Restriction map for
pF1KA0051
(Circular) (Six-base) MAPSORT of: pF1KA0051.seq Check: 4399 from: 1 to: 8084
pF1KA0051 8084 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2455 2738 4665 2455
Size: 283 1927 5874
Fragments arranged by size:
5874 1927 283
AatII G_ACGT'C
Cuts at: 216 216
Size: 8084
AccI GT'mk_AC
Cuts at: 335 5122 335
Size: 4787 3297
Acc65I G'GTAC_C
Cuts at: 4589 5100 4589
Size: 511 7573
AclI AA'CG_TT
Cuts at: 4275 7924 4275
Size: 3649 4435
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1639 2135 2443 3628 3679 3787 3916 5909 6341
Size: 496 308 1185 51 108 129 1993 432
Cuts at: 6341 6694 7356 1639
Size: 353 662 2367
Fragments arranged by size:
2367 1993 1185 662 496 432 353 308
129 108 51
AfeI AGC'GCT
Cuts at: 2578 2578
Size: 8084
AflII C'TTAA_G
Cuts at: 3849 3849
Size: 8084
AflIII A'CryG_T
Cuts at: 3143 5436 7236 7517 3143
Size: 2293 1800 281 3710
Fragments arranged by size:
3710 2293 1800 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 709 4403 709
Size: 3694 4390
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2372 2404 2372
Size: 32 8052
AlwNI CAG_nnn'CTG
Cuts at: 1158 1558 2550 2823 3030 4548 4971 5389 6827
Size: 400 992 273 207 1518 423 418 1438
Cuts at: 6827 1158
Size: 2415
Fragments arranged by size:
2415 1518 1438 992 423 418 400 273
207
ApaLI G'TGCA_C
Cuts at: 6922 6922
Size: 8084
ApoI r'AATT_y
Cuts at: 800 992 1648 1922 2241 2253 2398 2415 2719
Size: 192 656 274 319 12 145 17 304
Cuts at: 2719 3889 3940 4924 5088 7437 7693 800
Size: 1170 51 984 164 2349 256 1191
Fragments arranged by size:
2349 1191 1170 984 656 319 304 274
256 192 164 145 51 17 12
AseI AT'TA_AT
Cuts at: 20 779 1292 1742 1997 3860 20
Size: 759 513 450 255 1863 4244
Fragments arranged by size:
4244 1863 759 513 450 255
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8084
AvaI C'yCGr_G
Cuts at: 2270 2660 5104 2270
Size: 390 2444 5250
Fragments arranged by size:
5250 2444 390
AvrII C'CTAG_G
Cuts at: 279 4750 279
Size: 4471 3613
BamHI G'GATC_C
Cuts at: 2694 5109 2694
Size: 2415 5669
BanI G'GyrC_C
Cuts at: 3289 4107 4589 5100 5514 5762 5797 3289
Size: 818 482 511 414 248 35 5576
Fragments arranged by size:
5576 818 511 482 414 248 35
BanII G_rGCy'C
Cuts at: 866 1089 1457 3787 5098 6128 866
Size: 223 368 2330 1311 1030 2822
Fragments arranged by size:
2822 2330 1311 1030 368 223
BbeI G_GCGC'C
Cuts at: 5518 5766 5518
Size: 248 7836
BbsI GAAGACnn'nnnn_
Cuts at: 670 1330 3496 4451 670
Size: 660 2166 955 4303
Fragments arranged by size:
4303 2166 955 660
BbvCI CC'TCA_GC
Cuts at: 2690 2690
Size: 8084
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 799 825 833 859 2251 2285 2551 2585 799
Size: 26 8 26 1392 34 266 34 6298
Fragments arranged by size:
6298 1392 266 34 34 26 26 8
BciVI GTATCCnnnnn_n'
Cuts at: 571 1382 2374 2464 5982 7038 8084 571
Size: 811 992 90 3518 1056 1046 571
Fragments arranged by size:
3518 1056 1046 992 811 571 90
BclI T'GATC_A
Cuts at: 1493 2351 4550 5604 1493
Size: 858 2199 1054 3973
Fragments arranged by size:
3973 2199 1054 858
BglII A'GATC_T
Cuts at: 288 3317 5599 7636 288
Size: 3029 2282 2037 736
Fragments arranged by size:
3029 2282 2037 736
