Restriction map for
pF1KA0040
(Circular) (Six-base) MAPSORT of: pF1KA0040.seq Check: 4336 from: 1 to: 3572
pF1KA0040 3572 bp
With 182 enzymes: SgfI *
October 7, 2009 14:13 ..
AccI GT'mk_AC
Cuts at: 610 610
Size: 3572
Acc65I G'GTAC_C
Cuts at: 588 588
Size: 3572
AclI AA'CG_TT
Cuts at: 3412 3412
Size: 3572
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1397 1829 2576 2844 1397
Size: 432 747 268 2125
Fragments arranged by size:
2125 747 432 268
AflIII A'CryG_T
Cuts at: 924 2028 3005 924
Size: 1104 977 1491
Fragments arranged by size:
1491 1104 977
AgeI A'CCGG_T
Cuts at: 1960 1960
Size: 3572
AhdI GACnn_n'nnGTC
Cuts at: 167 167
Size: 3572
AlwNI CAG_nnn'CTG
Cuts at: 877 2444 877
Size: 1567 2005
ApaLI G'TGCA_C
Cuts at: 355 2342 355
Size: 1987 1585
ApoI r'AATT_y
Cuts at: 384 576 2925 3181 384
Size: 192 2349 256 775
Fragments arranged by size:
2349 775 256 192
AseI AT'TA_AT
Cuts at: 20 20
Size: 3572
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3572
AvaI C'yCGr_G
Cuts at: 592 592
Size: 3572
BamHI G'GATC_C
Cuts at: 597 597
Size: 3572
BanI G'GyrC_C
Cuts at: 588 1002 1250 1285 588
Size: 414 248 35 2875
Fragments arranged by size:
2875 414 248 35
BanII G_rGCy'C
Cuts at: 586 1616 586
Size: 1030 2542
BbeI G_GCGC'C
Cuts at: 1006 1254 1006
Size: 248 3324
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3538
BciVI GTATCCnnnnn_n'
Cuts at: 207 1470 2231 3572 207
Size: 1263 761 1341 207
Fragments arranged by size:
1341 1263 761 207
BclI T'GATC_A
Cuts at: 1092 1092
Size: 3572
BglII A'GATC_T
Cuts at: 1087 3124 1087
Size: 2037 1535
BlpI GC'TnA_GC
Cuts at: 684 684
Size: 3572
Bme1580I G_kGCm'C
Cuts at: 359 1197 1290 2346 359
Size: 838 93 1056 1585
Fragments arranged by size:
1585 1056 838 93
BmrI ACTGGGnnnn_n'
Cuts at: 167 784 865 1200 3344 167
Size: 617 81 335 2144 395
Fragments arranged by size:
2144 617 395 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1939 1939
Size: 3572
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 748 1627 2119 2417 748
Size: 879 492 298 1903
Fragments arranged by size:
1903 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3276 3276
Size: 3572
BsaAI yAC'GTr
Cuts at: 199 366 402 925 199
Size: 167 36 523 2846
Fragments arranged by size:
2846 523 167 36
BsaBI GATnn'nnATC
Cuts at: 6 1086 3123 6
Size: 1080 2037 455
Fragments arranged by size:
2037 1080 455
BsaHI Gr'CG_yC
Cuts at: 1003 1251 1953 3449 1003
Size: 248 702 1496 1126
Fragments arranged by size:
1496 1126 702 248
BsaWI w'CCGG_w
Cuts at: 761 1282 1960 2234 2381 2792 761
Size: 521 678 274 147 411 1541
Fragments arranged by size:
1541 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 997 1755 2332 2828 997
Size: 758 577 496 1741
Fragments arranged by size:
1741 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 472 472
Size: 3572
BsiEI CG_ry'CG
Cuts at: 104 1114 1160 2368 104
Size: 1010 46 1208 1308
Fragments arranged by size:
1308 1208 1010 46
BsiHKAI G_wGCw'C
Cuts at: 359 586 1364 1554 2346 359
Size: 227 778 190 792 1585
Fragments arranged by size:
1585 792 778 227 190
Bsp1286I G_dGCh'C
Cuts at: 359 586 1197 1290 1364 1554 1616 2346 359
Size: 227 611 93 74 190 62 730 1585
Fragments arranged by size:
1585 730 611 227 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2792 2792
Size: 3572
BspHI T'CATG_A
Cuts at: 2748 2748
Size: 3572
BspMI ACCTGCnnnn'nnnn_
Cuts at: 386 622 836 1138 1519 386
Size: 236 214 302 381 2439
Fragments arranged by size:
2439 381 302 236 214
BsrBI CCG'CTC
Cuts at: 50 1864 1918 3402 3567 50
Size: 1814 54 1484 165 55
Fragments arranged by size:
1814 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 251 1484 251
Size: 1233 2339
BsrFI r'CCGG_y
Cuts at: 1570 1751 1960 1570
Size: 181 209 3182
Fragments arranged by size:
3182 209 181
BssSI C'ACGA_G
Cuts at: 1843 2201 1843
Size: 358 3214
BstAPI GCAn_nnn'nTGC
Cuts at: 949 949
Size: 3572
BstBI TT'CG_AA
Cuts at: 1933 1933
Size: 3572
BstYI r'GATC_y
Cuts at: 1 597 1064 1087 1422 1668 2680 2766 2778
Size: 596 467 23 335 246 1012 86 12
Cuts at: 2778 3124 1
Size: 346 449
Fragments arranged by size:
1012 596 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1522 1703 1866 1522
Size: 181 163 3228
Fragments arranged by size:
3228 181 163
BtsI GCAGTG_nn'
Cuts at: 815 815
Size: 3572
DraI TTT'AAA
Cuts at: 571 571
Size: 3572
DrdI GACnn_nn'nnGTC
Cuts at: 1278 2136 1278
Size: 858 2714
EaeI y'GGCC_r
Cuts at: 1157 1331 1722 1749 3094 1157
Size: 174 391 27 1345 1635
Fragments arranged by size:
1635 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1157 1157
Size: 3572
EarI CTCTTCn'nnn_
Cuts at: 1595 1805 1595
Size: 210 3362
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1780 2090 2236 3382 1780
Size: 310 146 1146 1970
Fragments arranged by size:
1970 1146 310 146
EcoICRI GAG'CTC
Cuts at: 584 584
Size: 3572
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1397 1829 2576 2844 1397
Size: 432 747 268 2125
Fragments arranged by size:
2125 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 544 711 544
Size: 167 3405
EcoRI G'AATT_C
Cuts at: 576 576
Size: 3572
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1591 1591
Size: 3572
FspI TGC'GCA
Cuts at: 1353 1353
Size: 3572
HaeII r_GCGC'y
Cuts at: 1006 1254 2276 3255 1006
Size: 248 1022 979 1323
Fragments arranged by size:
1323 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 153 185 1414 1444 1446 1476 153
Size: 32 1229 30 2 30 2249
Fragments arranged by size:
2249 1229 32 30 30 2
HincII GTy'rAC
Cuts at: 611 611
Size: 3572
KasI G'GCGC_C
Cuts at: 1002 1250 1002
Size: 248 3324
KpnI G_GTAC'C
Cuts at: 592 592
Size: 3572
MluI A'CGCG_T
Cuts at: 3005 3005
Size: 3572
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2243 2427 2243
Size: 184 3388
MscI TGG'CCA
Cuts at: 1333 1333
Size: 3572
MslI CAynn'nnrTG
Cuts at: 481 1561 1688 481
Size: 1080 127 2365
Fragments arranged by size:
2365 1080 127
MspA1I CmG'CkG
Cuts at: 683 997 1357 2370 2615 683
Size: 314 360 1013 245 1640
Fragments arranged by size:
1640 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1753 1753
Size: 3572
NarI GG'CG_CC
Cuts at: 1003 1251 1003
Size: 248 3324
NgoMIV G'CCGG_C
Cuts at: 1751 1751
Size: 3572
NspI r_CATG'y
Cuts at: 273 304 363 625 1125 2032 273
Size: 31 59 262 500 907 1813
Fragments arranged by size:
1813 907 500 262 59 31
PasI CC'CwG_GG
Cuts at: 189 189
Size: 3572
PciI A'CATG_T
Cuts at: 2028 2028
Size: 3572
PmeI GTTT'AAAC
Cuts at: 571 571
Size: 3572
PmlI CAC'GTG
Cuts at: 199 199
Size: 3572
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2711 2743 2711
Size: 32 3540
PstI C_TGCA'G
Cuts at: 261 619 1304 261
Size: 358 685 2529
Fragments arranged by size:
2529 685 358
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3572
PvuII CAG'CTG
Cuts at: 997 1357 997
Size: 360 3212
RsrII CG'GwC_CG
Cuts at: 1767 1767
Size: 3572
SacI G_AGCT'C
Cuts at: 586 586
Size: 3572
SalI G'TCGA_C
Cuts at: 609 609
Size: 3572
SapI GCTCTTCn'nnn_
Cuts at: 1595 1805 1595
Size: 210 3362
SbfI CC_TGCA'GG
Cuts at: 619 619
Size: 3572
ScaI AGT'ACT
Cuts at: 3089 3089
Size: 3572
SfcI C'TryA_G
Cuts at: 33 257 470 615 1300 2293 2484 33
Size: 224 213 145 685 993 191 1121
Fragments arranged by size:
1121 993 685 224 213 191 145
SfoI GGC'GCC
Cuts at: 1004 1252 1004
Size: 248 3324
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3572
SmaI CCC'GGG
Cuts at: 594 594
Size: 3572
SmlI C'TyrA_G
Cuts at: 727 1642 2134 2396 727
Size: 915 492 262 1903
Fragments arranged by size:
1903 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3572
SphI G_CATG'C
Cuts at: 273 625 1125 273
Size: 352 500 2720
Fragments arranged by size:
2720 500 352
StyI C'CwwG_G
Cuts at: 145 706 1060 145
Size: 561 354 2657
Fragments arranged by size:
2657 561 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1155 1288 1747 1956 1155
Size: 133 459 209 2771
Fragments arranged by size:
2771 459 209 133
TatI w'GTAC_w
Cuts at: 443 460 3087 443
Size: 17 2627 928
Fragments arranged by size:
2627 928 17
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3089 3121 3089
Size: 32 3540
Tth111I GACn'n_nGTC
Cuts at: 1369 1369
Size: 3572
XbaI T'CTAG_A
Cuts at: 603 603
Size: 3572
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 196 1067 196
Size: 871 2701
XmaI C'CCGG_G
Cuts at: 592 592
Size: 3572
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PasI PciI PmeI PmlI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BfrBI BglI BmgBI
BmtI BpmI Bpu10I BsaXI BseRI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I BtgI ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PflMI PfoI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI