Restriction map for
pF1KA0041
(Circular) (Six-base) MAPSORT of: pF1KA0041.seq Check: 6361 from: 1 to: 5447
pF1KA0041 5447 bp
With 182 enzymes: SgfI *
June 20, 2008 10:18 ..
AccI GT'mk_AC
Cuts at: 2485 2485
Size: 5447
Acc65I G'GTAC_C
Cuts at: 2463 2463
Size: 5447
AclI AA'CG_TT
Cuts at: 5287 5287
Size: 5447
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 141 160 418 616 1297 1730 1892 1915 3272
Size: 19 258 198 681 433 162 23 1357
Cuts at: 3272 3704 4451 4719 141
Size: 432 747 268 869
Fragments arranged by size:
1357 869 747 681 433 432 268 258
198 162 23 19
AflIII A'CryG_T
Cuts at: 2074 2799 3903 4880 2074
Size: 725 1104 977 2641
Fragments arranged by size:
2641 1104 977 725
AgeI A'CCGG_T
Cuts at: 3835 3835
Size: 5447
AlwNI CAG_nnn'CTG
Cuts at: 968 2752 4319 968
Size: 1784 1567 2096
Fragments arranged by size:
2096 1784 1567
ApaLI G'TGCA_C
Cuts at: 770 4217 770
Size: 3447 2000
ApoI r'AATT_y
Cuts at: 381 463 1009 1424 1639 1735 1799 2437 2451
Size: 82 546 415 215 96 64 638 14
Cuts at: 2451 4800 5056 381
Size: 2349 256 772
Fragments arranged by size:
2349 772 638 546 415 256 215 96
82 64 14
AseI AT'TA_AT
Cuts at: 20 638 20
Size: 618 4829
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5447
AvaI C'yCGr_G
Cuts at: 2467 2467
Size: 5447
BamHI G'GATC_C
Cuts at: 2472 2472
Size: 5447
BanI G'GyrC_C
Cuts at: 2197 2463 2877 3125 3160 2197
Size: 266 414 248 35 4484
Fragments arranged by size:
4484 414 266 248 35
BanII G_rGCy'C
Cuts at: 1813 2065 2461 3491 1813
Size: 252 396 1030 3769
Fragments arranged by size:
3769 1030 396 252
BbeI G_GCGC'C
Cuts at: 2881 3129 2881
Size: 248 5199
BbsI GAAGACnn'nnnn_
Cuts at: 358 1046 1079 358
Size: 688 33 4726
Fragments arranged by size:
4726 688 33
BbvCI CC'TCA_GC
Cuts at: 1976 1976
Size: 5447
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1073 1107 1073
Size: 34 5413
BciVI GTATCCnnnnn_n'
Cuts at: 1339 3345 4106 5447 1339
Size: 2006 761 1341 1339
Fragments arranged by size:
2006 1341 1339 761
BclI T'GATC_A
Cuts at: 2967 2967
Size: 5447
BglI GCCn_nnn'nGGC
Cuts at: 706 706
Size: 5447
BglII A'GATC_T
Cuts at: 453 2962 4999 453
Size: 2509 2037 901
Fragments arranged by size:
2509 2037 901
BlpI GC'TnA_GC
Cuts at: 1723 2559 1723
Size: 836 4611
Bme1580I G_kGCm'C
Cuts at: 774 2165 3072 3165 4221 774
Size: 1391 907 93 1056 2000
Fragments arranged by size:
2000 1391 1056 907 93
BmgBI CAC'GTC
Cuts at: 2001 2075 2001
Size: 74 5373
BmrI ACTGGGnnnn_n'
Cuts at: 2016 2659 2740 3075 5219 2016
Size: 643 81 335 2144 2244
Fragments arranged by size:
2244 2144 643 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1122 3814 1122
Size: 2692 2755
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 843 1114 843
Size: 271 5176
Bpu10I CC'TnA_GC
Cuts at: 1676 1976 1676
Size: 300 5147
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2623 3502 3994 4292 2623
Size: 879 492 298 3778
Fragments arranged by size:
3778 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 293 1110 5151 293
Size: 817 4041 589
Fragments arranged by size:
4041 817 589
BsaAI yAC'GTr
Cuts at: 2800 2800
Size: 5447
BsaBI GATnn'nnATC
Cuts at: 6 2961 4998 6
Size: 2955 2037 455
Fragments arranged by size:
2955 2037 455
BsaHI Gr'CG_yC
Cuts at: 1945 2878 3126 3828 5324 1945
Size: 933 248 702 1496 2068
Fragments arranged by size:
2068 1496 933 702 248
BsaWI w'CCGG_w
Cuts at: 1420 1430 2636 3157 3835 4109 4256 4667 1420
Size: 10 1206 521 678 274 147 411 2200
Fragments arranged by size:
2200 1206 678 521 411 274 147 10
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 145 145
Size: 5447
BseYI C'CCAG_C
Cuts at: 2872 3630 4207 4703 2872
Size: 758 577 496 3616
