Restriction map for
pF1KA0068
(Circular) (Six-base) MAPSORT of: pF1KA0068.seq Check: 7363 from: 1 to: 6872
pF1KA0068 6872 bp
With 182 enzymes: SgfI *
June 20, 2008 10:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1482 1482
Size: 6872
AccI GT'mk_AC
Cuts at: 3518 3910 3518
Size: 392 6480
Acc65I G'GTAC_C
Cuts at: 2549 3888 2549
Size: 1339 5533
AclI AA'CG_TT
Cuts at: 6712 6712
Size: 6872
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 649 1424 2377 2929 3067 3094 3805 4697 5129
Size: 775 953 552 138 27 711 892 432
Cuts at: 5129 5876 6144 649
Size: 747 268 1377
Fragments arranged by size:
1377 953 892 775 747 711 552 432
268 138 27
AfeI AGC'GCT
Cuts at: 1401 1401
Size: 6872
AflIII A'CryG_T
Cuts at: 605 2600 4224 5328 6305 605
Size: 1995 1624 1104 977 1172
Fragments arranged by size:
1995 1624 1172 1104 977
AgeI A'CCGG_T
Cuts at: 2693 5260 2693
Size: 2567 4305
AhdI GACnn_n'nnGTC
Cuts at: 138 564 138
Size: 426 6446
AleI CACnn'nnGTG
Cuts at: 3811 3811
Size: 6872
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2768 2959 2768
Size: 191 6681
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1504 1536 1504
Size: 32 6840
AlwNI CAG_nnn'CTG
Cuts at: 735 777 1299 1497 2166 2769 3096 3147 4177
Size: 42 522 198 669 603 327 51 1030
Cuts at: 4177 5744 735
Size: 1567 1863
Fragments arranged by size:
1863 1567 1030 669 603 522 327 198
51 42
ApaI G_GGCC'C
Cuts at: 1868 1868
Size: 6872
ApaLI G'TGCA_C
Cuts at: 1107 1357 5642 1107
Size: 250 4285 2337
Fragments arranged by size:
4285 2337 250
ApoI r'AATT_y
Cuts at: 228 477 595 1888 1926 2728 3728 3876 6225
Size: 249 118 1293 38 802 1000 148 2349
Cuts at: 6225 6481 228
Size: 256 619
Fragments arranged by size:
2349 1293 1000 802 619 256 249 148
118 38
AseI AT'TA_AT
Cuts at: 20 20
Size: 6872
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6872
AvaI C'yCGr_G
Cuts at: 377 1230 3892 377
Size: 853 2662 3357
Fragments arranged by size:
3357 2662 853
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2865 2898 2865
Size: 33 6839
BamHI G'GATC_C
Cuts at: 2012 2045 2776 3404 3897 2012
Size: 33 731 628 493 4987
Fragments arranged by size:
4987 731 628 493 33
BanI G'GyrC_C
Cuts at: 530 2549 3888 4302 4550 4585 530
Size: 2019 1339 414 248 35 2817
Fragments arranged by size:
2817 2019 1339 414 248 35
BanII G_rGCy'C
Cuts at: 200 818 1231 1688 1868 2648 3886 4916 200
Size: 618 413 457 180 780 1238 1030 2156
Fragments arranged by size:
2156 1238 1030 780 618 457 413 180
BbeI G_GCGC'C
Cuts at: 534 4306 4554 534
Size: 3772 248 2852
Fragments arranged by size:
3772 2852 248
BbsI GAAGACnn'nnnn_
Cuts at: 254 1258 1660 2628 2962 3082 254
Size: 1004 402 968 334 120 4044
Fragments arranged by size:
4044 1004 968 402 334 120
BbvCI CC'TCA_GC
Cuts at: 3314 3314
Size: 6872
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1088 1122 1260 1294 2279 2313 1088
Size: 34 138 34 985 34 5647
Fragments arranged by size:
5647 985 138 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3031 4770 5531 6872 3031
Size: 1739 761 1341 3031
Fragments arranged by size:
3031 1739 1341 761
BclI T'GATC_A
Cuts at: 581 1478 2408 3596 4392 581
Size: 897 930 1188 796 3061
Fragments arranged by size:
3061 1188 930 897 796
BfrBI ATG'CAT
Cuts at: 259 3465 259
Size: 3206 3666
BglI GCCn_nnn'nGGC
Cuts at: 112 1596 112
Size: 1484 5388
BglII A'GATC_T
Cuts at: 2981 4387 6424 2981
Size: 1406 2037 3429
Fragments arranged by size:
3429 2037 1406
BlpI GC'TnA_GC
Cuts at: 2542 3984 2542
Size: 1442 5430
Bme1580I G_kGCm'C
Cuts at: 1111 1361 1868 3808 4497 4590 5646 1111
Size: 250 507 1940 689 93 1056 2337
Fragments arranged by size:
2337 1940 1056 689 507 250 93
BmgBI CAC'GTC
Cuts at: 1106 2634 1106
Size: 1528 5344
BmrI ACTGGGnnnn_n'
Cuts at: 598 872 1677 3313 3591 4084 4165 4500 6644
Size: 274 805 1636 278 493 81 335 2144
Cuts at: 6644 598
Size: 826
Fragments arranged by size:
2144 1636 826 805 493 335 278 274
81
BmtI G_CTAG'C
Cuts at: 2227 2227
Size: 6872
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 698 3027 5239 698
Size: 2329 2212 2331
Fragments arranged by size:
2331 2329 2212
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 148 178 346 384 472 997 1755 2011 2086
Size: 30 168 38 88 525 758 256 75
Cuts at: 2086 2787 3046 148
Size: 701 259 3974
Fragments arranged by size:
3974 758 701 525 259 256 168 88
75 38 30
Bpu10I CC'TnA_GC
Cuts at: 3314 3314
Size: 6872
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1866 1879 4048 4927 5419 5717 1866
Size: 13 2169 879 492 298 3021
Fragments arranged by size:
3021 2169 879 492 298 13
BsaI GGTCTCn'nnnn_
Cuts at: 2062 6576 2062
Size: 4514 2358
BsaAI yAC'GTr
Cuts at: 694 1329 2097 2952 4225 694
Size: 635 768 855 1273 3341
Fragments arranged by size:
3341 1273 855 768 635
BsaBI GATnn'nnATC
Cuts at: 6 1159 4386 6423 6
Size: 1153 3227 2037 455
Fragments arranged by size:
3227 2037 1153 455
BsaHI Gr'CG_yC
Cuts at: 531 874 2573 4303 4551 5253 6749 531
Size: 343 1699 1730 248 702 1496 654
Fragments arranged by size:
1730 1699 1496 702 654 343 248
BsaWI w'CCGG_w
Cuts at: 2693 2822 3407 4061 4582 5260 5534 5681 6092
Size: 129 585 654 521 678 274 147 411
Cuts at: 6092 2693
Size: 3473
Fragments arranged by size:
3473 678 654 585 521 411 274 147
129
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 927 957 1360 1390 1504 1534 927
Size: 30 403 30 114 30 6265
Fragments arranged by size:
6265 403 114 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 175 343 381 564 1459 2368 2914 175
Size: 168 38 183 895 909 546 4133
Fragments arranged by size:
4133 909 895 546 183 168 38
BseYI C'CCAG_C
Cuts at: 1698 2414 3448 4297 5055 5632 6128 1698
Size: 716 1034 849 758 577 496 2442
Fragments arranged by size:
2442 1034 849 758 716 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 663 2392 2614 3160 663
Size: 1729 222 546 4375
Fragments arranged by size:
4375 1729 546 222
BsiEI CG_ry'CG
Cuts at: 104 4414 4460 5668 104
Size: 4310 46 1208 1308
Fragments arranged by size:
4310 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 818 1111 1361 2102 2648 3886 4664 4854 5646
Size: 293 250 741 546 1238 778 190 792
Cuts at: 5646 818
Size: 2044
Fragments arranged by size:
2044 1238 792 778 741 546 293 250
190
BsmI GAATG_Cn'
Cuts at: 2302 3524 2302
Size: 1222 5650
BsmBI CGTCTCn'nnnn_
Cuts at: 1666 2341 2427 1666
Size: 675 86 6111
Fragments arranged by size:
6111 675 86
Bsp1286I G_dGCh'C
Cuts at: 200 818 1111 1231 1361 1688 1868 2102 2648
Size: 618 293 120 130 327 180 234 546
Cuts at: 2648 3808 3886 4497 4590 4664 4854 4916 5646
Size: 1160 78 611 93 74 190 62 730
Cuts at: 5646 200
Size: 1426
Fragments arranged by size:
1426 1160 730 618 611 546 327 293
234 190 180 130 120 93 78 74
62
BspEI T'CCGG_A
Cuts at: 3407 6092 3407
Size: 2685 4187
BspHI T'CATG_A
Cuts at: 6048 6048
Size: 6872
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1482 3104 3179 3353 3922 4136 4438 4819 1482
Size: 1622 75 174 569 214 302 381 3535
Fragments arranged by size:
3535 1622 569 381 302 214 174 75
BsrBI CCG'CTC
Cuts at: 50 682 1730 5164 5218 6702 6867 50
Size: 632 1048 3434 54 1484 165 55
Fragments arranged by size:
3434 1484 1048 632 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1814 3459 4784 1814
Size: 1645 1325 3902
Fragments arranged by size:
3902 1645 1325
BsrFI r'CCGG_y
Cuts at: 2546 2693 2861 4870 5051 5260 2546
Size: 147 168 2009 181 209 4158
Fragments arranged by size:
4158 2009 209 181 168 147
BsrGI T'GTAC_A
Cuts at: 356 2117 356
Size: 1761 5111
BssHII G'CGCG_C
Cuts at: 1198 1322 1198
Size: 124 6748
BssSI C'ACGA_G
Cuts at: 3001 3541 5143 5501 3001
Size: 540 1602 358 4372
Fragments arranged by size:
4372 1602 540 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1299 4249 1299
Size: 2950 3922
BstBI TT'CG_AA
Cuts at: 5233 5233
Size: 6872
BstEII G'GTnAC_C
Cuts at: 1575 1575
Size: 6872
BstXI CCAn_nnnn'nTGG
Cuts at: 747 1875 747
Size: 1128 5744
BstYI r'GATC_y
Cuts at: 1 705 1160 2012 2045 2776 2981 3404 3897
Size: 704 455 852 33 731 205 423 493
Cuts at: 3897 4364 4387 4722 4968 5980 6066 6078 6424
Size: 467 23 335 246 1012 86 12 346
Cuts at: 6424 1
Size: 449
Fragments arranged by size:
1012 852 731 704 493 467 455 449
423 346 335 246 205 86 33 23
12
Bsu36I CC'TnA_GG
Cuts at: 458 1582 458
Size: 1124 5748
BtgI C'CryG_G
Cuts at: 107 440 1760 2000 2888 3530 107
Size: 333 1320 240 888 642 3449
Fragments arranged by size:
3449 1320 888 642 333 240
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 258 3312 4822 5003 5166 258
Size: 3054 1510 181 163 1964
Fragments arranged by size:
3054 1964 1510 181 163
BtsI GCAGTG_nn'
Cuts at: 651 1321 1555 1954 3565 4115 651
Size: 670 234 399 1611 550 3408
Fragments arranged by size:
3408 1611 670 550 399 234
ClaI AT'CG_AT
Cuts at: 2083 2083
Size: 6872
DraI TTT'AAA
Cuts at: 3871 3871
Size: 6872
DraIII CAC_nnn'GTG
Cuts at: 2601 2601
Size: 6872
DrdI GACnn_nn'nnGTC
Cuts at: 4578 5436 4578
Size: 858 6014
EaeI y'GGCC_r
Cuts at: 749 4457 4631 5022 5049 6394 749
Size: 3708 174 391 27 1345 1227
Fragments arranged by size:
3708 1345 1227 391 174 27
EagI C'GGCC_G
Cuts at: 4457 4457
Size: 6872
EarI CTCTTCn'nnn_
Cuts at: 806 1282 1400 3127 4895 5105 806
Size: 476 118 1727 1768 210 2573
Fragments arranged by size:
2573 1768 1727 476 210 118
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5080 5390 5536 6682 5080
Size: 310 146 1146 5270
Fragments arranged by size:
5270 1146 310 146
EcoICRI GAG'CTC
Cuts at: 816 2646 3884 816
Size: 1830 1238 3804
Fragments arranged by size:
3804 1830 1238
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 148 178 346 384 472 649 997 1424 1755
Size: 30 168 38 88 177 348 427 331
Cuts at: 1755 2011 2086 2377 2787 2929 3046 3067 3094
Size: 256 75 291 410 142 117 21 27
Cuts at: 3094 3805 4697 5129 5876 6144 148
Size: 711 892 432 747 268 876
Fragments arranged by size:
892 876 747 711 432 427 410 348
331 291 268 256 177 168 142 117
88 75 38 30 27 21
EcoNI CCTnn'n_nnAGG
Cuts at: 157 1462 2616 3100 157
Size: 1305 1154 484 3929
Fragments arranged by size:
3929 1305 1154 484
EcoO109I rG'GnC_Cy
Cuts at: 1296 1719 1765 1864 2836 3308 3342 4011 1296
Size: 423 46 99 972 472 34 669 4157
Fragments arranged by size:
4157 972 669 472 423 99 46 34
EcoRI G'AATT_C
Cuts at: 3728 3876 3728
Size: 148 6724
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1749 1860 4891 1749
Size: 111 3031 3730
Fragments arranged by size:
3730 3031 111
FspI TGC'GCA
Cuts at: 4653 4653
Size: 6872
HaeII r_GCGC'y
Cuts at: 534 1076 1291 1403 2132 2611 3363 4306 4554
Size: 542 215 112 729 479 752 943 248
Cuts at: 4554 5576 6555 534
Size: 1022 979 851
Fragments arranged by size:
1022 979 943 851 752 729 542 479
248 215 112
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 124 156 410 442 550 582 697 729 1360
Size: 32 254 32 108 32 115 32 631
Cuts at: 1360 1392 2303 2335 2470 2502 4714 4744 4746
Size: 32 911 32 135 32 2212 30 2
Cuts at: 4746 4776 124
Size: 30 2220
Fragments arranged by size:
2220 2212 911 631 254 135 115 108
32 32 32 32 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 3911 3911
Size: 6872
HindIII A'AGCT_T
Cuts at: 1351 1351
Size: 6872
KasI G'GCGC_C
Cuts at: 530 4302 4550 530
Size: 3772 248 2852
Fragments arranged by size:
3772 2852 248
KpnI G_GTAC'C
Cuts at: 2553 3892 2553
Size: 1339 5533
MluI A'CGCG_T
Cuts at: 6305 6305
Size: 6872
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 167 938 1403 2492 5543 5727 167
Size: 771 465 1089 3051 184 1312
Fragments arranged by size:
3051 1312 1089 771 465 184
MscI TGG'CCA
Cuts at: 751 4633 751
Size: 3882 2990
MslI CAynn'nnrTG
Cuts at: 604 2029 3466 3529 3811 4861 4988 604
Size: 1425 1437 63 282 1050 127 2488
Fragments arranged by size:
2488 1437 1425 1050 282 127 63
MspA1I CmG'CkG
Cuts at: 1305 1593 1968 3045 3276 3621 3983 4297 4657
Size: 288 375 1077 231 345 362 314 360
Cuts at: 4657 5670 5915 1305
Size: 1013 245 2262
Fragments arranged by size:
2262 1077 1013 375 362 360 345 314
288 245 231
NaeI GCC'GGC
Cuts at: 2863 5053 2863
Size: 2190 4682
NarI GG'CG_CC
Cuts at: 531 4303 4551 531
Size: 3772 248 2852
Fragments arranged by size:
3772 2852 248
NcoI C'CATG_G
Cuts at: 107 2000 107
Size: 1893 4979
NgoMIV G'CCGG_C
Cuts at: 2861 5051 2861
Size: 2190 4682
NheI G'CTAG_C
Cuts at: 2223 2223
Size: 6872
NsiI A_TGCA'T
Cuts at: 261 3467 261
Size: 3206 3666
NspI r_CATG'y
Cuts at: 609 894 1041 1179 2371 3469 3823 3925 4425
Size: 285 147 138 1192 1098 354 102 500
Cuts at: 4425 5332 609
Size: 907 2149
Fragments arranged by size:
2149 1192 1098 907 500 354 285 147
138 102
PciI A'CATG_T
Cuts at: 605 5328 605
Size: 4723 2149
PflMI CCAn_nnn'nTGG
Cuts at: 2064 2064
Size: 6872
PfoI T'CCnGG_A
Cuts at: 155 719 1345 1988 2063 2582 3023 3770 155
Size: 564 626 643 75 519 441 747 3257
Fragments arranged by size:
3257 747 643 626 564 519 441 75
PmeI GTTT'AAAC
Cuts at: 3871 3871
Size: 6872
PmlI CAC'GTG
Cuts at: 1329 1329
Size: 6872
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 646 678 1504 1536 6011 6043 646
Size: 32 826 32 4475 32 1475
Fragments arranged by size:
4475 1475 826 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 1719 1765 2836 3308 3342 1719
Size: 46 1071 472 34 5249
Fragments arranged by size:
5249 1071 472 46 34
PsiI TTA'TAA
Cuts at: 2249 2249
Size: 6872
PspOMI G'GGCC_C
Cuts at: 1864 1864
Size: 6872
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2782 2814 2782
Size: 32 6840
PstI C_TGCA'G
Cuts at: 782 1295 1304 1493 1562 1643 1946 2948 3919
Size: 513 9 189 69 81 303 1002 971
Cuts at: 3919 4604 782
Size: 685 3050
Fragments arranged by size:
3050 1002 971 685 513 303 189 81
69 9
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6872
PvuII CAG'CTG
Cuts at: 1305 1968 3045 4297 4657 1305
Size: 663 1077 1252 360 3520
Fragments arranged by size:
3520 1252 1077 663 360
RsrII CG'GwC_CG
Cuts at: 5067 5067
Size: 6872
SacI G_AGCT'C
Cuts at: 818 2648 3886 818
Size: 1830 1238 3804
Fragments arranged by size:
3804 1830 1238
SalI G'TCGA_C
Cuts at: 3909 3909
Size: 6872
SanDI GG'GwC_CC
Cuts at: 1765 3308 1765
Size: 1543 5329
SapI GCTCTTCn'nnn_
Cuts at: 806 1282 4895 5105 806
Size: 476 3613 210 2573
Fragments arranged by size:
3613 2573 476 210
SbfI CC_TGCA'GG
Cuts at: 1493 1643 3919 1493
Size: 150 2276 4446
Fragments arranged by size:
4446 2276 150
ScaI AGT'ACT
Cuts at: 1000 1375 6389 1000
Size: 375 5014 1483
Fragments arranged by size:
5014 1483 375
SexAI A'CCwGG_T
Cuts at: 2146 2146
Size: 6872
SfcI C'TryA_G
Cuts at: 33 778 1203 1291 1300 1489 1558 1639 1942
Size: 745 425 88 9 189 69 81 303
Cuts at: 1942 2944 3915 4600 5593 5784 33
Size: 1002 971 685 993 191 1121
Fragments arranged by size:
1121 1002 993 971 745 685 425 303
191 189 88 81 69 9
SfoI GGC'GCC
Cuts at: 532 4304 4552 532
Size: 3772 248 2852
Fragments arranged by size:
3772 2852 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6872
SmaI CCC'GGG
Cuts at: 379 3894 379
Size: 3515 3357
SmlI C'TyrA_G
Cuts at: 1845 1858 4027 4942 5434 5696 1845
Size: 13 2169 915 492 262 3021
Fragments arranged by size:
3021 2169 915 492 262 13
SnaBI TAC'GTA
Cuts at: 694 694
Size: 6872
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6872
SphI G_CATG'C
Cuts at: 3925 4425 3925
Size: 500 6372
StuI AGG'CCT
Cuts at: 1487 1487
Size: 6872
StyI C'CwwG_G
Cuts at: 107 2000 2072 2780 4006 4360 107
Size: 1893 72 708 1226 354 2619
Fragments arranged by size:
2619 1893 1226 708 354 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2428 4455 4588 5047 5256 2428
Size: 2027 133 459 209 4044
Fragments arranged by size:
4044 2027 459 209 133
TatI w'GTAC_w
Cuts at: 356 485 998 1133 1373 2117 6387 356
Size: 129 513 135 240 744 4270 841
Fragments arranged by size:
4270 841 744 513 240 135 129
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 701 733 6389 6421 701
Size: 32 5656 32 1152
Fragments arranged by size:
5656 1152 32 32
Tth111I GACn'n_nGTC
Cuts at: 4669 4669
Size: 6872
XbaI T'CTAG_A
Cuts at: 3903 3903
Size: 6872
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2496 3673 4367 2496
Size: 1177 694 5001
Fragments arranged by size:
5001 1177 694
XmaI C'CCGG_G
Cuts at: 377 3892 377
Size: 3515 3357
XmnI GAAnn'nnTTC
Cuts at: 559 1889 559
Size: 1330 5542
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI
NsiI NspI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PsiI PspOMI PsrI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SnaBI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AflII AscI AvrII BsiWI BstZ17I CspCI EcoRV
FseI FspAI HpaI MfeI NdeI NotI NruI PacI
PasI PshAI PspXI SacII SfiI SgrAI SrfI SspI
SwaI XhoI ZraI