Restriction map for
pF1KA0054
(Circular) (Six-base) MAPSORT of: pF1KA0054.seq Check: 507 from: 1 to: 8939
pF1KA0054 8939 bp
With 182 enzymes: SgfI *
August 5, 2008 16:25 ..
AccI GT'mk_AC
Cuts at: 348 4602 5977 348
Size: 4254 1375 3310
Fragments arranged by size:
4254 3310 1375
Acc65I G'GTAC_C
Cuts at: 5955 5955
Size: 8939
AclI AA'CG_TT
Cuts at: 1320 2131 8779 1320
Size: 811 6648 1480
Fragments arranged by size:
6648 1480 811
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 692 1682 1864 5441 5452 5659 6764 7196 7549
Size: 990 182 3577 11 207 1105 432 353
Cuts at: 7549 8211 692
Size: 662 1420
Fragments arranged by size:
3577 1420 1105 990 662 432 353 207
182 11
AfeI AGC'GCT
Cuts at: 5802 5802
Size: 8939
AflII C'TTAA_G
Cuts at: 157 157
Size: 8939
AflIII A'CryG_T
Cuts at: 351 1142 2895 6291 8091 8372 351
Size: 791 1753 3396 1800 281 918
Fragments arranged by size:
3396 1800 1753 918 791 281
AhdI GACnn_n'nnGTC
Cuts at: 5865 5865
Size: 8939
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 420 2681 420
Size: 2261 6678
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2692 2724 2692
Size: 32 8907
AlwNI CAG_nnn'CTG
Cuts at: 919 1566 4447 5883 6244 7682 919
Size: 647 2881 1436 361 1438 2176
Fragments arranged by size:
2881 2176 1438 1436 647 361
ApaI G_GGCC'C
Cuts at: 5814 5814
Size: 8939
ApaLI G'TGCA_C
Cuts at: 7777 7777
Size: 8939
ApoI r'AATT_y
Cuts at: 840 1406 3292 3424 3624 4096 5943 8292 8548
Size: 566 1886 132 200 472 1847 2349 256
Cuts at: 8548 840
Size: 1231
Fragments arranged by size:
2349 1886 1847 1231 566 472 256 200
132
AseI AT'TA_AT
Cuts at: 20 3980 20
Size: 3960 4979
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8939
AvaI C'yCGr_G
Cuts at: 3434 4569 5653 5959 3434
Size: 1135 1084 306 6414
Fragments arranged by size:
6414 1135 1084 306
AvrII C'CTAG_G
Cuts at: 622 5889 622
Size: 5267 3672
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1052 1085 3744 3777 1052
Size: 33 2659 33 6214
Fragments arranged by size:
6214 2659 33 33
BamHI G'GATC_C
Cuts at: 2022 5566 5964 2022
Size: 3544 398 4997
Fragments arranged by size:
4997 3544 398
BanI G'GyrC_C
Cuts at: 694 3417 3726 5139 5955 6369 6617 6652 694
Size: 2723 309 1413 816 414 248 35 2981
Fragments arranged by size:
2981 2723 1413 816 414 309 248 35
BanII G_rGCy'C
Cuts at: 3441 5357 5767 5814 5899 5953 6983 3441
Size: 1916 410 47 85 54 1030 5397
Fragments arranged by size:
5397 1916 1030 410 85 54 47
BbeI G_GCGC'C
Cuts at: 6373 6621 6373
Size: 248 8691
BbsI GAAGACnn'nnnn_
Cuts at: 119 277 581 3400 3496 119
Size: 158 304 2819 96 5562
Fragments arranged by size:
5562 2819 304 158 96
BbvCI CC'TCA_GC
Cuts at: 4295 4343 4358 4739 4295
Size: 48 15 381 8495
Fragments arranged by size:
8495 381 48 15
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 258 292 4654 4688 258
Size: 34 4362 34 4509
Fragments arranged by size:
4509 4362 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 450 3745 6837 7893 8939 450
Size: 3295 3092 1056 1046 450
Fragments arranged by size:
3295 3092 1056 1046 450
BclI T'GATC_A
Cuts at: 2571 2979 3522 4866 6459 2571
Size: 408 543 1344 1593 5051
Fragments arranged by size:
5051 1593 1344 543 408
BfrBI ATG'CAT
Cuts at: 1287 1287
Size: 8939
BglI GCCn_nnn'nGGC
Cuts at: 394 5229 394
Size: 4835 4104
BglII A'GATC_T
Cuts at: 1269 6454 8491 1269
Size: 5185 2037 1717
Fragments arranged by size:
5185 2037 1717
BlpI GC'TnA_GC
Cuts at: 5582 6051 5582
Size: 469 8470
Bme1580I G_kGCm'C
Cuts at: 699 5814 6564 6657 7781 699
Size: 5115 750 93 1124 1857
Fragments arranged by size:
5115 1857 1124 750 93
BmgBI CAC'GTC
Cuts at: 4069 4069
Size: 8939
BmrI ACTGGGnnnn_n'
Cuts at: 693 1512 2317 2427 3363 3680 4082 6151 6232
Size: 819 805 110 936 317 402 2069 81
Cuts at: 6232 6567 8711 693
Size: 335 2144 921
Fragments arranged by size:
2144 2069 936 921 819 805 402 335
317 110 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4584 4634 7306 4584
Size: 50 2672 6217
Fragments arranged by size:
6217 2672 50
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1616 4711 1616
Size: 3095 5844
Bpu10I CC'TnA_GC
Cuts at: 2144 4295 4343 4358 4739 5837 2144
Size: 2151 48 15 381 1098 5246
Fragments arranged by size:
5246 2151 1098 381 48 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 169 2334 4812 5423 6115 6994 7708 8006 169
Size: 2165 2478 611 692 879 714 298 1102
Fragments arranged by size:
2478 2165 1102 879 714 692 611 298
BsaI GGTCTCn'nnnn_
Cuts at: 4810 8643 4810
Size: 3833 5106
BsaAI yAC'GTr
Cuts at: 570 1143 2998 3097 6292 570
Size: 573 1855 99 3195 3217
Fragments arranged by size:
3217 3195 1855 573 99
BsaBI GATnn'nnATC
Cuts at: 6 1013 6453 8490 6
Size: 1007 5440 2037 455
Fragments arranged by size:
5440 2037 1007 455
BsaHI Gr'CG_yC
Cuts at: 6370 6618 7320 8816 6370
Size: 248 702 1496 6493
Fragments arranged by size:
6493 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3967 4455 6128 6649 7327 7738 7885 8159 3967
Size: 488 1673 521 678 411 147 274 4747
Fragments arranged by size:
4747 1673 678 521 488 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 875 905 2692 2722 2819 2849 3698 3728 5048
Size: 30 1787 30 97 30 849 30 1320
Cuts at: 5048 5078 875
Size: 30 4736
Fragments arranged by size:
4736 1787 1320 849 97 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1522 2840 3587 4346 4424 5111 5426 1522
Size: 1318 747 759 78 687 315 5035
Fragments arranged by size:
5035 1318 759 747 687 315 78
BseYI C'CCAG_C
Cuts at: 357 2303 4199 5046 6364 7122 7787 8195 357
Size: 1946 1896 847 1318 758 665 408 1101
Fragments arranged by size:
1946 1896 1318 1101 847 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 177 4430 177
Size: 4253 4686
BsiEI CG_ry'CG
Cuts at: 104 6481 6527 7757 104
Size: 6377 46 1230 1286
Fragments arranged by size:
6377 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 899 3231 3274 5767 5899 5953 6731 6921 7781
Size: 2332 43 2493 132 54 778 190 860
Cuts at: 7781 899
Size: 2057
Fragments arranged by size:
2493 2332 2057 860 778 190 132 54
43
BsmI GAATG_Cn'
Cuts at: 986 1834 3453 986
Size: 848 1619 6472
Fragments arranged by size:
6472 1619 848
Bsp1286I G_dGCh'C
Cuts at: 699 899 3231 3274 3441 5357 5767 5814 5899
Size: 200 2332 43 167 1916 410 47 85
Cuts at: 5899 5953 6564 6657 6731 6921 6983 7781 699
Size: 54 611 93 74 190 62 798 1857
Fragments arranged by size:
2332 1916 1857 798 611 410 200 190
167 93 85 74 62 54 47 43
BspEI T'CCGG_A
Cuts at: 3967 4455 3967
Size: 488 8451
BspHI T'CATG_A
Cuts at: 3321 7371 3321
Size: 4050 4889
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3702 5989 6203 6505 6886 3702
Size: 2287 214 302 381 5755
Fragments arranged by size:
5755 2287 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2708 7231 7285 8769 8934 50
Size: 2658 4523 54 1484 165 55
Fragments arranged by size:
4523 2658 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 997 1034 3260 3809 4508 5218 5568 5916 6851
Size: 37 2226 549 699 710 350 348 935
Cuts at: 6851 997
Size: 3085
Fragments arranged by size:
3085 2226 935 710 699 549 350 348
37
BsrFI r'CCGG_y
Cuts at: 6937 7118 6937
Size: 181 8758
BsrGI T'GTAC_A
Cuts at: 2835 5250 2835
Size: 2415 6524
BssSI C'ACGA_G
Cuts at: 2822 7210 7918 2822
Size: 4388 708 3843
Fragments arranged by size:
4388 3843 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1045 1609 4165 6316 1045
Size: 564 2556 2151 3668
Fragments arranged by size:
3668 2556 2151 564
BstBI TT'CG_AA
Cuts at: 3009 3990 7300 3009
Size: 981 3310 4648
Fragments arranged by size:
4648 3310 981
BstXI CCAn_nnnn'nTGG
Cuts at: 2930 3538 2930
Size: 608 8331
BstYI r'GATC_y
Cuts at: 1 1269 2022 3660 3844 5566 5964 6431 6454
Size: 1268 753 1638 184 1722 398 467 23
Cuts at: 6454 6789 7035 7341 7353 7439 8491 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1722 1638 1268 1052 753 467 449 398
335 306 246 184 86 23 12
BstZ17I GTA'TAC
Cuts at: 4603 4603
Size: 8939
Bsu36I CC'TnA_GG
Cuts at: 5627 5627
Size: 8939
BtgI C'CryG_G
Cuts at: 107 227 1987 2504 3742 107
Size: 120 1760 517 1238 5304
Fragments arranged by size:
5304 1760 1238 517 120
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 6889 7070 7233 6889
Size: 181 163 8595
Fragments arranged by size:
8595 181 163
BtsI GCAGTG_nn'
Cuts at: 189 404 4663 4803 6182 189
Size: 215 4259 140 1379 2946
Fragments arranged by size:
4259 2946 1379 215 140
ClaI AT'CG_AT
Cuts at: 1896 1896
Size: 8939
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 432 467 2058 2093 432
Size: 35 1591 35 7278
Fragments arranged by size:
7278 1591 35 35
DraI TTT'AAA
Cuts at: 5931 5938 5931
Size: 7 8932
DrdI GACnn_nn'nnGTC
Cuts at: 6645 7989 6645
Size: 1344 7595
EaeI y'GGCC_r
Cuts at: 216 371 2060 2765 2920 4423 5697 5741 6524
Size: 155 1689 705 155 1503 1274 44 783
Cuts at: 6524 6698 7089 7116 8461 216
Size: 174 391 27 1345 694
Fragments arranged by size:
1689 1503 1345 1274 783 705 694 391
174 155 155 44 27
EagI C'GGCC_G
Cuts at: 6524 6524
Size: 8939
EarI CTCTTCn'nnn_
Cuts at: 114 1378 2897 5339 5845 5878 6962 7172 114
Size: 1264 1519 2442 506 33 1084 210 1881
Fragments arranged by size:
2442 1881 1519 1264 1084 506 210 33
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 601 7147 7889 8035 8749 601
Size: 6546 742 146 714 791
Fragments arranged by size:
6546 791 742 714 146
EcoICRI GAG'CTC
Cuts at: 5765 5897 5951 5765
Size: 132 54 8753
Fragments arranged by size:
8753 132 54
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 692 1616 1682 1864 4711 5441 5452 5659 6764
Size: 924 66 182 2847 730 11 207 1105
Cuts at: 6764 7196 7549 8211 692
Size: 432 353 662 1420
Fragments arranged by size:
2847 1420 1105 924 730 662 432 353
207 182 66 11
EcoO109I rG'GnC_Cy
Cuts at: 201 244 4384 5362 5811 5828 6078 201
Size: 43 4140 978 449 17 250 3062
Fragments arranged by size:
4140 3062 978 449 250 43 17
EcoRI G'AATT_C
Cuts at: 840 5943 840
Size: 5103 3836
EcoRV GAT'ATC
Cuts at: 565 565
Size: 8939
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 770 2410 3125 3166 6958 770
Size: 1640 715 41 3792 2751
Fragments arranged by size:
3792 2751 1640 715 41
FspI TGC'GCA
Cuts at: 6720 6720
Size: 8939
HaeII r_GCGC'y
Cuts at: 5064 5605 5804 6373 6621 7851 8622 5064
Size: 541 199 569 248 1230 771 5381
Fragments arranged by size:
5381 1230 771 569 541 248 199
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 574 606 942 974 1318 1350 1458 1490 1889
Size: 32 336 32 344 32 108 32 399
Cuts at: 1889 1921 4021 4053 4278 4310 4653 4685 5851
Size: 32 2100 32 225 32 343 32 1166
Cuts at: 5851 5883 6781 6811 6813 6843 574
Size: 32 898 30 2 30 2670
Fragments arranged by size:
2670 2100 1166 898 399 344 343 336
225 108 32 32 32 32 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 5978 5978
Size: 8939
HindIII A'AGCT_T
Cuts at: 1572 1642 3202 1572
Size: 70 1560 7309
Fragments arranged by size:
7309 1560 70
KasI G'GCGC_C
Cuts at: 6369 6617 6369
Size: 248 8691
KpnI G_GTAC'C
Cuts at: 5959 5959
Size: 8939
MfeI C'AATT_G
Cuts at: 1555 1555
Size: 8939
MluI A'CGCG_T
Cuts at: 8372 8372
Size: 8939
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 456 2000 2429 5624 7698 7882 456
Size: 1544 429 3195 2074 184 1513
Fragments arranged by size:
3195 2074 1544 1513 429 184
MscI TGG'CCA
Cuts at: 218 373 2062 2767 2922 5699 6700 218
Size: 155 1689 705 155 2777 1001 2457
Fragments arranged by size:
2777 2457 1689 1001 705 155 155
MslI CAynn'nnrTG
Cuts at: 1969 2246 4610 5270 6928 7055 1969
Size: 277 2364 660 1658 127 3853
Fragments arranged by size:
3853 2364 1658 660 277 127
MspA1I CmG'CkG
Cuts at: 417 2088 3414 6050 6364 6724 7508 7753 417
Size: 1671 1326 2636 314 360 784 245 1603
Fragments arranged by size:
2636 1671 1603 1326 784 360 314 245
NaeI GCC'GGC
Cuts at: 7120 7120
Size: 8939
NarI GG'CG_CC
Cuts at: 6370 6618 6370
Size: 248 8691
NcoI C'CATG_G
Cuts at: 107 227 1987 2504 3742 107
Size: 120 1760 517 1238 5304
Fragments arranged by size:
5304 1760 1238 517 120
NdeI CA'TA_TG
Cuts at: 2247 2973 3399 2247
Size: 726 426 7787
Fragments arranged by size:
7787 726 426
NgoMIV G'CCGG_C
Cuts at: 7118 7118
Size: 8939
NsiI A_TGCA'T
Cuts at: 1289 1289
Size: 8939
NspI r_CATG'y
Cuts at: 149 355 414 1291 2580 5275 5992 6492 8095
Size: 206 59 877 1289 2695 717 500 1603
Cuts at: 8095 149
Size: 993
Fragments arranged by size:
2695 1603 1289 993 877 717 500 206
59
PasI CC'CwG_GG
Cuts at: 1749 4749 1749
Size: 3000 5939
PciI A'CATG_T
Cuts at: 351 8091 351
Size: 7740 1199
PflMI CCAn_nnn'nTGG
Cuts at: 1988 2505 1988
Size: 517 8422
PfoI T'CCnGG_A
Cuts at: 5876 5876
Size: 8939
PmeI GTTT'AAAC
Cuts at: 5938 5938
Size: 8939
PmlI CAC'GTG
Cuts at: 570 570
Size: 8939
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1214 1246 2692 2724 7385 7417 1214
Size: 32 1446 32 4661 32 2736
Fragments arranged by size:
4661 2736 1446 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 4384 5362 4384
Size: 978 7961
PshAI GACnn'nnGTC
Cuts at: 1696 1696
Size: 8939
PsiI TTA'TAA
Cuts at: 2864 2864
Size: 8939
PspOMI G'GGCC_C
Cuts at: 5810 5810
Size: 8939
PspXI vC'TCGA_Gb
Cuts at: 5653 5653
Size: 8939
PstI C_TGCA'G
Cuts at: 2168 3452 4443 5690 5986 6671 2168
Size: 1284 991 1247 296 685 4436
Fragments arranged by size:
4436 1284 1247 991 685 296
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8939
PvuII CAG'CTG
Cuts at: 417 2088 6364 6724 417
Size: 1671 4276 360 2632
Fragments arranged by size:
4276 2632 1671 360
RsrII CG'GwC_CG
Cuts at: 7134 7134
Size: 8939
SacI G_AGCT'C
Cuts at: 5767 5899 5953 5767
Size: 132 54 8753
Fragments arranged by size:
8753 132 54
SalI G'TCGA_C
Cuts at: 5976 5976
Size: 8939
SanDI GG'GwC_CC
Cuts at: 4384 4384
Size: 8939
SapI GCTCTTCn'nnn_
Cuts at: 114 1378 6962 7172 114
Size: 1264 5584 210 1881
Fragments arranged by size:
5584 1881 1264 210
SbfI CC_TGCA'GG
Cuts at: 5986 5986
Size: 8939
ScaI AGT'ACT
Cuts at: 223 1059 1229 2338 8456 223
Size: 836 170 1109 6118 706
Fragments arranged by size:
6118 1109 836 706 170
SexAI A'CCwGG_T
Cuts at: 5364 5364
Size: 8939
SfcI C'TryA_G
Cuts at: 33 291 684 1262 2164 3448 4439 4580 5676
Size: 258 393 578 902 1284 991 141 1096
Cuts at: 5676 5686 5982 6667 7635 7826 33
Size: 10 296 685 968 191 1146
Fragments arranged by size:
1284 1146 1096 991 968 902 685 578
393 296 258 191 141 10
SfoI GGC'GCC
Cuts at: 6371 6619 6371
Size: 248 8691
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8939
SmaI CCC'GGG
Cuts at: 3436 5961 3436
Size: 2525 6414
SmlI C'TyrA_G
Cuts at: 157 184 2349 4827 5438 5653 6094 7009 7723
Size: 27 2165 2478 611 215 441 915 714
Cuts at: 7723 7985 157
Size: 262 1111
Fragments arranged by size:
2478 2165 1111 915 714 611 441 262
215 27
SnaBI TAC'GTA
Cuts at: 3097 3097
Size: 8939
SpeI A'CTAG_T
Cuts at: 67 544 67
Size: 477 8462
SphI G_CATG'C
Cuts at: 414 1291 5992 6492 414
Size: 877 4701 500 2861
Fragments arranged by size:
4701 2861 877 500
SspI AAT'ATT
Cuts at: 1178 1178
Size: 8939
StyI C'CwwG_G
Cuts at: 107 227 374 622 1987 2504 3491 3742 3864
Size: 120 147 248 1365 517 987 251 122
Cuts at: 3864 5889 6073 6427 107
Size: 2025 184 354 2619
Fragments arranged by size:
2619 2025 1365 987 517 354 251 248
184 147 122 120
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2212 2336 2660 3241 3504 3635 3890 5312 5622
Size: 124 324 581 263 131 255 1422 310
Cuts at: 5622 6522 6655 7114 7323 2212
Size: 900 133 459 209 3828
Fragments arranged by size:
3828 1422 900 581 459 324 310 263
255 209 133 131 124
TatI w'GTAC_w
Cuts at: 221 703 1057 1227 1740 2336 2835 3564 4935
Size: 482 354 170 513 596 499 729 1371
Cuts at: 4935 4978 5250 8454 221
Size: 43 272 3204 706
Fragments arranged by size:
3204 1371 729 706 596 513 499 482
354 272 170 43
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 554 586 1064 1096 1670 1702 2066 2098 4943
Size: 32 478 32 574 32 364 32 2845
Cuts at: 4943 4975 5048 5080 8456 8488 554
Size: 32 73 32 3376 32 1005
Fragments arranged by size:
3376 2845 1005 574 478 364 73 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 5366 6736 5366
Size: 1370 7569
XbaI T'CTAG_A
Cuts at: 5970 5970
Size: 8939
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 6434 6434
Size: 8939
XhoI C'TCGA_G
Cuts at: 5653 5653
Size: 8939
XmaI C'CCGG_G
Cuts at: 3434 5959 3434
Size: 2525 6414
XmnI GAAnn'nnTTC
Cuts at: 2390 4549 2390
Size: 2159 6780
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AhdI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NsiI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AgeI AleI AscI BmtI BsiWI BsmBI
BssHII BstEII DraIII EcoNI FseI FspAI HpaI NheI
NotI NruI PacI PsrI SacII SfiI SgrAI SrfI
StuI SwaI ZraI