Restriction map for
pF1KA0018
(Circular) (Six-base) MAPSORT of: pF1KA0018.seq Check: 3574 from: 1 to: 4661
pF1KA0018 4661 bp
With 182 enzymes: SgfI *
June 20, 2008 10:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 327 327
Size: 4661
AccI GT'mk_AC
Cuts at: 1328 1699 1328
Size: 371 4290
Acc65I G'GTAC_C
Cuts at: 1677 1677
Size: 4661
AclI AA'CG_TT
Cuts at: 4501 4501
Size: 4661
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 187 1063 1225 2486 2918 3665 3933 187
Size: 876 162 1261 432 747 268 915
Fragments arranged by size:
1261 915 876 747 432 268 162
AfeI AGC'GCT
Cuts at: 199 294 199
Size: 95 4566
AflIII A'CryG_T
Cuts at: 414 2013 3117 4094 414
Size: 1599 1104 977 981
Fragments arranged by size:
1599 1104 981 977
AgeI A'CCGG_T
Cuts at: 3049 3049
Size: 4661
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 721 721
Size: 4661
AlwNI CAG_nnn'CTG
Cuts at: 1299 1463 1515 1966 3533 1299
Size: 164 52 451 1567 2427
Fragments arranged by size:
2427 1567 451 164 52
ApaLI G'TGCA_C
Cuts at: 378 1378 3431 378
Size: 1000 2053 1608
Fragments arranged by size:
2053 1608 1000
ApoI r'AATT_y
Cuts at: 1665 4014 4270 1665
Size: 2349 256 2056
Fragments arranged by size:
2349 2056 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4661
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4661
AvaI C'yCGr_G
Cuts at: 1618 1681 1618
Size: 63 4598
BamHI G'GATC_C
Cuts at: 1686 1686
Size: 4661
BanI G'GyrC_C
Cuts at: 386 1182 1272 1677 2091 2339 2374 386
Size: 796 90 405 414 248 35 2673
Fragments arranged by size:
2673 796 414 405 248 90 35
BanII G_rGCy'C
Cuts at: 116 512 977 1124 1406 1675 2705 116
Size: 396 465 147 282 269 1030 2072
Fragments arranged by size:
2072 1030 465 396 282 269 147
BbeI G_GCGC'C
Cuts at: 390 2095 2343 390
Size: 1705 248 2708
Fragments arranged by size:
2708 1705 248
BbsI GAAGACnn'nnnn_
Cuts at: 439 1051 439
Size: 612 4049
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 606 640 606
Size: 34 4627
BciVI GTATCCnnnnn_n'
Cuts at: 2559 3320 4661 2559
Size: 761 1341 2559
Fragments arranged by size:
2559 1341 761
BclI T'GATC_A
Cuts at: 452 608 2181 452
Size: 156 1573 2932
Fragments arranged by size:
2932 1573 156
BglI GCCn_nnn'nGGC
Cuts at: 1370 1370
Size: 4661
BglII A'GATC_T
Cuts at: 1193 1634 2176 4213 1193
Size: 441 542 2037 1641
Fragments arranged by size:
2037 1641 542 441
BlpI GC'TnA_GC
Cuts at: 286 1773 286
Size: 1487 3174
Bme1580I G_kGCm'C
Cuts at: 382 619 1277 1382 2286 2379 3435 382
Size: 237 658 105 904 93 1056 1608
Fragments arranged by size:
1608 1056 904 658 237 105 93
BmgBI CAC'GTC
Cuts at: 1326 1326
Size: 4661
BmrI ACTGGGnnnn_n'
Cuts at: 1873 1954 2289 4433 1873
Size: 81 335 2144 2101
Fragments arranged by size:
2144 2101 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3028 3028
Size: 4661
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 871 1084 1108 1498 196
Size: 675 213 24 390 3359
Fragments arranged by size:
3359 675 390 213 24
Bpu10I CC'TnA_GC
Cuts at: 578 578
Size: 4661
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1098 1837 2716 3208 3506 1098
Size: 739 879 492 298 2253
Fragments arranged by size:
2253 879 739 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1216 4365 1216
Size: 3149 1512
BsaAI yAC'GTr
Cuts at: 261 415 1254 2014 261
Size: 154 839 760 2908
Fragments arranged by size:
2908 839 760 154
BsaBI GATnn'nnATC
Cuts at: 6 796 2175 4212 6
Size: 790 1379 2037 455
Fragments arranged by size:
2037 1379 790 455
BsaHI Gr'CG_yC
Cuts at: 387 1610 2092 2340 3042 4538 387
Size: 1223 482 248 702 1496 510
Fragments arranged by size:
1496 1223 702 510 482 248
BsaWI w'CCGG_w
Cuts at: 1850 2371 3049 3323 3470 3881 1850
Size: 521 678 274 147 411 2630
Fragments arranged by size:
2630 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1558 1558
Size: 4661
BseYI C'CCAG_C
Cuts at: 200 768 884 976 1360 1596 2086 2844 3421
Size: 568 116 92 384 236 490 758 577
Cuts at: 3421 3917 200
Size: 496 944
Fragments arranged by size:
944 758 577 568 496 490 384 236
116 92
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 289 651 1279 1285 289
Size: 362 628 6 3665
Fragments arranged by size:
3665 628 362 6
BsiEI CG_ry'CG
Cuts at: 104 2203 2249 3457 104
Size: 2099 46 1208 1308
Fragments arranged by size:
2099 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 188 382 1124 1382 1406 1675 2453 2643 3435
Size: 194 742 258 24 269 778 190 792
Cuts at: 3435 188
Size: 1414
Fragments arranged by size:
1414 792 778 742 269 258 194 190
24
Bsp1286I G_dGCh'C
Cuts at: 116 188 382 512 619 977 1124 1277 1382
Size: 72 194 130 107 358 147 153 105
Cuts at: 1382 1406 1675 2286 2379 2453 2643 2705 3435
Size: 24 269 611 93 74 190 62 730
Cuts at: 3435 116
Size: 1342
Fragments arranged by size:
1342 730 611 358 269 194 190 153
147 130 107 105 93 74 72 62
24
BspEI T'CCGG_A
Cuts at: 3881 3881
Size: 4661
BspHI T'CATG_A
Cuts at: 449 956 3837 449
Size: 507 2881 1273
Fragments arranged by size:
2881 1273 507
BspMI ACCTGCnnnn'nnnn_
Cuts at: 327 1711 1925 2227 2608 327
Size: 1384 214 302 381 2380
Fragments arranged by size:
2380 1384 381 302 214
BsrBI CCG'CTC
Cuts at: 50 231 2953 3007 4491 4656 50
Size: 181 2722 54 1484 165 55
Fragments arranged by size:
2722 1484 181 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2573 2573
Size: 4661
BsrFI r'CCGG_y
Cuts at: 2659 2840 3049 2659
Size: 181 209 4271
Fragments arranged by size:
4271 209 181
BssHII G'CGCG_C
Cuts at: 137 264 137
Size: 127 4534
BssSI C'ACGA_G
Cuts at: 307 877 2932 3290 307
Size: 570 2055 358 1678
Fragments arranged by size:
2055 1678 570 358
BstAPI GCAn_nnn'nTGC
Cuts at: 318 1266 1299 2038 318
Size: 948 33 739 2941
Fragments arranged by size:
2941 948 739 33
BstBI TT'CG_AA
Cuts at: 3022 3022
Size: 4661
BstEII G'GTnAC_C
Cuts at: 516 516
Size: 4661
BstYI r'GATC_y
Cuts at: 1 791 1193 1634 1686 2153 2176 2511 2757
Size: 790 402 441 52 467 23 335 246
Cuts at: 2757 3769 3855 3867 4213 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 790 467 449 441 402 346 335
246 86 52 23 12
BtgI C'CryG_G
Cuts at: 107 521 1012 107
Size: 414 491 3756
Fragments arranged by size:
3756 491 414
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 723 2611 2792 2955 723
Size: 1888 181 163 2429
Fragments arranged by size:
2429 1888 181 163
BtsI GCAGTG_nn'
Cuts at: 668 1904 668
Size: 1236 3425
DraI TTT'AAA
Cuts at: 1660 1660
Size: 4661
DraIII CAC_nnn'GTG
Cuts at: 415 906 1254 1257 415
Size: 491 348 3 3819
Fragments arranged by size:
3819 491 348 3
DrdI GACnn_nn'nnGTC
Cuts at: 1324 2367 3225 1324
Size: 1043 858 2760
Fragments arranged by size:
2760 1043 858
EaeI y'GGCC_r
Cuts at: 128 782 2246 2420 2811 2838 4183 128
Size: 654 1464 174 391 27 1345 606
Fragments arranged by size:
1464 1345 654 606 391 174 27
EagI C'GGCC_G
Cuts at: 2246 2246
Size: 4661
EarI CTCTTCn'nnn_
Cuts at: 151 223 2684 2894 151
Size: 72 2461 210 1918
Fragments arranged by size:
2461 1918 210 72
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2869 3179 3325 4471 2869
Size: 310 146 1146 3059
Fragments arranged by size:
3059 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1122 1404 1673 1122
Size: 282 269 4110
Fragments arranged by size:
4110 282 269
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 187 196 871 1063 1084 1108 1225 1498 2486
Size: 9 675 192 21 24 117 273 988
Cuts at: 2486 2918 3665 3933 187
Size: 432 747 268 915
Fragments arranged by size:
988 915 747 675 432 273 268 192
117 24 21 9
EcoNI CCTnn'n_nnAGG
Cuts at: 931 1291 1467 931
Size: 360 176 4125
Fragments arranged by size:
4125 360 176
EcoO109I rG'GnC_Cy
Cuts at: 846 1059 1296 1460 1800 846
Size: 213 237 164 340 3707
Fragments arranged by size:
3707 340 237 213 164
EcoRI G'AATT_C
Cuts at: 1665 1665
Size: 4661
EcoRV GAT'ATC
Cuts at: 249 249
Size: 4661
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2680 2680
Size: 4661
FspI TGC'GCA
Cuts at: 1231 2442 1231
Size: 1211 3450
HaeII r_GCGC'y
Cuts at: 201 296 390 2095 2343 3365 4344 201
Size: 95 94 1705 248 1022 979 518
Fragments arranged by size:
1705 1022 979 518 248 95 94
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 619 651 1170 1202 2503 2533 2535 2565 619
Size: 32 519 32 1301 30 2 30 2715
Fragments arranged by size:
2715 1301 519 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1700 1700
Size: 4661
KasI G'GCGC_C
Cuts at: 386 2091 2339 386
Size: 1705 248 2708
Fragments arranged by size:
2708 1705 248
KpnI G_GTAC'C
Cuts at: 1681 1681
Size: 4661
MluI A'CGCG_T
Cuts at: 4094 4094
Size: 4661
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 681 3332 3516 681
Size: 2651 184 1826
Fragments arranged by size:
2651 1826 184
MscI TGG'CCA
Cuts at: 2422 2422
Size: 4661
MslI CAynn'nnrTG
Cuts at: 1270 2650 2777 1270
Size: 1380 127 3154
Fragments arranged by size:
3154 1380 127
MspA1I CmG'CkG
Cuts at: 787 888 1476 1772 2086 2446 3459 3704 787
Size: 101 588 296 314 360 1013 245 1744
Fragments arranged by size:
1744 1013 588 360 314 296 245 101
NaeI GCC'GGC
Cuts at: 2842 2842
Size: 4661
NarI GG'CG_CC
Cuts at: 387 2092 2340 387
Size: 1705 248 2708
Fragments arranged by size:
2708 1705 248
NcoI C'CATG_G
Cuts at: 107 521 107
Size: 414 4247
NdeI CA'TA_TG
Cuts at: 1425 1425
Size: 4661
NgoMIV G'CCGG_C
Cuts at: 2840 2840
Size: 4661
NspI r_CATG'y
Cuts at: 1030 1269 1714 2214 3121 1030
Size: 239 445 500 907 2570
Fragments arranged by size:
2570 907 500 445 239
PasI CC'CwG_GG
Cuts at: 1215 1215
Size: 4661
PciI A'CATG_T
Cuts at: 3117 3117
Size: 4661
PflMI CCAn_nnn'nTGG
Cuts at: 557 906 557
Size: 349 4312
PfoI T'CCnGG_A
Cuts at: 1124 1124
Size: 4661
PmeI GTTT'AAAC
Cuts at: 1660 1660
Size: 4661
PmlI CAC'GTG
Cuts at: 1254 1254
Size: 4661
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3800 3832 3800
Size: 32 4629
PpuMI rG'GwC_Cy
Cuts at: 1460 1460
Size: 4661
PshAI GACnn'nnGTC
Cuts at: 952 952
Size: 4661
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 637 669 637
Size: 32 4629
PstI C_TGCA'G
Cuts at: 1292 1708 2393 1292
Size: 416 685 3560
Fragments arranged by size:
3560 685 416
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4661
PvuII CAG'CTG
Cuts at: 1476 2086 2446 1476
Size: 610 360 3691
Fragments arranged by size:
3691 610 360
RsrII CG'GwC_CG
Cuts at: 2856 2856
Size: 4661
SacI G_AGCT'C
Cuts at: 1124 1406 1675 1124
Size: 282 269 4110
Fragments arranged by size:
4110 282 269
SalI G'TCGA_C
Cuts at: 1698 1698
Size: 4661
SapI GCTCTTCn'nnn_
Cuts at: 151 2684 2894 151
Size: 2533 210 1918
Fragments arranged by size:
2533 1918 210
SbfI CC_TGCA'GG
Cuts at: 1708 1708
Size: 4661
ScaI AGT'ACT
Cuts at: 4178 4178
Size: 4661
SfcI C'TryA_G
Cuts at: 33 1288 1704 2389 3382 3573 33
Size: 1255 416 685 993 191 1121
Fragments arranged by size:
1255 1121 993 685 416 191
SfoI GGC'GCC
Cuts at: 388 2093 2341 388
Size: 1705 248 2708
Fragments arranged by size:
2708 1705 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4661
SmaI CCC'GGG
Cuts at: 1683 1683
Size: 4661
SmlI C'TyrA_G
Cuts at: 1077 1816 2731 3223 3485 1077
Size: 739 915 492 262 2253
Fragments arranged by size:
2253 915 739 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4661
SphI G_CATG'C
Cuts at: 1714 2214 1714
Size: 500 4161
StuI AGG'CCT
Cuts at: 1373 1373
Size: 4661
StyI C'CwwG_G
Cuts at: 107 512 521 1795 2149 107
Size: 405 9 1274 354 2619
Fragments arranged by size:
2619 1274 405 354 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 147 192 261 889 2244 2377 2836 3045 147
Size: 45 69 628 1355 133 459 209 1763
Fragments arranged by size:
1763 1355 628 459 209 133 69 45
TatI w'GTAC_w
Cuts at: 425 1067 4176 425
Size: 642 3109 910
Fragments arranged by size:
3109 910 642
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4178 4210 4178
Size: 32 4629
Tth111I GACn'n_nGTC
Cuts at: 2458 2458
Size: 4661
XbaI T'CTAG_A
Cuts at: 1692 1692
Size: 4661
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2156 2156
Size: 4661
XmaI C'CCGG_G
Cuts at: 1681 1681
Size: 4661
XmnI GAAnn'nnTTC
Cuts at: 901 901
Size: 4661
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsrI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AleI AloI ApaI AscI AvrII
BaeI BbvCI BfrBI BmtI BsaXI BsiWI BsmI BsmBI
BsrGI BstXI BstZ17I Bsu36I ClaI CspCI FseI FspAI
HindIII HpaI MfeI NheI NotI NruI NsiI PacI
PsiI PspOMI PspXI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI ZraI