Restriction map for
pF1KA0055
(Circular) (Six-base) MAPSORT of: pF1KA0055.seq Check: 9884 from: 1 to: 6467
pF1KA0055 6467 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 830 1173 3152 830
Size: 343 1979 4145
Fragments arranged by size:
4145 1979 343
AccI GT'mk_AC
Cuts at: 1039 3505 1039
Size: 2466 4001
Acc65I G'GTAC_C
Cuts at: 3483 3483
Size: 6467
AclI AA'CG_TT
Cuts at: 3144 6307 3144
Size: 3163 3304
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 107 250 476 794 1382 1655 2048 2266 3386
Size: 143 226 318 588 273 393 218 1120
Cuts at: 3386 4292 4724 5471 5739 107
Size: 906 432 747 268 835
Fragments arranged by size:
1120 906 835 747 588 432 393 318
273 268 226 218 143
AfeI AGC'GCT
Cuts at: 2251 2251
Size: 6467
AflIII A'CryG_T
Cuts at: 2347 2920 3819 4923 5900 2347
Size: 573 899 1104 977 2914
Fragments arranged by size:
2914 1104 977 899 573
AgeI A'CCGG_T
Cuts at: 4855 4855
Size: 6467
AleI CACnn'nnGTG
Cuts at: 3287 3287
Size: 6467
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 270 270
Size: 6467
AlwNI CAG_nnn'CTG
Cuts at: 1261 1513 3772 5339 1261
Size: 252 2259 1567 2389
Fragments arranged by size:
2389 2259 1567 252
ApaLI G'TGCA_C
Cuts at: 217 5237 217
Size: 5020 1447
ApoI r'AATT_y
Cuts at: 1943 2559 2593 3471 5820 6076 1943
Size: 616 34 878 2349 256 2334
Fragments arranged by size:
2349 2334 878 616 256 34
AseI AT'TA_AT
Cuts at: 20 20
Size: 6467
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6467
AvaI C'yCGr_G
Cuts at: 3487 3487
Size: 6467
BamHI G'GATC_C
Cuts at: 3492 3492
Size: 6467
BanI G'GyrC_C
Cuts at: 3483 3897 4145 4180 3483
Size: 414 248 35 5770
Fragments arranged by size:
5770 414 248 35
BanII G_rGCy'C
Cuts at: 3078 3481 4511 3078
Size: 403 1030 5034
Fragments arranged by size:
5034 1030 403
BbeI G_GCGC'C
Cuts at: 3901 4149 3901
Size: 248 6219
BbsI GAAGACnn'nnnn_
Cuts at: 181 181
Size: 6467
BbvCI CC'TCA_GC
Cuts at: 1728 1728
Size: 6467
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 6433
BciVI GTATCCnnnnn_n'
Cuts at: 1965 2689 4365 5126 6467 1965
Size: 724 1676 761 1341 1965
Fragments arranged by size:
1965 1676 1341 761 724
BclI T'GATC_A
Cuts at: 2802 3360 3987 2802
Size: 558 627 5282
Fragments arranged by size:
5282 627 558
BglII A'GATC_T
Cuts at: 233 2366 2754 3982 6019 233
Size: 2133 388 1228 2037 681
Fragments arranged by size:
2133 2037 1228 681 388
BlpI GC'TnA_GC
Cuts at: 2219 3579 2219
Size: 1360 5107
Bme1580I G_kGCm'C
Cuts at: 221 228 1874 4092 4185 5241 221
Size: 7 1646 2218 93 1056 1447
Fragments arranged by size:
2218 1646 1447 1056 93 7
BmrI ACTGGGnnnn_n'
Cuts at: 1029 3679 3760 4095 6239 1029
Size: 2650 81 335 2144 1257
Fragments arranged by size:
2650 2144 1257 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4834 4834
Size: 6467
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1229 1229
Size: 6467
Bpu10I CC'TnA_GC
Cuts at: 1052 1728 1052
Size: 676 5791
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 493 2355 3643 4522 5014 5312 493
Size: 1862 1288 879 492 298 1648
Fragments arranged by size:
1862 1648 1288 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 628 2749 6171 628
Size: 2121 3422 924
Fragments arranged by size:
3422 2121 924
BsaAI yAC'GTr
Cuts at: 306 3820 306
Size: 3514 2953
BsaBI GATnn'nnATC
Cuts at: 6 3103 3981 6018 6
Size: 3097 878 2037 455
Fragments arranged by size:
3097 2037 878 455
BsaHI Gr'CG_yC
Cuts at: 1074 1159 3898 4146 4848 6344 1074
Size: 85 2739 248 702 1496 1197
Fragments arranged by size:
2739 1496 1197 702 248 85
BsaWI w'CCGG_w
Cuts at: 461 929 2148 3656 4177 4855 5129 5276 5687
Size: 468 1219 1508 521 678 274 147 411
Cuts at: 5687 461
Size: 1241
Fragments arranged by size:
1508 1241 1219 678 521 468 411 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2301 2331 2301
Size: 30 6437
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1400 2250 1400
Size: 850 5617
BseYI C'CCAG_C
Cuts at: 2069 3892 4650 5227 5723 2069
Size: 1823 758 577 496 2813
Fragments arranged by size:
2813 1823 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2153 2153
Size: 6467
BsiEI CG_ry'CG
Cuts at: 104 4009 4055 5263 104
Size: 3905 46 1208 1308
Fragments arranged by size:
3905 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 221 2025 3078 3481 4259 4449 5241 221
Size: 1804 1053 403 778 190 792 1447
Fragments arranged by size:
1804 1447 1053 792 778 403 190
BsmI GAATG_Cn'
Cuts at: 259 731 259
Size: 472 5995
Bsp1286I G_dGCh'C
Cuts at: 221 228 1874 2025 3078 3481 4092 4185 4259
Size: 7 1646 151 1053 403 611 93 74
Cuts at: 4259 4449 4511 5241 221
Size: 190 62 730 1447
Fragments arranged by size:
1646 1447 1053 730 611 403 190 151
93 74 62 7
BspEI T'CCGG_A
Cuts at: 461 929 5687 461
Size: 468 4758 1241
Fragments arranged by size:
4758 1241 468
BspHI T'CATG_A
Cuts at: 2606 3363 5643 2606
Size: 757 2280 3430
Fragments arranged by size:
3430 2280 757
BspMI ACCTGCnnnn'nnnn_
Cuts at: 830 1155 1173 3152 3517 3731 4033 4414 830
Size: 325 18 1979 365 214 302 381 2883
Fragments arranged by size:
2883 1979 381 365 325 302 214 18
BsrBI CCG'CTC
Cuts at: 50 2223 4759 4813 6297 6462 50
Size: 2173 2536 54 1484 165 55
Fragments arranged by size:
2536 2173 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 615 987 3066 4379 615
Size: 372 2079 1313 2703
Fragments arranged by size:
2703 2079 1313 372
BsrFI r'CCGG_y
Cuts at: 4465 4646 4855 4465
Size: 181 209 6077
Fragments arranged by size:
6077 209 181
BsrGI T'GTAC_A
Cuts at: 2992 2992
Size: 6467
BssSI C'ACGA_G
Cuts at: 3440 4738 5096 3440
Size: 1298 358 4811
Fragments arranged by size:
4811 1298 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1513 2833 3068 3844 1513
Size: 1320 235 776 4136
Fragments arranged by size:
4136 1320 776 235
BstBI TT'CG_AA
Cuts at: 4828 4828
Size: 6467
BstYI r'GATC_y
Cuts at: 1 233 2366 2754 3492 3959 3982 4317 4563
Size: 232 2133 388 738 467 23 335 246
Cuts at: 4563 5575 5661 5673 6019 1
Size: 1012 86 12 346 449
Fragments arranged by size:
2133 1012 738 467 449 388 346 335
246 232 86 23 12
BstZ17I GTA'TAC
Cuts at: 1040 1040
Size: 6467
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4417 4598 4761 4417
Size: 181 163 6123
Fragments arranged by size:
6123 181 163
BtsI GCAGTG_nn'
Cuts at: 797 2491 3710 797
Size: 1694 1219 3554
Fragments arranged by size:
3554 1694 1219
DraI TTT'AAA
Cuts at: 2658 2820 3466 2658
Size: 162 646 5659
Fragments arranged by size:
5659 646 162
DraIII CAC_nnn'GTG
Cuts at: 2029 2029
Size: 6467
DrdI GACnn_nn'nnGTC
Cuts at: 4173 5031 4173
Size: 858 5609
EaeI y'GGCC_r
Cuts at: 4052 4226 4617 4644 5989 4052
Size: 174 391 27 1345 4530
Fragments arranged by size:
4530 1345 391 174 27
EagI C'GGCC_G
Cuts at: 4052 4052
Size: 6467
EarI CTCTTCn'nnn_
Cuts at: 528 847 1079 1358 1523 2288 2293 2773 4490
Size: 319 232 279 165 765 5 480 1717
Cuts at: 4490 4700 528
Size: 210 2295
Fragments arranged by size:
2295 1717 765 480 319 279 232 210
165 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4675 4985 5131 6277 4675
Size: 310 146 1146 4865
Fragments arranged by size:
4865 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3076 3479 3076
Size: 403 6064
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 107 250 476 794 1229 1382 1655 2048 2266
Size: 143 226 318 435 153 273 393 218
Cuts at: 2266 3386 4292 4724 5471 5739 107
Size: 1120 906 432 747 268 835
Fragments arranged by size:
1120 906 835 747 435 432 393 318
273 268 226 218 153 143
EcoO109I rG'GnC_Cy
Cuts at: 281 3606 281
Size: 3325 3142
EcoRI G'AATT_C
Cuts at: 1943 3471 1943
Size: 1528 4939
EcoRV GAT'ATC
Cuts at: 3378 3378
Size: 6467
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 964 2740 4486 964
Size: 1776 1746 2945
Fragments arranged by size:
2945 1776 1746
FspI TGC'GCA
Cuts at: 979 4248 979
Size: 3269 3198
HaeII r_GCGC'y
Cuts at: 2253 3901 4149 5171 6150 2253
Size: 1648 248 1022 979 2570
Fragments arranged by size:
2570 1648 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1191 1223 1390 1422 4309 4339 4341 4371 1191
Size: 32 167 32 2887 30 2 30 3287
Fragments arranged by size:
3287 2887 167 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1478 1859 3506 1478
Size: 381 1647 4439
Fragments arranged by size:
4439 1647 381
HpaI GTT'AAC
Cuts at: 1478 1478
Size: 6467
KasI G'GCGC_C
Cuts at: 3897 4145 3897
Size: 248 6219
KpnI G_GTAC'C
Cuts at: 3487 3487
Size: 6467
MluI A'CGCG_T
Cuts at: 5900 5900
Size: 6467
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 787 1489 1858 2212 2344 5138 5322 787
Size: 702 369 354 132 2794 184 1932
Fragments arranged by size:
2794 1932 702 369 354 184 132
MscI TGG'CCA
Cuts at: 4228 4228
Size: 6467
MslI CAynn'nnrTG
Cuts at: 679 2576 2810 3287 4456 4583 679
Size: 1897 234 477 1169 127 2563
Fragments arranged by size:
2563 1897 1169 477 234 127
MspA1I CmG'CkG
Cuts at: 504 3578 3892 4252 5265 5510 504
Size: 3074 314 360 1013 245 1461
Fragments arranged by size:
3074 1461 1013 360 314 245
NaeI GCC'GGC
Cuts at: 4648 4648
Size: 6467
NarI GG'CG_CC
Cuts at: 3898 4146 3898
Size: 248 6219
NdeI CA'TA_TG
Cuts at: 2138 2138
Size: 6467
NgoMIV G'CCGG_C
Cuts at: 4646 4646
Size: 6467
NspI r_CATG'y
Cuts at: 2351 2836 2924 3520 4020 4927 2351
Size: 485 88 596 500 907 3891
Fragments arranged by size:
3891 907 596 500 485 88
PciI A'CATG_T
Cuts at: 2347 2920 4923 2347
Size: 573 2003 3891
Fragments arranged by size:
3891 2003 573
PflMI CCAn_nnn'nTGG
Cuts at: 1939 2095 1939
Size: 156 6311
PfoI T'CCnGG_A
Cuts at: 789 789
Size: 6467
PmeI GTTT'AAAC
Cuts at: 3466 3466
Size: 6467
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 128 160 5606 5638 128
Size: 32 5446 32 957
Fragments arranged by size:
5446 957 32 32
PsiI TTA'TAA
Cuts at: 317 709 317
Size: 392 6075
PstI C_TGCA'G
Cuts at: 2829 3064 3514 4199 2829
Size: 235 450 685 5097
Fragments arranged by size:
5097 685 450 235
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6467
PvuII CAG'CTG
Cuts at: 3892 4252 3892
Size: 360 6107
RsrII CG'GwC_CG
Cuts at: 4662 4662
Size: 6467
SacI G_AGCT'C
Cuts at: 3078 3481 3078
Size: 403 6064
SalI G'TCGA_C
Cuts at: 3504 3504
Size: 6467
SapI GCTCTTCn'nnn_
Cuts at: 1358 1523 4490 4700 1358
Size: 165 2967 210 3125
Fragments arranged by size:
3125 2967 210 165
SbfI CC_TGCA'GG
Cuts at: 3514 3514
Size: 6467
ScaI AGT'ACT
Cuts at: 5984 5984
Size: 6467
SfcI C'TryA_G
Cuts at: 33 1172 1808 2825 2903 3060 3510 4195 5188
Size: 1139 636 1017 78 157 450 685 993
Cuts at: 5188 5379 33
Size: 191 1121
Fragments arranged by size:
1139 1121 1017 993 685 636 450 191
157 78
SfoI GGC'GCC
Cuts at: 3899 4147 3899
Size: 248 6219
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6467
SmaI CCC'GGG
Cuts at: 3489 3489
Size: 6467
SmlI C'TyrA_G
Cuts at: 472 2370 3622 4537 5029 5291 472
Size: 1898 1252 915 492 262 1648
Fragments arranged by size:
1898 1648 1252 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6467
SphI G_CATG'C
Cuts at: 3520 4020 3520
Size: 500 5967
SspI AAT'ATT
Cuts at: 2480 2480
Size: 6467
StuI AGG'CCT
Cuts at: 2950 2950
Size: 6467
StyI C'CwwG_G
Cuts at: 3601 3955 3601
Size: 354 6113
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1299 2136 4050 4183 4642 4851 1299
Size: 837 1914 133 459 209 2915
Fragments arranged by size:
2915 1914 837 459 209 133
TatI w'GTAC_w
Cuts at: 288 1387 2907 2992 5982 288
Size: 1099 1520 85 2990 773
Fragments arranged by size:
2990 1520 1099 773 85
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5984 6016 5984
Size: 32 6435
Tth111I GACn'n_nGTC
Cuts at: 4264 4264
Size: 6467
XbaI T'CTAG_A
Cuts at: 3498 3498
Size: 6467
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 373 607 3962 114
Size: 259 234 3355 2619
Fragments arranged by size:
3355 2619 259 234
XmaI C'CCGG_G
Cuts at: 3487 3487
Size: 6467
XmnI GAAnn'nnTTC
Cuts at: 1133 2723 1133
Size: 1590 4877
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI BstZ17I BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HpaI KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NdeI
NgoMIV NspI PciI PflMI PfoI PmeI PpiI PsiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AloI ApaI AscI AvrII BaeI
BfrBI BglI BmgBI BmtI BsiWI BsmBI BssHII BstEII
BstXI Bsu36I BtgI ClaI CspCI EcoNI FseI FspAI
HindIII MfeI NcoI NheI NotI NruI NsiI PacI
PasI PmlI PpuMI PshAI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SwaI XhoI
ZraI