Restriction map for
pF1KA0038
(Circular) (Six-base) MAPSORT of: pF1KA0038.seq Check: 2198 from: 1 to: 3797
pF1KA0038 3797 bp
With 182 enzymes: SgfI *
June 20, 2008 10:18 ..
AccI GT'mk_AC
Cuts at: 835 835
Size: 3797
Acc65I G'GTAC_C
Cuts at: 813 813
Size: 3797
AclI AA'CG_TT
Cuts at: 3637 3637
Size: 3797
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 504 582 1622 2054 2801 3069 504
Size: 78 1040 432 747 268 1232
Fragments arranged by size:
1232 1040 747 432 268 78
AflII C'TTAA_G
Cuts at: 391 391
Size: 3797
AflIII A'CryG_T
Cuts at: 1149 2253 3230 1149
Size: 1104 977 1716
Fragments arranged by size:
1716 1104 977
AgeI A'CCGG_T
Cuts at: 2185 2185
Size: 3797
AlwNI CAG_nnn'CTG
Cuts at: 1102 2669 1102
Size: 1567 2230
ApaLI G'TGCA_C
Cuts at: 416 2567 416
Size: 2151 1646
ApoI r'AATT_y
Cuts at: 346 370 801 3150 3406 346
Size: 24 431 2349 256 737
Fragments arranged by size:
2349 737 431 256 24
AseI AT'TA_AT
Cuts at: 20 20
Size: 3797
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3797
AvaI C'yCGr_G
Cuts at: 817 817
Size: 3797
BamHI G'GATC_C
Cuts at: 622 822 622
Size: 200 3597
BanI G'GyrC_C
Cuts at: 751 813 1227 1475 1510 751
Size: 62 414 248 35 3038
Fragments arranged by size:
3038 414 248 62 35
BanII G_rGCy'C
Cuts at: 227 811 1841 227
Size: 584 1030 2183
Fragments arranged by size:
2183 1030 584
BbeI G_GCGC'C
Cuts at: 1231 1479 1231
Size: 248 3549
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 265 299 265
Size: 34 3763
BciVI GTATCCnnnnn_n'
Cuts at: 1695 2456 3797 1695
Size: 761 1341 1695
Fragments arranged by size:
1695 1341 761
BclI T'GATC_A
Cuts at: 1317 1317
Size: 3797
BglII A'GATC_T
Cuts at: 1312 3349 1312
Size: 2037 1760
BlpI GC'TnA_GC
Cuts at: 909 909
Size: 3797
Bme1580I G_kGCm'C
Cuts at: 420 1422 1515 2571 420
Size: 1002 93 1056 1646
Fragments arranged by size:
1646 1056 1002 93
BmrI ACTGGGnnnn_n'
Cuts at: 1009 1090 1425 3569 1009
Size: 81 335 2144 1237
Fragments arranged by size:
2144 1237 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2164 2164
Size: 3797
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 664 664
Size: 3797
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 973 1852 2344 2642 973
Size: 879 492 298 2128
Fragments arranged by size:
2128 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 663 3501 663
Size: 2838 959
BsaAI yAC'GTr
Cuts at: 243 1150 243
Size: 907 2890
BsaBI GATnn'nnATC
Cuts at: 6 1311 3348 6
Size: 1305 2037 455
Fragments arranged by size:
2037 1305 455
BsaHI Gr'CG_yC
Cuts at: 706 1228 1476 2178 3674 706
Size: 522 248 702 1496 829
Fragments arranged by size:
1496 829 702 522 248
BsaWI w'CCGG_w
Cuts at: 986 1507 2185 2459 2606 3017 986
Size: 521 678 274 147 411 1766
Fragments arranged by size:
1766 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 662 692 662
Size: 30 3767
BseYI C'CCAG_C
Cuts at: 1222 1980 2557 3053 1222
Size: 758 577 496 1966
Fragments arranged by size:
1966 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 135 432 567 1339 1385 2593 104
Size: 31 297 135 772 46 1208 1308
Fragments arranged by size:
1308 1208 772 297 135 46 31
BsiHKAI G_wGCw'C
Cuts at: 420 666 811 1589 1779 2571 420
