Restriction map for
pF1KA0060
(Circular) (Six-base) MAPSORT of: pF1KA0060.seq Check: 8638 from: 1 to: 3980
pF1KA0060 3980 bp
With 182 enzymes: SgfI *
June 20, 2008 10:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 481 481
Size: 3980
AccI GT'mk_AC
Cuts at: 443 1018 443
Size: 575 3405
Acc65I G'GTAC_C
Cuts at: 996 996
Size: 3980
AclI AA'CG_TT
Cuts at: 3820 3820
Size: 3980
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1805 2237 2590 3252 1805
Size: 432 353 662 2533
Fragments arranged by size:
2533 662 432 353
AflIII A'CryG_T
Cuts at: 773 1332 3132 3413 773
Size: 559 1800 281 1340
Fragments arranged by size:
1800 1340 559 281
AleI CACnn'nnGTG
Cuts at: 646 646
Size: 3980
AlwNI CAG_nnn'CTG
Cuts at: 1285 2723 1285
Size: 1438 2542
ApaLI G'TGCA_C
Cuts at: 2818 2818
Size: 3980
ApoI r'AATT_y
Cuts at: 984 3333 3589 984
Size: 2349 256 1375
Fragments arranged by size:
2349 1375 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3980
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3980
AvaI C'yCGr_G
Cuts at: 341 1000 341
Size: 659 3321
BamHI G'GATC_C
Cuts at: 1005 1005
Size: 3980
BanI G'GyrC_C
Cuts at: 648 996 1410 1658 1693 648
Size: 348 414 248 35 2935
Fragments arranged by size:
2935 414 348 248 35
BanII G_rGCy'C
Cuts at: 224 994 2024 224
Size: 770 1030 2180
Fragments arranged by size:
2180 1030 770
BbeI G_GCGC'C
Cuts at: 1414 1662 1414
Size: 248 3732
BbsI GAAGACnn'nnnn_
Cuts at: 313 592 862 313
Size: 279 270 3431
Fragments arranged by size:
3431 279 270
BciVI GTATCCnnnnn_n'
Cuts at: 594 1878 2934 3980 594
Size: 1284 1056 1046 594
Fragments arranged by size:
1284 1056 1046 594
BclI T'GATC_A
Cuts at: 1500 1500
Size: 3980
BglII A'GATC_T
Cuts at: 1495 3532 1495
Size: 2037 1943
BlpI GC'TnA_GC
Cuts at: 1092 1092
Size: 3980
Bme1580I G_kGCm'C
Cuts at: 653 682 1605 1698 2822 653
Size: 29 923 93 1124 1811
Fragments arranged by size:
1811 1124 923 93 29
BmrI ACTGGGnnnn_n'
Cuts at: 239 1192 1273 1608 3752 239
Size: 953 81 335 2144 467
Fragments arranged by size:
2144 953 467 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 573 2347 573
Size: 1774 2206
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 145 543 145
Size: 398 3582
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1156 2035 2749 3047 1156
Size: 879 714 298 2089
Fragments arranged by size:
2089 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 338 3684 338
Size: 3346 634
BsaAI yAC'GTr
Cuts at: 330 1333 330
Size: 1003 2977
BsaBI GATnn'nnATC
Cuts at: 6 712 1494 3531 6
Size: 706 782 2037 455
Fragments arranged by size:
2037 782 706 455
BsaHI Gr'CG_yC
Cuts at: 1411 1659 2361 3857 1411
Size: 248 702 1496 1534
Fragments arranged by size:
1534 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1169 1690 2368 2779 2926 3200 1169
Size: 521 678 411 147 274 1949
Fragments arranged by size:
1949 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 772 772
Size: 3980
BseYI C'CCAG_C
Cuts at: 1405 2163 2828 3236 1405
Size: 758 665 408 2149
Fragments arranged by size:
2149 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 1522 1568 2798 104
Size: 1418 46 1230 1286
Fragments arranged by size:
1418 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 131 726 994 1772 1962 2822 131
Size: 595 268 778 190 860 1289
Fragments arranged by size:
1289 860 778 595 268 190
BsmI GAATG_Cn'
Cuts at: 441 441
Size: 3980
Bsp1286I G_dGCh'C
Cuts at: 131 224 653 682 726 994 1605 1698 1772
Size: 93 429 29 44 268 611 93 74
Cuts at: 1772 1962 2024 2822 131
Size: 190 62 798 1289
Fragments arranged by size:
1289 798 611 429 268 190 93 93
74 62 44 29
BspHI T'CATG_A
Cuts at: 2412 2412
Size: 3980
BspMI ACCTGCnnnn'nnnn_
Cuts at: 481 1030 1244 1546 1927 481
Size: 549 214 302 381 2534
Fragments arranged by size:
2534 549 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2272 2326 3810 3975 50
Size: 2222 54 1484 165 55
Fragments arranged by size:
2222 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 536 1892 536
Size: 1356 2624
BsrFI r'CCGG_y
Cuts at: 1978 2159 1978
Size: 181 3799
BsrGI T'GTAC_A
Cuts at: 743 914 743
Size: 171 3809
BssSI C'ACGA_G
Cuts at: 2251 2959 2251
Size: 708 3272
BstAPI GCAn_nnn'nTGC
Cuts at: 1357 1357
Size: 3980
BstBI TT'CG_AA
Cuts at: 955 2341 955
Size: 1386 2594
BstYI r'GATC_y
Cuts at: 1 1005 1472 1495 1830 2076 2382 2394 2480
Size: 1004 467 23 335 246 306 12 86
Cuts at: 2480 3532 1
Size: 1052 449
Fragments arranged by size:
1052 1004 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 596 656 596
Size: 60 3920
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 986 1930 2111 2274 986
Size: 944 181 163 2692
Fragments arranged by size:
2692 944 181 163
BtsI GCAGTG_nn'
Cuts at: 1223 1223
Size: 3980
DraI TTT'AAA
Cuts at: 297 979 297
Size: 682 3298
DrdI GACnn_nn'nnGTC
Cuts at: 1686 3030 1686
Size: 1344 2636
EaeI y'GGCC_r
Cuts at: 593 611 1565 1739 2130 2157 3502 593
Size: 18 954 174 391 27 1345 1071
Fragments arranged by size:
1345 1071 954 391 174 27 18
EagI C'GGCC_G
Cuts at: 1565 1565
Size: 3980
EarI CTCTTCn'nnn_
Cuts at: 467 2003 2213 467
Size: 1536 210 2234
Fragments arranged by size:
2234 1536 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2188 2930 3076 3790 2188
Size: 742 146 714 2378
Fragments arranged by size:
2378 742 714 146
EcoICRI GAG'CTC
Cuts at: 992 992
Size: 3980
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 145 543 1805 2237 2590 3252 145
Size: 398 1262 432 353 662 873
Fragments arranged by size:
1262 873 662 432 398 353
EcoO109I rG'GnC_Cy
Cuts at: 285 575 1119 285
Size: 290 544 3146
Fragments arranged by size:
3146 544 290
EcoRI G'AATT_C
Cuts at: 984 984
Size: 3980
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 968 1999 968
Size: 1031 2949
FspI TGC'GCA
Cuts at: 1761 1761
Size: 3980
HaeII r_GCGC'y
Cuts at: 1414 1662 2892 3663 1414
Size: 248 1230 771 1731
Fragments arranged by size:
1731 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1822 1852 1854 1884 1822
Size: 30 2 30 3918
Fragments arranged by size:
3918 30 30 2
HincII GTy'rAC
Cuts at: 444 1019 444
Size: 575 3405
KasI G'GCGC_C
Cuts at: 1410 1658 1410
Size: 248 3732
KpnI G_GTAC'C
Cuts at: 1000 1000
Size: 3980
MluI A'CGCG_T
Cuts at: 3413 3413
Size: 3980
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 492 2739 2923 492
Size: 2247 184 1549
Fragments arranged by size:
2247 1549 184
MscI TGG'CCA
Cuts at: 595 613 1741 595
Size: 18 1128 2834
Fragments arranged by size:
2834 1128 18
MslI CAynn'nnrTG
Cuts at: 646 685 1969 2096 646
Size: 39 1284 127 2530
Fragments arranged by size:
2530 1284 127 39
MspA1I CmG'CkG
Cuts at: 1091 1405 1765 2549 2794 1091
Size: 314 360 784 245 2277
Fragments arranged by size:
2277 784 360 314 245
NaeI GCC'GGC
Cuts at: 2161 2161
Size: 3980
NarI GG'CG_CC
Cuts at: 1411 1659 1411
Size: 248 3732
NcoI C'CATG_G
Cuts at: 596 656 596
Size: 60 3920
NgoMIV G'CCGG_C
Cuts at: 2159 2159
Size: 3980
NspI r_CATG'y
Cuts at: 777 1033 1533 3136 777
Size: 256 500 1603 1621
Fragments arranged by size:
1621 1603 500 256
PasI CC'CwG_GG
Cuts at: 193 216 193
Size: 23 3957
PciI A'CATG_T
Cuts at: 773 3132 773
Size: 2359 1621
PflMI CCAn_nnn'nTGG
Cuts at: 426 426
Size: 3980
PfoI T'CCnGG_A
Cuts at: 122 571 122
Size: 449 3531
PmeI GTTT'AAAC
Cuts at: 979 979
Size: 3980
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2426 2458 2426
Size: 32 3948
PpuMI rG'GwC_Cy
Cuts at: 285 575 285
Size: 290 3690
PstI C_TGCA'G
Cuts at: 492 1027 1712 492
Size: 535 685 2760
Fragments arranged by size:
2760 685 535
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3980
PvuII CAG'CTG
Cuts at: 1405 1765 1405
Size: 360 3620
RsrII CG'GwC_CG
Cuts at: 2175 2175
Size: 3980
SacI G_AGCT'C
Cuts at: 994 994
Size: 3980
SalI G'TCGA_C
Cuts at: 442 1017 442
Size: 575 3405
SapI GCTCTTCn'nnn_
Cuts at: 2003 2213 2003
Size: 210 3770
SbfI CC_TGCA'GG
Cuts at: 1027 1027
Size: 3980
ScaI AGT'ACT
Cuts at: 208 3497 208
Size: 3289 691
SfcI C'TryA_G
Cuts at: 33 448 488 1023 1708 2676 2867 33
Size: 415 40 535 685 968 191 1146
Fragments arranged by size:
1146 968 685 535 415 191 40
SfoI GGC'GCC
Cuts at: 1412 1660 1412
Size: 248 3732
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3980
SmaI CCC'GGG
Cuts at: 1002 1002
Size: 3980
SmlI C'TyrA_G
Cuts at: 341 1135 2050 2764 3026 341
Size: 794 915 714 262 1295
Fragments arranged by size:
1295 915 794 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3980
SphI G_CATG'C
Cuts at: 1033 1533 1033
Size: 500 3480
StyI C'CwwG_G
Cuts at: 596 644 656 839 1114 1468 596
Size: 48 12 183 275 354 3108
Fragments arranged by size:
3108 354 275 183 48 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 422 433 1563 1696 2155 2364 422
Size: 11 1130 133 459 209 2038
Fragments arranged by size:
2038 1130 459 209 133 11
TatI w'GTAC_w
Cuts at: 206 743 914 3495 206
Size: 537 171 2581 691
Fragments arranged by size:
2581 691 537 171
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3497 3529 3497
Size: 32 3948
Tth111I GACn'n_nGTC
Cuts at: 783 1777 783
Size: 994 2986
XbaI T'CTAG_A
Cuts at: 1011 1011
Size: 3980
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 427 1475 427
Size: 1048 2932
XhoI C'TCGA_G
Cuts at: 341 341
Size: 3980
XmaI C'CCGG_G
Cuts at: 1000 1000
Size: 3980
XmnI GAAnn'nnTTC
Cuts at: 208 310 382 208
Size: 102 72 3806
Fragments arranged by size:
3806 102 72
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BsgI BsiWI BsmBI BspEI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI ZraI