Restriction map for
pF1KA0059
(Circular) (Six-base) MAPSORT of: pF1KA0059.seq Check: 9636 from: 1 to: 5267
pF1KA0059 5267 bp
With 182 enzymes: SgfI *
June 20, 2008 10:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 307 814 307
Size: 507 4760
AatII G_ACGT'C
Cuts at: 1725 1725
Size: 5267
AccI GT'mk_AC
Cuts at: 1055 2305 1055
Size: 1250 4017
Acc65I G'GTAC_C
Cuts at: 1241 2283 1241
Size: 1042 4225
AclI AA'CG_TT
Cuts at: 5107 5107
Size: 5267
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2186 3092 3524 3877 4539 2186
Size: 906 432 353 662 2914
Fragments arranged by size:
2914 906 662 432 353
AflIII A'CryG_T
Cuts at: 2054 2619 4419 4700 2054
Size: 565 1800 281 2621
Fragments arranged by size:
2621 1800 565 281
AlwNI CAG_nnn'CTG
Cuts at: 642 2572 4010 642
Size: 1930 1438 1899
Fragments arranged by size:
1930 1899 1438
ApaLI G'TGCA_C
Cuts at: 554 851 4105 554
Size: 297 3254 1716
Fragments arranged by size:
3254 1716 297
ApoI r'AATT_y
Cuts at: 2271 4620 4876 2271
Size: 2349 256 2662
Fragments arranged by size:
2662 2349 256
AseI AT'TA_AT
Cuts at: 20 1747 20
Size: 1727 3540
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5267
AvaI C'yCGr_G
Cuts at: 2287 2287
Size: 5267
BamHI G'GATC_C
Cuts at: 2292 2292
Size: 5267
BanI G'GyrC_C
Cuts at: 1241 1590 2283 2697 2945 2980 1241
Size: 349 693 414 248 35 3528
Fragments arranged by size:
3528 693 414 349 248 35
BanII G_rGCy'C
Cuts at: 137 1291 2281 3311 137
Size: 1154 990 1030 2093
Fragments arranged by size:
2093 1154 1030 990
BbeI G_GCGC'C
Cuts at: 2701 2949 2701
Size: 248 5019
BbsI GAAGACnn'nnnn_
Cuts at: 955 955
Size: 5267
BciVI GTATCCnnnnn_n'
Cuts at: 3165 4221 5267 3165
Size: 1056 1046 3165
Fragments arranged by size:
3165 1056 1046
BclI T'GATC_A
Cuts at: 2787 2787
Size: 5267
BglII A'GATC_T
Cuts at: 1131 1681 2782 4819 1131
Size: 550 1101 2037 1579
Fragments arranged by size:
2037 1579 1101 550
BlpI GC'TnA_GC
Cuts at: 2379 2379
Size: 5267
Bme1580I G_kGCm'C
Cuts at: 558 855 2892 2985 4109 558
Size: 297 2037 93 1124 1716
Fragments arranged by size:
2037 1716 1124 297 93
BmgBI CAC'GTC
Cuts at: 1337 1337
Size: 5267
BmrI ACTGGGnnnn_n'
Cuts at: 2479 2560 2895 5039 2479
Size: 81 335 2144 2707
Fragments arranged by size:
2707 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 376 3634 376
Size: 3258 2009
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 579 838 996 2167 579
Size: 259 158 1171 3679
Fragments arranged by size:
3679 1171 259 158
Bpu10I CC'TnA_GC
Cuts at: 1445 1445
Size: 5267
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2443 3322 4036 4334 2443
Size: 879 714 298 3376
Fragments arranged by size:
3376 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 503 4971 503
Size: 4468 799
BsaAI yAC'GTr
Cuts at: 2620 2620
Size: 5267
BsaBI GATnn'nnATC
Cuts at: 6 2781 4818 6
Size: 2775 2037 455
Fragments arranged by size:
2775 2037 455
BsaHI Gr'CG_yC
Cuts at: 1722 2698 2946 3648 5144 1722
Size: 976 248 702 1496 1845
Fragments arranged by size:
1845 1496 976 702 248
BsaWI w'CCGG_w
Cuts at: 2456 2977 3655 4066 4213 4487 2456
Size: 521 678 411 147 274 3236
Fragments arranged by size:
3236 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 500 530 1203 1233 500
Size: 30 673 30 4534
Fragments arranged by size:
4534 673 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2051 2051
Size: 5267
BseYI C'CCAG_C
Cuts at: 844 1349 1578 1600 2692 3450 4115 4523 844
Size: 505 229 22 1092 758 665 408 1588
Fragments arranged by size:
1588 1092 758 665 505 408 229 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 348 348
Size: 5267
BsiEI CG_ry'CG
Cuts at: 104 1389 2809 2855 4085 104
Size: 1285 1420 46 1230 1286
Fragments arranged by size:
1420 1286 1285 1230 46
BsiHKAI G_wGCw'C
Cuts at: 137 558 738 855 2281 3059 3249 4109 137
Size: 421 180 117 1426 778 190 860 1295
Fragments arranged by size:
1426 1295 860 778 421 190 180 117
BsmBI CGTCTCn'nnnn_
Cuts at: 1615 1713 2220 1615
Size: 98 507 4662
Fragments arranged by size:
4662 507 98
Bsp1286I G_dGCh'C
Cuts at: 137 558 738 855 1291 2281 2892 2985 3059
Size: 421 180 117 436 990 611 93 74
Cuts at: 3059 3249 3311 4109 137
Size: 190 62 798 1295
Fragments arranged by size:
1295 990 798 611 436 421 190 180
117 93 74 62
BspHI T'CATG_A
Cuts at: 3699 3699
Size: 5267
BspMI ACCTGCnnnn'nnnn_
Cuts at: 307 814 2317 2531 2833 3214 307
Size: 507 1503 214 302 381 2360
Fragments arranged by size:
2360 1503 507 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1343 3559 3613 5097 5262 50
Size: 1293 2216 54 1484 165 55
Fragments arranged by size:
2216 1484 1293 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1377 2027 3179 1377
Size: 650 1152 3465
Fragments arranged by size:
3465 1152 650
BsrFI r'CCGG_y
Cuts at: 607 1409 1922 3265 3446 607
Size: 802 513 1343 181 2428
Fragments arranged by size:
2428 1343 802 513 181
BsrGI T'GTAC_A
Cuts at: 1307 1307
Size: 5267
BssSI C'ACGA_G
Cuts at: 1278 3538 4246 1278
Size: 2260 708 2299
Fragments arranged by size:
2299 2260 708
BstAPI GCAn_nnn'nTGC
Cuts at: 642 692 866 2644 642
Size: 50 174 1778 3265
Fragments arranged by size:
3265 1778 174 50
BstBI TT'CG_AA
Cuts at: 3628 3628
Size: 5267
BstEII G'GTnAC_C
Cuts at: 2094 2213 2094
Size: 119 5148
BstXI CCAn_nnnn'nTGG
Cuts at: 232 409 1366 1636 2058 232
Size: 177 957 270 422 3441
Fragments arranged by size:
3441 957 422 270 177
BstYI r'GATC_y
Cuts at: 1 1064 1131 1681 2292 2759 2782 3117 3363
Size: 1063 67 550 611 467 23 335 246
Cuts at: 3363 3669 3681 3767 4819 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1063 1052 611 550 467 449 335 306
246 86 67 23 12
Bsu36I CC'TnA_GG
Cuts at: 182 182
Size: 5267
BtgI C'CryG_G
Cuts at: 155 402 155
Size: 247 5020
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3217 3398 3561 3217
Size: 181 163 4923
Fragments arranged by size:
4923 181 163
BtsI GCAGTG_nn'
Cuts at: 219 667 2510 219
Size: 448 1843 2976
Fragments arranged by size:
2976 1843 448
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 291 326 291
Size: 35 5232
DraI TTT'AAA
Cuts at: 681 2266 681
Size: 1585 3682
DrdI GACnn_nn'nnGTC
Cuts at: 2973 4317 2973
Size: 1344 3923
EaeI y'GGCC_r
Cuts at: 1386 1411 2007 2852 3026 3417 3444 4789 1386
Size: 25 596 845 174 391 27 1345 1864
Fragments arranged by size:
1864 1345 845 596 391 174 27 25
EagI C'GGCC_G
Cuts at: 1386 2852 1386
Size: 1466 3801
EarI CTCTTCn'nnn_
Cuts at: 612 1733 1784 2263 3290 3500 612
Size: 1121 51 479 1027 210 2379
Fragments arranged by size:
2379 1121 1027 479 210 51
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3475 4217 4363 5077 3475
Size: 742 146 714 3665
Fragments arranged by size:
3665 742 714 146
EcoICRI GAG'CTC
Cuts at: 135 2279 135
Size: 2144 3123
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 579 838 996 2167 2186 3092 3524 3877 4539
Size: 259 158 1171 19 906 432 353 662
Cuts at: 4539 579
Size: 1307
Fragments arranged by size:
1307 1171 906 662 432 353 259 158
19
EcoNI CCTnn'n_nnAGG
Cuts at: 2125 2125
Size: 5267
EcoO109I rG'GnC_Cy
Cuts at: 186 702 1449 1663 2406 186
Size: 516 747 214 743 3047
Fragments arranged by size:
3047 747 743 516 214
EcoRI G'AATT_C
Cuts at: 2271 2271
Size: 5267
EcoRV GAT'ATC
Cuts at: 624 1557 624
Size: 933 4334
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3286 3286
Size: 5267
FspI TGC'GCA
Cuts at: 3048 3048
Size: 5267
HaeII r_GCGC'y
Cuts at: 495 643 652 2154 2701 2949 4179 4950 495
Size: 148 9 1502 547 248 1230 771 812
Fragments arranged by size:
1502 1230 812 771 547 248 148 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 444 476 500 532 1203 1235 1254 1286 1763
Size: 32 24 32 671 32 19 32 477
Cuts at: 1763 1795 3109 3139 3141 3171 444
Size: 32 1314 30 2 30 2540
Fragments arranged by size:
2540 1314 671 477 32 32 32 32
32 30 30 24 19 2
HincII GTy'rAC
Cuts at: 547 2306 547
Size: 1759 3508
HindIII A'AGCT_T
Cuts at: 1753 1753
Size: 5267
KasI G'GCGC_C
Cuts at: 2697 2945 2697
Size: 248 5019
KpnI G_GTAC'C
Cuts at: 1245 2287 1245
Size: 1042 4225
MfeI C'AATT_G
Cuts at: 465 600 465
Size: 135 5132
MluI A'CGCG_T
Cuts at: 4700 4700
Size: 5267
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1354 1459 2039 4026 4210 1354
Size: 105 580 1987 184 2411
Fragments arranged by size:
2411 1987 580 184 105
MscI TGG'CCA
Cuts at: 2009 3028 2009
Size: 1019 4248
MslI CAynn'nnrTG
Cuts at: 112 230 407 1463 3256 3383 112
Size: 118 177 1056 1793 127 1996
Fragments arranged by size:
1996 1793 1056 177 127 118
MspA1I CmG'CkG
Cuts at: 381 848 1385 1973 2378 2692 3052 3836 4081
Size: 467 537 588 405 314 360 784 245
Cuts at: 4081 381
Size: 1567
Fragments arranged by size:
1567 784 588 537 467 405 360 314
245
NaeI GCC'GGC
Cuts at: 609 3448 609
Size: 2839 2428
NarI GG'CG_CC
Cuts at: 2698 2946 2698
Size: 248 5019
NcoI C'CATG_G
Cuts at: 155 402 155
Size: 247 5020
NdeI CA'TA_TG
Cuts at: 2079 2079
Size: 5267
NgoMIV G'CCGG_C
Cuts at: 607 3446 607
Size: 2839 2428
NspI r_CATG'y
Cuts at: 1522 2016 2058 2320 2820 4423 1522
Size: 494 42 262 500 1603 2366
Fragments arranged by size:
2366 1603 500 494 262 42
PciI A'CATG_T
Cuts at: 2054 4419 2054
Size: 2365 2902
PflMI CCAn_nnn'nTGG
Cuts at: 920 920
Size: 5267
PfoI T'CCnGG_A
Cuts at: 815 1067 815
Size: 252 5015
PmeI GTTT'AAAC
Cuts at: 2266 2266
Size: 5267
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3713 3745 3713
Size: 32 5235
PpuMI rG'GwC_Cy
Cuts at: 186 702 1663 186
Size: 516 961 3790
Fragments arranged by size:
3790 961 516
PstI C_TGCA'G
Cuts at: 2314 2999 2314
Size: 685 4582
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5267
PvuII CAG'CTG
Cuts at: 848 2692 3052 848
Size: 1844 360 3063
Fragments arranged by size:
3063 1844 360
RsrII CG'GwC_CG
Cuts at: 3462 3462
Size: 5267
SacI G_AGCT'C
Cuts at: 137 2281 137
Size: 2144 3123
SalI G'TCGA_C
Cuts at: 2304 2304
Size: 5267
SapI GCTCTTCn'nnn_
Cuts at: 1733 3290 3500 1733
Size: 1557 210 3500
Fragments arranged by size:
3500 1557 210
SbfI CC_TGCA'GG
Cuts at: 2314 2314
Size: 5267
ScaI AGT'ACT
Cuts at: 4784 4784
Size: 5267
SexAI A'CCwGG_T
Cuts at: 1029 1029
Size: 5267
SfcI C'TryA_G
Cuts at: 33 2310 2995 3963 4154 33
Size: 2277 685 968 191 1146
Fragments arranged by size:
2277 1146 968 685 191
SfoI GGC'GCC
Cuts at: 2699 2947 2699
Size: 248 5019
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5267
SmaI CCC'GGG
Cuts at: 2289 2289
Size: 5267
SmlI C'TyrA_G
Cuts at: 2422 3337 4051 4313 2422
Size: 915 714 262 3376
Fragments arranged by size:
3376 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5267
SphI G_CATG'C
Cuts at: 1522 2320 2820 1522
Size: 798 500 3969
Fragments arranged by size:
3969 798 500
StuI AGG'CCT
Cuts at: 1766 1766
Size: 5267
StyI C'CwwG_G
Cuts at: 119 155 402 1194 1526 2401 2755 119
Size: 36 247 792 332 875 354 2631
Fragments arranged by size:
2631 875 792 354 332 247 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 212 527 967 1262 1518 2048 2850 2983 3442
Size: 315 440 295 256 530 802 133 459
Cuts at: 3442 3651 212
Size: 209 1828
Fragments arranged by size:
1828 802 530 459 440 315 295 256
209 133
TatI w'GTAC_w
Cuts at: 478 716 1307 4782 478
Size: 238 591 3475 963
Fragments arranged by size:
3475 963 591 238
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 646 678 4784 4816 646
Size: 32 4106 32 1097
Fragments arranged by size:
4106 1097 32 32
Tth111I GACn'n_nGTC
Cuts at: 514 1215 3064 514
Size: 701 1849 2717
Fragments arranged by size:
2717 1849 701
XbaI T'CTAG_A
Cuts at: 2298 2298
Size: 5267
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 766 2762 766
Size: 1996 3271
XmaI C'CCGG_G
Cuts at: 2287 2287
Size: 5267
XmnI GAAnn'nnTTC
Cuts at: 257 2171 257
Size: 1914 3353
ZraI GAC'GTC
Cuts at: 1723 1723
Size: 5267
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NspI PciI PflMI PfoI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AgeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmtI
BsiWI BsmI BspEI BssHII BstZ17I ClaI DraIII FseI
FspAI HpaI NheI NotI NruI NsiI PacI PasI
PmlI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI