Restriction map for
pF1KA0006
(Circular) (Six-base) MAPSORT of: pF1KA0006.seq Check: 2116 from: 1 to: 5441
pF1KA0006 5441 bp
With 182 enzymes: SgfI *
October 24, 2019 20:49 ..
AccI GT'mk_AC
Cuts at: 1065 1887 2479 1065
Size: 822 592 4027
Fragments arranged by size:
4027 822 592
Acc65I G'GTAC_C
Cuts at: 1514 2457 1514
Size: 943 4498
AclI AA'CG_TT
Cuts at: 5281 5281
Size: 5441
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 314 871 1109 2311 3266 3698 4445 4713 314
Size: 557 238 1202 955 432 747 268 1042
Fragments arranged by size:
1202 1042 955 747 557 432 268 238
AflII C'TTAA_G
Cuts at: 909 909
Size: 5441
AflIII A'CryG_T
Cuts at: 2793 3897 4874 2793
Size: 1104 977 3360
Fragments arranged by size:
3360 1104 977
AgeI A'CCGG_T
Cuts at: 3829 3829
Size: 5441
AlwNI CAG_nnn'CTG
Cuts at: 1401 2746 4313 1401
Size: 1345 1567 2529
Fragments arranged by size:
2529 1567 1345
ApaLI G'TGCA_C
Cuts at: 4211 4211
Size: 5441
ApoI r'AATT_y
Cuts at: 1990 2118 2445 4794 5050 1990
Size: 128 327 2349 256 2381
Fragments arranged by size:
2381 2349 327 256 128
AseI AT'TA_AT
Cuts at: 20 20
Size: 5441
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5441
AvaI C'yCGr_G
Cuts at: 1835 2461 1835
Size: 626 4815
BamHI G'GATC_C
Cuts at: 282 2466 282
Size: 2184 3257
BanI G'GyrC_C
Cuts at: 1514 2457 2871 3119 3154 1514
Size: 943 414 248 35 3801
Fragments arranged by size:
3801 943 414 248 35
BanII G_rGCy'C
Cuts at: 1198 2455 3485 1198
Size: 1257 1030 3154
Fragments arranged by size:
3154 1257 1030
BbeI G_GCGC'C
Cuts at: 2875 3123 2875
Size: 248 5193
BbsI GAAGACnn'nnnn_
Cuts at: 2014 2014
Size: 5441
BbvCI CC'TCA_GC
Cuts at: 1225 1225
Size: 5441
BciVI GTATCCnnnnn_n'
Cuts at: 1213 1287 3339 4100 5441 1213
Size: 74 2052 761 1341 1213
Fragments arranged by size:
2052 1341 1213 761 74
BclI T'GATC_A
Cuts at: 239 1745 2961 239
Size: 1506 1216 2719
Fragments arranged by size:
2719 1506 1216
BglII A'GATC_T
Cuts at: 1365 2956 4993 1365
Size: 1591 2037 1813
Fragments arranged by size:
2037 1813 1591
BlpI GC'TnA_GC
Cuts at: 2553 2553
Size: 5441
Bme1580I G_kGCm'C
Cuts at: 3066 3159 4215 3066
Size: 93 1056 4292
Fragments arranged by size:
4292 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 731 973 2653 2734 3069 5213 731
Size: 242 1680 81 335 2144 959
Fragments arranged by size:
2144 1680 959 335 242 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3808 3808
Size: 5441
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1267 1408 1267
Size: 141 5300
Bpu10I CC'TnA_GC
Cuts at: 1225 1225
Size: 5441
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2124 2617 3496 3988 4286 2124
Size: 493 879 492 298 3279
Fragments arranged by size:
3279 879 493 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 765 5145 765
Size: 4380 1061
BsaAI yAC'GTr
Cuts at: 2794 2794
Size: 5441
BsaBI GATnn'nnATC
Cuts at: 6 1298 2955 4992 6
Size: 1292 1657 2037 455
Fragments arranged by size:
2037 1657 1292 455
BsaHI Gr'CG_yC
Cuts at: 2872 3120 3822 5318 2872
Size: 248 702 1496 2995
Fragments arranged by size:
2995 1496 702 248
BsaWI w'CCGG_w
Cuts at: 186 1825 2630 3151 3829 4103 4250 4661 186
Size: 1639 805 521 678 274 147 411 966
Fragments arranged by size:
1639 966 805 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1866 1896 1866
Size: 30 5411
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 205 1522 1844 2044 205
Size: 1317 322 200 3602
Fragments arranged by size:
3602 1317 322 200
BseYI C'CCAG_C
Cuts at: 2866 3624 4201 4697 2866
Size: 758 577 496 3610
Fragments arranged by size:
3610 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1501 1632 1880 2091 1501
Size: 131 248 211 4851
Fragments arranged by size:
4851 248 211 131
BsiEI CG_ry'CG
Cuts at: 104 2983 3029 4237 104
Size: 2879 46 1208 1308
Fragments arranged by size:
2879 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1142 1806 2455 3233 3423 4215 1142
Size: 664 649 778 190 792 2368
Fragments arranged by size:
2368 792 778 664 649 190
BsmI GAATG_Cn'
Cuts at: 1336 1336
Size: 5441
Bsp1286I G_dGCh'C
Cuts at: 1142 1198 1806 2455 3066 3159 3233 3423 3485
Size: 56 608 649 611 93 74 190 62
Cuts at: 3485 4215 1142
Size: 730 2368
Fragments arranged by size:
2368 730 649 611 608 190 93 74
62 56
BspEI T'CCGG_A
Cuts at: 186 4661 186
Size: 4475 966
BspHI T'CATG_A
Cuts at: 4617 4617
Size: 5441
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1764 1902 2491 2705 3007 3388 1764
Size: 138 589 214 302 381 3817
Fragments arranged by size:
3817 589 381 302 214 138
BsrBI CCG'CTC
Cuts at: 50 1512 3733 3787 5271 5436 50
Size: 1462 2221 54 1484 165 55
Fragments arranged by size:
2221 1484 1462 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1363 2323 3353 1363
Size: 960 1030 3451
Fragments arranged by size:
3451 1030 960
BsrFI r'CCGG_y
Cuts at: 3439 3620 3829 3439
Size: 181 209 5051
Fragments arranged by size:
5051 209 181
BssSI C'ACGA_G
Cuts at: 677 3712 4070 677
Size: 3035 358 2048
Fragments arranged by size:
3035 2048 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2818 2818
Size: 5441
BstBI TT'CG_AA
Cuts at: 3802 3802
Size: 5441
BstYI r'GATC_y
Cuts at: 1 282 1365 2466 2933 2956 3291 3537 4549
Size: 281 1083 1101 467 23 335 246 1012
Cuts at: 4549 4635 4647 4993 1
Size: 86 12 346 449
Fragments arranged by size:
1101 1083 1012 467 449 346 335 281
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2180 2180
Size: 5441
BtgI C'CryG_G
Cuts at: 1870 2133 1870
Size: 263 5178
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3391 3572 3735 3391
Size: 181 163 5097
Fragments arranged by size:
5097 181 163
BtsI GCAGTG_nn'
Cuts at: 2684 2684
Size: 5441
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 545 580 545
Size: 35 5406
DraI TTT'AAA
Cuts at: 200 1089 2440 200
Size: 889 1351 3201
Fragments arranged by size:
3201 1351 889
DrdI GACnn_nn'nnGTC
Cuts at: 3147 4005 3147
Size: 858 4583
EaeI y'GGCC_r
Cuts at: 2220 3026 3200 3591 3618 4963 2220
Size: 806 174 391 27 1345 2698
Fragments arranged by size:
2698 1345 806 391 174 27
EagI C'GGCC_G
Cuts at: 3026 3026
Size: 5441
EarI CTCTTCn'nnn_
Cuts at: 480 899 1369 2063 2180 3464 3674 480
Size: 419 470 694 117 1284 210 2247
Fragments arranged by size:
2247 1284 694 470 419 210 117
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3649 3959 4105 5251 3649
Size: 310 146 1146 3839
Fragments arranged by size:
3839 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2453 2453
Size: 5441
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 314 871 1109 1267 1408 2311 3266 3698 4445
Size: 557 238 158 141 903 955 432 747
Cuts at: 4445 4713 314
Size: 268 1042
Fragments arranged by size:
1042 955 903 747 557 432 268 238
158 141
EcoO109I rG'GnC_Cy
Cuts at: 1753 1840 2349 2580 1753
Size: 87 509 231 4614
Fragments arranged by size:
4614 509 231 87
EcoRI G'AATT_C
Cuts at: 2445 2445
Size: 5441
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3460 3460
Size: 5441
FspI TGC'GCA
Cuts at: 3222 3222
Size: 5441
HaeII r_GCGC'y
Cuts at: 2875 3123 4145 5124 2875
Size: 248 1022 979 3192
Fragments arranged by size:
3192 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 433 465 628 660 940 972 1927 1959 3283
Size: 32 163 32 280 32 955 32 1324
Cuts at: 3283 3313 3315 3345 433
Size: 30 2 30 2529
Fragments arranged by size:
2529 1324 955 280 163 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 426 2480 426
Size: 2054 3387
KasI G'GCGC_C
Cuts at: 2871 3119 2871
Size: 248 5193
KpnI G_GTAC'C
Cuts at: 1518 2461 1518
Size: 943 4498
MluI A'CGCG_T
Cuts at: 4874 4874
Size: 5441
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1142 1706 4112 4296 1142
Size: 564 2406 184 2287
Fragments arranged by size:
2406 2287 564 184
MscI TGG'CCA
Cuts at: 3202 3202
Size: 5441
MslI CAynn'nnrTG
Cuts at: 1154 3430 3557 1154
Size: 2276 127 3038
Fragments arranged by size:
3038 2276 127
MspA1I CmG'CkG
Cuts at: 1129 1386 1734 2059 2552 2866 3226 4239 4484
Size: 257 348 325 493 314 360 1013 245
Cuts at: 4484 1129
Size: 2086
Fragments arranged by size:
2086 1013 493 360 348 325 314 257
245
NaeI GCC'GGC
Cuts at: 3622 3622
Size: 5441
NarI GG'CG_CC
Cuts at: 2872 3120 2872
Size: 248 5193
NcoI C'CATG_G
Cuts at: 2133 2133
Size: 5441
NdeI CA'TA_TG
Cuts at: 1286 1286
Size: 5441
NgoMIV G'CCGG_C
Cuts at: 3620 3620
Size: 5441
NspI r_CATG'y
Cuts at: 1496 2494 2994 3901 1496
Size: 998 500 907 3036
Fragments arranged by size:
3036 998 907 500
PciI A'CATG_T
Cuts at: 3897 3897
Size: 5441
PflMI CCAn_nnn'nTGG
Cuts at: 1014 1014
Size: 5441
PfoI T'CCnGG_A
Cuts at: 854 1715 854
Size: 861 4580
PmeI GTTT'AAAC
Cuts at: 2440 2440
Size: 5441
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4580 4612 4580
Size: 32 5409
PpuMI rG'GwC_Cy
Cuts at: 1753 1840 2349 1753
Size: 87 509 4845
Fragments arranged by size:
4845 509 87
PsiI TTA'TAA
Cuts at: 1864 1931 1864
Size: 67 5374
PstI C_TGCA'G
Cuts at: 923 2488 3173 923
Size: 1565 685 3191
Fragments arranged by size:
3191 1565 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5441
PvuII CAG'CTG
Cuts at: 1129 1386 1734 2059 2866 3226 1129
Size: 257 348 325 807 360 3344
Fragments arranged by size:
3344 807 360 348 325 257
RsrII CG'GwC_CG
Cuts at: 3636 3636
Size: 5441
SacI G_AGCT'C
Cuts at: 2455 2455
Size: 5441
SalI G'TCGA_C
Cuts at: 2478 2478
Size: 5441
SapI GCTCTTCn'nnn_
Cuts at: 3464 3674 3464
Size: 210 5231
SbfI CC_TGCA'GG
Cuts at: 2488 2488
Size: 5441
ScaI AGT'ACT
Cuts at: 411 942 2145 4958 411
Size: 531 1203 2813 894
Fragments arranged by size:
2813 1203 894 531
SfcI C'TryA_G
Cuts at: 33 919 2484 3169 4162 4353 33
Size: 886 1565 685 993 191 1121
Fragments arranged by size:
1565 1121 993 886 685 191
SfoI GGC'GCC
Cuts at: 2873 3121 2873
Size: 248 5193
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5441
SmaI CCC'GGG
Cuts at: 2463 2463
Size: 5441
SmlI C'TyrA_G
Cuts at: 909 2139 2596 3511 4003 4265 909
Size: 1230 457 915 492 262 2085
Fragments arranged by size:
2085 1230 915 492 457 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5441
SphI G_CATG'C
Cuts at: 2494 2994 2494
Size: 500 4941
SspI AAT'ATT
Cuts at: 359 359
Size: 5441
StyI C'CwwG_G
Cuts at: 398 1013 1844 2133 2575 2929 398
Size: 615 831 289 442 354 2910
Fragments arranged by size:
2910 831 615 442 354 289
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3024 3157 3616 3825 3024
Size: 133 459 209 4640
Fragments arranged by size:
4640 459 209 133
TatI w'GTAC_w
Cuts at: 409 940 2053 2143 4956 409
Size: 531 1113 90 2813 894
Fragments arranged by size:
2813 1113 894 531 90
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1598 1630 4958 4990 1598
Size: 32 3328 32 2049
Fragments arranged by size:
3328 2049 32 32
Tth111I GACn'n_nGTC
Cuts at: 2278 3238 2278
Size: 960 4481
XbaI T'CTAG_A
Cuts at: 2472 2472
Size: 5441
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2936 2936
Size: 5441
XmaI C'CCGG_G
Cuts at: 2461 2461
Size: 5441
XmnI GAAnn'nnTTC
Cuts at: 1412 2154 1412
Size: 742 4699
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NspI PciI PflMI PfoI
PmeI PpiI PpuMI PsiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BcgI BfrBI BglI BmgBI BmtI
BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I ClaI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NheI NotI NruI NsiI PacI PasI PmlI PshAI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI StuI SwaI XhoI ZraI