Restriction map for
pF1KA0008
(Circular) (Six-base) MAPSORT of: pF1KA0008.seq Check: 7035 from: 1 to: 5651
pF1KA0008 5651 bp
With 182 enzymes: SgfI *
June 20, 2008 10:18 ..
AccI GT'mk_AC
Cuts at: 132 2689 132
Size: 2557 3094
Acc65I G'GTAC_C
Cuts at: 2667 2667
Size: 5651
AclI AA'CG_TT
Cuts at: 5491 5491
Size: 5651
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1087 2018 3476 3908 4261 4923 1087
Size: 931 1458 432 353 662 1815
Fragments arranged by size:
1815 1458 931 662 432 353
AflIII A'CryG_T
Cuts at: 1284 2130 3003 4803 5084 1284
Size: 846 873 1800 281 1851
Fragments arranged by size:
1851 1800 873 846 281
AhdI GACnn_n'nnGTC
Cuts at: 1987 1987
Size: 5651
AleI CACnn'nnGTG
Cuts at: 1391 1391
Size: 5651
AlwNI CAG_nnn'CTG
Cuts at: 1056 1429 1900 2956 4394 1056
Size: 373 471 1056 1438 2313
Fragments arranged by size:
2313 1438 1056 471 373
ApaLI G'TGCA_C
Cuts at: 4489 4489
Size: 5651
ApoI r'AATT_y
Cuts at: 281 921 1020 1181 1317 1675 2339 2370 2462
Size: 640 99 161 136 358 664 31 92
Cuts at: 2462 2641 2655 5004 5260 281
Size: 179 14 2349 256 672
Fragments arranged by size:
2349 672 664 640 358 256 179 161
136 99 92 31 14
AseI AT'TA_AT
Cuts at: 20 2318 20
Size: 2298 3353
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5651
AvaI C'yCGr_G
Cuts at: 2671 2671
Size: 5651
BamHI G'GATC_C
Cuts at: 2676 2676
Size: 5651
BanI G'GyrC_C
Cuts at: 831 2667 3081 3329 3364 831
Size: 1836 414 248 35 3118
Fragments arranged by size:
3118 1836 414 248 35
BanII G_rGCy'C
Cuts at: 2665 3695 2665
Size: 1030 4621
BbeI G_GCGC'C
Cuts at: 3085 3333 3085
Size: 248 5403
BbsI GAAGACnn'nnnn_
Cuts at: 104 852 1075 2146 104
Size: 748 223 1071 3609
Fragments arranged by size:
3609 1071 748 223
BciVI GTATCCnnnnn_n'
Cuts at: 3549 4605 5651 3549
Size: 1056 1046 3549
Fragments arranged by size:
3549 1056 1046
BclI T'GATC_A
Cuts at: 369 1688 3171 369
Size: 1319 1483 2849
Fragments arranged by size:
2849 1483 1319
BglII A'GATC_T
Cuts at: 1500 1620 3166 5203 1500
Size: 120 1546 2037 1948
Fragments arranged by size:
2037 1948 1546 120
BlpI GC'TnA_GC
Cuts at: 527 1379 2763 527
Size: 852 1384 3415
Fragments arranged by size:
3415 1384 852
Bme1580I G_kGCm'C
Cuts at: 3276 3369 4493 3276
Size: 93 1124 4434
Fragments arranged by size:
4434 1124 93
BmgBI CAC'GTC
Cuts at: 710 710
Size: 5651
BmrI ACTGGGnnnn_n'
Cuts at: 1118 2863 2944 3279 5423 1118
Size: 1745 81 335 2144 1346
Fragments arranged by size:
2144 1745 1346 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 740 2303 4018 740
Size: 1563 1715 2373
Fragments arranged by size:
2373 1715 1563
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1402 2581 1402
Size: 1179 4472
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 293 722 1151 1717 2827 3706 4420 4718 293
Size: 429 429 566 1110 879 714 298 1226
Fragments arranged by size:
1226 1110 879 714 566 429 429 298
BsaI GGTCTCn'nnnn_
Cuts at: 5355 5355
Size: 5651
BsaAI yAC'GTr
Cuts at: 3004 3004
Size: 5651
BsaBI GATnn'nnATC
Cuts at: 6 3165 5202 6
Size: 3159 2037 455
Fragments arranged by size:
3159 2037 455
BsaHI Gr'CG_yC
Cuts at: 3082 3330 4032 5528 3082
Size: 248 702 1496 3205
Fragments arranged by size:
3205 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2840 3361 4039 4450 4597 4871 2840
Size: 521 678 411 147 274 3620
Fragments arranged by size:
3620 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 371 401 1122 1152 2630 2660 371
Size: 30 721 30 1478 30 3362
Fragments arranged by size:
3362 1478 721 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2580 2580
Size: 5651
BseYI C'CCAG_C
Cuts at: 3076 3834 4499 4907 3076
Size: 758 665 408 3820
Fragments arranged by size:
3820 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 712 712
Size: 5651
BsiEI CG_ry'CG
Cuts at: 104 3193 3239 4469 104
Size: 3089 46 1230 1286
Fragments arranged by size:
3089 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 2665 3443 3633 4493 2665
Size: 778 190 860 3823
Fragments arranged by size:
3823 860 778 190
BsmI GAATG_Cn'
Cuts at: 519 2072 2512 519
Size: 1553 440 3658
Fragments arranged by size:
3658 1553 440
Bsp1286I G_dGCh'C
Cuts at: 2665 3276 3369 3443 3633 3695 4493 2665
Size: 611 93 74 190 62 798 3823
Fragments arranged by size:
3823 798 611 190 93 74 62
BspHI T'CATG_A
Cuts at: 4083 4083
Size: 5651
BspMI ACCTGCnnnn'nnnn_
Cuts at: 741 858 2701 2915 3217 3598 741
Size: 117 1843 214 302 381 2794
Fragments arranged by size:
2794 1843 381 302 214 117
BsrBI CCG'CTC
Cuts at: 50 3943 3997 5481 5646 50
Size: 3893 54 1484 165 55
Fragments arranged by size:
3893 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 356 1446 1859 3563 356
Size: 1090 413 1704 2444
Fragments arranged by size:
2444 1704 1090 413
BsrFI r'CCGG_y
Cuts at: 2104 3649 3830 2104
Size: 1545 181 3925
Fragments arranged by size:
3925 1545 181
BsrGI T'GTAC_A
Cuts at: 1615 1615
Size: 5651
BssSI C'ACGA_G
Cuts at: 3922 4630 3922
Size: 708 4943
BstAPI GCAn_nnn'nTGC
Cuts at: 3028 3028
Size: 5651
BstBI TT'CG_AA
Cuts at: 4012 4012
Size: 5651
BstEII G'GTnAC_C
Cuts at: 2605 2605
Size: 5651
BstYI r'GATC_y
Cuts at: 1 1500 1620 2676 3143 3166 3501 3747 4053
Size: 1499 120 1056 467 23 335 246 306
Cuts at: 4053 4065 4151 5203 1
Size: 12 86 1052 449
Fragments arranged by size:
1499 1056 1052 467 449 335 306 246
120 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1034 1034
Size: 5651
BtgI C'CryG_G
Cuts at: 1389 1389
Size: 5651
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3601 3782 3945 3601
Size: 181 163 5307
Fragments arranged by size:
5307 181 163
BtsI GCAGTG_nn'
Cuts at: 2894 2894
Size: 5651
DraI TTT'AAA
Cuts at: 462 1145 1292 1322 2650 462
Size: 683 147 30 1328 3463
Fragments arranged by size:
3463 1328 683 147 30
DrdI GACnn_nn'nnGTC
Cuts at: 3357 4701 3357
Size: 1344 4307
EaeI y'GGCC_r
Cuts at: 3236 3410 3801 3828 5173 3236
Size: 174 391 27 1345 3714
Fragments arranged by size:
3714 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3236 3236
Size: 5651
EarI CTCTTCn'nnn_
Cuts at: 1998 2294 3674 3884 1998
Size: 296 1380 210 3765
Fragments arranged by size:
3765 1380 296 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3859 4601 4747 5461 3859
Size: 742 146 714 4049
Fragments arranged by size:
4049 742 714 146
EcoICRI GAG'CTC
Cuts at: 2663 2663
Size: 5651
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1087 1402 2018 2581 3476 3908 4261 4923 1087
Size: 315 616 563 895 432 353 662 1815
Fragments arranged by size:
1815 895 662 616 563 432 353 315
EcoNI CCTnn'n_nnAGG
Cuts at: 1540 1540
Size: 5651
EcoO109I rG'GnC_Cy
Cuts at: 1258 2002 2790 1258
Size: 744 788 4119
Fragments arranged by size:
4119 788 744
EcoRI G'AATT_C
Cuts at: 281 921 1020 2370 2655 281
Size: 640 99 1350 285 3277
Fragments arranged by size:
3277 1350 640 285 99
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 668 1041 2262 3670 668
Size: 373 1221 1408 2649
Fragments arranged by size:
2649 1408 1221 373
FspI TGC'GCA
Cuts at: 3432 3432
Size: 5651
HaeII r_GCGC'y
Cuts at: 3085 3333 4563 5334 3085
Size: 248 1230 771 3402
Fragments arranged by size:
3402 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 289 321 1973 2005 2248 2280 2556 2588 3493
Size: 32 1652 32 243 32 276 32 905
Cuts at: 3493 3523 3525 3555 289
Size: 30 2 30 2385
Fragments arranged by size:
2385 1652 905 276 243 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 133 2037 2690 133
Size: 1904 653 3094
Fragments arranged by size:
3094 1904 653
KasI G'GCGC_C
Cuts at: 3081 3329 3081
Size: 248 5403
KpnI G_GTAC'C
Cuts at: 2671 2671
Size: 5651
MluI A'CGCG_T
