Restriction map for
pF1KA0034
(Circular) (Six-base) MAPSORT of: pF1KA0034.seq Check: 3038 from: 1 to: 8138
pF1KA0034 8138 bp
With 182 enzymes: SgfI *
March 2, 2010 14:24 ..
AccI GT'mk_AC
Cuts at: 2538 5176 2538
Size: 2638 5500
Acc65I G'GTAC_C
Cuts at: 976 5154 976
Size: 4178 3960
AclI AA'CG_TT
Cuts at: 748 3408 7978 748
Size: 2660 4570 908
Fragments arranged by size:
4570 2660 908
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 171 355 1208 1336 1411 1508 1837 1856 2603
Size: 184 853 128 75 97 329 19 747
Cuts at: 2603 3083 3329 4778 4937 5963 6395 6748 7410
Size: 480 246 1449 159 1026 432 353 662
Cuts at: 7410 171
Size: 899
Fragments arranged by size:
1449 1026 899 853 747 662 480 432
353 329 246 184 159 128 97 75
19
AflII C'TTAA_G
Cuts at: 2395 2395
Size: 8138
AflIII A'CryG_T
Cuts at: 3220 3222 5490 7290 7571 3220
Size: 2 2268 1800 281 3787
Fragments arranged by size:
3787 2268 1800 281 2
AleI CACnn'nnGTG
Cuts at: 3809 4406 3809
Size: 597 7541
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1811 3001 1811
Size: 1190 6948
AlwNI CAG_nnn'CTG
Cuts at: 120 159 2142 2199 3079 4545 5443 6881 120
Size: 39 1983 57 880 1466 898 1438 1377
Fragments arranged by size:
1983 1466 1438 1377 898 880 57 39
ApaLI G'TGCA_C
Cuts at: 2287 6976 2287
Size: 4689 3449
ApoI r'AATT_y
Cuts at: 214 1207 1277 2388 2702 2916 3328 3670 3901
Size: 993 70 1111 314 214 412 342 231
Cuts at: 3901 4084 4345 5142 7491 7747 214
Size: 183 261 797 2349 256 605
Fragments arranged by size:
2349 1111 993 797 605 412 342 314
261 256 231 214 183 70
AseI AT'TA_AT
Cuts at: 20 2900 20
Size: 2880 5258
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8138
AvaI C'yCGr_G
Cuts at: 3599 5158 3599
Size: 1559 6579
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 147 180 1347 1380 147
Size: 33 1167 33 6905
Fragments arranged by size:
6905 1167 33 33
BamHI G'GATC_C
Cuts at: 2254 2961 5163 2254
Size: 707 2202 5229
Fragments arranged by size:
5229 2202 707
BanI G'GyrC_C
Cuts at: 557 976 5064 5154 5568 5816 5851 557
Size: 419 4088 90 414 248 35 2844
Fragments arranged by size:
4088 2844 419 414 248 90 35
BanII G_rGCy'C
Cuts at: 1941 4044 5152 6182 1941
Size: 2103 1108 1030 3897
Fragments arranged by size:
3897 2103 1108 1030
BbeI G_GCGC'C
Cuts at: 5572 5820 5572
Size: 248 7890
BbsI GAAGACnn'nnnn_
Cuts at: 303 849 2096 303
Size: 546 1247 6345
Fragments arranged by size:
6345 1247 546
BbvCI CC'TCA_GC
Cuts at: 2648 2648
Size: 8138
BciVI GTATCCnnnnn_n'
Cuts at: 1375 1627 6036 7092 8138 1375
Size: 252 4409 1056 1046 1375
Fragments arranged by size:
4409 1375 1056 1046 252
BclI T'GATC_A
Cuts at: 4004 5658 4004
Size: 1654 6484
BfrBI ATG'CAT
Cuts at: 1878 1878
Size: 8138
BglII A'GATC_T
Cuts at: 5653 7690 5653
Size: 2037 6101
BlpI GC'TnA_GC
Cuts at: 4595 5250 4595
Size: 655 7483
Bme1580I G_kGCm'C
Cuts at: 2291 5763 5856 6980 2291
Size: 3472 93 1124 3449
Fragments arranged by size:
3472 3449 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1504 2330 3444 3564 4036 5013 5036 5350 5431
