Restriction map for
pF1KA0012
(Circular) (Six-base) MAPSORT of: pF1KA0012.seq Check: 8860 from: 1 to: 5471
pF1KA0012 5471 bp
With 182 enzymes: SgfI *
June 20, 2008 10:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2170 2170
Size: 5471
AccI GT'mk_AC
Cuts at: 2509 2509
Size: 5471
Acc65I G'GTAC_C
Cuts at: 2487 2487
Size: 5471
AclI AA'CG_TT
Cuts at: 5311 5311
Size: 5471
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 136 194 957 3296 3728 4475 4743 136
Size: 58 763 2339 432 747 268 864
Fragments arranged by size:
2339 864 763 747 432 268 58
AflII C'TTAA_G
Cuts at: 2421 2421
Size: 5471
AflIII A'CryG_T
Cuts at: 1820 2823 3927 4904 1820
Size: 1003 1104 977 2387
Fragments arranged by size:
2387 1104 1003 977
AgeI A'CCGG_T
Cuts at: 3859 3859
Size: 5471
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 625 625
Size: 5471
AlwNI CAG_nnn'CTG
Cuts at: 1108 2776 4343 1108
Size: 1668 1567 2236
Fragments arranged by size:
2236 1668 1567
ApaLI G'TGCA_C
Cuts at: 4241 4241
Size: 5471
ApoI r'AATT_y
Cuts at: 184 376 532 709 882 1095 1329 1708 2475
Size: 192 156 177 173 213 234 379 767
Cuts at: 2475 4824 5080 184
Size: 2349 256 575
Fragments arranged by size:
2349 767 575 379 256 234 213 192
177 173 156
AseI AT'TA_AT
Cuts at: 20 2435 20
Size: 2415 3056
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5471
AvaI C'yCGr_G
Cuts at: 2491 2491
Size: 5471
BamHI G'GATC_C
Cuts at: 896 2496 896
Size: 1600 3871
BanI G'GyrC_C
Cuts at: 1214 2225 2487 2901 3149 3184 1214
Size: 1011 262 414 248 35 3501
Fragments arranged by size:
3501 1011 414 262 248 35
BanII G_rGCy'C
Cuts at: 2485 3515 2485
Size: 1030 4441
BbeI G_GCGC'C
Cuts at: 2905 3153 2905
Size: 248 5223
BbsI GAAGACnn'nnnn_
Cuts at: 650 1391 650
Size: 741 4730
BciVI GTATCCnnnnn_n'
Cuts at: 3369 4130 5471 3369
Size: 761 1341 3369
Fragments arranged by size:
3369 1341 761
BclI T'GATC_A
Cuts at: 1592 1599 2991 1592
Size: 7 1392 4072
Fragments arranged by size:
4072 1392 7
BfrBI ATG'CAT
Cuts at: 481 2023 481
Size: 1542 3929
BglII A'GATC_T
Cuts at: 2986 5023 2986
Size: 2037 3434
BlpI GC'TnA_GC
Cuts at: 2583 2583
Size: 5471
Bme1580I G_kGCm'C
Cuts at: 1217 2045 3096 3189 4245 1217
Size: 828 1051 93 1056 2443
Fragments arranged by size:
2443 1056 1051 828 93
BmrI ACTGGGnnnn_n'
Cuts at: 2683 2764 3099 5243 2683
Size: 81 335 2144 2911
Fragments arranged by size:
2911 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3838 3838
Size: 5471
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 886 1996 886
Size: 1110 4361
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 439 2647 3526 4018 4316 439
Size: 2208 879 492 298 1594
Fragments arranged by size:
2208 1594 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 217 5175 217
Size: 4958 513
BsaAI yAC'GTr
Cuts at: 2824 2824
Size: 5471
BsaBI GATnn'nnATC
Cuts at: 6 2985 5022 6
Size: 2979 2037 455
Fragments arranged by size:
2979 2037 455
BsaHI Gr'CG_yC
Cuts at: 2902 3150 3852 5348 2902
Size: 248 702 1496 3025
Fragments arranged by size:
3025 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2660 3181 3859 4133 4280 4691 2660
Size: 521 678 274 147 411 3440
Fragments arranged by size:
3440 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1697 1727 1697
Size: 30 5441
BseYI C'CCAG_C
Cuts at: 2896 3654 4231 4727 2896
Size: 758 577 496 3640
Fragments arranged by size:
3640 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 667 667
Size: 5471
BsiEI CG_ry'CG
Cuts at: 104 3013 3059 4267 104
Size: 2909 46 1208 1308
Fragments arranged by size:
2909 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 550 2485 3263 3453 4245 550
Size: 1935 778 190 792 1776
