Restriction map for
pF1KE9678
(Linear) (Six-base) MAPSORT of: KIEE9678.seq Check: 5886 from: 1 to: 4251
KIEE9678.orf
With 182 enzymes: SgfI *
April 12, 2016 09:51 ..
AccI GT'mk_AC
Cuts at: 0 3344 3560 4251
Size: 3344 216 691
Fragments arranged by size:
3344 691 216
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 110 201 752 1186 2554 3821 3896 4251
Size: 110 91 551 434 1368 1267 75 355
Fragments arranged by size:
1368 1267 551 434 355 110 91 75
AflII C'TTAA_G
Cuts at: 0 4228 4251
Size: 4228 23
AflIII A'CryG_T
Cuts at: 0 2717 3303 4087 4251
Size: 2717 586 784 164
Fragments arranged by size:
2717 784 586 164
AhdI GACnn_n'nnGTC
Cuts at: 0 1817 4251
Size: 1817 2434
AleI CACnn'nnGTG
Cuts at: 0 1585 4251
Size: 1585 2666
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2646 4251
Size: 2646 1605
AlwNI CAG_nnn'CTG
Cuts at: 0 1056 4251
Size: 1056 3195
ApoI r'AATT_y
Cuts at: 0 91 418 819 868 1341 1359 1380 1896
Size: 91 327 401 49 473 18 21 516
Cuts at: 1896 1984 3579 4251
Size: 88 1595 672
Fragments arranged by size:
1595 672 516 473 401 327 91 88
49 21 18
AseI AT'TA_AT
Cuts at: 0 1144 2804 4251
Size: 1144 1660 1447
Fragments arranged by size:
1660 1447 1144
AvaI C'yCGr_G
Cuts at: 0 392 4251
Size: 392 3859
AvrII C'CTAG_G
Cuts at: 0 1419 4251
Size: 1419 2832
BamHI G'GATC_C
Cuts at: 0 4132 4251
Size: 4132 119
BanII G_rGCy'C
Cuts at: 0 2738 3944 4251
Size: 2738 1206 307
Fragments arranged by size:
2738 1206 307
BbsI GAAGACnn'nnnn_
Cuts at: 0 212 1258 1709 1813 2054 3476 3809 3884
Size: 212 1046 451 104 241 1422 333 75
Cuts at: 3884 4251
Size: 367
Fragments arranged by size:
1422 1046 451 367 333 241 212 104
75
BbvCI CC'TCA_GC
Cuts at: 0 1803 3935 4251
Size: 1803 2132 316
Fragments arranged by size:
2132 1803 316
BciVI GTATCCnnnnn_n'
Cuts at: 0 390 4251
Size: 390 3861
BfrBI ATG'CAT
Cuts at: 0 1713 2890 4243 4251
Size: 1713 1177 1353 8
Fragments arranged by size:
1713 1353 1177 8
BglII A'GATC_T
Cuts at: 0 1017 2306 4251
Size: 1017 1289 1945
Fragments arranged by size:
1945 1289 1017
BmgBI CAC'GTC
Cuts at: 0 2871 4251
Size: 2871 1380
BmrI ACTGGGnnnn_n'
Cuts at: 0 1521 1707 4251
Size: 1521 186 2544
Fragments arranged by size:
2544 1521 186
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2752 4251
Size: 2752 1499
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 263 896 2093 2236 2282 2359 2673 4104
Size: 263 633 1197 143 46 77 314 1431
Cuts at: 4104 4251
Size: 147
Fragments arranged by size:
1431 1197 633 314 263 147 143 77
46
Bpu10I CC'TnA_GC
Cuts at: 0 755 1803 3935 4251
Size: 755 1048 2132 316
Fragments arranged by size:
2132 1048 755 316
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 391 2166 3721 4142 4251
Size: 391 1775 1555 421 109
Fragments arranged by size:
1775 1555 421 391 109
BsaBI GATnn'nnATC
Cuts at: 0 312 709 4251
Size: 312 397 3542
Fragments arranged by size:
3542 397 312
BsaWI w'CCGG_w
Cuts at: 0 2472 2865 4251
Size: 2472 393 1386
Fragments arranged by size:
2472 1386 393
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2671 2701 3351 3381 4251
Size: 2671 30 650 30 870
Fragments arranged by size:
2671 870 650 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 624 3952 4053 4251
Size: 624 3328 101 198
Fragments arranged by size:
3328 624 198 101
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2018 2809 4251
Size: 2018 791 1442
Fragments arranged by size:
2018 1442 791
BsiHKAI G_wGCw'C
Cuts at: 0 1133 3944 4251
Size: 1133 2811 307
Fragments arranged by size:
2811 1133 307
BsmI GAATG_Cn'
Cuts at: 0 3109 4243 4251
Size: 3109 1134 8
Fragments arranged by size:
3109 1134 8
Bsp1286I G_dGCh'C
Cuts at: 0 1133 2738 3944 4251
Size: 1133 1605 1206 307
Fragments arranged by size:
1605 1206 1133 307
BspHI T'CATG_A
Cuts at: 0 2985 4251
Size: 2985 1266
BsrDI GCAATG_nn'
Cuts at: 0 725 4251
Size: 725 3526
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2929 4251
Size: 2929 1322
BstYI r'GATC_y
Cuts at: 0 1017 2306 3400 4132 4251
Size: 1017 1289 1094 732 119
Fragments arranged by size:
1289 1094 1017 732 119
Bsu36I CC'TnA_GG
Cuts at: 0 2549 3770 4251
Size: 2549 1221 481
Fragments arranged by size:
2549 1221 481
BtgI C'CryG_G
Cuts at: 0 2708 4251
Size: 2708 1543
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 713 3851 4251
Size: 713 3138 400
Fragments arranged by size:
3138 713 400
BtsI GCAGTG_nn'
Cuts at: 0 542 1580 2035 2961 3594 4251
Size: 542 1038 455 926 633 657
Fragments arranged by size:
1038 926 657 633 542 455
ClaI AT'CG_AT
Cuts at: 0 713 4251
Size: 713 3538
DraI TTT'AAA
Cuts at: 0 111 270 582 3438 3554 4251
Size: 111 159 312 2856 116 697
Fragments arranged by size:
2856 697 312 159 116 111
DraIII CAC_nnn'GTG
Cuts at: 0 2395 4251
Size: 2395 1856
EaeI y'GGCC_r
Cuts at: 0 1771 2489 4251
Size: 1771 718 1762
Fragments arranged by size:
1771 1762 718
EarI CTCTTCn'nnn_
Cuts at: 0 948 2937 3629 3997 4251
Size: 948 1989 692 368 254
Fragments arranged by size:
1989 948 692 368 254
EcoICRI GAG'CTC
Cuts at: 0 3942 4251
Size: 3942 309
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 110 201 263 752 896 1186 2093 2236
Size: 110 91 62 489 144 290 907 143
Cuts at: 2236 2282 2359 2554 2673 3821 3896 4104 4251
Size: 46 77 195 119 1148 75 208 147
Fragments arranged by size:
1148 907 489 290 208 195 147 144
143 119 110 91 77 75 62 46
EcoNI CCTnn'n_nnAGG
Cuts at: 0 4026 4251
Size: 4026 225
EcoO109I rG'GnC_Cy
Cuts at: 0 969 3310 3916 4251
Size: 969 2341 606 335
Fragments arranged by size:
2341 969 606 335
EcoRI G'AATT_C
Cuts at: 0 1341 4251
Size: 1341 2910
EcoRV GAT'ATC
Cuts at: 0 4100 4251
Size: 4100 151
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 282 591 607 3579 4251
Size: 282 309 16 2972 672
Fragments arranged by size:
2972 672 309 282 16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1009 1041 1803 1835 2141 2173 4182 4214
Size: 1009 32 762 32 306 32 2009 32
Cuts at: 4214 4251
Size: 37
Fragments arranged by size:
2009 1009 762 306 37 32 32 32
32
HincII GTy'rAC
Cuts at: 0 3345 4251
Size: 3345 906
HindIII A'AGCT_T
Cuts at: 0 3742 4251
Size: 3742 509
MfeI C'AATT_G
Cuts at: 0 2837 4251
Size: 2837 1414
MslI CAynn'nnrTG
Cuts at: 0 743 1585 2660 3086 3176 4251
Size: 743 842 1075 426 90 1075
Fragments arranged by size:
1075 1075 842 743 426 90
MspA1I CmG'CkG
Cuts at: 0 1209 1744 2532 3874 3934 4251
Size: 1209 535 788 1342 60 317
Fragments arranged by size:
1342 1209 788 535 317 60
NcoI C'CATG_G
Cuts at: 0 2708 4251
Size: 2708 1543
NdeI CA'TA_TG