Size: 826 1114 120 472 977 23 314 81
Cuts at: 5431 5766 7910 1504
Size: 335 2144 1732
Fragments arranged by size:
2144 1732 1114 977 826 472 335 314
120 81 23
BmtI G_CTAG'C
Cuts at: 338 707 338
Size: 369 7769
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6505 6505
Size: 8138
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 130 136 516 800 851 1274 1625 3151 3450
Size: 6 380 284 51 423 351 1526 299
Cuts at: 3450 4135 130
Size: 685 4133
Fragments arranged by size:
4133 1526 685 423 380 351 299 284
51 6
Bpu10I CC'TnA_GC
Cuts at: 2375 2648 2742 2375
Size: 273 94 7771
Fragments arranged by size:
7771 273 94
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 988 1871 1882 4054 5314 6193 6907 7205 988
Size: 883 11 2172 1260 879 714 298 1921
Fragments arranged by size:
2172 1921 1260 883 879 714 298 11
BsaI GGTCTCn'nnnn_
Cuts at: 2242 3003 7842 2242
Size: 761 4839 2538
Fragments arranged by size:
4839 2538 761
BsaAI yAC'GTr
Cuts at: 1168 3223 5491 1168
Size: 2055 2268 3815
Fragments arranged by size:
3815 2268 2055
BsaBI GATnn'nnATC
Cuts at: 6 2849 5652 7689 6
Size: 2843 2803 2037 455
Fragments arranged by size:
2843 2803 2037 455
BsaHI Gr'CG_yC
Cuts at: 5569 5817 6519 8015 5569
Size: 248 702 1496 5692
Fragments arranged by size:
5692 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3323 5327 5848 6526 6937 7084 7358 3323
Size: 2004 521 678 411 147 274 4103
Fragments arranged by size:
4103 2004 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1444 1474 1444
Size: 30 8108
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1334 2734 1334
Size: 1400 6738
BseYI C'CCAG_C
Cuts at: 171 358 1320 1325 5563 6321 6986 7394 171
Size: 187 962 5 4238 758 665 408 915
Fragments arranged by size:
4238 962 915 758 665 408 187 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 605 1010 2042 4430 605
Size: 405 1032 2388 4313
Fragments arranged by size:
4313 2388 1032 405
BsiEI CG_ry'CG
Cuts at: 104 5680 5726 6956 104
Size: 5576 46 1230 1286
Fragments arranged by size:
5576 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 2291 2474 4044 4112 5152 5930 6120 6980 2291
Size: 183 1570 68 1040 778 190 860 3449
Fragments arranged by size:
3449 1570 1040 860 778 190 183 68
BsmI GAATG_Cn'
Cuts at: 1798 1798
Size: 8138
Bsp1286I G_dGCh'C
Cuts at: 1941 2291 2474 4044 4112 5152 5763 5856 5930
Size: 350 183 1570 68 1040 611 93 74
Cuts at: 5930 6120 6182 6980 1941
Size: 190 62 798 3099
Fragments arranged by size:
3099 1570 1040 798 611 350 190 183
93 74 68 62
BspEI T'CCGG_A
Cuts at: 3323 3323
Size: 8138
BspHI T'CATG_A
Cuts at: 323 1032 2175 2706 3092 4913 6570 323
Size: 709 1143 531 386 1821 1657 1891
Fragments arranged by size:
1891 1821 1657 1143 709 531 386
BspMI ACCTGCnnnn'nnnn_
Cuts at: 988 3429 5188 5402 5704 6085 988
Size: 2441 1759 214 302 381 3041
Fragments arranged by size:
3041 2441 1759 381 302 214
BsrBI CCG'CTC
Cuts at: 50 6430 6484 7968 8133 50
Size: 6380 54 1484 165 55
Fragments arranged by size:
6380 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 902 2916 3297 3420 4724 6050 902
Size: 2014 381 123 1304 1326 2990
Fragments arranged by size:
2990 2014 1326 1304 381 123
BsrFI r'CCGG_y
Cuts at: 1180 6136 6317 1180
Size: 4956 181 3001
Fragments arranged by size:
4956 3001 181
BsrGI T'GTAC_A
Cuts at: 2517 3030 3758 3939 2517
Size: 513 728 181 6716
Fragments arranged by size:
6716 728 513 181
BssSI C'ACGA_G
Cuts at: 4406 6409 7117 4406
Size: 2003 708 5427
Fragments arranged by size:
5427 2003 708
BstAPI GCAn_nnn'nTGC
Cuts at: 5515 5515
Size: 8138
BstBI TT'CG_AA
Cuts at: 294 6499 294
Size: 6205 1933
BstEII G'GTnAC_C
Cuts at: 3712 3712
Size: 8138
BstXI CCAn_nnnn'nTGG
Cuts at: 2972 3709 2972
Size: 737 7401
BstYI r'GATC_y
Cuts at: 1 446 2254 2844 2961 5163 5630 5653 5988
Size: 445 1808 590 117 2202 467 23 335
Cuts at: 5988 6234 6540 6552 6638 7690 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
2202 1808 1052 590 467 449 445 335
306 246 117 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1546 1546
Size: 8138
BtgI C'CryG_G
Cuts at: 107 4390 107
Size: 4283 3855
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 6088 6269 6432 6088
Size: 181 163 7794
Fragments arranged by size:
7794 181 163
BtsI GCAGTG_nn'
Cuts at: 854 1795 2030 3197 4777 5381 854
Size: 941 235 1167 1580 604 3611
Fragments arranged by size:
3611 1580 1167 941 604 235
ClaI AT'CG_AT
Cuts at: 2445 2445
Size: 8138
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 3528 3563 3528
Size: 35 8103
DraI TTT'AAA
Cuts at: 2295 5137 2295
Size: 2842 5296
DraIII CAC_nnn'GTG
Cuts at: 1537 1537
Size: 8138
DrdI GACnn_nn'nnGTC
Cuts at: 204 5844 7188 204
Size: 5640 1344 1154
Fragments arranged by size:
5640 1344 1154
EaeI y'GGCC_r
Cuts at: 2877 5723 5897 6288 6315 7660 2877
Size: 2846 174 391 27 1345 3355
Fragments arranged by size:
3355 2846 1345 391 174 27
EagI C'GGCC_G
Cuts at: 5723 5723
Size: 8138
EarI CTCTTCn'nnn_
Cuts at: 374 1721 2833 2929 3992 4654 6161 6371 374
Size: 1347 1112 96 1063 662 1507 210 2141
Fragments arranged by size:
2141 1507 1347 1112 1063 662 210 96
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 6346 7088 7234 7948 6346
Size: 742 146 714 6536
Fragments arranged by size:
6536 742 714 146
EcoICRI GAG'CTC
Cuts at: 4042 5150 4042
Size: 1108 7030
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 130 136 171 355 516 800 851 1208 1274
Size: 6 35 184 161 284 51 357 66
Cuts at: 1274 1336 1411 1508 1625 1837 1856 2603 3083
Size: 62 75 97 117 212 19 747 480
Cuts at: 3083 3151 3329 3450 4135 4778 4937 5963 6395
Size: 68 178 121 685 643 159 1026 432
Cuts at: 6395 6748 7410 130
Size: 353 662 858
Fragments arranged by size:
1026 858 747 685 662 643 480 432
357 353 284 212 184 178 161 159
121 117 97 75 68 66 62 51
35 19 6
EcoNI CCTnn'n_nnAGG
Cuts at: 4708 4708
Size: 8138
EcoO109I rG'GnC_Cy
Cuts at: 1840 2277 3057 5038 5277 1840
Size: 437 780 1981 239 4701
Fragments arranged by size:
4701 1981 780 437 239
EcoRI G'AATT_C
Cuts at: 1277 2702 2916 3901 4345 5142 1277
Size: 1425 214 985 444 797 4273
Fragments arranged by size:
4273 1425 985 797 444 214
EcoRV GAT'ATC
Cuts at: 4287 4287
Size: 8138
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1750 3017 4192 4720 4941 6157 1750
Size: 1267 1175 528 221 1216 3731
Fragments arranged by size:
3731 1267 1216 1175 528 221
FspI TGC'GCA
Cuts at: 5919 5919
Size: 8138
HaeII r_GCGC'y
Cuts at: 320 5572 5820 7050 7821 320
Size: 5252 248 1230 771 637
Fragments arranged by size:
5252 1230 771 637 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 3496 3528 4233 4265 4570 4602 5980 6010 6012
Size: 32 705 32 305 32 1378 30 2
Cuts at: 6012 6042 3496
Size: 30 5592
Fragments arranged by size:
5592 1378 705 305 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2064 2189 2265 2539 2574 3436 5177 2064
Size: 125 76 274 35 862 1741 5025
Fragments arranged by size:
5025 1741 862 274 125 76 35
HpaI GTT'AAC
Cuts at: 2265 2265
Size: 8138
KasI G'GCGC_C
Cuts at: 5568 5816 5568
Size: 248 7890
KpnI G_GTAC'C
Cuts at: 980 5158 980
Size: 4178 3960
MluI A'CGCG_T
Cuts at: 7571 7571
Size: 8138
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1611 2802 3726 3995 4276 4376 6897 7081 1611
Size: 1191 924 269 281 100 2521 184 2668
Fragments arranged by size:
2668 2521 1191 924 281 269 184 100
MscI TGG'CCA
Cuts at: 2879 5899 2879
Size: 3020 5118
MslI CAynn'nnrTG
Cuts at: 268 388 959 988 3809 3947 4051 4406 6127
Size: 120 571 29 2821 138 104 355 1721
Cuts at: 6127 6254 268
Size: 127 2152
Fragments arranged by size:
2821 2152 1721 571 355 138 127 120
104 29
MspA1I CmG'CkG
Cuts at: 1045 3826 5249 5563 5923 6707 6952 1045
Size: 2781 1423 314 360 784 245 2231
Fragments arranged by size:
2781 2231 1423 784 360 314 245
NaeI GCC'GGC
Cuts at: 6319 6319
Size: 8138
NarI GG'CG_CC
Cuts at: 5569 5817 5569
Size: 248 7890
NcoI C'CATG_G
Cuts at: 107 107
Size: 8138
NdeI CA'TA_TG
Cuts at: 3393 3393
Size: 8138
NgoMIV G'CCGG_C
Cuts at: 6317 6317
Size: 8138
NheI G'CTAG_C
Cuts at: 334 703 334
Size: 369 7769
NsiI A_TGCA'T
Cuts at: 1880 1880
Size: 8138
NspI r_CATG'y
Cuts at: 700 1882 3946 5191 5691 7294 700
Size: 1182 2064 1245 500 1603 1544
Fragments arranged by size:
2064 1603 1544 1245 1182 500
PasI CC'CwG_GG
Cuts at: 1227 1227
Size: 8138
PciI A'CATG_T
Cuts at: 7290 7290
Size: 8138
PflMI CCAn_nnn'nTGG
Cuts at: 159 2466 5098 5113 159
Size: 2307 2632 15 3184
Fragments arranged by size:
3184 2632 2307 15
PmeI GTTT'AAAC
Cuts at: 5137 5137
Size: 8138
PmlI CAC'GTG
Cuts at: 3223 3223
Size: 8138
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6584 6616 6584
Size: 32 8106
PpuMI rG'GwC_Cy
Cuts at: 1840 2277 3057 5038 1840
Size: 437 780 1981 4940
Fragments arranged by size:
4940 1981 780 437
PstI C_TGCA'G
Cuts at: 1979 2147 3102 4164 4772 5185 5870 1979
Size: 168 955 1062 608 413 685 4247
Fragments arranged by size:
4247 1062 955 685 608 413 168
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8138
PvuII CAG'CTG
Cuts at: 1045 3826 5563 5923 1045
Size: 2781 1737 360 3260
Fragments arranged by size:
3260 2781 1737 360
