Restriction map for
pF1KA0001
(Circular) (Six-base) MAPSORT of: pF1KA0001.seq Check: 4894 from: 1 to: 4127
pF1KA0001 4127 bp
With 182 enzymes: SgfI *
June 20, 2008 10:18 ..
AccI GT'mk_AC
Cuts at: 1165 1165
Size: 4127
Acc65I G'GTAC_C
Cuts at: 1143 1143
Size: 4127
AclI AA'CG_TT
Cuts at: 3967 3967
Size: 4127
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1952 2384 3131 3399 1952
Size: 432 747 268 2680
Fragments arranged by size:
2680 747 432 268
AflII C'TTAA_G
Cuts at: 760 760
Size: 4127
AflIII A'CryG_T
Cuts at: 377 419 1479 2583 3560 377
Size: 42 1060 1104 977 944
Fragments arranged by size:
1104 1060 977 944 42
AgeI A'CCGG_T
Cuts at: 2515 2515
Size: 4127
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 987 987
Size: 4127
AlwNI CAG_nnn'CTG
Cuts at: 1432 2999 1432
Size: 1567 2560
ApaLI G'TGCA_C
Cuts at: 2897 2897
Size: 4127
ApoI r'AATT_y
Cuts at: 774 940 1131 3480 3736 774
Size: 166 191 2349 256 1165
Fragments arranged by size:
2349 1165 256 191 166
AseI AT'TA_AT
Cuts at: 20 20
Size: 4127
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4127
AvaI C'yCGr_G
Cuts at: 1147 1147
Size: 4127
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1106 1139 1106
Size: 33 4094
BamHI G'GATC_C
Cuts at: 1152 1152
Size: 4127
BanI G'GyrC_C
Cuts at: 1143 1557 1805 1840 1143
Size: 414 248 35 3430
Fragments arranged by size:
3430 414 248 35
BanII G_rGCy'C
Cuts at: 449 1141 2171 449
Size: 692 1030 2405
Fragments arranged by size:
2405 1030 692
BbeI G_GCGC'C
Cuts at: 1561 1809 1561
Size: 248 3879
BbsI GAAGACnn'nnnn_
Cuts at: 195 195
Size: 4127
BciVI GTATCCnnnnn_n'
Cuts at: 2025 2786 4127 2025
Size: 761 1341 2025
Fragments arranged by size:
2025 1341 761
BclI T'GATC_A
Cuts at: 110 164 1647 110
Size: 54 1483 2590
Fragments arranged by size:
2590 1483 54
BglII A'GATC_T
Cuts at: 1642 3679 1642
Size: 2037 2090
BlpI GC'TnA_GC
Cuts at: 1239 1239
Size: 4127
Bme1580I G_kGCm'C
Cuts at: 260 1752 1845 2901 260
Size: 1492 93 1056 1486
Fragments arranged by size:
1492 1486 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 652 1339 1420 1755 3899 652
Size: 687 81 335 2144 880
Fragments arranged by size:
2144 880 687 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2494 2494
Size: 4127
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 119 119
Size: 4127
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 271 1303 2182 2674 2972 271
Size: 1032 879 492 298 1426
Fragments arranged by size:
1426 1032 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 399 3831 399
Size: 3432 695
BsaAI yAC'GTr
Cuts at: 255 1480 255
Size: 1225 2902
BsaBI GATnn'nnATC
Cuts at: 6 1641 3678 6
Size: 1635 2037 455
Fragments arranged by size:
2037 1635 455
BsaHI Gr'CG_yC
Cuts at: 1558 1806 2508 4004 1558
Size: 248 702 1496 1681
Fragments arranged by size:
1681 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1316 1837 2515 2789 2936 3347 1316
Size: 521 678 274 147 411 2096
Fragments arranged by size:
2096 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 117 147 117
Size: 30 4097
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 547 547
Size: 4127
BseYI C'CCAG_C
Cuts at: 259 1552 2310 2887 3383 259
Size: 1293 758 577 496 1003
Fragments arranged by size:
1293 1003 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 408 408
Size: 4127
BsiEI CG_ry'CG
Cuts at: 104 1669 1715 2923 104
Size: 1565 46 1208 1308
Fragments arranged by size:
1565 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 407 1141 1919 2109 2901 407
Size: 734 778 190 792 1633
Fragments arranged by size:
1633 792 778 734 190
Bsp1286I G_dGCh'C
Cuts at: 260 407 449 1141 1752 1845 1919 2109 2171
Size: 147 42 692 611 93 74 190 62
Cuts at: 2171 2901 260
Size: 730 1486
Fragments arranged by size:
1486 730 692 611 190 147 93 74
62 42
BspEI T'CCGG_A
Cuts at: 3347 3347
Size: 4127
BspHI T'CATG_A
Cuts at: 3303 3303
Size: 4127
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1177 1391 1693 2074 1177
Size: 214 302 381 3230
Fragments arranged by size:
3230 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2419 2473 3957 4122 50
Size: 2369 54 1484 165 55
Fragments arranged by size:
2369 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 206 2039 206
Size: 1833 2294
BsrFI r'CCGG_y
Cuts at: 2125 2306 2515 2125
Size: 181 209 3737
Fragments arranged by size:
3737 209 181
BssSI C'ACGA_G
Cuts at: 2398 2756 2398
Size: 358 3769
BstAPI GCAn_nnn'nTGC
Cuts at: 1504 1504
Size: 4127
BstBI TT'CG_AA
Cuts at: 2488 2488
Size: 4127
BstYI r'GATC_y
Cuts at: 1 338 1152 1619 1642 1977 2223 3235 3321
Size: 337 814 467 23 335 246 1012 86
Cuts at: 3321 3333 3679 1
Size: 12 346 449
Fragments arranged by size:
1012 814 467 449 346 337 335 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2077 2258 2421 2077
Size: 181 163 3783
Fragments arranged by size:
3783 181 163
BtsI GCAGTG_nn'
Cuts at: 1370 1370
Size: 4127
DraI TTT'AAA
Cuts at: 1126 1126
Size: 4127
DrdI GACnn_nn'nnGTC
Cuts at: 1833 2691 1833
Size: 858 3269
EaeI y'GGCC_r
Cuts at: 683 1712 1886 2277 2304 3649 683
Size: 1029 174 391 27 1345 1161
Fragments arranged by size:
1345 1161 1029 391 174 27
EagI C'GGCC_G
Cuts at: 1712 1712
Size: 4127
EarI CTCTTCn'nnn_
Cuts at: 412 2150 2360 412
Size: 1738 210 2179
Fragments arranged by size:
2179 1738 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2335 2645 2791 3937 2335
Size: 310 146 1146 2525
Fragments arranged by size:
2525 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1139 1139
Size: 4127
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 119 1952 2384 3131 3399 119
Size: 1833 432 747 268 847
Fragments arranged by size:
1833 847 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1266 1266
Size: 4127
EcoRI G'AATT_C
Cuts at: 774 940 1131 774
Size: 166 191 3770
Fragments arranged by size:
3770 191 166
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 762 2146 762
Size: 1384 2743
FspI TGC'GCA
Cuts at: 1908 1908
Size: 4127
HaeII r_GCGC'y
Cuts at: 1561 1809 2831 3810 1561
Size: 248 1022 979 1878
Fragments arranged by size:
1878 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 879 911 1969 1999 2001 2031 879
Size: 32 1058 30 2 30 2975
Fragments arranged by size:
2975 1058 32 30 30 2
HincII GTy'rAC
Cuts at: 417 1166 417
Size: 749 3378
KasI G'GCGC_C
Cuts at: 1557 1805 1557
Size: 248 3879
KpnI G_GTAC'C
Cuts at: 1147 1147
Size: 4127
MluI A'CGCG_T
Cuts at: 3560 3560
Size: 4127
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 751 2798 2982 751
Size: 2047 184 1896
Fragments arranged by size:
2047 1896 184
MscI TGG'CCA
Cuts at: 685 1888 685
Size: 1203 2924
MslI CAynn'nnrTG
Cuts at: 184 679 2116 2243 184
Size: 495 1437 127 2068
Fragments arranged by size:
2068 1437 495 127
MspA1I CmG'CkG
Cuts at: 372 905 1238 1552 1912 2925 3170 372
Size: 533 333 314 360 1013 245 1329
Fragments arranged by size:
1329 1013 533 360 333 314 245
NaeI GCC'GGC
Cuts at: 2308 2308
Size: 4127
NarI GG'CG_CC
Cuts at: 1558 1806 1558
Size: 248 3879
NgoMIV G'CCGG_C
Cuts at: 2306 2306
Size: 4127
NspI r_CATG'y
Cuts at: 423 1180 1680 2587 423
Size: 757 500 907 1963
Fragments arranged by size:
1963 907 757 500
PciI A'CATG_T
Cuts at: 419 2583 419
Size: 2164 1963
PflMI CCAn_nnn'nTGG
Cuts at: 692 692
Size: 4127
PmeI GTTT'AAAC
Cuts at: 1126 1126
Size: 4127
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3266 3298 3266
Size: 32 4095
PsiI TTA'TAA
Cuts at: 470 470
Size: 4127
PstI C_TGCA'G
Cuts at: 1174 1859 1174
Size: 685 3442
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4127
PvuII CAG'CTG
Cuts at: 372 905 1552 1912 372
Size: 533 647 360 2587
Fragments arranged by size:
2587 647 533 360
RsrII CG'GwC_CG
Cuts at: 2322 2322
Size: 4127
SacI G_AGCT'C
Cuts at: 1141 1141
Size: 4127
SalI G'TCGA_C
Cuts at: 1164 1164
Size: 4127
SapI GCTCTTCn'nnn_
Cuts at: 412 2150 2360 412
Size: 1738 210 2179
Fragments arranged by size:
2179 1738 210
SbfI CC_TGCA'GG
Cuts at: 1174 1174
Size: 4127
ScaI AGT'ACT
Cuts at: 3644 3644
Size: 4127
SfcI C'TryA_G
Cuts at: 33 1170 1855 2848 3039 33
Size: 1137 685 993 191 1121
Fragments arranged by size:
1137 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1559 1807 1559
Size: 248 3879
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4127
SmaI CCC'GGG
Cuts at: 1149 1149
Size: 4127
SmlI C'TyrA_G
Cuts at: 286 760 1282 2197 2689 2951 286
Size: 474 522 915 492 262 1462
Fragments arranged by size:
1462 915 522 492 474 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4127
SphI G_CATG'C
Cuts at: 1180 1680 1180
Size: 500 3627
SspI AAT'ATT
Cuts at: 576 576
Size: 4127
StuI AGG'CCT
Cuts at: 356 356
Size: 4127
StyI C'CwwG_G
Cuts at: 342 357 1261 1615 342
Size: 15 904 354 2854
Fragments arranged by size:
2854 904 354 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 774 904 1710 1843 2302 2511 774
Size: 130 806 133 459 209 2390
Fragments arranged by size:
2390 806 459 209 133 130
TatI w'GTAC_w
Cuts at: 191 3642 191
Size: 3451 676
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 117 149 3644 3676 117
Size: 32 3495 32 568
Fragments arranged by size:
3495 568 32 32
Tth111I GACn'n_nGTC
Cuts at: 1924 1924
Size: 4127
XbaI T'CTAG_A
Cuts at: 1158 1158
Size: 4127
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1622 1622
Size: 4127
XmaI C'CCGG_G
Cuts at: 1147 1147
Size: 4127
XmnI GAAnn'nnTTC
Cuts at: 940 940
Size: 4127
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI
PflMI PmeI PpiI PsiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AloI ApaI AscI
AvrII BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I BtgI ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PfoI PmlI PpuMI PshAI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SwaI XhoI ZraI