BlpI GC'TnA_GC
Cuts at: 902 5196 902
Size: 4294 3790
Bme1580I G_kGCm'C
Cuts at: 2043 3294 4112 5709 5802 6926 2043
Size: 1251 818 1597 93 1124 3201
Fragments arranged by size:
3201 1597 1251 1124 818 93
BmgBI CAC'GTC
Cuts at: 4218 4387 4218
Size: 169 7915
BmrI ACTGGGnnnn_n'
Cuts at: 358 2181 3322 3870 5296 5377 5712 7856 358
Size: 1823 1141 548 1426 81 335 2144 586
Fragments arranged by size:
2144 1823 1426 1141 586 548 335 81
BmtI G_CTAG'C
Cuts at: 3475 3475
Size: 8084
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6451 6451
Size: 8084
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 331 1247 2227 2845 4244 331
Size: 916 980 618 1399 4171
Fragments arranged by size:
4171 1399 980 916 618
Bpu10I CC'TnA_GC
Cuts at: 322 1625 2690 3461 322
Size: 1303 1065 771 4945
Fragments arranged by size:
4945 1303 1065 771
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1831 2965 5260 6139 6853 7151 1831
Size: 1134 2295 879 714 298 2764
Fragments arranged by size:
2764 2295 1134 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 2203 2983 4306 7788 2203
Size: 780 1323 3482 2499
Fragments arranged by size:
3482 2499 1323 780
BsaAI yAC'GTr
Cuts at: 5437 5437
Size: 8084
BsaBI GATnn'nnATC
Cuts at: 6 1391 5598 7635 6
Size: 1385 4207 2037 455
Fragments arranged by size:
4207 2037 1385 455
BsaHI Gr'CG_yC
Cuts at: 213 5515 5763 6465 7961 213
Size: 5302 248 702 1496 336
Fragments arranged by size:
5302 1496 702 336 248
BsaWI w'CCGG_w
Cuts at: 4059 4295 5273 5794 6472 6883 7030 7304 4059
Size: 236 978 521 678 411 147 274 4839
Fragments arranged by size:
4839 978 678 521 411 274 236 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1207 1237 1462 1492 2500 2530 4985 5015 1207
Size: 30 225 30 1008 30 2455 30 4276
Fragments arranged by size:
4276 2455 1008 225 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 208 328 985 1228 2290 2776 3175 4614 208
Size: 120 657 243 1062 486 399 1439 3678
Fragments arranged by size:
3678 1439 1062 657 486 399 243 120
BseYI C'CCAG_C
Cuts at: 135 1253 1355 1835 5509 6267 6932 7340 135
Size: 1118 102 480 3674 758 665 408 879
Fragments arranged by size:
3674 1118 879 758 665 480 408 102
BsiEI CG_ry'CG
Cuts at: 104 151 5626 5672 6902 104
Size: 47 5475 46 1230 1286
Fragments arranged by size:
5475 1286 1230 47 46
BsiHKAI G_wGCw'C
Cuts at: 1457 4067 5098 5876 6066 6926 1457
Size: 2610 1031 778 190 860 2615
Fragments arranged by size:
2615 2610 1031 860 778 190
BsmI GAATG_Cn'
Cuts at: 474 843 2450 3309 4239 474
Size: 369 1607 859 930 4319
Fragments arranged by size:
4319 1607 930 859 369
BsmBI CGTCTCn'nnnn_
Cuts at: 204 4377 204
Size: 4173 3911
Bsp1286I G_dGCh'C
Cuts at: 866 1089 1457 2043 3294 3787 4067 4112 5098
Size: 223 368 586 1251 493 280 45 986
Cuts at: 5098 5709 5802 5876 6066 6128 6926 866
Size: 611 93 74 190 62 798 2024
Fragments arranged by size:
2024 1251 986 798 611 586 493 368
280 223 190 93 74 62 45
BspEI T'CCGG_A
Cuts at: 4059 4059
Size: 8084
BspHI T'CATG_A
Cuts at: 3477 5003 6516 3477
Size: 1526 1513 5045
Fragments arranged by size:
5045 1526 1513
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2455 2519 2738 3176 4665 4714 4853 4979 5134