Fragments arranged by size:
3616 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2015 2015
Size: 5447
BsiEI CG_ry'CG
Cuts at: 104 2989 3035 4243 104
Size: 2885 46 1208 1308
Fragments arranged by size:
2885 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 774 1420 1813 2461 3239 3429 4221 774
Size: 646 393 648 778 190 792 2000
Fragments arranged by size:
2000 792 778 648 646 393 190
Bsp1286I G_dGCh'C
Cuts at: 774 1420 1813 2065 2165 2461 3072 3165 3239
Size: 646 393 252 100 296 611 93 74
Cuts at: 3239 3429 3491 4221 774
Size: 190 62 730 2000
Fragments arranged by size:
2000 730 646 611 393 296 252 190
100 93 74 62
BspEI T'CCGG_A
Cuts at: 1420 4667 1420
Size: 3247 2200
BspHI T'CATG_A
Cuts at: 284 4623 284
Size: 4339 1108
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2163 2497 2711 3013 3394 2163
Size: 334 214 302 381 4216
Fragments arranged by size:
4216 381 334 302 214
BsrBI CCG'CTC
Cuts at: 50 3739 3793 5277 5442 50
Size: 3689 54 1484 165 55
Fragments arranged by size:
3689 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 245 948 1344 2137 3359 245
Size: 703 396 793 1222 2333
Fragments arranged by size:
2333 1222 793 703 396
BsrFI r'CCGG_y
Cuts at: 3445 3626 3835 3445
Size: 181 209 5057
Fragments arranged by size:
5057 209 181
BssSI C'ACGA_G
Cuts at: 3718 4076 3718
Size: 358 5089
BstAPI GCAn_nnn'nTGC
Cuts at: 2263 2824 2263
Size: 561 4886
BstBI TT'CG_AA
Cuts at: 488 3808 488
Size: 3320 2127
BstYI r'GATC_y
Cuts at: 1 453 762 1368 2472 2939 2962 3297 3543
Size: 452 309 606 1104 467 23 335 246
Cuts at: 3543 4555 4641 4653 4999 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1104 1012 606 467 452 449 346 335
309 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1045 1045
Size: 5447
BtgI C'CryG_G
Cuts at: 1372 1841 1372
Size: 469 4978
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3397 3578 3741 3397
Size: 181 163 5103
Fragments arranged by size:
5103 181 163
BtsI GCAGTG_nn'
Cuts at: 2690 2690
Size: 5447
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1610 1645 1610
Size: 35 5412
DraI TTT'AAA
Cuts at: 1499 2446 1499
Size: 947 4500
DraIII CAC_nnn'GTG
Cuts at: 1406 1406
Size: 5447
DrdI GACnn_nn'nnGTC
Cuts at: 3153 4011 3153
Size: 858 4589
EaeI y'GGCC_r
Cuts at: 3032 3206 3597 3624 4969 3032
Size: 174 391 27 1345 3510
Fragments arranged by size:
3510 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3032 3032
Size: 5447
EarI CTCTTCn'nnn_
Cuts at: 1818 2300 3470 3680 1818
Size: 482 1170 210 3585
Fragments arranged by size:
3585 1170 482 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3655 3965 4111 5257 3655
Size: 310 146 1146 3845
Fragments arranged by size:
3845 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1811 2459 1811
Size: 648 4799
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 141 160 418 616 843 1114 1297 1730 1892
Size: 19 258 198 227 271 183 433 162
Cuts at: 1892 1915 3272 3704 4451 4719 141
Size: 23 1357 432 747 268 869
Fragments arranged by size:
1357 869 747 433 432 271 268 258
227 198 183 162 23 19
EcoO109I rG'GnC_Cy
Cuts at: 2586 2586
Size: 5447
EcoRI G'AATT_C
Cuts at: 1424 1799 2451 1424
Size: 375 652 4420
Fragments arranged by size:
4420 652 375
EcoRV GAT'ATC
Cuts at: 922 922
Size: 5447
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 472 1847 3466 472
Size: 1375 1619 2453
Fragments arranged by size:
2453 1619 1375
FspI TGC'GCA
Cuts at: 3228 3228
Size: 5447
HaeII r_GCGC'y
Cuts at: 969 2881 3129 4151 5130 969
Size: 1912 248 1022 979 1286
Fragments arranged by size:
1912 1286 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 653 685 1222 1254 3289 3319 3321 3351 653
