Restriction map for
pF1KE9696
(Linear) (Six-base) MAPSORT of: KIEE9696.seq Check: 4381 from: 1 to: 5988
KIEE9696.orf
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 657 1183 4437 5988
Size: 657 526 3254 1551
Fragments arranged by size:
3254 1551 657 526
AccI GT'mk_AC
Cuts at: 0 4131 4921 5988
Size: 4131 790 1067
Fragments arranged by size:
4131 1067 790
Acc65I G'GTAC_C
Cuts at: 0 4886 5988
Size: 4886 1102
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 477 1522 1675 3223 3707 4142 5342 5659
Size: 477 1045 153 1548 484 435 1200 317
Cuts at: 5659 5775 5988
Size: 116 213
Fragments arranged by size:
1548 1200 1045 484 477 435 317 213
153 116
AfeI AGC'GCT
Cuts at: 0 4567 5988
Size: 4567 1421
AflII C'TTAA_G
Cuts at: 0 3061 5988
Size: 3061 2927
AflIII A'CryG_T
Cuts at: 0 1403 2198 5988
Size: 1403 795 3790
Fragments arranged by size:
3790 1403 795
AgeI A'CCGG_T
Cuts at: 0 3533 5988
Size: 3533 2455
AhdI GACnn_n'nnGTC
Cuts at: 0 4065 5988
Size: 4065 1923
AleI CACnn'nnGTG
Cuts at: 0 3574 3811 5988
Size: 3574 237 2177
Fragments arranged by size:
3574 2177 237
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 433 465 2380 2412 2647 2679 5988
Size: 433 32 1915 32 235 32 3309
Fragments arranged by size:
3309 1915 433 235 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 16 619 622 655 679 949 2182 4305
Size: 16 603 3 33 24 270 1233 2123
Cuts at: 4305 4637 4777 5397 5988
Size: 332 140 620 591
Fragments arranged by size:
2123 1233 620 603 591 332 270 140
33 24 16 3
ApaI G_GGCC'C
Cuts at: 0 2244 3369 5814 5988
Size: 2244 1125 2445 174
Fragments arranged by size:
2445 2244 1125 174
ApoI r'AATT_y
Cuts at: 0 226 2416 2476 2994 3009 3676 5988
Size: 226 2190 60 518 15 667 2312
Fragments arranged by size:
2312 2190 667 518 226 60 15
AvaI C'yCGr_G
Cuts at: 0 824 921 3395 4190 4424 5051 5184 5957
Size: 824 97 2474 795 234 627 133 773
Cuts at: 5957 5988
Size: 31
Fragments arranged by size:
2474 824 795 773 627 234 133 97
31
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 4870 4903 5988
Size: 4870 33 1085
Fragments arranged by size:
4870 1085 33
BanI G'GyrC_C
Cuts at: 0 867 2188 2409 3322 4848 4857 4886 5827
Size: 867 1321 221 913 1526 9 29 941
Cuts at: 5827 5988
Size: 161
Fragments arranged by size:
1526 1321 941 913 867 221 161 29
9
BanII G_rGCy'C
Cuts at: 0 823 1227 1391 1757 2244 2753 2933 3369
Size: 823 404 164 366 487 509 180 436
Cuts at: 3369 3498 3631 5814 5957 5988
Size: 129 133 2183 143 31
Fragments arranged by size:
2183 823 509 487 436 404 366 180
164 143 133 129 31
BbeI G_GCGC'C
Cuts at: 0 5831 5988
Size: 5831 157
BbsI GAAGACnn'nnnn_
Cuts at: 0 346 2063 2298 3881 3986 4058 4109 5220
Size: 346 1717 235 1583 105 72 51 1111
Cuts at: 5220 5988
Size: 768
Fragments arranged by size:
1717 1583 1111 768 346 235 105 72
51
BbvCI CC'TCA_GC
Cuts at: 0 52 950 3952 5698 5988
Size: 52 898 3002 1746 290
Fragments arranged by size:
3002 1746 898 290 52
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1472 1506 5988
Size: 1472 34 4482
Fragments arranged by size:
4482 1472 34
BclI T'GATC_A
Cuts at: 0 1347 2579 2589 5988
Size: 1347 1232 10 3399
Fragments arranged by size:
3399 1347 1232 10
BglII A'GATC_T
Cuts at: 0 5201 5988
Size: 5201 787
BlpI GC'TnA_GC
Cuts at: 0 662 2950 5602 5988
Size: 662 2288 2652 386
Fragments arranged by size:
2652 2288 662 386
Bme1580I G_kGCm'C
Cuts at: 0 2244 2251 2364 2412 3150 3327 3369 5722
Size: 2244 7 113 48 738 177 42 2353
Cuts at: 5722 5814 5988
Size: 92 174
Fragments arranged by size:
2353 2244 738 177 174 113 92 48
42 7
BmgBI CAC'GTC
Cuts at: 0 3813 5622 5988
Size: 3813 1809 366
Fragments arranged by size:
3813 1809 366
BmrI ACTGGGnnnn_n'
Cuts at: 0 2114 2394 3119 3948 5988
Size: 2114 280 725 829 2040
Fragments arranged by size:
2114 2040 829 725 280
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1265 5988
Size: 1265 4723
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 53 451 1211 2049 2204 2764 2830 3166
Size: 53 398 760 838 155 560 66 336
Cuts at: 3166 4557 4643 4837 4883 5365 5407 5611 5870
Size: 1391 86 194 46 482 42 204 259
Cuts at: 5870 5988
Size: 118
Fragments arranged by size:
1391 838 760 560 482 398 336 259
204 194 155 118 86 66 53 46
42
Bpu10I CC'TnA_GC
Cuts at: 0 52 950 1211 3952 4046 5698 5988
Size: 52 898 261 2741 94 1652 290
Fragments arranged by size:
2741 1652 898 290 261 94 52
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2635 2875 3283 4720 5988
Size: 2635 240 408 1437 1268
Fragments arranged by size:
2635 1437 1268 408 240
BsaAI yAC'GTr
Cuts at: 0 1973 5988
Size: 1973 4015
BsaBI GATnn'nnATC
Cuts at: 0 559 1583 5988
Size: 559 1024 4405
Fragments arranged by size:
4405 1024 559
BsaHI Gr'CG_yC
Cuts at: 0 4924 5828 5988
Size: 4924 904 160
Fragments arranged by size:
4924 904 160
BsaWI w'CCGG_w
Cuts at: 0 289 709 3533 3782 4151 5988
Size: 289 420 2824 249 369 1837
Fragments arranged by size:
2824 1837 420 369 289 249
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 433 463 1378 1408 1759 1789 1831 1861
Size: 433 30 915 30 351 30 42 30
Cuts at: 1861 2380 2410 4647 4677 4936 4966 5988
Size: 519 30 2237 30 259 30 1022
Fragments arranged by size:
2237 1022 915 519 433 351 259 42
30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 235 472 1105 1108 1324 1351 1354 1404
Size: 235 237 633 3 216 27 3 50
Cuts at: 1404 1855 2761 2827 3742 3931 4105 4108 4560
Size: 451 906 66 915 189 174 3 452
Cuts at: 4560 4572 4957 5422 5608 5988
Size: 12 385 465 186 380
Fragments arranged by size:
915 906 633 465 452 451 385 380
237 235 216 189 186 174 66 50
27 12 3 3 3
BseYI C'CCAG_C
Cuts at: 0 2066 2615 2865 3400 3624 4215 4265 4549
Size: 2066 549 250 535 224 591 50 284
Cuts at: 4549 4786 4791 5236 5988
Size: 237 5 445 752
Fragments arranged by size:
2066 752 591 549 535 445 284 250
237 224 50 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1534 2179 3835 4513 4623 5554 5988
Size: 1534 645 1656 678 110 931 434
Fragments arranged by size:
1656 1534 931 678 645 434 110
BsiEI CG_ry'CG
Cuts at: 0 3348 5988
Size: 3348 2640
BsiHKAI G_wGCw'C
Cuts at: 0 1391 2753 2933 3227 4508 5988
Size: 1391 1362 180 294 1281 1480
Fragments arranged by size:
1480 1391 1362 1281 294 180
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1105 4604 5988
Size: 1105 3499 1384
Fragments arranged by size:
3499 1384 1105
Bsp1286I G_dGCh'C
Cuts at: 0 823 1227 1391 1757 2244 2251 2364 2412
Size: 823 404 164 366 487 7 113 48
Cuts at: 2412 2753 2933 3150 3227 3327 3369 3498 3631
Size: 341 180 217 77 100 42 129 133
Cuts at: 3631 4508 5722 5814 5957 5988
Size: 877 1214 92 143 31
Fragments arranged by size:
1214 877 823 487 404 366 341 217
180 164 143 133 129 113 100 92
77 48 42 31 7
BspEI T'CCGG_A
Cuts at: 0 709 4151 5988
Size: 709 3442 1837
Fragments arranged by size:
3442 1837 709
BspHI T'CATG_A
Cuts at: 0 503 3050 5988
Size: 503 2547 2938
Fragments arranged by size:
2938 2547 503
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 657 1183 4437 4659 5988
Size: 657 526 3254 222 1329
Fragments arranged by size:
3254 1329 657 526 222
BsrBI CCG'CTC
Cuts at: 0 2391 4812 5988
Size: 2391 2421 1176
Fragments arranged by size:
2421 2391 1176
BsrFI r'CCGG_y
Cuts at: 0 995 1037 3533 5451 5553 5702 5739 5988
Size: 995 42 2496 1918 102 149 37 249
Fragments arranged by size:
2496 1918 995 249 149 102 42 37
BssSI C'ACGA_G
Cuts at: 0 1181 3014 5988
Size: 1181 1833 2974
Fragments arranged by size:
2974 1833 1181
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2862 5988
Size: 2862 3126
BstEII G'GTnAC_C
Cuts at: 0 5026 5988
Size: 5026 962
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1150 5988
Size: 1150 4838
BstYI r'GATC_y
Cuts at: 0 536 1520 3057 5201 5988
Size: 536 984 1537 2144 787
Fragments arranged by size:
2144 1537 984 787 536
Bsu36I CC'TnA_GG
Cuts at: 0 990 3328 4318 4613 5988
Size: 990 2338 990 295 1375
Fragments arranged by size:
2338 1375 990 990 295
BtgI C'CryG_G
Cuts at: 0 2164 3095 4890 5062 5250 5465 5988
Size: 2164 931 1795 172 188 215 523
Fragments arranged by size:
2164 1795 931 523 215 188 172
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3750 5646 5988
Size: 3750 1896 342
Fragments arranged by size:
3750 1896 342
BtsI GCAGTG_nn'
Cuts at: 0 282 981 1085 1562 1668 3425 3744 4171
Size: 282 699 104 477 106 1757 319 427
Cuts at: 4171 4180 5988
Size: 9 1808
Fragments arranged by size:
1808 1757 699 477 427 319 282 106
104 9
ClaI AT'CG_AT
Cuts at: 0 3305 3344 5988
Size: 3305 39 2644
Fragments arranged by size:
3305 2644 39
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1411 1446 5988
Size: 1411 35 4542
Fragments arranged by size:
4542 1411 35
DraI TTT'AAA
Cuts at: 0 5316 5988
Size: 5316 672
DraIII CAC_nnn'GTG
Cuts at: 0 1908 3019 3222 4301 5153 5988
Size: 1908 1111 203 1079 852 835
Fragments arranged by size:
1908 1111 1079 852 835 203
DrdI GACnn_nn'nnGTC
Cuts at: 0 3549 5988
Size: 3549 2439
EaeI y'GGCC_r
Cuts at: 0 689 1039 1082 3422 3507 3578 5504 5555
Size: 689 350 43 2340 85 71 1926 51
Cuts at: 5555 5708 5816 5988
Size: 153 108 172
Fragments arranged by size:
2340 1926 689 350 172 153 108 85
71 51 43
EarI CTCTTCn'nnn_
Cuts at: 0 139 762 1301 1616 1789 2524 2758 3554
Size: 139 623 539 315 173 735 234 796
Cuts at: 3554 3683 5988
Size: 129 2305
Fragments arranged by size:
2305 796 735 623 539 315 234 173
139 129
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 366 825 5632 5988
Size: 366 459 4807 356
Fragments arranged by size:
4807 459 366 356
EcoICRI GAG'CTC
Cuts at: 0 1389 2751 2931 5988
Size: 1389 1362 180 3057
Fragments arranged by size:
3057 1389 1362 180
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 53 451 477 1211 1522 1675 2049 2204
Size: 53 398 26 734 311 153 374 155
Cuts at: 2204 2764 2830 3166 3223 3707 4142 4557 4643
Size: 560 66 336 57 484 435 415 86
Cuts at: 4643 4837 4883 5342 5365 5407 5611 5659 5775
Size: 194 46 459 23 42 204 48 116
Cuts at: 5775 5870 5988
Size: 95 118
Fragments arranged by size:
734 560 484 459 435 415 398 374
336 311 204 194 155 153 118 116
95 86 66 57 53 48 46 42
26 23
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1613 3331 5389 5443 5527 5572 5988
Size: 1613 1718 2058 54 84 45 416
Fragments arranged by size:
2058 1718 1613 416 84 54 45
EcoO109I rG'GnC_Cy
Cuts at: 0 157 426 509 934 1062 2344 3365 3405
Size: 157 269 83 425 128 1282 1021 40
Cuts at: 3405 4314 4419 5047 5755 5810 5811 5855 5988
Size: 909 105 628 708 55 1 44 133
Fragments arranged by size:
1282 1021 909 708 628 425 269 157
133 128 105 83 55 44 40 1
EcoRI G'AATT_C
Cuts at: 0 2476 3009 5988
Size: 2476 533 2979
Fragments arranged by size:
2979 2476 533
EcoRV GAT'ATC
Cuts at: 0 747 5988
Size: 747 5241
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 5355 5988
Size: 5355 633
FseI GG_CCGG'CC
Cuts at: 0 999 1041 5988
Size: 999 42 4947
Fragments arranged by size:
4947 999 42
HaeII r_GCGC'y
Cuts at: 0 4556 4569 5398 5798 5831 5988
Size: 4556 13 829 400 33 157
Fragments arranged by size:
4556 829 400 157 33 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 655 687 1759 1791 3212 3244 3890 3922
Size: 655 32 1072 32 1421 32 646 32
Cuts at: 3922 4051 4083 5050 5082 5988
Size: 129 32 967 32 906
Fragments arranged by size:
1421 1072 967 906 655 646 129 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 4029 4454 4922 5988
Size: 4029 425 468 1066
Fragments arranged by size:
4029 1066 468 425
HindIII A'AGCT_T
Cuts at: 0 847 2516 5988
Size: 847 1669 3472
Fragments arranged by size:
3472 1669 847
HpaI GTT'AAC
Cuts at: 0 4029 5988
Size: 4029 1959
KasI G'GCGC_C
Cuts at: 0 5827 5988
Size: 5827 161
KpnI G_GTAC'C
Cuts at: 0 4890 5988
Size: 4890 1098
MluI A'CGCG_T
Cuts at: 0 1403 5988
Size: 1403 4585
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 2189 3758 4934 5988
Size: 280 1909 1569 1176 1054
Fragments arranged by size:
1909 1569 1176 1054 280
MscI TGG'CCA
Cuts at: 0 3424 3509 3580 5710 5988
Size: 3424 85 71 2130 278
Fragments arranged by size:
3424 2130 278 85 71
MslI CAynn'nnrTG
Cuts at: 0 3574 3811 3962 4210 4376 4511 5255 5988
Size: 3574 237 151 248 166 135 744 733
Fragments arranged by size:
3574 744 733 248 237 166 151 135
MspA1I CmG'CkG
Cuts at: 0 51 619 637 655 676 1695 1734 2615
Size: 51 568 18 18 21 1019 39 881
Cuts at: 2615 4236 5415 5589 5988
Size: 1621 1179 174 399
Fragments arranged by size:
1621 1179 1019 881 568 399 174 51
39 21 18 18
NaeI GCC'GGC
Cuts at: 0 997 1039 5453 5555 5704 5988
Size: 997 42 4414 102 149 284
Fragments arranged by size:
4414 997 284 149 102 42
NarI GG'CG_CC
Cuts at: 0 5828 5988
Size: 5828 160
NcoI C'CATG_G
Cuts at: 0 5250 5465 5988
Size: 5250 215 523
Fragments arranged by size:
5250 523 215
NdeI CA'TA_TG
Cuts at: 0 1959 5988
Size: 1959 4029
NgoMIV G'CCGG_C
Cuts at: 0 995 1037 5451 5553 5702 5988
Size: 995 42 4414 102 149 286
Fragments arranged by size:
4414 995 286 149 102 42
NspI r_CATG'y
Cuts at: 0 448 1493 2202 2880 2916 5988
Size: 448 1045 709 678 36 3072
Fragments arranged by size:
3072 1045 709 678 448 36
PasI CC'CwG_GG
Cuts at: 0 2244 5988
Size: 2244 3744
PciI A'CATG_T
Cuts at: 0 2198 5988
Size: 2198 3790
PflMI CCAn_nnn'nTGG
Cuts at: 0 1908 2615 4301 4804 5988
Size: 1908 707 1686 503 1184
Fragments arranged by size:
1908 1686 1184 707 503
PfoI T'CCnGG_A
Cuts at: 0 69 2807 5384 5988
Size: 69 2738 2577 604
Fragments arranged by size:
2738 2577 604 69
PmlI CAC'GTG
Cuts at: 0 1973 5988
Size: 1973 4015
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 433 465 1618 1650 2380 2412 5988
Size: 433 32 1153 32 730 32 3576
Fragments arranged by size:
3576 1153 730 433 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 157 934 1062 2344 4314 4419 5755 5855
Size: 157 777 128 1282 1970 105 1336 100
Cuts at: 5855 5988
Size: 133
Fragments arranged by size:
1970 1336 1282 777 157 133 128 105
100
PshAI GACnn'nnGTC
Cuts at: 0 932 5988
Size: 932 5056
PspOMI G'GGCC_C
Cuts at: 0 2240 3365 5810 5988
Size: 2240 1125 2445 178
Fragments arranged by size:
2445 2240 1125 178
PspXI vC'TCGA_Gb
Cuts at: 0 921 5988
Size: 921 5067
PstI C_TGCA'G
Cuts at: 0 1080 5853 5988
Size: 1080 4773 135
Fragments arranged by size:
4773 1080 135
PvuI CG_AT'CG
Cuts at: 0 3348 5988
Size: 3348 2640
PvuII CAG'CTG
Cuts at: 0 51 619 655 676 1695 5415 5589 5988
Size: 51 568 36 21 1019 3720 174 399
Fragments arranged by size:
3720 1019 568 399 174 51 36 21
RsrII CG'GwC_CG
Cuts at: 0 292 5988
Size: 292 5696
SacI G_AGCT'C
Cuts at: 0 1391 2753 2933 5988
Size: 1391 1362 180 3055
Fragments arranged by size:
3055 1391 1362 180
SalI G'TCGA_C
Cuts at: 0 4920 5988
Size: 4920 1068
SanDI GG'GwC_CC
Cuts at: 0 4419 5755 5988
Size: 4419 1336 233
Fragments arranged by size:
4419 1336 233
SapI GCTCTTCn'nnn_
Cuts at: 0 762 1789 2758 3554 3683 5988
Size: 762 1027 969 796 129 2305
Fragments arranged by size:
2305 1027 969 796 762 129
SbfI CC_TGCA'GG
Cuts at: 0 5853 5988
Size: 5853 135
ScaI AGT'ACT
Cuts at: 0 1360 1849 4075 5988
Size: 1360 489 2226 1913
Fragments arranged by size:
2226 1913 1360 489
SexAI A'CCwGG_T
Cuts at: 0 3029 3605 5988
Size: 3029 576 2383
Fragments arranged by size:
3029 2383 576
SfcI C'TryA_G
Cuts at: 0 656 1076 1550 2101 5849 5988
Size: 656 420 474 551 3748 139
Fragments arranged by size:
3748 656 551 474 420 139
SfoI GGC'GCC
Cuts at: 0 5829 5988
Size: 5829 159
SmlI C'TyrA_G
Cuts at: 0 921 2650 2890 3061 3262 4699 5051 5988
Size: 921 1729 240 171 201 1437 352 937
Fragments arranged by size:
1729 1437 937 921 352 240 201 171
StuI AGG'CCT
Cuts at: 0 3004 3484 4348 5988
Size: 3004 480 864 1640
Fragments arranged by size:
3004 1640 864 480
StyI C'CwwG_G
Cuts at: 0 564 1283 1682 3632 4822 5250 5465 5528
Size: 564 719 399 1950 1190 428 215 63
Cuts at: 5528 5988
Size: 460
Fragments arranged by size:
1950 1190 719 564 460 428 399 215
63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 124 774 886 2427 5988
Size: 124 650 112 1541 3561
Fragments arranged by size:
3561 1541 650 124 112
TatI w'GTAC_w
Cuts at: 0 1358 1847 4073 5988
Size: 1358 489 2226 1915
Fragments arranged by size:
2226 1915 1358 489
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1378 1410 2121 2153 2328 2360 3206 3238
Size: 1378 32 711 32 175 32 846 32
Cuts at: 3238 5988
Size: 2750
Fragments arranged by size:
2750 1378 846 711 175 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 296 3436 4436 5988
Size: 296 3140 1000 1552
Fragments arranged by size:
3140 1552 1000 296
XbaI T'CTAG_A
Cuts at: 0 4836 5988
Size: 4836 1152
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 77 1235 2421 4302 5535 5988
Size: 77 1158 1186 1881 1233 453
Fragments arranged by size:
1881 1233 1186 1158 453 77
XhoI C'TCGA_G
Cuts at: 0 921 5051 5988
Size: 921 4130 937
Fragments arranged by size:
4130 937 921
XmnI GAAnn'nnTTC
Cuts at: 0 1160 2476 4243 5988
Size: 1160 1316 1767 1745
Fragments arranged by size:
1767 1745 1316 1160
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflII AflIII AgeI
AhdI AleI AloI AlwNI ApaI ApoI AvaI BaeI
BanI BanII BbeI BbsI BbvCI BcgI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrFI BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FseI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NspI PasI PciI PflMI PfoI
PmlI PpiI PpuMI PshAI PspOMI PspXI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SmlI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmnI
Enzymes that do not cut:
AatII AclI AlfI ApaLI AscI AseI AsiSI AvrII
BamHI BciVI BfrBI BglI BmtI BsaI BsiWI BsmI
BsrDI BsrGI BssHII BstBI BstZ17I EagI FspI FspAI
MfeI NheI NotI NruI NsiI PacI PmeI PsiI
PsrI SacII SfiI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI XmaI ZraI