Cuts at: 5084 5084
Size: 5651
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 289 657 4410 4594 289
Size: 368 3753 184 1346
Fragments arranged by size:
3753 1346 368 184
MscI TGG'CCA
Cuts at: 3412 3412
Size: 5651
MslI CAynn'nnrTG
Cuts at: 1194 1391 3640 3767 1194
Size: 197 2249 127 3078
Fragments arranged by size:
3078 2249 197 127
MspA1I CmG'CkG
Cuts at: 2559 2762 3076 3436 4220 4465 2559
Size: 203 314 360 784 245 3745
Fragments arranged by size:
3745 784 360 314 245 203
NaeI GCC'GGC
Cuts at: 3832 3832
Size: 5651
NarI GG'CG_CC
Cuts at: 3082 3330 3082
Size: 248 5403
NcoI C'CATG_G
Cuts at: 1389 1389
Size: 5651
NgoMIV G'CCGG_C
Cuts at: 3830 3830
Size: 5651
NspI r_CATG'y
Cuts at: 1288 2134 2584 2704 3204 4807 1288
Size: 846 450 120 500 1603 2132
Fragments arranged by size:
2132 1603 846 500 450 120
PciI A'CATG_T
Cuts at: 1284 2130 4803 1284
Size: 846 2673 2132
Fragments arranged by size:
2673 2132 846
PflMI CCAn_nnn'nTGG
Cuts at: 1060 1060
Size: 5651
PfoI T'CCnGG_A
Cuts at: 2196 2196
Size: 5651
PmeI GTTT'AAAC
Cuts at: 2650 2650
Size: 5651
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4097 4129 4097
Size: 32 5619
PpuMI rG'GwC_Cy
Cuts at: 1258 1258
Size: 5651
PstI C_TGCA'G
Cuts at: 2542 2698 3383 2542
Size: 156 685 4810
Fragments arranged by size:
4810 685 156
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5651
PvuII CAG'CTG
Cuts at: 2559 3076 3436 2559
Size: 517 360 4774
Fragments arranged by size:
4774 517 360
RsrII CG'GwC_CG
Cuts at: 3846 3846
Size: 5651
SacI G_AGCT'C
Cuts at: 2665 2665
Size: 5651
SalI G'TCGA_C
Cuts at: 131 2688 131
Size: 2557 3094
SapI GCTCTTCn'nnn_
Cuts at: 3674 3884 3674
Size: 210 5441
SbfI CC_TGCA'GG
Cuts at: 2698 2698
Size: 5651
ScaI AGT'ACT
Cuts at: 153 5168 153
Size: 5015 636
SexAI A'CCwGG_T
Cuts at: 636 2526 636
Size: 1890 3761
SfcI C'TryA_G
Cuts at: 33 2538 2694 3379 4347 4538 33
Size: 2505 156 685 968 191 1146
Fragments arranged by size:
2505 1146 968 685 191 156
SfoI GGC'GCC
Cuts at: 3083 3331 3083
Size: 248 5403
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5651
SmaI CCC'GGG
Cuts at: 2673 2673
Size: 5651
SmlI C'TyrA_G
Cuts at: 308 737 1166 1696 2806 3721 4435 4697 308
Size: 429 429 530 1110 915 714 262 1262
Fragments arranged by size:
1262 1110 915 714 530 429 429 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5651
SphI G_CATG'C
Cuts at: 2704 3204 2704
Size: 500 5151
SspI AAT'ATT
Cuts at: 458 458
Size: 5651
StyI C'CwwG_G
Cuts at: 269 343 1389 2785 3139 269
Size: 74 1046 1396 354 2781
Fragments arranged by size:
2781 1396 1046 354 74
SwaI ATTT'AAAT
Cuts at: 1322 1322
Size: 5651
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1137 1697 3234 3367 3826 4035 1137
Size: 560 1537 133 459 209 2753
Fragments arranged by size:
2753 1537 560 459 209 133
TatI w'GTAC_w
Cuts at: 151 1615 5166 151
Size: 1464 3551 636
Fragments arranged by size:
3551 1464 636
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1122 1154 1693 1725 5168 5200 1122
Size: 32 539 32 3443 32 1573
Fragments arranged by size:
3443 1573 539 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 638 3448 638
Size: 2810 2841
XbaI T'CTAG_A
Cuts at: 2682 2682
Size: 5651
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 585 3146 585
Size: 2561 3090
XmaI C'CCGG_G
Cuts at: 2671 2671
Size: 5651
XmnI GAAnn'nnTTC
Cuts at: 1024 1024
Size: 5651
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AhdI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PfoI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StyI SwaI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmtI
Bpu10I BsiWI BsmBI BspEI BssHII BstXI BstZ17I ClaI
CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PmlI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SfiI
SgrAI SnaBI SrfI StuI XhoI ZraI