Cuts at: 0 2661 4239 4251
Size: 2661 1578 12
Fragments arranged by size:
2661 1578 12
NsiI A_TGCA'T
Cuts at: 0 1715 2892 4245 4251
Size: 1715 1177 1353 6
Fragments arranged by size:
1715 1353 1177 6
NspI r_CATG'y
Cuts at: 0 3307 4091 4251
Size: 3307 784 160
Fragments arranged by size:
3307 784 160
PasI CC'CwG_GG
Cuts at: 0 2504 3889 4251
Size: 2504 1385 362
Fragments arranged by size:
2504 1385 362
PciI A'CATG_T
Cuts at: 0 3303 4087 4251
Size: 3303 784 164
Fragments arranged by size:
3303 784 164
PpuMI rG'GwC_Cy
Cuts at: 0 969 3310 4251
Size: 969 2341 941
Fragments arranged by size:
2341 969 941
PsiI TTA'TAA
Cuts at: 0 3013 3215 4251
Size: 3013 202 1036
Fragments arranged by size:
3013 1036 202
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 3245 3277 4251
Size: 3245 32 974
Fragments arranged by size:
3245 974 32
PvuII CAG'CTG
Cuts at: 0 1744 2532 3874 4251
Size: 1744 788 1342 377
Fragments arranged by size:
1744 1342 788 377
SacI G_AGCT'C
Cuts at: 0 3944 4251
Size: 3944 307
SalI G'TCGA_C
Cuts at: 0 3343 4251
Size: 3343 908
SfcI C'TryA_G
Cuts at: 0 25 1334 4251
Size: 25 1309 2917
Fragments arranged by size:
2917 1309 25
SmaI CCC'GGG
Cuts at: 0 394 4251
Size: 394 3857
SmlI C'TyrA_G
Cuts at: 0 406 2145 3736 4157 4228 4251
Size: 406 1739 1591 421 71 23
Fragments arranged by size:
1739 1591 421 406 71 23
SspI AAT'ATT
Cuts at: 0 2244 3717 4251
Size: 2244 1473 534
Fragments arranged by size:
2244 1473 534
StuI AGG'CCT
Cuts at: 0 1293 1467 4251
Size: 1293 174 2784
Fragments arranged by size:
2784 1293 174
StyI C'CwwG_G
Cuts at: 0 1419 2186 2708 4064 4251
Size: 1419 767 522 1356 187
Fragments arranged by size:
1419 1356 767 522 187
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2672 3188 4251
Size: 2672 516 1063
Fragments arranged by size:
2672 1063 516
TatI w'GTAC_w
Cuts at: 0 3537 4251
Size: 3537 714
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2484 2516 3351 3383 4251
Size: 2484 32 835 32 868
Fragments arranged by size:
2484 868 835 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 4182 4251
Size: 4182 69
XmaI C'CCGG_G
Cuts at: 0 392 4251
Size: 392 3859
XmnI GAAnn'nnTTC
Cuts at: 0 1199 4251
Size: 1199 3052
Enzymes that do cut:
AccI AcuI AflII AflIII AhdI AleI AlfI AlwNI
ApoI AseI AvaI AvrII BamHI BanII BbsI BbvCI
BciVI BfrBI BglII BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaBI BsaWI BsaXI BseRI BsgI BsiHKAI BsmI
Bsp1286I BspHI BsrDI BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI DraI DraIII EaeI EarI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI Hin4I HincII HindIII
MfeI MslI MspA1I NcoI NdeI NsiI NspI PasI
PciI PpuMI PsiI PsrI PvuII SacI SalI SfcI
SmaI SmlI SspI StuI StyI TaqII TatI TstI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AloI ApaI
ApaLI AscI AsiSI BaeI BanI BbeI BcgI BclI
BglI BlpI Bme1580I BmtI BsaI BsaAI BsaHI BseYI
BsiEI BsiWI BsmBI BspEI BspMI BsrBI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstZ17I CspCI DrdI
EagI EciI FseI FspI FspAI HaeII HpaI KasI
KpnI MluI MmeI MscI NaeI NarI NgoMIV NheI
NotI NruI PacI PflMI PfoI PmeI PmlI PpiI
PshAI PspOMI PspXI PstI PvuI RsrII SacII SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI SrfI SwaI Tth111I XbaI XhoI
ZraI