Size: 64 219 438 1489 49 139 126 155
Cuts at: 5134 5348 5650 6031 2455
Size: 214 302 381 4508
Fragments arranged by size:
4508 1489 438 381 302 219 214 155
139 126 64 49
BsrBI CCG'CTC
Cuts at: 50 6376 6430 7914 8079 50
Size: 6326 54 1484 165 55
Fragments arranged by size:
6326 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3593 3788 4052 4559 5996 3593
Size: 195 264 507 1437 5681
Fragments arranged by size:
5681 1437 507 264 195
BsrFI r'CCGG_y
Cuts at: 6082 6263 6082
Size: 181 7903
BssSI C'ACGA_G
Cuts at: 6355 7063 6355
Size: 708 7376
BstAPI GCAn_nnn'nTGC
Cuts at: 760 1246 5461 760
Size: 486 4215 3383
Fragments arranged by size:
4215 3383 486
BstBI TT'CG_AA
Cuts at: 4910 6445 4910
Size: 1535 6549
BstXI CCAn_nnnn'nTGG
Cuts at: 309 3063 3111 4929 309
Size: 2754 48 1818 3464
Fragments arranged by size:
3464 2754 1818 48
BstYI r'GATC_y
Cuts at: 1 288 2279 2694 3317 4481 5109 5576 5599
Size: 287 1991 415 623 1164 628 467 23
Cuts at: 5599 5934 6180 6486 6498 6584 7636 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1991 1164 1052 628 623 467 449 415
335 306 287 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1980 1980
Size: 8084
BtgI C'CryG_G
Cuts at: 473 2376 3285 3294 4520 473
Size: 1903 909 9 1226 4037
Fragments arranged by size:
4037 1903 1226 909 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 6034 6215 6378 6034
Size: 181 163 7740
Fragments arranged by size:
7740 181 163
BtsI GCAGTG_nn'
Cuts at: 1472 5327 1472
Size: 3855 4229
ClaI AT'CG_AT
Cuts at: 3687 3687
Size: 8084
DraI TTT'AAA
Cuts at: 4151 4496 4863 5083 4151
Size: 345 367 220 7152
Fragments arranged by size:
7152 367 345 220
DraIII CAC_nnn'GTG
Cuts at: 2038 2038
Size: 8084
DrdI GACnn_nn'nnGTC
Cuts at: 1187 4188 5790 7134 1187
Size: 3001 1602 1344 2137
Fragments arranged by size:
3001 2137 1602 1344
EaeI y'GGCC_r
Cuts at: 148 2336 3065 3297 5669 5843 6234 6261 7606
Size: 2188 729 232 2372 174 391 27 1345
Cuts at: 7606 148
Size: 626
Fragments arranged by size:
2372 2188 1345 729 626 391 232 174
27
EagI C'GGCC_G
Cuts at: 148 5669 148
Size: 5521 2563
EarI CTCTTCn'nnn_
Cuts at: 253 854 1171 1861 2783 2995 4466 6107 6317
Size: 601 317 690 922 212 1471 1641 210
Cuts at: 6317 253
Size: 2020
Fragments arranged by size:
2020 1641 1471 922 690 601 317 212
210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 102 6292 7034 7180 7894 102
Size: 6190 742 146 714 292
Fragments arranged by size:
6190 742 714 292 146
EcoICRI GAG'CTC
Cuts at: 1455 5096 1455
Size: 3641 4443
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 331 1247 1639 2135 2227 2443 2845 3628 3679
Size: 916 392 496 92 216 402 783 51
Cuts at: 3679 3787 3916 4244 5909 6341 6694 7356 331
Size: 108 129 328 1665 432 353 662 1059
Fragments arranged by size:
1665 1059 916 783 662 496 432 402
392 353 328 216 129 108 92 51
EcoNI CCTnn'n_nnAGG
Cuts at: 1080 1207 4102 1080
Size: 127 2895 5062
Fragments arranged by size:
5062 2895 127
EcoO109I rG'GnC_Cy
Cuts at: 1785 4842 4968 5223 1785
Size: 3057 126 255 4646
Fragments arranged by size:
4646 3057 255 126
EcoRI G'AATT_C
Cuts at: 800 1648 2398 5088 800
Size: 848 750 2690 3796
Fragments arranged by size:
3796 2690 848 750
EcoRV GAT'ATC
Cuts at: 522 1668 2850 522
Size: 1146 1182 5756
Fragments arranged by size:
5756 1182 1146
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 651 1995 4677 4843 6103 651
Size: 1344 2682 166 1260 2632
Fragments arranged by size:
2682 2632 1344 1260 166
FspI TGC'GCA
Cuts at: 5865 5865
Size: 8084
HaeII r_GCGC'y
Cuts at: 2580 3472 5518 5766 6996 7767 2580
Size: 892 2046 248 1230 771 2897
Fragments arranged by size:
2897 2046 1230 892 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1207 1239 1462 1494 3234 3266 3340 3372 3402
Size: 32 223 32 1740 32 74 32 30
Cuts at: 3402 3434 3943 3975 4376 4408 4985 5017 5926
Size: 32 509 32 401 32 577 32 909
Cuts at: 5926 5956 5958 5988 1207
Size: 30 2 30 3303
Fragments arranged by size:
3303 1740 909 577 509 401 223 74
32 32 32 32 32 32 32 32
30 30 30 2
HincII GTy'rAC
Cuts at: 129 633 5037 5123 129
Size: 504 4404 86 3090
Fragments arranged by size:
4404 3090 504 86
HindIII A'AGCT_T
Cuts at: 1673 3017 1673
Size: 1344 6740
KasI G'GCGC_C
Cuts at: 5514 5762 5514
Size: 248 7836
KpnI G_GTAC'C
Cuts at: 4593 5104 4593
Size: 511 7573
MfeI C'AATT_G
Cuts at: 1540 1540
Size: 8084
MluI A'CGCG_T
Cuts at: 3143 7517 3143
Size: 4374 3710
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 653 1895 1970 2993 3173 4016 4881 4912 6843
Size: 1242 75 1023 180 843 865 31 1931
Cuts at: 6843 7027 653
Size: 184 1710
Fragments arranged by size:
1931 1710 1242 1023 865 843 184 180
75 31
MscI TGG'CCA
Cuts at: 2338 3067 3299 5845 2338
Size: 729 232 2546 4577
Fragments arranged by size:
4577 2546 729 232
MslI CAynn'nnrTG
Cuts at: 691 2525 2615 4046 6073 6200 691
Size: 1834 90 1431 2027 127 2575
Fragments arranged by size:
2575 2027 1834 1431 127 90
MspA1I CmG'CkG
Cuts at: 1359 1903 2328 2823 4584 5195 5509 5869 6653
Size: 544 425 495 1761 611 314 360 784
Cuts at: 6653 6898 1359
Size: 245 2545
Fragments arranged by size:
2545 1761 784 611 544 495 425 360
314 245
NaeI GCC'GGC
Cuts at: 6265 6265
Size: 8084
NarI GG'CG_CC
Cuts at: 5515 5763 5515
Size: 248 7836
NcoI C'CATG_G
Cuts at: 473 473
Size: 8084
NgoMIV G'CCGG_C
Cuts at: 6263 6263
Size: 8084
NheI G'CTAG_C
Cuts at: 3471 3471
Size: 8084
NspI r_CATG'y
Cuts at: 278 543 763 1636 3522 3811 5137 5637 7240
Size: 265 220 873 1886 289 1326 500 1603
Cuts at: 7240 278
Size: 1122
Fragments arranged by size:
1886 1603 1326 1122 873 500 289 265
220
PacI TTA_AT'TAA
Cuts at: 1742 1742
Size: 8084
PasI CC'CwG_GG
Cuts at: 1104 1104
Size: 8084
PciI A'CATG_T
Cuts at: 7236 7236
Size: 8084
PflMI CCAn_nnn'nTGG
Cuts at: 2205 2733 2823 2205
Size: 528 90 7466
Fragments arranged by size:
7466 528 90
PfoI T'CCnGG_A
Cuts at: 167 1889 2407 2603 167
Size: 1722 518 196 5648
Fragments arranged by size:
5648 1722 518 196
PmeI GTTT'AAAC
Cuts at: 5083 5083
Size: 8084
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 346 378 1424 1456 6530 6562 346
Size: 32 1046 32 5074 32 1868
Fragments arranged by size:
5074 1868 1046 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 4842 4968 4842
Size: 126 7958
PstI C_TGCA'G
Cuts at: 192 1094 1124 1208 1223 1418 1784 1803 2366
Size: 902 30 84 15 195 366 19 563
Cuts at: 2366 2597 4979 5131 5816 192
Size: 231 2382 152 685 2460
Fragments arranged by size:
2460 2382 902 685 563 366 231 195
152 84 30 19 15
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8084
PvuII CAG'CTG
Cuts at: 1359 1903 2328 2823 5509 5869 1359
Size: 544 425 495 2686 360 3574
Fragments arranged by size:
3574 2686 544 495 425 360
RsrII CG'GwC_CG
Cuts at: 6279 6279
Size: 8084
SacI G_AGCT'C
Cuts at: 1457 5098 1457
Size: 3641 4443
SalI G'TCGA_C
Cuts at: 5121 5121
Size: 8084
SapI GCTCTTCn'nnn_
Cuts at: 854 6107 6317 854
Size: 5253 210 2621
Fragments arranged by size:
5253 2621 210
SbfI CC_TGCA'GG
Cuts at: 5131 5131
Size: 8084
ScaI AGT'ACT
Cuts at: 2599 3958 7601 2599
Size: 1359 3643 3082
Fragments arranged by size:
3643 3082 1359
SexAI A'CCwGG_T
Cuts at: 1694 1694
Size: 8084
SfcI C'TryA_G
Cuts at: 33 188 515 1090 1120 1204 1219 1414 1780
Size: 155 327 575 30 84 15 195 366
Cuts at: 1780 1789 1799 2362 2593 4975 4981 5127 5812
Size: 9 10 563 231 2382 6 146 685
Cuts at: 5812 6780 6971 33
Size: 968 191 1146
Fragments arranged by size:
2382 1146 968 685 575 563 366 327
231 195 191 155 146 84 30 15
10 9 6
SfoI GGC'GCC
Cuts at: 5516 5764 5516
Size: 248 7836
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8084
SmaI CCC'GGG
Cuts at: 5106 5106
Size: 8084
SmlI C'TyrA_G
Cuts at: 1810 2980 3849 5239 6154 6868 7130 1810
Size: 1170 869 1390 915 714 262 2764
Fragments arranged by size:
2764 1390 1170 915 869 714 262
SpeI A'CTAG_T
Cuts at: 67 4614 67
Size: 4547 3537
SphI G_CATG'C
Cuts at: 278 3811 5137 5637 278
Size: 3533 1326 500 2725
Fragments arranged by size:
3533 2725 1326 500
SspI AAT'ATT
Cuts at: 1572 1572
Size: 8084
StuI AGG'CCT
Cuts at: 596 2348 596
Size: 1752 6332
StyI C'CwwG_G
Cuts at: 279 473 866 4750 5218 5572 279
Size: 194 393 3884 468 354 2791
Fragments arranged by size:
3884 2791 468 393 354 194
SwaI ATTT'AAAT
Cuts at: 4151 4151
Size: 8084
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1459 1463 1972 2151 4036 5667 5800 6259 6468
Size: 4 509 179 1885 1631 133 459 209
Cuts at: 6468 1459
Size: 3075
Fragments arranged by size:
3075 1885 1631 509 459 209 179 133
4
TatI w'GTAC_w
Cuts at: 2597 3956 7599 2597
Size: 1359 3643 3082
Fragments arranged by size:
3643 3082 1359
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2852 2884 7601 7633 2852
Size: 32 4717 32 3303
Fragments arranged by size:
4717 3303 32 32
Tth111I GACn'n_nGTC
Cuts at: 1424 5881 1424
Size: 4457 3627
XbaI T'CTAG_A
Cuts at: 5115 5115
Size: 8084
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 306 3108 5579 306
Size: 2802 2471 2811
Fragments arranged by size:
2811 2802 2471
XmaI C'CCGG_G
Cuts at: 5104 5104
Size: 8084
XmnI GAAnn'nnTTC
Cuts at: 358 358
Size: 8084
ZraI GAC'GTC
Cuts at: 214 214
Size: 8084
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AlfI AloI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NheI NspI PacI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StuI StyI SwaI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
AgeI AhdI AleI ApaI AscI BaeI BfrBI BglI
BsgI BsiWI BsrGI BssHII BstEII BstZ17I CspCI FseI
FspAI HpaI NdeI NotI NruI NsiI PmlI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI XhoI