Size: 246 145 778 190 792 1646
Fragments arranged by size:
1646 792 778 246 190 145
Bsp1286I G_dGCh'C
Cuts at: 227 420 666 811 1422 1515 1589 1779 1841
Size: 193 246 145 611 93 74 190 62
Cuts at: 1841 2571 227
Size: 730 1453
Fragments arranged by size:
1453 730 611 246 193 190 145 93
74 62
BspEI T'CCGG_A
Cuts at: 3017 3017
Size: 3797
BspHI T'CATG_A
Cuts at: 2973 2973
Size: 3797
BspMI ACCTGCnnnn'nnnn_
Cuts at: 847 1061 1363 1744 847
Size: 214 302 381 2900
Fragments arranged by size:
2900 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2089 2143 3627 3792 50
Size: 2039 54 1484 165 55
Fragments arranged by size:
2039 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 449 1709 449
Size: 1260 2537
BsrFI r'CCGG_y
Cuts at: 566 1795 1976 2185 566
Size: 1229 181 209 2178
Fragments arranged by size:
2178 1229 209 181
BssSI C'ACGA_G
Cuts at: 2068 2426 2068
Size: 358 3439
BstAPI GCAn_nnn'nTGC
Cuts at: 1174 1174
Size: 3797
BstBI TT'CG_AA
Cuts at: 2158 2158
Size: 3797
BstYI r'GATC_y
Cuts at: 1 297 622 822 1289 1312 1647 1893 2905
Size: 296 325 200 467 23 335 246 1012
Cuts at: 2905 2991 3003 3349 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 449 346 335 325 296 246
200 86 23 12
BtgI C'CryG_G
Cuts at: 107 171 189 584 618 107
Size: 64 18 395 34 3286
Fragments arranged by size:
3286 395 64 34 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1747 1928 2091 1747
Size: 181 163 3453
Fragments arranged by size:
3453 181 163
BtsI GCAGTG_nn'
Cuts at: 183 1040 183
Size: 857 2940
DraI TTT'AAA
Cuts at: 351 796 351
Size: 445 3352
DraIII CAC_nnn'GTG
Cuts at: 442 442
Size: 3797
DrdI GACnn_nn'nnGTC
Cuts at: 1503 2361 1503
Size: 858 2939
EaeI y'GGCC_r
Cuts at: 186 1382 1556 1947 1974 3319 186
Size: 1196 174 391 27 1345 664
Fragments arranged by size:
1345 1196 664 391 174 27
EagI C'GGCC_G
Cuts at: 1382 1382
Size: 3797
EarI CTCTTCn'nnn_
Cuts at: 647 1820 2030 647
Size: 1173 210 2414
Fragments arranged by size:
2414 1173 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 2005 2315 2461 3607 127
Size: 1878 310 146 1146 317
Fragments arranged by size:
1878 1146 317 310 146
EcoICRI GAG'CTC
Cuts at: 809 809
Size: 3797
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 504 582 664 1622 2054 2801 3069 504
Size: 78 82 958 432 747 268 1232
Fragments arranged by size:
1232 958 747 432 268 82 78
EcoO109I rG'GnC_Cy
Cuts at: 167 577 936 167
Size: 410 359 3028
Fragments arranged by size:
3028 410 359
EcoRI G'AATT_C
Cuts at: 370 801 370
Size: 431 3366
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 417 1816 417
Size: 1399 2398
FspI TGC'GCA
Cuts at: 1578 1578
Size: 3797
HaeII r_GCGC'y
Cuts at: 1231 1479 2501 3480 1231
Size: 248 1022 979 1548
Fragments arranged by size:
1548 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 662 694 755 787 1639 1669 1671 1701 662
Size: 32 61 32 852 30 2 30 2758
Fragments arranged by size:
2758 852 61 32 32 30 30 2
HincII GTy'rAC
Cuts at: 836 836
Size: 3797
KasI G'GCGC_C
Cuts at: 1227 1475 1227
Size: 248 3549
KpnI G_GTAC'C
Cuts at: 817 817
Size: 3797
MluI A'CGCG_T
Cuts at: 3230 3230
Size: 3797
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 650 2468 2652 650
Size: 1818 184 1795
Fragments arranged by size:
1818 1795 184
MscI TGG'CCA
Cuts at: 188 1558 188
Size: 1370 2427
MslI CAynn'nnrTG
Cuts at: 1786 1913 1786
Size: 127 3670
MspA1I CmG'CkG
Cuts at: 173 908 1222 1582 2595 2840 173
Size: 735 314 360 1013 245 1130
Fragments arranged by size:
1130 1013 735 360 314 245
NaeI GCC'GGC
Cuts at: 1978 1978
Size: 3797
NarI GG'CG_CC
Cuts at: 1228 1476 1228
Size: 248 3549
NcoI C'CATG_G
Cuts at: 107 189 584 107
Size: 82 395 3320
Fragments arranged by size:
3320 395 82
NgoMIV G'CCGG_C
Cuts at: 1976 1976
Size: 3797
NruI TCG'CGA
Cuts at: 703 703
Size: 3797
NspI r_CATG'y
Cuts at: 850 1350 2257 850
Size: 500 907 2390
Fragments arranged by size:
2390 907 500
PciI A'CATG_T
Cuts at: 2253 2253
Size: 3797
PmeI GTTT'AAAC
Cuts at: 796 796
Size: 3797
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2936 2968 2936
Size: 32 3765
PpuMI rG'GwC_Cy
Cuts at: 167 167
Size: 3797
PstI C_TGCA'G
Cuts at: 844 1529 844
Size: 685 3112
PvuI CG_AT'CG
Cuts at: 104 135 432 104
Size: 31 297 3469
Fragments arranged by size:
3469 297 31
PvuII CAG'CTG
Cuts at: 1222 1582 1222
Size: 360 3437
RsrII CG'GwC_CG
Cuts at: 1992 1992
Size: 3797
SacI G_AGCT'C
Cuts at: 811 811
Size: 3797
SacII CC_GC'GG
Cuts at: 174 174
Size: 3797
SalI G'TCGA_C
Cuts at: 834 834
Size: 3797
SanDI GG'GwC_CC
Cuts at: 167 167
Size: 3797
SapI GCTCTTCn'nnn_
Cuts at: 1820 2030 1820
Size: 210 3587
SbfI CC_TGCA'GG
Cuts at: 844 844
Size: 3797
ScaI AGT'ACT
Cuts at: 3314 3314
Size: 3797
SfcI C'TryA_G
Cuts at: 33 141 840 1525 2518 2709 33
Size: 108 699 685 993 191 1121
Fragments arranged by size:
1121 993 699 685 191 108
SfoI GGC'GCC
Cuts at: 1229 1477 1229
Size: 248 3549
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3797
SmaI CCC'GGG
Cuts at: 819 819
Size: 3797
SmlI C'TyrA_G
Cuts at: 391 952 1867 2359 2621 391
Size: 561 915 492 262 1567
Fragments arranged by size:
1567 915 561 492 262
SnaBI TAC'GTA
Cuts at: 243 243
Size: 3797
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3797
SphI G_CATG'C
Cuts at: 850 1350 850
Size: 500 3297
StuI AGG'CCT
Cuts at: 759 759
Size: 3797
StyI C'CwwG_G
Cuts at: 107 189 584 931 1285 107
Size: 82 395 347 354 2619
Fragments arranged by size:
2619 395 354 347 82
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 422 1380 1513 1972 2181 422
Size: 958 133 459 209 2038
Fragments arranged by size:
2038 958 459 209 133
TatI w'GTAC_w
Cuts at: 3312 3312
Size: 3797
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3314 3346 3314
Size: 32 3765
Tth111I GACn'n_nGTC
Cuts at: 1594 1594
Size: 3797
XbaI T'CTAG_A
Cuts at: 828 828
Size: 3797
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1292 1292
Size: 3797
XmaI C'CCGG_G
Cuts at: 817 817
Size: 3797
XmnI GAAnn'nnTTC
Cuts at: 773 773
Size: 3797
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NruI NspI PciI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BfrBI BglI BmgBI
BmtI Bpu10I BseRI BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NsiI PacI PasI PflMI PfoI PmlI PshAI
PsiI PspOMI PspXI PsrI SexAI SfiI SgrAI SrfI
SspI SwaI XhoI ZraI