RsrII CG'GwC_CG
Cuts at: 6333 6333
Size: 8138
SacI G_AGCT'C
Cuts at: 4044 5152 4044
Size: 1108 7030
SalI G'TCGA_C
Cuts at: 2537 5175 2537
Size: 2638 5500
SapI GCTCTTCn'nnn_
Cuts at: 1721 3992 6161 6371 1721
Size: 2271 2169 210 3488
Fragments arranged by size:
3488 2271 2169 210
SbfI CC_TGCA'GG
Cuts at: 5185 5185
Size: 8138
ScaI AGT'ACT
Cuts at: 1357 3574 3855 4924 7655 1357
Size: 2217 281 1069 2731 1840
Fragments arranged by size:
2731 2217 1840 1069 281
SexAI A'CCwGG_T
Cuts at: 2945 3023 2945
Size: 78 8060
SfcI C'TryA_G
Cuts at: 33 662 818 1975 2143 2349 2546 3098 4160
Size: 629 156 1157 168 206 197 552 1062
Cuts at: 4160 4320 4768 4985 5181 5866 6834 7025 33
Size: 160 448 217 196 685 968 191 1146
Fragments arranged by size:
1157 1146 1062 968 685 629 552 448
217 206 197 196 191 168 160 156
SfoI GGC'GCC
Cuts at: 5570 5818 5570
Size: 248 7890
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8138
SmaI CCC'GGG
Cuts at: 5160 5160
Size: 8138
SmlI C'TyrA_G
Cuts at: 967 1861 1886 2395 3599 4033 5293 6208 6922
Size: 894 25 509 1204 434 1260 915 714
Cuts at: 6922 7184 967
Size: 262 1921
Fragments arranged by size:
1921 1260 1204 915 894 714 509 434
262 25
SnaBI TAC'GTA
Cuts at: 1168 1168
Size: 8138
SpeI A'CTAG_T
Cuts at: 67 3544 67
Size: 3477 4661
SphI G_CATG'C
Cuts at: 1882 5191 5691 1882
Size: 3309 500 4329
Fragments arranged by size:
4329 3309 500
SspI AAT'ATT
Cuts at: 4140 4140
Size: 8138
StuI AGG'CCT
Cuts at: 1419 1419
Size: 8138
StyI C'CwwG_G
Cuts at: 107 2686 4145 4295 5272 5626 107
Size: 2579 1459 150 977 354 2619
Fragments arranged by size:
2619 2579 1459 977 354 150
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1486 2050 3799 5721 5854 6313 6522 1486
Size: 564 1749 1922 133 459 209 3102
Fragments arranged by size:
3102 1922 1749 564 459 209 133
TatI w'GTAC_w
Cuts at: 1355 1422 1792 2517 3030 3572 3758 3853 3939
Size: 67 370 725 513 542 186 95 86
Cuts at: 3939 4922 7653 1355
Size: 983 2731 1840
Fragments arranged by size:
2731 1840 983 725 542 513 370 186
95 86 67
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4662 4694 7655 7687 4662
Size: 32 2961 32 5113
Fragments arranged by size:
5113 2961 32 32
Tth111I GACn'n_nGTC
Cuts at: 2455 5935 2455
Size: 3480 4658
XbaI T'CTAG_A
Cuts at: 4129 5169 4129
Size: 1040 7098
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4259 5068 5633 4259
Size: 809 565 6764
Fragments arranged by size:
6764 809 565
XhoI C'TCGA_G
Cuts at: 3599 3599
Size: 8138
XmaI C'CCGG_G
Cuts at: 5158 5158
Size: 8138
XmnI GAAnn'nnTTC
Cuts at: 3527 3901 3527
Size: 374 7764
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI
BglII BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HpaI KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NsiI NspI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AgeI AhdI AloI ApaI AscI
AvrII BcgI BglI BmgBI BsiWI BsmBI BssHII BstZ17I
FseI FspAI HindIII MfeI NotI NruI PacI PfoI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SfiI
SgrAI SrfI SwaI ZraI