 6219 6544 6596 7197 7686 8188
Size: 5014 130 1075 325 52 601 489 502
Cuts at: 8188 8197 8304 8344 8464 8991
Size: 9 107 40 120 527
Fragments arranged by size:
5014 1075 601 527 502 489 325 130
120 107 52 40 9
NaeI GCC'GGC
Cuts at: 0 515 5743 8991
Size: 515 5228 3248
Fragments arranged by size:
5228 3248 515
NarI GG'CG_CC
Cuts at: 0 7008 7987 8991
Size: 7008 979 1004
Fragments arranged by size:
7008 1004 979
NcoI C'CATG_G
Cuts at: 0 342 792 1325 3045 3292 5645 7100 8991
Size: 342 450 533 1720 247 2353 1455 1891
Fragments arranged by size:
2353 1891 1720 1455 533 450 342 247
NdeI CA'TA_TG
Cuts at: 0 3996 8991
Size: 3996 4995
NgoMIV G'CCGG_C
Cuts at: 0 513 5741 8991
Size: 513 5228 3250
Fragments arranged by size:
5228 3250 513
NheI G'CTAG_C
Cuts at: 0 5514 8661 8991
Size: 5514 3147 330
Fragments arranged by size:
5514 3147 330
NotI GC'GGCC_GC
Cuts at: 0 8189 8991
Size: 8189 802
NsiI A_TGCA'T
Cuts at: 0 242 1145 8991
Size: 242 903 7846
Fragments arranged by size:
7846 903 242
NspI r_CATG'y
Cuts at: 0 387 558 1365 1392 2958 5232 5514 5659
Size: 387 171 807 27 1566 2274 282 145
Cuts at: 5659 8991
Size: 3332
Fragments arranged by size:
3332 2274 1566 807 387 282 171 145
27
PasI CC'CwG_GG
Cuts at: 0 280 440 3198 8991
Size: 280 160 2758 5793
Fragments arranged by size:
5793 2758 280 160
PfoI T'CCnGG_A
Cuts at: 0 9 1472 5165 7677 8328 8730 8991
Size: 9 1463 3693 2512 651 402 261
Fragments arranged by size:
3693 2512 1463 651 402 261 9
PmlI CAC'GTG
Cuts at: 0 583 711 2750 8991
Size: 583 128 2039 6241
Fragments arranged by size:
6241 2039 583 128
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2409 2441 8551 8583 8991
Size: 2409 32 6110 32 408
Fragments arranged by size:
6110 2409 408 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 691 1907 2223 3218 5211 8833 8991
Size: 691 1216 316 995 1993 3622 158
Fragments arranged by size:
3622 1993 1216 995 691 316 158
PshAI GACnn'nnGTC
Cuts at: 0 294 8991
Size: 294 8697
PspOMI G'GGCC_C
Cuts at: 0 435 526 1350 8991
Size: 435 91 824 7641
Fragments arranged by size:
7641 824 435 91
PspXI vC'TCGA_Gb
Cuts at: 0 5027 8991
Size: 5027 3964
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1469 1501 8991
Size: 1469 32 7490
Fragments arranged by size:
7490 1469 32
PstI C_TGCA'G
Cuts at: 0 1553 4930 5349 8898 8991
Size: 1553 3377 419 3549 93
Fragments arranged by size:
3549 3377 1553 419 93
PvuII CAG'CTG
Cuts at: 0 5144 6219 6544 7686 8304 8344 8464 8991
Size: 5144 1075 325 1142 618 40 120 527
Fragments arranged by size:
5144 1142 1075 618 527 325 120 40
SacI G_AGCT'C
Cuts at: 0 5850 7097 8991
Size: 5850 1247 1894
Fragments arranged by size:
5850 1894 1247
SacII CC_GC'GG
Cuts at: 0 8189 8991
Size: 8189 802
SanDI GG'GwC_CC
Cuts at: 0 1907 8991
Size: 1907 7084
SapI GCTCTTCn'nnn_
Cuts at: 0 3509 8930 8991
Size: 3509 5421 61
Fragments arranged by size:
5421 3509 61
SexAI A'CCwGG_T
Cuts at: 0 4649 8991
Size: 4649 4342
SfcI C'TryA_G
Cuts at: 0 1049 1549 2525 4233 4315 4692 4926 5345
Size: 1049 500 976 1708 82 377 234 419
Cuts at: 5345 8120 8894 8991
Size: 2775 774 97
Fragments arranged by size:
2775 1708 1049 976 774 500 419 377
234 97 82
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 653 8991
Size: 653 8338
SfoI GGC'GCC
Cuts at: 0 7009 7988 8991
Size: 7009 979 1003
Fragments arranged by size:
7009 1003 979
SmaI CCC'GGG
Cuts at: 0 1562 8699 8991
Size: 1562 7137 292
Fragments arranged by size:
7137 1562 292
SmlI C'TyrA_G
Cuts at: 0 3361 3664 5027 5295 5831 6232 7341 8991
Size: 3361 303 1363 268 536 401 1109 1650
Fragments arranged by size:
3361 1650 1363 1109 536 401 303 268
SphI G_CATG'C
Cuts at: 0 387 5232 8991
Size: 387 4845 3759
Fragments arranged by size:
4845 3759 387
SspI AAT'ATT
Cuts at: 0 45 3288 3649 8991
Size: 45 3243 361 5342
Fragments arranged by size:
5342 3243 361 45
StuI AGG'CCT
Cuts at: 0 68 998 7031 7339 7463 8312 8991
Size: 68 930 6033 308 124 849 679
Fragments arranged by size:
6033 930 849 679 308 124 68
StyI C'CwwG_G
Cuts at: 0 132 342 642 792 1325 1402 1452 1606
Size: 132 210 300 150 533 77 50 154
Cuts at: 1606 1976 1994 3045 3292 4571 5033 5068 5477
Size: 370 18 1051 247 1279 462 35 409
Cuts at: 5477 5645 6548 7026 7100 8991
Size: 168 903 478 74 1891
Fragments arranged by size:
1891 1279 1051 903 533 478 462 409
370 300 247 210 168 154 150 132
77 74 50 35 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 358 1206 1637 1740 5022 6305 7065 7491
Size: 358 848 431 103 3282 1283 760 426
Cuts at: 7491 7963 8991
Size: 472 1028
Fragments arranged by size:
3282 1283 1028 848 760 472 431 426
358 103
TatI w'GTAC_w
Cuts at: 0 3182 4093 4943 5234 5414 8991
Size: 3182 911 850 291 180 3577
Fragments arranged by size:
3577 3182 911 850 291 180
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1212 1244 1454 1486 2684 2716 5075 5107
Size: 1212 32 210 32 1198 32 2359 32
Cuts at: 5107 7131 7163 8991
Size: 2024 32 1828
Fragments arranged by size:
2359 2024 1828 1212 1198 210 32 32
32 32 32
XbaI T'CTAG_A
Cuts at: 0 1686 6787 8991
Size: 1686 5101 2204
Fragments arranged by size:
5101 2204 1686
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 349 1501 8991
Size: 349 1152 7490
Fragments arranged by size:
7490 1152 349
XhoI C'TCGA_G
Cuts at: 0 5027 5831 8991
Size: 5027 804 3160
Fragments arranged by size:
5027 3160 804
XmaI C'CCGG_G
Cuts at: 0 1560 8697 8991
Size: 1560 7137 294
Fragments arranged by size:
7137 1560 294
XmnI GAAnn'nnTTC
Cuts at: 0 46 2599 3673 6040 8991
Size: 46 2553 1074 2367 2951
Fragments arranged by size:
2951 2553 2367 1074 46
ZraI GAC'GTC
Cuts at: 0 3565 7595 8991
Size: 3565 4030 1396
Fragments arranged by size:
4030 3565 1396
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AflII AflIII
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PfoI PmlI
PpiI PpuMI PshAI PspOMI PspXI PsrI PstI PvuII
SacI SacII SanDI SapI SexAI SfcI SfiI SfoI
SmaI SmlI SphI SspI StuI StyI TaqII TatI
TstI XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AclI AgeI AloI AscI AseI AsiSI AvrII BaeI
BsiWI BspEI BssHII BssSI BstBI BstZ17I ClaI FseI
FspI FspAI MluI NruI PacI PciI PflMI PmeI
PsiI PvuI RsrII SalI SbfI ScaI SgfI SgrAI
SnaBI SpeI SrfI SwaI Tth111I