Fragments arranged by size:
1935 1776 792 778 190
BsmI GAATG_Cn'
Cuts at: 1484 1484
Size: 5471
Bsp1286I G_dGCh'C
Cuts at: 550 1217 2045 2485 3096 3189 3263 3453 3515
Size: 667 828 440 611 93 74 190 62
Cuts at: 3515 4245 550
Size: 730 1776
Fragments arranged by size:
1776 828 730 667 611 440 190 93
74 62
BspEI T'CCGG_A
Cuts at: 4691 4691
Size: 5471
BspHI T'CATG_A
Cuts at: 2112 2265 4647 2112
Size: 153 2382 2936
Fragments arranged by size:
2936 2382 153
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2170 2521 2735 3037 3418 2170
Size: 351 214 302 381 4223
Fragments arranged by size:
4223 381 351 302 214
BsrBI CCG'CTC
Cuts at: 50 3763 3817 5301 5466 50
Size: 3713 54 1484 165 55
Fragments arranged by size:
3713 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3383 3383
Size: 5471
BsrFI r'CCGG_y
Cuts at: 3469 3650 3859 3469
Size: 181 209 5081
Fragments arranged by size:
5081 209 181
BssSI C'ACGA_G
Cuts at: 3742 4100 3742
Size: 358 5113
BstAPI GCAn_nnn'nTGC
Cuts at: 487 2848 487
Size: 2361 3110
BstBI TT'CG_AA
Cuts at: 1078 3832 1078
Size: 2754 2717
BstXI CCAn_nnnn'nTGG
Cuts at: 1878 1878
Size: 5471
BstYI r'GATC_y
Cuts at: 1 896 1920 2272 2496 2963 2986 3321 3567
Size: 895 1024 352 224 467 23 335 246
Cuts at: 3567 4579 4665 4677 5023 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1024 1012 895 467 449 352 346 335
246 224 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 650 650
Size: 5471
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1049 3421 3602 3765 1049
Size: 2372 181 163 2755
Fragments arranged by size:
2755 2372 181 163
BtsI GCAGTG_nn'
Cuts at: 2714 2714
Size: 5471
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1114 1149 1114
Size: 35 5436
DraI TTT'AAA
Cuts at: 1391 2470 1391
Size: 1079 4392
DraIII CAC_nnn'GTG
Cuts at: 690 690
Size: 5471
DrdI GACnn_nn'nnGTC
Cuts at: 3177 4035 3177
Size: 858 4613
EaeI y'GGCC_r
Cuts at: 2357 3056 3230 3621 3648 4993 2357
Size: 699 174 391 27 1345 2835
Fragments arranged by size:
2835 1345 699 391 174 27
EagI C'GGCC_G
Cuts at: 3056 3056
Size: 5471
EarI CTCTTCn'nnn_
Cuts at: 2167 3494 3704 2167
Size: 1327 210 3934
Fragments arranged by size:
3934 1327 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3679 3989 4135 5281 3679
Size: 310 146 1146 3869
Fragments arranged by size:
3869 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2483 2483
Size: 5471
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 136 194 886 957 1996 3296 3728 4475 4743
Size: 58 692 71 1039 1300 432 747 268
Cuts at: 4743 136
Size: 864
Fragments arranged by size:
1300 1039 864 747 692 432 268 71
58
EcoNI CCTnn'n_nnAGG
Cuts at: 1807 1807
Size: 5471
EcoO109I rG'GnC_Cy
Cuts at: 654 2610 654
Size: 1956 3515
EcoRI G'AATT_C
Cuts at: 709 882 1329 2475 709
Size: 173 447 1146 3705
Fragments arranged by size:
3705 1146 447 173
EcoRV GAT'ATC
Cuts at: 363 363
Size: 5471
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2205 3490 2205
Size: 1285 4186
FspI TGC'GCA
Cuts at: 3252 3252
Size: 5471
HaeII r_GCGC'y
Cuts at: 2905 3153 4175 5154 2905
Size: 248 1022 979 3222
Fragments arranged by size:
3222 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 289 321 353 1287 1319 1584 1616 1697 1717
Size: 32 32 934 32 265 32 81 20
Cuts at: 1717 1729 1749 3313 3343 3345 3375 289
Size: 12 20 1564 30 2 30 2385
Fragments arranged by size:
2385 1564 934 265 81 32 32 32
32 30 30 20 20 12 2
HincII GTy'rAC
Cuts at: 2510 2510
Size: 5471
HindIII A'AGCT_T
Cuts at: 1511 1511
Size: 5471
KasI G'GCGC_C
Cuts at: 2901 3149 2901
Size: 248 5223
KpnI G_GTAC'C
Cuts at: 2491 2491
Size: 5471
MfeI C'AATT_G
Cuts at: 581 1312 581
Size: 731 4740
MluI A'CGCG_T
Cuts at: 4904 4904
Size: 5471
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1208 4142 4326 1208
Size: 2934 184 2353
Fragments arranged by size:
2934 2353 184
MscI TGG'CCA
Cuts at: 2359 3232 2359
Size: 873 4598
MslI CAynn'nnrTG
Cuts at: 1686 1879 3460 3587 1686
Size: 193 1581 127 3570
Fragments arranged by size:
3570 1581 193 127
MspA1I CmG'CkG
Cuts at: 906 966 2582 2896 3256 4269 4514 906
Size: 60 1616 314 360 1013 245 1863
Fragments arranged by size:
1863 1616 1013 360 314 245 60
NaeI GCC'GGC
Cuts at: 3652 3652
Size: 5471
NarI GG'CG_CC
Cuts at: 2902 3150 2902
Size: 248 5223
NgoMIV G'CCGG_C
Cuts at: 3650 3650
Size: 5471
NsiI A_TGCA'T
Cuts at: 483 2025 483
Size: 1542 3929
NspI r_CATG'y
Cuts at: 1131 1824 2524 3024 3931 1131
Size: 693 700 500 907 2671
Fragments arranged by size:
2671 907 700 693 500
PciI A'CATG_T
Cuts at: 1820 3927 1820
Size: 2107 3364
PflMI CCAn_nnn'nTGG
Cuts at: 390 390
Size: 5471
PfoI T'CCnGG_A
Cuts at: 1557 1557
Size: 5471
PmeI GTTT'AAAC
Cuts at: 2470 2470
Size: 5471
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4610 4642 4610
Size: 32 5439
PsiI TTA'TAA
Cuts at: 1769 1769
Size: 5471
PstI C_TGCA'G
Cuts at: 1912 2518 3203 1912
Size: 606 685 4180
Fragments arranged by size:
4180 685 606
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5471
PvuII CAG'CTG
Cuts at: 906 966 2896 3256 906
Size: 60 1930 360 3121
Fragments arranged by size:
3121 1930 360 60
RsrII CG'GwC_CG
Cuts at: 3666 3666
Size: 5471
SacI G_AGCT'C
Cuts at: 2485 2485
Size: 5471
SalI G'TCGA_C
Cuts at: 2508 2508
Size: 5471
SapI GCTCTTCn'nnn_
Cuts at: 3494 3704 3494
Size: 210 5261
SbfI CC_TGCA'GG
Cuts at: 2518 2518
Size: 5471
ScaI AGT'ACT
Cuts at: 2317 4988 2317
Size: 2671 2800
SfcI C'TryA_G
Cuts at: 33 743 955 1908 2514 3199 4192 4383 33
Size: 710 212 953 606 685 993 191 1121
Fragments arranged by size:
1121 993 953 710 685 606 212 191
SfoI GGC'GCC
Cuts at: 2903 3151 2903
Size: 248 5223
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5471
SmaI CCC'GGG
Cuts at: 2493 2493
Size: 5471
SmlI C'TyrA_G
Cuts at: 454 2421 2626 3541 4033 4295 454
Size: 1967 205 915 492 262 1630
Fragments arranged by size:
1967 1630 915 492 262 205
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5471
SphI G_CATG'C
Cuts at: 2524 3024 2524
Size: 500 4971
SspI AAT'ATT
Cuts at: 2439 2439
Size: 5471
StuI AGG'CCT
Cuts at: 1009 1009
Size: 5471
StyI C'CwwG_G
Cuts at: 2384 2605 2959 2384
Size: 221 354 4896
Fragments arranged by size:
4896 354 221
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1634 2046 3054 3187 3646 3855 1634
Size: 412 1008 133 459 209 3250
Fragments arranged by size:
3250 1008 459 412 209 133
TatI w'GTAC_w
Cuts at: 2315 4986 2315
Size: 2671 2800
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1106 1138 4988 5020 1106
Size: 32 3850 32 1557
Fragments arranged by size:
3850 1557 32 32
Tth111I GACn'n_nGTC
Cuts at: 3268 3268
Size: 5471
XbaI T'CTAG_A
Cuts at: 2502 2502
Size: 5471
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1881 2966 1881
Size: 1085 4386
XmaI C'CCGG_G
Cuts at: 2491 2491
Size: 5471
XmnI GAAnn'nnTTC
Cuts at: 886 2306 886
Size: 1420 4051
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgZI BtsI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NsiI
NspI PciI PflMI PfoI PmeI PpiI PsiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AfeI AhdI AleI AloI ApaI AscI AvrII
BaeI BbvCI BcgI BglI BmgBI BmtI Bpu10I BseRI
BsiWI BsmBI BsrGI BssHII BstEII BstZ17I BtgI ClaI
FseI FspAI HpaI NcoI NdeI NheI NotI NruI
PacI PasI PmlI PpuMI PshAI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SwaI
XhoI ZraI