Size: 32 537 32 2035 30 2 30 2749
Fragments arranged by size:
2749 2035 537 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2109 2486 2109
Size: 377 5070
HindIII A'AGCT_T
Cuts at: 217 1501 217
Size: 1284 4163
KasI G'GCGC_C
Cuts at: 2877 3125 2877
Size: 248 5199
KpnI G_GTAC'C
Cuts at: 2467 2467
Size: 5447
MluI A'CGCG_T
Cuts at: 4880 4880
Size: 5447
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 630 758 1048 4118 4302 630
Size: 128 290 3070 184 1775
Fragments arranged by size:
3070 1775 290 184 128
MscI TGG'CCA
Cuts at: 3208 3208
Size: 5447
MslI CAynn'nnrTG
Cuts at: 112 910 2248 3436 3563 112
Size: 798 1338 1188 127 1996
Fragments arranged by size:
1996 1338 1188 798 127
MspA1I CmG'CkG
Cuts at: 1317 1346 2558 2872 3232 4245 4490 1317
Size: 29 1212 314 360 1013 245 2274
Fragments arranged by size:
2274 1212 1013 360 314 245 29
NaeI GCC'GGC
Cuts at: 3628 3628
Size: 5447
NarI GG'CG_CC
Cuts at: 2878 3126 2878
Size: 248 5199
NdeI CA'TA_TG
Cuts at: 1950 1950
Size: 5447
NgoMIV G'CCGG_C
Cuts at: 3626 3626
Size: 5447
NspI r_CATG'y
Cuts at: 1128 2212 2500 3000 3907 1128
Size: 1084 288 500 907 2668
Fragments arranged by size:
2668 1084 907 500 288
PciI A'CATG_T
Cuts at: 3903 3903
Size: 5447
PfoI T'CCnGG_A
Cuts at: 1923 1923
Size: 5447
PmeI GTTT'AAAC
Cuts at: 2446 2446
Size: 5447
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4586 4618 4586
Size: 32 5415
PsiI TTA'TAA
Cuts at: 1531 1531
Size: 5447
PstI C_TGCA'G
Cuts at: 2494 3179 2494
Size: 685 4762
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5447
PvuII CAG'CTG
Cuts at: 1346 2872 3232 1346
Size: 1526 360 3561
Fragments arranged by size:
3561 1526 360
RsrII CG'GwC_CG
Cuts at: 3642 3642
Size: 5447
SacI G_AGCT'C
Cuts at: 1813 2461 1813
Size: 648 4799
SalI G'TCGA_C
Cuts at: 2484 2484
Size: 5447
SapI GCTCTTCn'nnn_
Cuts at: 3470 3680 3470
Size: 210 5237
SbfI CC_TGCA'GG
Cuts at: 2494 2494
Size: 5447
ScaI AGT'ACT
Cuts at: 4964 4964
Size: 5447
SfcI C'TryA_G
Cuts at: 33 2089 2490 3175 4168 4359 33
Size: 2056 401 685 993 191 1121
Fragments arranged by size:
2056 1121 993 685 401 191
SfoI GGC'GCC
Cuts at: 2879 3127 2879
Size: 248 5199
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5447
SmaI CCC'GGG
Cuts at: 2469 2469
Size: 5447
SmlI C'TyrA_G
Cuts at: 2602 3517 4009 4271 2602
Size: 915 492 262 3778
Fragments arranged by size:
3778 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5447
SphI G_CATG'C
Cuts at: 1128 2500 3000 1128
Size: 1372 500 3575
Fragments arranged by size:
3575 1372 500
SspI AAT'ATT
Cuts at: 1654 1654
Size: 5447
StyI C'CwwG_G
Cuts at: 1437 2124 2581 2935 1437
Size: 687 457 354 3949
Fragments arranged by size:
3949 687 457 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3030 3163 3622 3831 3030
Size: 133 459 209 4646
Fragments arranged by size:
4646 459 209 133
TatI w'GTAC_w
Cuts at: 537 4962 537
Size: 4425 1022
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4964 4996 4964
Size: 32 5415
Tth111I GACn'n_nGTC
Cuts at: 1753 3244 1753
Size: 1491 3956
XbaI T'CTAG_A
Cuts at: 2478 2478
Size: 5447
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1330 1389 2942 1330
Size: 59 1553 3835
Fragments arranged by size:
3835 1553 59
XmaI C'CCGG_G
Cuts at: 2467 2467
Size: 5447
XmnI GAAnn'nnTTC
Cuts at: 484 484
Size: 5447
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NdeI NgoMIV NspI PciI
PfoI PmeI PpiI PsiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BfrBI BmtI BsaXI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I ClaI
EcoNI FseI FspAI HpaI MfeI NcoI NheI NotI
NruI NsiI PacI PasI PflMI PmlI PpuMI PshAI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI StuI SwaI XhoI ZraI